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| 1 | Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis显示文摘瞄准:显示出是否分子的马达达因在在一条微导管轨道,分子的马达肌浆球蛋白 Va,马达 recruiter 肌浆球蛋白 Va, VIIa-Rab27a/b 交往蛋白质(MyRIP ) ,和泡受体上,一条 F 肌动朊轨道上的 Rab27b 在包含 intramanchette 运输(IMT ) 的人和猴子精子形式期间是在场的。方法:Spermiogenic 房间与妨碍的精子缺乏和正常成年 cynomolgus 猴子(Macacafascieularis ) 从三个人被获得。Immunocytochemical 察觉和反向的抄写聚合酶链反应(RT-PCR ) 蛋白质的分析被执行。样品被轻显微镜分析。结果:用 RT-PCR,我们发现了那达因在,肌浆球蛋白 Va, MyRIP 和 Rab27b 在猴子睾丸被表示。,这些蛋白质对领局部性由免疫荧光出现,特别地在人和猴子精子形式期间。结论:我们推测在首领精子形式期间,填写基于微导管、基于肌动朊的泡的那些蛋白质搬运系统,这实际上是在领的现在并且可能可能涉及 intramanchette 运输。 | Shinichi Hayasaka Yukihiro Terada Kichiya Suzuki Haruo Murakawa Ikuo Tachibana Tadashi Sankai Takashi Murakami Nobuo Yaegashi Kunihiro Okamura | 2008 | Asian Journal of Andrology2008，10，4： | 3 |
| 2 | 体内冷冻的正常及镉处理小鼠睾丸内血清蛋白质的免疫组织化学研究显示文摘目的:了解正常和镉(Cd)处理小鼠睾丸内血清蛋白质渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法:保持正常动力学的血循环下,运用活体内冷冻技术对曲细精管内白蛋白和IgG进行快速冷冻固定。冷冻固定的睾丸行冷冻置换后作石蜡包埋。5μm厚连续切片分别用于抗鼠血清白蛋白和IgG的免疫组化染色和H.E.染色。结果:正常曲细精管,白蛋白免疫反应产物主要定位于管周肌样细胞周围,以及间质细胞之间和毛细血管内,部分免疫反应产物呈拱型出现在曲细精管上皮基底室内,沿精原细胞表面分布。在依据生精细胞排列而区分的曲细精管上皮周期的不同发育时相,拱型免疫反应产物的数量不同。正常小鼠睾丸内IgG免疫反应的定位型式与白蛋白的免疫定位相似。Cd处理后24h,部分曲细精管上皮内空泡形成和管腔增大,白蛋白和IgG免疫反应产物不仅出现在基底室,而且也见于近腔室的支持细胞和生精细胞之间。结论:运用活体内快速冷冻技术结合血清白蛋白和IgG的免疫组化方法,能清楚地揭示正常和Cd处理小鼠曲细精管内血清蛋白质的不同免疫染色型式,反映体内支持细胞间紧密连接结构的变化。 | 廖晓岗 Nobuo TERADA 李子龙 Nobuhiko OHNO Yasuhisa FUJII Shinichi OHNO | 2006 | 生殖与避孕2006，26，3： | 2 |
| 3 | Immunoelectron-microscopic study of Kit-expressing cells in the jejunum of wildtype and Ws/Ws rats显示文摘 | Morio Takeda Ichiro Takayama Nobuo Terada Takeshi Baba Sean M. Ward Shinichi Ohno Masayuki A. Fujino | 2001 | Cell and Tissue Research2001，，1： | 1 |
| 4 | Immunolocalization of protein 4.1B/DAL-1 during neoplastic transformation of mouse and human intestinal epithelium显示文摘 | Nobuhiko Ohno Nobuo Terada Shin-ichi Murata Hisashi Yamakawa Irene F. Newsham Ryohei Katoh Osamu Ohara Shinichi Ohno | 2004 | Histochemistry and Cell Biology2004，，6： | 1 |
| 5 | Comparative study of silk fibroin porous scaffolds derived from salt/water and sucrose/hexafluoroisopropanol in carti- lage formation显示文摘 | Makaya Kurniko Terada Shinichi Ohgo Kosuke | 2009 | J Biosci Bioeng2009，108，1： | 1 |
| 6 | 活体冷冻固定及冷冻置换的小鼠睾丸组织内血清白蛋白和IgG的免疫组织化学定位显示文摘目的了解小鼠睾丸组织内血清白蛋白和IgG渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法运用活体内冷冻固定技术对小鼠睾丸进行快速冷冻固定和冷冻置换,并作石蜡包埋。5μm厚连续切片用于抗鼠血清白蛋白和IgG的免疫组化染色和H.E染色。结果H.E染色切片显示距冷冻组织表面300-400μm深度范围内的精曲小管结构保存完好,无明显冰晶形成所致的人工损伤。血清白蛋白和IgG免疫反应产物主要定位于精曲小管管周肌样细胞周围,以及间质细胞之间和毛细血管内,部分免疫反应产物呈拱型出现在精曲小管上皮内,并严格限定在基底室,沿精原细胞表面分布。拱型免疫反应产物的数量与精曲小管上皮周期的发育时相密切相关。结论活体内快速冷冻固定和冷冻置换结合血清白蛋白和IgG的免疫组化方法,可很好地保存精曲小管组织结构并清楚地揭示支持细胞间紧密连接结构的时相变化。 | 廖晓岗 Nobuo Terada 李子龙 Nobuhiko Ohno Yasuhisa Fujii Shinichi Ohno | 2006 | 中国组织化学与细胞化学杂志2006，15，3： | 0 |
| 7 | Immunohistochemical Study of Serum Proteins in Normal and Cadmium-Treated Mouse Testis by in vivo Cryotechnique显示文摘Objective To investigate time-dependent changes of serum proteins permeability inthe normal and cadmium(Cd)-treated mouse testis, reflecting tight junctional (TJ) barriersof Sertoli cellsMethods The serum proteins, albumin and immunoglobulin-G(IgG), in the seminiferoustubules were firstly immobilized by the “in vivo cryotechnique”, in which the dynamicblood circulation was always kept. The cryofixed testicular tissues were then processedfor the freeze-substitution method, and embedded in the paraffin wax. Serial sectionsof 5 μm thickness were immunostained by anti-mouse albumin or IgG antibody withperoxidase immunostaining, and also stained with hematoxylin-eosine (HE) formorphological observation.Results In normal seminiferous tubules, albumin immunoreaction products werelocalized around peritubular myoid cells and among Leydig cells, as well as in bloodvessels. They were also localized as arch-like patterns around some spermatogonia inbasal compartments. The number of the immunopositive arch structures was differentaccording to developmental stages of the seminiferous cycle, judging from thearrangement of germ cells by HE-staining. The patterns of localization of IgGimmunostaining in normal mouse testis were similar to that of albumin. In 24 h afterCd-treatment, some enlarged spaces and vesicular formation in the seminiferousepithelium were observed on the paraffin sections by HE-staining. The albumin or IgGimmunolocalization was seen not only in the basal compartments, but also in theadluminal compartments between Sertoli cells and germ cells.Conclusion The structural changes of inter-Sertoli TJ barriers in vivo, such as differentimmunostaining patterns of serum proteins between the normal and Cd-treated mouseseminiferous tubules, could be clearly detected by the “in vivo cryotechnique” withalbumin or IgG immunohistochemistry. | Nobuo TERADA Nobuhiko OHNO Yasuhisa FUJII Shinichi OHNO | 2005 | Journal of Reproduction and Contraception2005，16，3： | 0 |
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