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| 1 | PI3K/Akt signaling transduction pathway is involved in rat vascular smooth muscle cell proliferation induced by apelin-13显示文摘脉管的光滑的肌肉房间(VSMC ) 被将生物的活组织移植于培养基中培养方法从男 SpragueDawley 老鼠的胸的主动脉准备经由 3 kinase (PI3K )/Akt 发信号 transduction 小径由 apelin-13 导致了的 phosphoinositide 观察 VSMC 增长。PI3K, phospho-PI3K, phospho-Akt, ERK1/2, phospho-ERK1/2 和 cyclin D1 的表示被西方的污点分析检测。结果证明 apelin-13 在剂量依赖者和时间依赖者举止支持了 phospho-PI3K 和 phospho-Akt 的表示。PI3K 禁止者 LY294002 显著地减少了 phospho-PI3K, phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 导致了。Akt 禁止者 1701-1 显著地减少了 phospho-Akt, phospho-ERK1/2,和 cyclin D1 的表示由 apelin-13 刺激了。MTT 试金结果证明 PI3K 禁止者 LY294002 和 Akt 禁止者 1701-1 显著地禁止了 apelin-13 导致的 VSMC 增长。Apelin-13 通过表明 transduction 小径的 PI3K/Akt 支持了 VSMC 增长。 | Changhui Liu Tao Su Fang Li Lanfang Li Xuping Qin Weinan Pan Fen Feng Feng Chen Duanfang Liao Linxi Chen | 2010 | Acta Biochimica et Biophysica Sinica2010，42，6： | 41 |
| 2 | The EGFR-P38 MAPK axis up-regulates PD-L1 through miR-675-5p and down-regulates HLA-ABC via hexokinase-2 in hepatocellular carcinoma cells显示文摘Background:Immunotherapy has been shown to be a promising strategy against human cancers.A better understanding of the immune regulation in hepatocellular carcinoma(HCC)could help the development of immunotherapy against HCC.The epidermal growth factor receptor(EGFR)signaling is frequently activated in HCC and plays important roles in tumorigenesis.However,its role in HCC immunity is still largely unknown.This study aimed to investigate the impact of EGFR signaling on programmed death-ligand 1(PD-L1)and human leukocyte antigen class-I(HLA-I)expression in HCC cells and its underlying mechanisms.Methods:The expression of phosphorylated EGFR(p-EGFR),PD-L1,and HLAI(HLA-ABC)in HCC specimens was detected by immunohistochemistry,and their correlations were analyzed.PD-L1 and HLA-ABC expression in EGFRactivated HCC cells were detected by quantitative real-time PCR,Western blotting,and flow cytometry,and T cell-mediated lysis was performed to test the immunosuppressive effects of PD-L1 and HLA-ABC alterations in HCC cells.Furthermore,the underlying mechanisms of EGFR activation-induced PD-L1 up-regulation and HLA-ABC down-regulation were explored by animal experiments,luciferase reporter assay,and gene gain-and loss-of-function studies.Results:p-EGFR was positively correlated with PD-L1 and negatively correlated with HLA-ABC expression in HCCs.EGFR activation by its ligand EGF up-regulated PD-L1 and down-regulated HLA-ABC in HCC cells,which was functionally important and could be abolished by the EGFR inhibitor,gefitinib,both in vitro and in vivo.Mechanistically,enhanced P38 mitogenactivated protein kinase(MAPK)activation down-regulated microRNA-675-5p(miR-675-5p)and up-regulated glycolysis-related enzyme hexokinase 2(HK2);miR-675-5p down-regulation enhanced the stability of PD-L1 mRNA probably via the 3’-untranslated region(3’-UTR)of PD-L1 and thereby caused PD-L1 accumulation,and HK2 up-regulation enhanced aerobic glycolysis and mediated a decrease in HLA-ABC.Conclusions:The EGFR-P38 MAPK axis could up-regulate PD-L1 through miR-675-5p and down-regulate HLA-ABC via HK2 in HCC cells.Our study reveals a novel signaling network that may cause immune suppression in HCC and suggests that EGFR signaling can be targeted for HCC immunotherapy. | Zongcai Liu Fen Ning Yanna Cai Huiying Sheng Ruidan Zheng Xi Yin Zhikun Lu Ling Su Xiaodan Chen Chunhua Zeng Haifang Wang Li Liu | 2021 | Cancer Communications2021，41，1： | 5 |
