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| 1 | Pressure balance and imbalance in the optic nerve chamber: The Beijing Intracranial and Intraocular Pressure (iCOP) Study显示文摘To determine the interdependence of intracranial pressure(ICP) and intraocular pressure(IOP) and how it affects optic nerve pressures, eight normal dogs were examined using pressure-sensing probes implanted into the left ventricle, lumbar cistern, optic nerve subarachnoid space in the left eye, and anterior chamber in the left eye. This allowed ICP, lumbar cistern pressure(LCP), optic nerve subarachnoid space pressure(ONSP) and IOP to be simultaneously recorded. After establishing baseline pressure levels, pressure changes that resulted from lowering ICP(via shunting cerebrospinal fluid(CSF) from the ventricle) were recorded. At baseline, all examined pressures were different(ICP>LCP>ONSP), but correlated(P<0.001). As ICP was lowered during CSF shunting, IOP also dropped in a parallel time course so that the trans-lamina cribrosa gradient(TLPG) remained stable(ICP-IOP dependent zone). However, once ICP fell below a critical breakpoint, ICP and IOP became uncoupled and TLPG changed as ICP declined(ICP-IOP independent zone). The optic nerve pressure gradient(ONPG) and trans-optic nerve pressure gradient(TOPG) increased linearly as ICP decreased through both the ICP-IOP dependent and independent zones. We conclude that ICP and IOP are coupled in a specific pressure range, but when ICP drops below a critical point, IOP and ICP become uncoupled and TLPG increases. When ICP drops, a rise in the ONPG and TOPG creates more pressure and reduces CSF flow around the optic nerve. This change may play a role in the development and progression of various ophthalmic and neurological diseases, including glaucoma. | Ruowu Hou Zheng Zhang Diya Yang Huaizhou Wang Weiwei Chen Zhen Li Jinghong Sang Sumeng Liu Yiwen Cao Xiaobin Xie Ruojing Ren Yazhuo Zhang Bernhard A. Sabel Ningli Wang | 2016 | Science China(Life Sciences)2016，59，5： | 11 |
| 2 | Knockdown of long non-coding RNA LCPAT1 inhibits autophagy in lung cancer显示文摘Objective: Long non-coding RNAs(lnc RNAs) are involved in numerous biological processes in lung cancer cells. In our previous studies, we identified a lnc RNA, ENST00000439577, which is highly expressed in lung carcinomas, and termed it lung cancer progression-associated transcript 1(LCPAT1). To characterize the role of LCPAT1 in lung cancer, we conducted the current study.Methods: Expression of LCPAT1 and autophagy-associated markers in tumor tissues and lung cancer cell lines was determined by real-time quantitative polymerase chain reaction(q PCR). Hematoxylin and eosin(HE) staining, q PCR, Western blot, and immunohistochemistry were performed to evaluate xenografted tumor tissues. Autophagy induced by rapamycin was detected by Western blot and immunofluorescence in lung cancer cell lines.Results: Expression of LCPAT1 and microtubule-associated protein 1 light chain 3 beta(LC3B) was positively correlated in lung cancer. Knockdown of LCPAT1 inhibited tumor growth and suppressed cell autophagy in vivo. Moreover, LCPAT1 knockdown in lung cancer cell lines resulted in decreased autophagy-associated gene expression and alleviated the cell autophagy induced by rapamycin.Conclusions: We speculate that LCPAT1 plays a crucial role in regulating autophagy in lung cancer. | Xiao Yu Xiaofei Ye Hongyan Lin Nannan Feng Sumeng Gao Xiaohong Zhang Yu Wang Herbert Yu Xiaobei Deng Biyun Qian | 2018 | Cancer Biology & Medicine2018，15，3： | 5 |
| 3 | Liver Function Derangement in Patients with Severe Fever and Thrombocytopenia Syndrome显示文摘Background and Aims:Patients with severe fever with thrombocytopenia syndrome(SFTS)commonly show liver function impairment.This study aimed to characterize the liver function indices in SFTS patients and investigate their association with mortality.Methods:Clinical information and laboratory results of 459 laboratory-confirmed SFTS patients,including 78 deceased and 381 surviving patients,were retrospectively analyzed.To explore the infectivity of SFTS caused by novel Bunyavirus(SFTSV)in hepatocytes,Huh7 human hepatoma cells were infected with various concentrations of SFTSV in vitro.Results:The proportion of SFTS patients developing liver injury during hospitalization was 73.2%(336/459);the hepatocellular injury