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您的检索式：作者名="Tonghang Guo"
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| 1 | Meiotic Chromosome Behavior in a Human Male t(8;15)Carrier显示文摘Reciprocal translocation is one of the most common structural chromosomal rearrangements in human beings;it is widely recognized to be associated with male infertility.This association is mainly based on the abnormal chromosome behavior of the translocated chromosomes and sex chromosomes during meiosis prophase I in reciprocal translocation carriers.However,the underlying mechanisms are not completely known.Here we report a reciprocal translocation carrier of t(8;15),who is oligozoospermic due to apoptosis of primary spermatocytes and to premature germ cell desquamation from seminiferous tubules.Further analysis showed abnormal synapsis and recombination frequency in this patient,indicating a connection between chromosome behavior and apoptosis of primary spermatocytes.We also compared these observations with recently reported findings on spermatogenesis defects in reciprocal translocation carriers,and discuss the possible mechanisms underlying bom common and unique phenotypes of reciprocal translocations involving different chromosomes with the aim of further understanding the regulation of human spermatogenesis. | Hanwei Jiang Liu Wang Yingxia Cui Zhipeng Xu Tonghang Guo Dongkai Cheng Peng Xu Wen Yu Qinghua Shi | 2014 | Journal of Genetics and Genomics2014，41，3： | 2 |
| 2 | Experimental cloning of embryos through human-rabbit inter-species nuclear transfer显示文摘Therapeutic cloning,which is based on human somatic cell nuclear transfer,is one of our major research objectives.Though inter-species nuclear transfer has been introduced to construct human somatic cell cloned embryos,the effects of type,passage,and preparation method of donor cells on embryo development remain unclear.In our experiment,cloned embryos were reconstructed with different passage and preparation methods of ossocartilaginous cell,skin fibroblast,and cumulus cells.The cumulus cell embryos showed significantly higher development rates than the other two(P<0.05).The development rate of embryos reconstruct-ed with skin fibroblasts of different passage number and somatic cells of different chilling durations showed no significant difference.Also,fluorescence in situ hybridization(FISH)was conducted to detect nuclear derivation of the embryos.The result showed that the nuclei of the inter-species cloned embryo cells came from human.We conclude that(1)cloned embryos can be constructed through human-rabbit interspecies nuclear transfer;(2)different kinds of somatic cells result in different efficiency of nuclear transfer,while in vitro passage of the donor does not influence embryo development;(3)refrigeration is a convenient and efficient donor cell preparation method.Finally,it is feasible to detect DNA genotype through FISH. | JI Jingjuan GUO Tonghang TONG Xianhong LUO Lihua ZHOU Guixiang FU Yingyun LIU Yusheng | 2007 | Frontiers in Biology2007，2，1： | 0 |
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