| 3 | Polysaccharides extracted from the roots of Bupleurum chinense DC modulates macrophage functions显示文摘The present study aimed to investigate the effects of polysaccharides extracted from Bupleurum chinense DC(BCPs) on macrophage functions. In the in vivo experiment, 1 m L of 5% sodium thioglycollate was injected into the abdomen of the mice on Day 0 and macrophages were harvested on Day 4. The macrophages were cultured in plates and treated with different concentrations of BCPs and stimulus. Effects of BCPs on macrophage functions were assessed by chemotaxis assay, phagocytosis assay and Enzyme-Linked Immunosorbent Assay(ELISA). Our results showed the enhanced chemotaxis, phagocytosis and secretion of nitric oxide(NO) and inflammatory cytokines by macrophages when treated with BCPs. However, when chemotaxis and phagocytosis were up-regulated by complement components or opsonized particles, BCPs inhibited these effects. Also, the NO production induced by lipopolysaccharides(LPS) was suppressed by BCPs mildly. Moreover, BCPs had an inhibitory effect on the [Ca^(2+)]i elevation of macrophages. These results suggested that BCPs exerted modulatory effects on macrophage functions, which may contribute to developing novel approaches to treating inflammatory diseases. | ZHANG Zhi-De LI Hong WAN Fen SU Xiao-Yu LU Yan CHEN Dao-Feng ZHANG Yun-Yi | 2017 | Chinese Journal of Natural Medicines2017，15，12： | 4 |
| 4 | Quality metrics and outcomes among critically ill patients in China:results of the national clinical quality control indicators for critical care medicine survey 2015-2019显示文摘Background:It is crucial to improve the quality of care provided to ICU patient,therefore a national survey of the medical quality of intensive care units(ICUs)was conducted to analyze adherence to quality metrics and outcomes among critically ill patients in China from 2015 to 2019.Methods:This was an ICU-level study based on a 15-indicator online survey conducted in China.Considering that ICU care quality may vary between secondary and tertiary hospitals,direct standardization was adopted to compare the rates of ICU quality indicators among provinces/regions.Multivariate analysis was performed to identify potential factors for in-hospital mortality and factors related to ventilator-associated pneumonia(VAP),catheter-related bloodstream infections(CRBSIs),and catheter-associated urinary tract infections(CAUTIs).Results:From the survey,the proportions of structural indicators were 1.83%for the number of ICU inpatients relative to the total number of inpatients,1.44%for ICU bed occupancy relative to the total inpatient bed occupancy,and 51.08%for inpatients with Acute Physiology and Chronic Health Evaluation II scores≥15.The proportions of procedural indicators were 74.37%and 76.60%for 3-hour and 6-hour surviving sepsis campaign bundle compliance,respectively,62.93%for microbiology detection,58.24%for deep vein thrombosis prophylaxis,1.49%for unplanned endotracheal extubations,1.99%for extubated inpatients reintubated within 48 hours,6.38%for unplanned transfer to the ICU,and 1.20%for 48-hour ICU readmission.The proportions of outcome indicators were 1.28‰for VAP,3.06‰for