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| 1 | A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp.pekinensis)Selection显示文摘Chinese cabbage is the most consumed leafy crop in East Asian countries.However,premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic distribution.In the past 40years,spring Chinese cabbage with strong winterness has been selected to meet the market demand.Here,we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ecotypes.In-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern China.We identified 23 genomic loci that underwent intensive selection,and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1(BrVIN3.1)and FLOWER LOCUS C 1(BrFLC1)is a determinant genetic source of variation during selection.Moreover,we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVlN3.1 promoter significantly contributes to bolting-time variation in Chinese cabbage.Collectively, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant Varieties by molecular-marker-assisted selection,transgenic or gene editingapproaches. | Tongbing Su Weihong Wang Peirong Li Bin Zhang Pan Li Xiaoyun Xin Honghe Sun Yangjun Yu Deshuang Zhang Xiuyun Zhao Changlong Wen Gang Zhou Yuntong Wang Hongkun Zheng Shuancang Yu Fenglan Zhang | 2018 | Molecular Plant2018，11，11： | 6 |
| 2 | Ethylene signaling is required for the acceleration of cell death induced by the activation of AtMEK5 in Arabidopsis显示文摘激活 Mitogen 的蛋白质家族 ases (MAPK ) 涉及到许多刺激的植物生长,开发和回答的规定。在有条件的 gain-of-function 转基因的系统, AtMEK5 的激活, MAPK kinase,能接着激活内长的 AtMAPK3, AtMAPK6,和罐头在乙烯生产导致惹人注目的增加并且在 Arabidopsis 导致象房间死亡一样的过分敏感的反应(HR ) 。然而,在调整这象 HR 一样房间死亡的增加的乙烯生产的角色仍然保持未知。用表示 AtMEK5 (DD ) 的 Arabidopsis 转基因的植物,在一个类固醇可诱导的倡导者的控制下面的 AtMEK5 的活跃异种,我们测试了乙烯的贡献到房间死亡。我们发现乙烯生合成发生在房间死亡前。房间死亡被用 ACC-synthases, ACC-oxidases 或乙烯受体的禁止者禁止导致 AtMEK5 的乙烯生产推迟。在异种 AtMEK5 ( DD ) /etr1-1 和 AtMEK5 ( DD ) /ein2-1 ,哪个在敏感证明生产与在 AtMEK5 ( DD )看见的那些一样到乙烯, AtMEK5 ( DD )蛋白质的表示, AtMAPK3 和 AtMAPK6 的活动,和乙烯转基因的植物,但是房间死亡也被推迟。这些数据建议乙烯发信号感觉被要求加速被 AtMEK5 激活导致的房间死亡。 | Hongxia Liu Ying Wang Juan Xu Tongbing Su Guoqin Liu Dongtao Ren | 2008 | Cell Research2008，18，3： | 5 |
| 3 | Identification of long noncoding RNAs involved in resistance to downy mildew in Chinese cabbage显示文摘Brassica downy mildew,a severe disease caused by Hyaloperonospora brassicae,can cause enormous economic losses in Chinese cabbage(Brassica rapa L.ssp.pekinensis)production.Although some research has been reported recently concerning the underlying resistance to this disease,no studies have identified or characterized long noncoding RNAs involved in this defense response.In this study,using high-throughput RNA sequencing,we analyzed the disease-responding mRNAs and long noncoding RNAs in two resistant lines(T12–19 and 12–85)and one susceptible line(91–112).Clustering and Gene Ontology analysis of differentially expressed genes(DEGs)showed that more DEGs were involved in the defense response in the two resistant lines than in the susceptible line.Different expression patterns and proposed functions of differentially expressed long noncoding RNAs among T12–19,12–85,and 91–112 indicated that each has a distinct disease response mechanism.There were significantly more cis-and trans-functional long noncoding RNAs in the resistant lines than in the susceptible line,and the genes regulated by these RNAs mostly participated in the disease defense response.Furthermore,we identified a candidate resistance-related long noncoding RNA,MSTRG.19915,which is a long noncoding natural antisense transcript of a MAPK gene,BrMAPK15.Via an agroinfiltration-mediated transient overexpression system