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| 1 | Efficient RT-QuIC seeding activity for α-synuclein in olfactory mucosa samples of patients with Parkinson’s disease and multiple system atrophy显示文摘Background:Parkinson’s disease(PD)is a neurodegenerative disorder whose diagnosis is often challenging because symptoms may overlap with neurodegenerative parkinsonisms.PD is characterized by intraneuronal accumulation of abnormalα-synuclein in brainstem while neurodegenerative parkinsonisms might be associated with accumulation of eitherα-synuclein,as in the case of Multiple System Atrophy(MSA)or tau,as in the case of Corticobasal Degeneration(CBD)and Progressive Supranuclear Palsy(PSP),in other disease-specific brain regions.Definite diagnosis of all these diseases can be formulated only neuropathologically by detection and localization ofα-synuclein or tau aggregates in the brain.Compelling evidence suggests that trace-amount of these proteins can appear in peripheral tissues,including receptor neurons of the olfactory mucosa(OM).Methods:We have set and standardized the experimental conditions to extend the ultrasensitive Real Time Quaking Induced Conversion(RT-QuIC)assay for OM analysis.In particular,by using human recombinantα-synuclein as substrate of reaction,we have assessed the ability of OM collected from patients with clinical diagnoses of PD and MSA to induceα-synuclein aggregation,and compared their seeding ability to that of OM samples collected from patients with clinical diagnoses of CBD and PSP.Results:Our results showed that a significant percentage of MSA and PD samples inducedα-synuclein aggregation with high efficiency,but also few samples of patients with the clinical diagnosis of CBD and PSP caused the same effect.Notably,the final RT-QuIC aggregates obtained from MSA and PD samples owned peculiar biochemical and morphological features potentially enabling their discrimination.Conclusions:Our study provide the proof-of-concept that olfactory mucosa samples collected from patients with PD and MSA possess important seeding activities forα-synuclein.Additional studies are required for(i)estimating sensitivity and specificity of the technique and for(ii)evaluating its application for the diagnosis of PD and neurodegenerative parkinsonisms.RT-QuIC analyses of OM and cerebrospinal fluid(CSF)can be combined with the aim of increasing the overall diagnostic accuracy of these diseases,especially in the early stages. | Chiara Maria Giulia De Luca Antonio Emanuele Elia Sara Maria Portaleone Federico Angelo Cazzaniga Martina Rossi Edoardo Bistaffa Elena De Cecco Joanna Narkiewicz Giulia Salzano Olga Carletta Luigi Romito Grazia Devigili Paola Soliveri Pietro Tiraboschi Giuseppe Legname Fabrizio Tagliavini Roberto Eleopra Giorgio Giaccone Fabio Moda | 2019 | Translational Neurodegeneration2019，8，1： | 5 |
| 2 | Synthesis of sugar esters in solvent mictures by lipases from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties显示文摘 | Manuel Ferrer Juan Soliveri | 2005 | EnzymeMicrobTechnol2005，36，： | 1 |
| 3 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and candida Antarctica B,and their antimicrobial properties显示文摘 | Ferrer M Soliveri J Plan F J | 2005 | Enzyme and Microbial Technology2005，36，4： | 1 |
| 4 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyceslanuginosus and Candida antarctica B,and their antimicrobial properties显示文摘 | Ferrer M Soliveri J Plou F J | 2005 | Enzyme and Microbial Technology2005，36，4： | 1 |
| 5 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties显示文摘 | FERRER M SOLIVERI J PLOU F J | 2005 | Enzym and Miero Tech2005，36，： | 1 |
| 6 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida Antarctica B, and their antimicrobial properties 显示文摘 | FERRER M SOLIVERI J PLOU F J | 2005 | Enzyme and Microbial Technology2005，36，： | 1 |
| 7 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B and their antimicrobial properties显示文摘 | Ferrer M Soliveri J Plou F | 2005 | Enzyme and Microbial Technology2005，36，4： | 1 |
| 8 | Synthesis of sugar esters in solvent mixtures by lipase from Thermomyces lanuginosus and Candida antarctica B,and their antimicrobial properties显示文摘 | Ferrer M Soliveri J Plou J F | 2005 | Enzyme Microb Technol2005，36，： | 1 |
| 9 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanug- inosus and Candida antarctica B, and their anttmtCrobla显示文摘 | FERRER M SOLIVERI J PLOU | 2005 | Enzym and Micro Tech2005，36，： | 1 |
| 10 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B,and their antimicrobial properties显示文摘 | M Ferrer J Soliveri F J Plou | | 0，，： | 1 |
| 11 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyees lanuginosus and Candida antarctica B, sand their antimicrobial properties 显示文摘 | FERRER M SOLIVERI J PLOU F J | 2005 | Enzyme Microb Techno12005，36，4： | 1 |
| 12 | Synthesis of sugar esters in solvent mixtures by lipase from Thermomyces lanuginosus and Candida antarctica B, and their antimicrobial properties 显示文摘 | Ferrer M Soliveri J Plou J F | 2005 | Enzyme Microb Technol2005，36，4： | 1 |
| 13 | Synthesis of sugar esters in solvent mixtures by llpases from thermomyces lanuginosus and candida antarctica B,and their antimicrbial properties显示文摘 | FERRER M SOLIVERI J PLOU F J | 2005 | Enzyme and Microbial Technology2005，36，4： | 1 |
| 14 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida Antarctica B,and their antimicrobial properties显示文摘 | FERRER M SOLIVERI J PLOU F J | 2005 | Enzyme and Microbial Technology2005，36，： | 1 |
| 15 | Reduced risk factors for vascular disorders in Parkinson disease patients:a case-control stud y 显示文摘 | Scigliano G Musieco M Soliveri P | 2006 | Stroke2006，37，5： | 1 |
| 16 | Dementia and cognitive impairment in Parkinson's disease 显示文摘 | Soliveri P Carella F | 1988 | J Neurol Neurosurg Psychiatry1988，51，： | 1 |
| 17 | Cognitive and behavioral disturbances in Parkinsons disease显示文摘 | Girotti F Soliveri P | 2003 | Neurol Sci2003，24，： | 1 |
| 18 | Synthesis of sugar esters in solvent mixtures by lipases from Thermomyces lanuginosus and Candida antarctica B,and their antimierobial properties 显示文摘 | Ferrer M Soliveri J Plou J F | 2005 | Enzyme and Microb Technol2005，36，4： | 1 |
| 19 | Synthesis of sugar esters in solvent mictures by lipases from thermomyces lanuginosus and candida antarctica B,and their antimicrobial properties显示文摘 | Manuel Ferrer Juan Soliveri | 2005 | Enzyme and Microblal Technology2005，36，： | 1 |
| 20 | Synthesis of sug- ar esters in solvent mixtures by lipases from Thermomy- ceslanuginosus and Candida antarcticab, and their antimi- crobial properties 显示文摘 | FERRER M SOLIVERI J PLOU F | 2005 | Enzyme Microb Technol2005，36，4： | 1 |
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