CRBSI,3.65‰for CAUTI,and 10.19%for in-hospital mortality.Although the indicators varied greatly across provinces and regions,the treatment level of ICUs in China has been stable and improved based on various quality control indicators in the past 5 years.The overall mortality rate has dropped from 10.19%to approximately 8%.Conclusions:The quality indicators of medical care in China’s ICUs are heterogeneous,which is reflected in geographic disparities and grades of hospitals.This study is of great significance for improving the homogeneity of ICUs in China. | Xi Rui Fen Dong Xudong Ma Longxiang Su Guangliang Shan Yanhong Guo Yun Long Dawei Liu Xiang Zhou | 2022 | Chinese Medical Journal2022，，9： | 4 |
| 5 | Transboundary eco-security and its regulation system in the Longitudinal Range-Gorge Region显示文摘这份报纸讨论了 transboundary eco 安全问题,他们的分发模式,特征和驱动力,和在纵的范围峡区域(LRGR ) 的规定系统。除了沙灰尘暴风雨,主要 transboundary eco 安全问题能在 LRGR 沿着中国的陆上的边阶区域被发现。直接驱动力包括了串联水力发出的电力水坝,高速公路和国际航行开发的构造,斜坡陆地耕作和矿物质探索。Transboundary eco 安全问题沿着国际水路在陆上的区域,和下游的国家影响附近的区域。在邻国的生态的变化在 LRGR 在国内方面上有效果,例如种类侵略。综合措施应该被考虑,包括边阶 eco 保护工程和 transboundary 自然保护区的构造,生态的阀值安装,种类侵略控制,监视平台的 eco 安全的建立,组成生态的赔偿机制和法律(规则) 并且机构,和能力大楼。在 LRGR 处理 transboundary 问题的最重要的任务是国际性地形成参与合作机制,为 transboundary eco 安全建立资金,实现生态的赔偿策略,并且开发信息交换站台和警告系统。 | HE DaMing LIU Jiang HU JinMing FEN Yan GAN Su | 2007 | Chinese Science Bulletin2007，52，A02： | 4 |
| 6 | Comparative strategies for stem cell biodistribution in a preclinical study显示文摘Stem cell therapy represents the potential alternative effective strategy for some diseases that lack effective treatment currently.Correspondingly,it is crucial to establish high-sensitive and reliable quanti?cation assay for tracing exogenous cell migration.In the present study,we?rst used both bioluminescence imaging(BLI)indirect labeling(human norepinephrine transporter-luciferase reporter system)and^89zirconium(^89Zr)-hNSCs direct labeling combined with positron emission tomography/computer tomography(PET/CT)system for tracking human neural stem cells(hNSCs)migration into the brain via nasal administration in preclinical study.But the above two methods failed to give the biodistribution pro?le due to their low sensitivity.Considering its superior sensitivity and absolute quantitation capability,we developed and validated the droplet digital PCR(ddPCR)targeting species-speci?c gene in frozen and paraf?n sections,slices,and whole blood with the sensitivity of 100–200 hNSCs.Accurate and high throughput quanti?cation could be performed using ddPCR with the coef?cient of variation(CVs)of lower quality control(LQC)below 30%.In combination with immunohistochemistry and ddPCR,we con?rmed the migration of hNSCs into the brain via nasal administration,which supported the ef?cacy of hNSCs in MPTP-treated mice,an animal model of Parkinson’s disease.In conclusion,the present study is the?rst to report the application of ddPCR in the pharmacokinetics pro?le description of tracking of hNSCs in preclinical studies. | Fang Wang Zhe Wang Fen Wang Kelly Dong Jing Zhang Yun-juan Sun Chun-feng Liu Mei-jie Xing Xue Cheng Su Wei Jia-wei Zheng Xiong-fei Zhao Xiao-ming Wang Jie Fu Hai-feng Song | 2020 | Acta Pharmacologica Sinica2020，41，4： | 2 |