and virus-induced gene silencing technology,BrMAPK15 was indicated to have a greater ability to defend against pathogens.MSTRG.19915-silenced seedlings showed enhanced resistance to downy mildew,probably because of the upregulated expression of BrMAPK15.This research identified and characterized long noncoding RNAs involved in resistance to downy mildew,laying a foundation for future in-depth studies of disease resistance mechanisms in Chinese cabbage. | Bin Zhang Tongbing Su Peirong Li Xiaoyun Xin Yunyun Cao Weihong Wang Xiuyun Zhao Deshuang Zhang Yangjun Yu Dayong Li Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 4 | Physico-chemical factors and bacteria in fish ponds显示文摘 | X Jun F Xiuzheng Y Tongbing | 2000 | Naga The ICLARM Quarterly2000，23，4： | 1 |
| 5 | Genome-wide analysis of changes in miRNA and target gene expression reveals key roles in heterosis for chinese cabbage biomass显示文摘Heterosis is a complex phenomenon in which hybrids show better phenotypic characteristics than their parents do.Chinese cabbage(Brassica rapa L.spp.pekinensis)is a popular leafy crop species,hybrids of which are widely used in commercial production;however,the molecular basis of heterosis for biomass of Chinese cabbage is poorly understood.We characterized heterosis in a Chinese cabbage hybrid cultivar and its parental lines from the seedling stage to the heading stage;marked heterosis of leaf weight and biomass yield were observed.Small RNA sequencing revealed 63 and 50 differentially expressed microRNAs(DEMs)at the seedling and early-heading stages,respectively.The expression levels ofthe majority of miRNA clusters in the hybrid were lower than the mid-parent values(MPVs).Using degradome sequencing,we identi fied 1,819 miRNA target genes.Gene ontology(GO)analyses demonstrated that the target genes ofthe MPV-DEMs and low parental expression level dominance(ELD)miRNAs were signi ficantly enriched in leaf morphogenesis,leaf development,and leaf shaping.Transcriptome analysis revealed that the expression levels of photosynthesis and chlorophyll synthesis-related MPV-DEGs(differentially expressed genes)were signi ficantly different in the F_(1) hybrid compared to the parental lines,resulting in increased photosynthesis capacity and chlorophyll content in the former.Furthermore,expression of genes known to regulate leaf development was also observed at the seedling stage.Arabidopsis plants overexpressing BrGRF4.2 and bra-miR396 presented increased and decreased leaf sizes,respectively.These results provide new insight into the regulation of target genes and miRNA expression patterns in leaf size and heterosis for biomass of B.rapa. | Peirong Li Tongbing Su Deshuang Zhang Weihong Wang Xiaoyun Xin Yangjun Yu Xiuyun Zhao Shuancang Yu Fenglan Zhang | 2021 | Horticulture Research2021，8，1： | 1 |
| 6 | Complex color centers in ultraviolet-light irradiated single PbWO4 crystal 显示文摘 | LI Wen-sheng TANG Tongb FENG Xi-qi | 2000 | Journal of Applied Physics2000，87，11： | 1 |
| 7 | Existence and design of biorthogonal matrix-valued wavelets显示文摘 | Lihong Cui Bolan Zhai Tongbing Zhang | 2008 | Nonlinear Analysis: Real World Applications2008，，5： | 1 |
| 8 | Synthesis of high solid content waterborne polyurethanes with controllable bimodal particle size distribution显示文摘 | Peng Shao Jun Jin Yong Sun Tongbing | 2014 | Journal of Applied Polymer Science2014，131，40： | 1 |
| 9 | Simultaneous removal of NOx and SO2 from coal boiler title gases by DC corona discharge ammonia radical shower systems: pilot plant tests显示文摘 | Chang J S Urashima K Tongb Y X | 2003 | Journal of Electrostatics2003，57，： | 1 |
