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| 1 | In vivo radiometric analysis of glucose uptake and distribution in mouse bone显示文摘Bone formation and remodeling occurs throughout life and requires the sustained activity of osteoblasts and osteoclasts,particularly during periods of rapid bone growth.Despite increasing evidence linking bone cell activity to global energy homeostasis,little is known about the relative energy requirements or substrate utilization of bone cells.In these studies,we measured the uptake and distribution of glucose in the skeleton in vivo using positron-emitting^(18)F-fluorodeoxyglucose([^(18)F]-FDG) and non-invasive,high-resolution positron emission tomography/computed tomography(PET/CT) imaging and ex vivo autoradiography.Assessment of [^(18)F]-FDG uptake demonstrated that relative to other tissues bone accumulated a significant fraction of the total dose of the glucose analog.Skeletal accumulation was greatest in young mice undergoing the rapid bone formation that characterizes early development.PET/CT imaging revealed that [^(18)F]-FDG uptake was greatest in the epiphyseal and metaphyseal regions of long bones,which accords with the increased osteoblast numbers and activity at this skeletal site.Insulin administration significantly increased skeletal accumulation of [^(18)F]-FDG,while uptake was reduced in mice lacking the insulin receptor specifically in osteoblasts or fed a high-fat diet.Our results indicated that the skeleton is a site of significant glucose uptake and that its consumption by bone cells is subject to regulation by insulin and disturbances in whole-body metabolism. | Meredith L Zoch Diane S Abou Thomas L Clemens Daniel LJ Thorek Ryan C Riddle | 2016 | Bone Research2016，4，1： | 9 |
| 2 | Molecularly specific detection of bacterial lipoteichoic acid for diagnosis of prosthetic joint infection of the bone显示文摘Discriminating sterile inflammation from infection, especially in cases of aseptic loosening versus an actual prosthetic joint infection, is challenging and has significant treatment implications. Our goal was to evaluate a novel human monoclonal antibody(mAb) probe directed against the Gram-positive bacterial surface molecule lipoteichoic acid(LTA). Specificity and affinity were assessed in vitro. We then radiolabeled the anti-LTA mAb and evaluated its effectiveness as a diagnostic imaging tool for detecting infection via immuno PET imaging in an in vivo mouse model of prosthetic joint infection(PJI). In vitro and ex vivo binding of the anti-LTA mAb to pathogenic bacteria was measured with Octet, ELISA, and flow cytometry. The in vivo PJI mouse model was assessed using traditional imaging modalities, including positron emission tomography(PET) with [^(18)F]FDG and [^(18)F]Na F as well as X-ray computed tomography(CT), before being evaluated with the zirconium-89-labeled antibody specific for LTA([^(89)Zr]SAC55).The anti-LTA mAb exhibited specific binding in vitro to LTA-expressing bacteria. Results from imaging showed that our model could reliably simulate infection at the surgical site by bioluminescent imaging, conventional PET tracer imaging, and bone morphological changes by CT. One day following injection of both the radiolabeled anti-LTA and isotype control antibodies, the anti-LTA antibody demonstrated significantly greater(P < 0.05) uptake at S. aureus-infected prosthesis sites over either the same antibody at sterile prosthesis sites or of control non-specific antibody at infected prosthesis sites. Taken together, the radiolabeled anti-LTA mAb, [^(89)Zr]SAC55, may serve as a valuable diagnostic molecular imaging probe to help distinguish between sterile inflammation and infection in the setting of PJI. Future studies are needed to determine whether these findings will translate to human PJI. | Julie E.Pickett John M.Thompson Agnieszka Sadowska Christine Tkaczyk Bret R.Sellman Andrea Minola Davide Corti Antonio Lanzavecchia Lloyd S.Miller Daniel LJ Thorek | 2018 | Bone Research2018，6，2： | 3 |