| 7 | Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population:Findings from the China Cardiometabolic Disease and Cancer Cohort(4C)Study显示文摘Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women. | ZHU Yuan Yue ZHENG Rui Zhi WANG Gui Xia CHEN Li SHI Li Xin SU Qing XU Min XU Yu CHEN Yu Hong YU Xue Feng YAN Li WANG Tian Ge ZHAO Zhi Yun QIN Gui Jun WAN Qin CHEN Gang GAO Zheng Nan SHEN Fei Xia LUO Zuo Jie QIN Ying Fen HUO Ya Nan LI Qiang YE Zhen ZHANG Yin Fei LIU Chao WANG You Min WU Sheng Li YANG Tao DENG Hua Cong ZHAO Jia Jun CHEN Lu Lu MU Yi Ming TANG Xu Lei HU Ru Ying WANG Wei Qing NING Guang LI Mian LU Jie Li BI Yu Fang | 2021 | Biomedical and Environmental Sciences2021，34，1： | 2 |
| 8 | Characteristics of Viral Shedding in Respiratory Samples and Specific Antibodies Production in 564 COVID-19 Patients显示文摘Positive nucleic acid(NA)results have been found in recovered and discharged COVID-19 patients,but the proportion is unclear.This study was designed to analyze the recurrent positive rate of NA results after consecutively negative results,and the relationship between the specific antibody production and positive NA rate.According to Strengthening the Reporting of Observational Studies in Epidemiology guidelines,data of inpatients in Sino-French New City Branch of Tongji Hospital between Jan.28 and Mar.6,2020 were collected.A total of 564 COVID-19 patients over 14 years old who received the examinations of NA and antibodies against SARS-CoV-2 were included.Days of viral shedding and specific antibodies were recorded and assessed.Among NA tests in respiratory samples(throat swabs,nasopharyngeal swabs,sputum and flushing fluid in alveoli),the patients with all-negative NA results accounted for 17.20%,those with single-positive results for 46.63%,and those with multiple-positive results for 36.17%respectively.Besides,the recurrent positive NA results after consecutively negative results appeared in 66 patients(11.70%).For multiple-positive patients,median viral shedding duration was 20 days(range:1 to 57 days).Of the 205 patients who received 2 or more antibody tests,141(68.78%)had decreased IgG and IgM concentrations.IgM decreased to normal range in 24 patients,with a median of 44 days from symptom onset.Viral shedding duration was not significantly correlated with gender,age,disease severity,changes in pulmonary imaging,and antibody concentration.It is concluded that antibody level and antibody change had no significant correlation with the positive rate of NA tests and the conversion rate after continuous negative NA tests.In order to reduce the recurrent positive proportion after discharge,3 or more consecutive negative NA test results with test interval more than 24 h every time are suggested for the discharge or release from quarantine. | Jing GONG Hui DONG Ding-kun WANG Fu-er LU Zhao-yi HUANG Ke FANG Wen-ya HUANG Fen YUAN Xing CHEN Qing-song XIA Le-yi MA Fan WU Hao SU Min-min GONG Yue-heng TANG Ke-xin NIE Zhi WANG Sheng-hao TU Ming-min ZHANG Jing-bin LI | 2021 | Current Medical Science2021，41，1： | 1 |
| 9 | Cytokine IL?6 is required in Citrobacter rodentium infection?inducedintestinal Th17 responses and promotes IL?22 expression in inflammatory boweldisease显示文摘 | Lei Li Qing?Guo Shi Fang Lin Yu?Guang Liang Li?Jian Sun Jin?Song Mu Yong?Gang Wang Hai?Bin Su Biao Xu Cheng?Cheng Ji Hui?Huang Huang Ke Li Hui?Fen Wang | 2014 | Molecular Medicine Reports2014，，3： | 1 |
| 10 | A fuzzy production rule expert system显示文摘 | Zhang Yue Liang fengchi Su fen | 1991 | Fuzzy Sets and Systems1991，44，： | 1 |