| 10 | Simultaneous removal of NOx and SO2 from coal boiler flue gases by DC corona discharge ammonia radical shower systems: Pilot plant tests 显示文摘 | Chang J S Urashima K Tongb Y X | 2003 | Journal of Electrostatics2003，57，34： | 1 |
| 11 | DNAmethylationandagingDNA显示文摘 | TongB(童备) ZhangZY(张宗玉) | | 生命的化学0，，： | 1 |
| 12 | Self-assembly of homopolymer of PAA-NH_4显示文摘Self-assembly of homo-polymers has rarely been reported. Herein, PAA-NH_4 assemblies varying from nanospheres to large particles and yolk-shell vesicles were obtained by adding different amount of HCI solution into the dispersion of PAA-NH_4 in ethanol. The changes of zeta potentials, pH value and microstructure of the PAA-NH_4 assemblies were characterized, and the influences of molecular weight and different alcohols on the assembly morphologies were studied. A possible assembly mechanism based on the solubility and electrostatic interaction was proposed. Our study offered an interesting example of homo-polymer assembly and may extend the practical application due to the simple polymers used. | Tongbing Sun Xiaoli Yang Caizhen Zhu Ning Zhao Haixia Dong Jian Xu | 2019 | Chinese Chemical Letters2019，30，2： | 0 |
| 13 | CCL26 is upregulated by nab-paclitaxel in pancreatic cancer-associated fibroblasts and promotes PDAC invasiveness through activation of the PI3K/AKT/mTOR pathway显示文摘Recently,the combined use of FOLFIRINOX(leucovorin and fluorouracil plus irinotecan and oxaliplatin)and gemcitabine plus nab-paclitaxel has significantly improved the prognosis of patients with pancreatic cancer.However,there is still a high proportion of patients who develop metastatic pancreatic cancer in the course of chemotherapy or within a short period after chemotherapy.Previous reports have shown that chemotherapy-driven cytokine storms or the direct effects of certain chemotherapeutics on stromal and/or immune cells collectively change the microenvironment of the primary tumor,thus indirectly promoting metastasis.However,the mechanism underlying chemotherapy-induced metastasis in the course of chemotherapy,and afterwards,remains elusive in pancreatic cancer.In the present study,we aimed to determine the expression of CCL26 in the pancreatic cancer-associated fibroblasts(CAFs)after nab-paclitaxel treatment and to explore the role of CCL26 in the pancreatic adenocarcinoma(PDAC)invasion.Our results showed that nab-paclitaxel increased CCL26 mRNA and protein expression levels in a dose-and time-dependent manner.Subsequently,PDAC cell lines were treated with recombinant CCL26 for 48 h.The transwell migration assay showed that recombinant CCL26 enhanced the invasion of PDAC cells.Western blot analysis showed that the protein expression levels of phospho-(p-)PI3K,p-AKT,and p-mTOR were increased by CCL26 in PDAC cells.CCL26 expressions in 95 PDAC tissues and adjacent normal tissues were evaluated using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry.CCL26 was found to be overexpressed in PDAC samples,and upregulated CCL26 expression was significantly associated with advanced perineural invasion,lymph node metastasis,and poor differentiation.In summary,our results showed that nab-paclitaxel increased the expression of CCL26 in CAFs,and CCL26 enhanced the invasive potential of pancreatic cancer cells by activating the PI3K/AKT/mTOR axis.Thus,CCL26 may be a potential prognostic biomarker for pancreatic cancer. | Xuemin Chen Yong An Yue Zhang Dong Xu Tongbing Chen Yue Yang Weibo Chen Di Wu Xiaoying Zhang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 0 |
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