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| 1 | A novel m6A reader Prrc2a controls oligodendroglial specification and myelination显示文摘While N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is linked to cell differentiation and tissue development, the biological significance of m6A modification in mammalian glial development remains unknown. Here, we identify a novel m6A reader, Prrc2a (Proline rich coiled-coil 2A), which controls oligodendrocyte specification and myelination. Nestin-Cre-mediated knockout of Prrc2a induces significant hypomyelination, decreased lifespan, as well as locomotive and cognitive defects in a mouse model. Further analyses reveal that Prrc2a is involved in oligodendrocyte progenitor cells (OPCs) proliferation and oligodendrocyte fate determination. Accordingly, oligodendroglial-lineage specific deletion of Prrc2a causes a similar phenotype of Nestin-Cre-mediated deletion. Combining transcriptome-wide RNA-seq, m6A-RIP-seq and Prrc2a RIP-seq analysis, we find that Olig2 is a critical downstream target gene of Prrc2a in oligodendrocyte development. Furthermore, Prrc2a stabilizes Olig2 mRNA through binding to a consensus GGACU motif in the Olig2 CDS (coding sequence) in an m6A-dependent manner. Interestingly, we also find that the m6A demethylase, Fto, erases the m6A modification of Olig2 mRNA and promotes its degradation. Together, our results indicate that Prrc2a plays an important role in oligodendrocyte specification through functioning as a novel m6A reader. These findings suggest a new avenue for the development of therapeutic strategies for hypomyelination-related neurological diseases. | Rong Wu Ang Li Baofa Sun Jian-Guang Sun Jinhua Zhang Ting Zhang Yusheng Chen Yujie Xiao Yuhao Gao Qingyang Zhang Jun Ma Xin Yang Yajin Liao Wei-Yi Lai Xiaolong Qi Shukun Wang Yousheng Shu Hai-Lin Wang Fengchao Wang Yun-Gui Yang Zengqiang Yuan | 2019 | Cell Research2019，29，1： | 32 |
| 2 | Factors Affecting Sensory Quality of Cooked japonica Rice显示文摘The sensory quality of cooked rice is an important factor in determining its market price, as well as consumer acceptance and breeding efforts aimed at improvement of rice grain quality. In this study,the sensory quality and physicochemical properties of three japonica rice varieties harvested in two different growing locations(Xiangshui and Hangzhou of China) were compared to determine the most important factors affecting the sensory quality. All the three varieties had higher scores for overall sensory quality in Xiangshui than in Hangzhou, indicating that the growing location is a key factor in determining the sensory quality of cooked japonica rice. In addition to growing location, variety(genotype) also had an important effect. Longdao 18 scored the highest for overall sensory quality in the two locations, whereas Longdao 30 had the lowest score in Xiangshui, and both Longdao 20 and Longdao 30 had the lowest scores in Hangzhou. Many physicochemical properties, such as apparent amylose content, protein content, thermal properties and free amino acid contents, showed significant differences between the two locations. Correlation analysis showed that apparent amylose content and protein content had contrasting effects on all the sensory attributes. The overall sensory quality was negatively correlated with protein content(r =-0.89, P < 0.01) and positively correlated with gel hardness(r = 0.91, P < 0.01),indicating that the protein content and hardness are important physicochemical properties for predicting the sensory quality of japonica rice. These findings will provide guidance for selection from the diverse genotypes available to develop new varieties with the desired eating and cooking quality. | XU Yanjie YING Yining OUYANG Shuhong DUAN Xiaoliang SUN Hui JIANG Shukun SUN Shichen BAO Jinsong | 2018 | Rice science2018，25，6： | 23 |
| 3 | Enhanced performance of ZnO piezoelectric nanogenerators by using Au‐coated nanowire arrays as top electrode显示文摘 | Dechao Yang Wenbin Song Hui Chen Yapeng Li Zhen Zhang Jin Xu Shukun Zhao Xiaoling Sun Yu Qiu | 2015 | Phys. Status Solidi A2015，，： | 1 |
| 4 | Study on blood cultures and bacteria counts in blood of paratyphoid fever A patients显示文摘 | SHUKUN WANG CONGJIA CHU PEISHI SUN | 2009 | Eur J Clin Mierobiol Infect Dis2009，28，10： | 1 |
| 5 | Oligomerization and Polymerization of Ethylene Initiated by a Novel Ni(Ⅱ)-Based Acetyliminopyridine Complexes as Single-Site Catalysts显示文摘新奇的镍( II )基于 acetyliminopyridine 建筑群 1b , 2b , 3b ( 1 ¨C 3b )从 ligands 1a 被综合, 2a , 3a ( 1-3a )并且[ NiCl2 ( DME )],是为为乙烯 oligomerization 和聚合反应是必要的催化剂的合适的先锋,由 methylaluminoxane (毛)激活。对待 MAO 1 ¨ C 3b 介绍一个活跃催化中心,它可以分别地生产线性 | á - 石蜡和聚乙烯的 oligomerize 和聚合乙烯。被获得的 oligomers 的分子量分布在对 oligomers 的 Schulz-Flory 规则的好同意 > C4。3b-MAO 建筑群的活动是 6.3 ? á 10 (7 ) 在 50 点的 g/(molNi-h )??6 号元素碳的化学符号。oligomers 的活动和分子量分布在催化剂先锋的结构上显示出重要信赖。给词调音:oligomerization;聚合;乙烯;镍(II ) 基于催化剂;| á - | Baojun Zhang Yanji Wang Gang Wang Jun Cao Shukun Sun Lihua Xing Yongcheng Sun Yunguang Han | 2007 | Journal of Natural Gas Chemistry2007，16，1： | 1 |
| 6 | Substituent-Dominated Structure Evolution during Sol-Gel Synthesis: A Comparative Study of Sol-Gel Processing of 3-Glycidoxy- propyltrimethoxysilane and Methacryloxypropyl- trimethoxysilane显示文摘 | Shen Shukun Sun Peipei Li Wei | 2010 | Langmuir2010，26，11： | 1 |
| 7 | Advance in technology for catalytic pyrolysis of naphtha for manu- facture of low-carbon olefins and its techno-economic analysis显示文摘 | Liu Jian Sun Shukun Zhang Yongjun | 2011 | Chemical Industry2011，29，11： | 1 |
| 8 | Amino Acid Catalyzed Bulk-phase Gelation of Organoalkoxysilanes via a Transient Co-operative Self-assembly显示文摘 | Shen Shukun Hu Daodao Sun Peipei | 2009 | The Journal of Physical Chemistry B2009，113，13： | 1 |
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