| 11 | AN ALTERNATE METHOD TO PREVENT SAND ABRASIVE EROSION IN PIPELINES FOR TRANSPORTING HIGH-SPEED NATURAL GAS显示文摘This paper presents an alternate method to remove the sand carried by natural gas in the upstream pipelines therefore preventing sand abrasive erosion in pipelines used in transporting high speed natural gas. Conventionally, most experts pay much attention to improving the anti erosion characteristics of the pipeline materials to solve the problem of serious abrasive erosion, but without significant success. Based on the theory of multiphase flow and analysis of the characteristics of sandy jets, a new equipment named 'Sand Catcher' is introduced in this article. Experimental results show that the Sand Catcher effectively removes most of the sand in the natural gas and significantly reduces the abrasive erosion of the pipelines. The Sand Catcher can be widely applied in practice in the near future. | Li Hong bo, WU Chao, Li Hua, Zhang Ting, Guo Su fen State Key Hydraulics Laboratory of High Speed Flows, Sichuan University, Chengdu 610065, China | 2002 | Journal of Hydrodynamics2002，14，3： | 1 |
| 12 | Activation and Clinical Significance of p38 MAPK Signaling Pathway in Patients With Severe Trauma显示文摘 | Yi Xin Wang Xin Yun Xu Wen Li Su Qiang Wang Wen Xian Zhu Fen Chen Ge Jin Yu Jian Liu Yi Dong Li Yan Ping Sun Wen Chao Gao Can Ping Ruan | 2010 | Journal of Surgical Research2010，，1： | 1 |
| 13 | The efcacyof complex decongestive physiotherapy (CDP) andpredictive fac-tors of lymphedema severity and response to CDP inbreast cancer - re-lated lymphedema (BCRL) 显示文摘 | Su - Fen Liao a Shu - Hua Lia Hsin - Ya Huang al | 2013 | The Breast2013，，： | 1 |
| 14 | Mediation of β-endorphin in andrographolide-induced plasma glucose-lowering action in type I diabetes-like animals显示文摘 | Bu Chin Yu Cheng Kuei Chang Chih Fen Su Juei Tang Cheng | 2008 | Naunyn - Schmiedeberg’s Archives of Pharmacology (-)2008，，4： | 1 |
| 15 | Do executive stock option grants have value implications for firm performance 显示文摘 | Lam Swee - Su Chng Bey - fen | 2006 | Review of Quantitative Finance&Accounting2006，26，： | 1 |
| 16 | A specular shape from shading by fast marching method 显示文摘 | WANG Guohui SU Wei GAO Fen | 2011 | Procedia Engineering2011，，24： | 1 |
| 17 | Distribution and pathogenicity of bacteria species carried by Bursaphelenchus xylophilus in China显示文摘 | Zhao Bo Guang Wang Hu Li Han Su Fen | 2003 | Nematology2003，，5： | 1 |
| 18 | Autophagy suppresses tumorigenesis of hepatitis B virus‐associated hepatocellular carcinoma through degradation of microRNA‐224显示文摘 | Sheng‐Hui Lan Shan‐Ying Wu Roberto Zuchini Xi‐Zhang Lin Ih‐Jen Su Ting‐Fen Tsai Yen‐Ju Lin Cheng‐Tao Wu Hsiao‐Sheng Liu | 2014 | Hepatology2014，，2： | 1 |
| 19 | Biocompatibility of modified polyethersulfone membranes by blending an amphiphilic triblock co-polymer of poly(vinyl pyrrolidone)–b-poly(methyl methacrylate)–b-poly(vinyl pyrrolidone)显示文摘 | Fen Ran Shengqiang Nie Weifeng Zhao Jie Li Baihai Su Shudong Sun Changsheng Zhao | 2011 | Acta Biomaterialia2011，，9： | 1 |
| 20 | Preparation and characterization of sol–gel derived ZnTiO 3 nanocrystals显示文摘 | Shu Fen Wang Feng Gu Meng Kai Lü Chun Feng Song Su Wen Liu Dong Xu Duo Rong Yuan | 2003 | Materials Research Bulletin2003，，8： | 1 |
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