| 3 | In vivo imaging of cancer biomarkers using aetivatable molecular probes显示文摘 | Elias D R Thorek D L J Chen A K | 2008 | Caneer Biomarkers2008，4，6： | 1 |
| 4 | Superparamagnetic iron oxide nanoparticle probes for molecular imaging显示文摘 | Thorek D Chen A Czupryna J | | 0，，1： | 1 |
| 5 | Superparamagnetic iron oxide nanoparticle probes for molecular imaging 显示文摘 | Thorek DL Chen AK Czupryna J | 2006 | Ann Biomed Eng2006，34，1： | 1 |
| 6 | H;adolinium conjugated den drimer nanoclusters as a tUl31or targeted tllagrlcqi, resonance imaging contrast agent显示文摘 | Cheng Z Thorek I3 L Tsourkas A | 2010 | Angew Chem(Int Ed Engl)2010，19，2： | 1 |
| 7 | Radiolabeled Antibodies in Prostate Cancer: A Case Study Showing the Effect of Host Immunity on Antibody Bio-distribution显示文摘 | Oskar Vilhelmsson-Timmermand Elmer Santos Daniel L.J. Thorek Susan Evans-Axelsson Anders Bjartell Hans Lilja Steven M. Larson Sven-Erik Strand Thuy A. Tran David Ulmert | 2014 | Nuclear Medicine and Biology2014，，： | 1 |
| 8 | Clinical Cerenk- ov luminescence imaging of 18F-FDG显示文摘 | Thorek D L J Riedl C C Grimm J | 2014 | Journal of Nuclear Medicine2014，55，1： | 1 |
| 9 | Possibilities in the reconstruction of the human 显示文摘 | Thorek M | 1922 | NY Med J1922，116，： | 1 |
| 10 | Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging显示文摘 | Daniel L. J. Thorek Antony K. Chen Julie Czupryna Andrew Tsourkas | 2006 | Annals of Biomedical Engineering2006，，1： | 1 |
| 11 | Clinical Cerenkov luminescence imaging of F- 18-FDG显示文摘 | Thorek DL Riedl CC Grimm J | 2013 | Journal of Nuclear Medicine2013，55，1： | 1 |
| 12 | Superparamagnetie iron oxide nanopartiele probes for molecular imaging显示文摘 | Thorek DL Chen AK Czupryna J | 2006 | Ann Biomed Eng2006，34，1： | 1 |
| 13 | Non- invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle 显示文摘 | THOREK D L ULMERT D DIOP N F | 2014 | Nat Commun2014，5，5： | 1 |
| 14 | Superparamagnetic iron oxide nanoparticle probes for molecular imaging显示文摘 | Thorek DL Chen AK Czupryna J | 2006 | Ann Biomed Eng2006，34，1： | 1 |
| 15 | Gadolinium-eoniugated den- drimer nanoclnsters as a tumor-targeted TI magnetic resonance imaging contrast agent显示文摘 | Cheng Z Thorek DL Tsourkas A | 2010 | Angewandte Chemie International E- dition2010，49，34： | 1 |
| 16 | Gadolinium-conjugated dendrimer nanoclusters as a tumor-targeted T1 magnetic resonance imaging contrast agent 显示文摘 | Cheng Z Thorek D1 Tsourkas A | 2010 | Angew Chem Int Ed Engl2010，49，： | 1 |
| 17 | 89Zr-Labeled paramagnetic octreotide-liposomes for PET-MR imaging of cancer显示文摘 | Abou D S Thorek D L Ramos N N | 2013 | Pharm Res2013，30，： | 1 |
| 18 | Gadolinium-conjugated den- drimer nanoclusters as a tumor-targeted T1 magnetic resonance imaging contrast agent显示文摘 | Cheng Z Thorek D L Tsourkas A | 2010 | Angcw Chem Int Ed Engl2010，49，2： | 1 |
| 19 | Molecular imaging using nanoparticle quenchers of Cerenkov luminescence 显示文摘 | Thorek D L J Das S Grimm J | 2014 | Small2014，10，18： | 1 |
| 20 | Clinical Cerenkov luminescence imaging of ~gF - FDG显示文摘 | Thorek D L J Riedl C C Grimm J | 2014 | Journal of Nu- clear Medicine2014，55，1： | 1 |
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