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		题名	作者	年代	出处	被引量
	1	Verifying Elimination Programs with a Special Emphasis on Cysticercosis Endpoints and Postelimination Surveillance显示文摘	Sukwan Handali Yudi Pawitan Armando Gonzalez	2012	Journal of Parasitology Research2012，，：	1
	2	Construction and expression of a synthetic gene encoding nonstructural glycoprotein NS1 of dengue 2 virus in Pichia pastoris显示文摘Objectives: To express and characterize NS1 of Indonesian-specific DENV2 virus in Pichia pastoris(P. pastoris).Methods: A codon optimized synthetic gene derived from the DENV-2 NS1 amino acid sequences was synthesized commercially and inserted into the P. pastoris pPICZαA expression vector. The recombinant DENV-2 NS1 protein was purified by Ni-NTA affinity chromatography, and its antigenicity was tested.Results: The recombinant DENV-2 NS I protein was secreted as a protein with a molecular weight of ~45 kDa, and the optimal expression condition was achieved by induction with 2%(v/v) methanol for 72 h. The purified recombinant DENV-2 NS1 protein was able to interact with a monoclonal antibody of NS1 in a commercial rapid test.Conclusions: The resulting recombinant DENV-2 NS1 protein produced in P. pastoris KM71 is a potential candidate for use in the development of a dengue diagnostic kit and vaccine.	Fernita Puspasari Riski Dwimalida Putri Aisyah Raden Roro Rika Damayanti Anita Yuwita Bachti Alisjahbana Sukwan Handali Ihsanawati Dessy Natalia	2017	Asian Pacific Journal of Tropical Biomedicine2017，7，8：	0

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