|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | PAQR10 and PAQR11 mediate Ras signaling in the Golgi apparatus显示文摘地岬在许多细胞的活动起一个枢轴的作用,并且它的 subcellular 区分提供空间、时间的选择。这里,我们报导由二高度相应的蛋白质 PAQR10 和孕激素和 AdipoQ 受体家庭的 PAQR11 在 Golgi 仪器发信号的地岬的空间规定的一个模式。PAQR10 和 PAQR11 在 Golgi 仪器是只局部性的。PAQR10/PAQR11 的 Overexpression 刺激基础、导致 EGF 的英皇家空军之阶级最低之兵 phosphorylation 并且以一种剂量依赖者方式增加英皇家空军之阶级最低之兵目标基因的表示。PAQR10/PAQR11 的 Overexpression 显著地提高 HRas, NRas 和 KRas4A 的 Golgi 本地化,然而并非 KRas4B。PAQR10 和 PAQR11 罐头也与 HRas, NRas 和 KRas4A 交往,然而并非 KRas4B。在由 PAQR10/PAQR11 的 overexpression 的 Golgi 仪器的增加的地岬蛋白质处于一个活跃状态。一致地, PAQR10 和 PAQR11 还原剂击倒在 Golgi 仪器的刺激 EGF 的英皇家空军之阶级最低之兵 phosphorylation 和地岬激活。有趣地, PAQR10 和 PAQR11 能与 RasGRP1,地岬的 guanine 核苷酸交换蛋白质,和 RasGRP1 的增加 Golgi 本地化交往。RasGRP1 的 C1 领域为和 PAQR10/PAQR11 的 RasGRP1 的相互作用必要、足够。由 overexpressed PAQR10/PAQR11 的英皇家空军之阶级最低之兵 phosphorylation 的模拟被 RasGRP1 的 downregulation 废除。而且, PC12 房间的区别被 PAQR10/PAQR11 的 overexpression 显著地提高。一起,这研究揭开由 PAQR10 和 PAQR11 在 Golgi 仪器发信号的地岬的空间规定的一个新范例。 | Ting Jin Qiurong Ding Heng Huang Daqian Xu Yuhui Jiang Ben Zhou Zhenghu Li Xiaomeng Jiang Jing He Weizhong Liu Yixuan Zhang Yi Pan Zhenzhen Wang Walter G Thomas Yan Chen | 2012 | Cell Research2012，22，4： | 6 |
| 3 | Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows显示文摘Known as the‘third polar region’,the Qinghai-Tibet Plateau represents one of the harshest highland environments in the world and yet a number of organisms thrive there.Previous studies of birds,animals and humans have focused on well-differentiated populations in later stages of phenotypic divergence.The adaptive processes during the initial phase of highland adaptation remain poorly understood.We studied a human commensal,the Eurasian Tree Sparrow,which has followed human agriculture to the Qinghai-Tibet Plateau.Despite strong phenotypic differentiation at multiple levels,in particular in muscle-related phenotypes,highland and lowland populations show shallow genomic divergence and the colonization event occurred within the past few thousand years.In a one-month acclimation experiment investigating phenotypic plasticity,we exposed adult lowland tree sparrows to a hypoxic environment and did not observe muscle changes.Through population genetic analyses,we identified a signature of polygenic adaptation,whereby shifts in allele frequencies are spread across multiple loci,many of which are associated with muscle-related processes.Our results reveal a case of positive selection in which polygenic adaptation appears to drive rapid phenotypic evolution,shedding light on early stages of adaptive evolution to a novel environment. | Yanhua Qu Chunhai Chen Ying Xiong Huishang She Yong E Zhang Yalin Cheng Shane DuBay Dongming Li Per G P Ericson Yan Hao Hongyuan Wang Hongfeng Zhao Gang Song Hailin Zhang Ting Yang Chi Zhang Liping Liang Tianyu Wu Jinyang Zhao Qiang Gao Weiwei Zhai Fumin Lei | 2020 | National Science Review2020，7，1： | 2 |
| 4 | Off-line high-performance liquid chromatography and solid-phase extraction clean-up for confirmation of pesticide residues in fresh produce by gas chromatography-mass spectrometry显示文摘 | TING K C TAMASHIRO G S | 1996 | Journal of Chromatography1996，754，： | 1 |