was the predominant type.The median time to occurrence of liver injury from disease onset was 8 d.Liver injury in the deceased group occurred earlier than that in the surviving group.Alanine aminotransferase(ALT)level between 2–5 times upper limit of normal(ULN)at 4–6 d and between 5–15 ULN at 7–12 d of disease course were independent predictors of mortality.Alkaline phosphatase(ALP)>2 ULN at 7–9 d and elevated ALP at 10–12 days after disease onset were risk factors for death.ALT and aspartate transaminase(AST)levels were correlated with lymphocyte count and platelet-to-lymphocyte ratio(PLR).Total bilirubin(TB),ALT,AST levels showed positive correlation with viral load.In the in vitro experiment,SFTSV infected and replicated inside Huh7 cells.Conclusions:Liver injury is common in SFTS patients.ALT and ALP were independent predictors of SFTS-related mortality.Frequent monitoring and evaluation of liver function indices are needed for SFTS patients. | Sihong Lu Ling Xu Boyun Liang Hua Wang Tong Wang Tiandan Xiang Sumeng Li Lei Fan Junyuan Li Cheng Peng Xin Zheng | 2022 | Journal of Clinical and Translational Hepatology2022，10，5： | 1 |
| 4 | Causal genetic regulation of DNA replication on immune microenvironment in colorectal tumorigenesis: Evidenced by an integrated approach of trans-omics and GWAS显示文摘The interplay between DNA replication stress and immune microenvironment alterations is known to play a crucial role in colorectal tumorigenesis,but a comprehensive understanding of their association with and relevant biomarkers involved in colorectal tumorigenesis is lacking.To address this gap,we conducted a study aiming to investigate this association and identify relevant biomarkers.We analyzed transcriptomic and proteomic profiles of 904 colorectal tumor tissues and 342 normal tissues to examine pathway enrichment,biological activity,and the immune microenvironment.Additionally,we evaluated genetic effects of single variants and genes on colorectal cancer susceptibility using data from genome-wide association studies(GWASs)involving both East Asian(7062 cases and 195745 controls)and European(24476 cases and 23073 controls)populations.We employed mediation analysis to infer the causal pathway,and applied multiplex immunofluorescence to visualize colocalized biomarkers in colorectal tumors and immune cells.Our findings revealed that both DNA replication activity and the flap structure-specific endonuclease 1(FEN1)gene were significantly enriched in colorectal tumor tissues,compared with normal tissues.Moreover,a genetic variant rs4246215 G>T in FEN1 was associated with a decreased risk of colorectal cancer(odds ratio=0.94,95%confidence interval:0.90–0.97,P_(meta)=4.70×10^(-9)).Importantly,we identified basophils and eosinophils that both exhibited a significantly decreased infiltration in colorectal tumors,and were regulated by rs4246215 through causal pathways involving both FEN1 and DNA replication.In conclusion,this trans-omics incorporating GWAS data provides insights into a plausible pathway connecting DNA replication and immunity,expanding biological knowledge of colorectal tumorigenesis and therapeutic targets. | Sumeng Wang Silu Chen Huiqin Li Shuai Ben Tingyu Zhao Rui Zheng Meilin Wang Dongying Gu Lingxiang Liu | 2024 | The Journal of Biomedical Research2024，38，1： | 0 |
| 5 | Suprachoroidal injection of ketorolac tromethamine does not cause retinal damage显示文摘Rabbit right eyes were injected with 3 or 6mg ketorolac tromethamine into the suprachoroidal space. Electroretinography results demonstrated no abnormal changes in rod cell response, maximum rod cell or cone cell mixing reaction, oscillation potential, cone cell response, waveform, amplitude, and potential of 30 Hz scintillation response in right eyes before injection, and at 1,2, and 4 weeks after injection. There was no difference between left (control) and right eyes. Under light microscopy, the histomorphology of cells in each retinal layer was normal at 4 weeks following 6mg ketorolac tromethamine administration. These results indicate that a single suprachoroidal injection of 3 or 6mg ketorolac tromethamine into rabbits was safe. Suprachoroidal space injection appears to be safe. | Sumeng Liu Wu Liu Yaling Ma Kegao Liu Meizi Wang | 2012 | Neural Regeneration Research2012，7，35： | 0 |
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