| 5 | Uptake of cadmium and zinc by the alga Chlorella vulgaris (II):Multi-ion situation显示文摘 | TING Y P PRINCE I G LAWSON F | 1991 | Biotechnol Bioeng1991，37，： | 1 |
| 6 | Importance of acidic,proline/setine/threonine-rich,and GTP-binding regions in the major histocompatibility complex class Ⅱ transac tivator generation of transdominant negative mutants显示文摘 | LiG G Ting JP | 1997 | Proe Nail Acad Sci USA1997，94，6： | 1 |
| 7 | Soil organic matter characteristic as affected by tillage management显示文摘 | Ting G Novak J M Amarasiriwardena D | 2002 | Soil Science Society of America Journal2002，66，： | 1 |
| 8 | The anisotropic elastic solid with an elliptic hole or rigid inclusion显示文摘 | TING T C T YAN G | 1991 | International Journal of Solids and Structures1991，27，15： | 1 |
| 9 | Genetic association between IL-17Fgene polymorphisms and the pathogenesis of Graves'Disease in theHan Chinese population 显示文摘 | Ting G Ya H Wei Z | 2013 | Gene2013，512，2： | 1 |
| 10 | Microparticles of poly ( DL-lactic-co-glycolic acid )/poly ( ethylene glycol ) blends for controlled drug delivery 显示文摘 | Robert L Cleek Karen C Ting Suzanne G Eskin | 1997 | J Controlled Release1997，48，： | 1 |
| 11 | Accurate measure- ment of stable isotopes of lithium by inductively coupled plasma mass spectrometry 显示文摘 | Sun X F Ting B T G Zeisel S H | 1987 | Analyst1987，，112： | 1 |
| 12 | Short dsRNA Induces Transcriptional Gene Silencing in Human Cancer Cells in the Absence of DNA Methylation显示文摘 | Ting A H Sehuebel K E Herman J G | 2005 | Nat Genet2005，37，8： | 1 |
| 13 | Synthesis and microwave electromagnetic characteristics of bamboo char- coallpolyaniline composites in 2-40GHz 显示文摘 | WU K H TING T H WANG G P et ol | 2008 | Synth Met2008，158，1718： | 1 |
| 14 | Cyanobacterial photosynthesis in the oceans: The origins and significance of divergent light-harvesting strategies显示文摘 | Ting C S Rocap G King J | 2002 | Trends in Microbiology2002，10，3： | 1 |
| 15 | Simultaneous analysis of microbial identity and function using NanoSIMS显示文摘 | TianlunLi Ting‐DiWu LaurentMazéas LaurentToffin Jean‐LucGuerquin‐Kern GérardLeblon ThéodoreBouchez | 2008 | Environmental Microbiology2008，，3： | 1 |
| 16 | Shortdoubh-stranded RNA induces transcriptional genc silencing in human cancer cells in he absence of DNA methylation显示文摘 | Ting A H Schuebel K E Herman J G | 2005 | Nature Genet2005，37，8： | 1 |
| 17 | Hydroxyapatite coating on Ti6A14V alloy by a sol-gel melhod显示文摘 | WANG D G CHESN C Z TING H | 2008 | Materials Science: Materials in Medicine2008，19，6： | 1 |
| 18 | Soil organic matter characteristic as affected by tillage management显示文摘 | TING G NOVAK J M AMAGASAKI W D | 2002 | Soil Science Society of America Journal2002，66，2： | 1 |
| 19 | Cleaning up after ICH:the role of Nrf2 in modulating microglia function and hematoma clearance显示文摘 | Zhao X Sun G Ting SM | 2015 | J Neurochem2015，133，： | 1 |
| 20 | Apico-basal forces exerted by apoptotic cells drive epithelium folding 显示文摘 | MONIER B GEq'TINGS M GAY G | 2015 | Nat2015，518，7538： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员