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| 1 | Changes in Antioxidant Enzyme Activities and Malondialdehyde(MDA) Content of Rice with Blast Resistance Induced by Ag-antibiotic 702显示文摘The paper was to explore the effect of rice blast resistance induced by Ag-antibiotic 702 on antioxidant enzyme activities and malondialdehyde( MDA)content. At three-leaf and one-heart stage of Luliangyou 996,15 μg/mL Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. The antioxidant enzyme( SOD,POD,PPO,CAT and PAL) activities and malondialdehyde( MDA) content in rice leaves were determined every 24 h within 168 h post spraying. Compared with CK,the MDA content and activities of the five antioxidant enzymes increased in Agantibiotic 702 treatment,Validamycin treatment and M. grisea inoculation treatment. The MDA content and SOD,CAT and POD activities in Ag-antibiotic 702 treatment were significantly higher than those in M. grisea inoculation treatment in early stage,and the APX and LOX activities increased more remarkably and rapidly. The induction effect of Ag-antibiotic 702 could reach or exceed that of Validamycin treatment. Ag-antibiotic 702 enhanced the resistance to rice blast because MDA content and antioxidant enzymes activities were improved in rice leaves. Therefore,the increase of rice blast resistance induced by Ag-antibiotic 702 in rice was positively correlated with the increase of MDA content and antioxidant enzymes activities in rice leaves. | Hu Neng Tu Xiaorong Li Kuntai Liu Yongkang Guo Amin Tu Guoquan Huang Lin | 2017 | Plant Diseases and Pests2017，8，2： | 5 |
| 2 | Effects of Combination of Straw Returning and a Microbial Agent on Microorganisms and Enzyme Activity in Rhizosphere Soil and Yield of Late Rice显示文摘By using red soil and late rice Wufengyou T025 as the tested materials,the influences of straw returning with a microbial agent on the quantity of microorganisms and enzyme activity in rhizosphere soil in fields were studied,and soil productivity was tested with yield and agricultural traits of late rice. The results showed that straw returning with the microbial agent could significantly improve the quantity of bacteria,fungi and actinomyces in soil,enhance the activity of sucrase,urease,catalase and cellulase,and improve the number of grains per spike,setting percentage,thousand seed weight and yield of late rice. The combination of rice straw returning and the microbial agent has a good prospect of application. | Ni Guorong Tu Guoquan Wei Saijin Wu Jianfu Shi Qinghua Zhou Chunhuo Pan Xiaohua | 2017 | Meteorological and Environmental Research2017，8，6： | 3 |
| 3 | Changes in Protein Content and Chitinase and β-1,3- glucanase Activities of Rice with Blast Resistance Induced by Ag-antibiotic 702显示文摘[Objective] The paper was to explore the effect of rice blast resistance induced by Ag-antibiotics 702 on protein content and chitinase and β-1,3-glucanase activities in rice. [Method]At the fourth leaf stage of Luliangyou 996,15 μg/m L Ag-antibiotic 702 was sprayed,while Validamycin and distilled water were sprayed as positive control and negative control,respectively. All treatments were inoculated with spore fluid of Magnaporthe grisea at 48 h post spraying,and the rice inoculated with only distilled water was used as blank control. The enzymes activities( endochitinase,exochitinase and β-1,3-glucanase) and total protein content in rice leaves were determined every 24 h within 168 h post spraying. [Result] Compared with the blank control,the rice inoculated with spore fluid of M. grisea could significantly increase the total protein content and the activities of β-1,3-glucanase and chitinase. The induction effect of Ag-antibiotics 702 exceeded that of Validamycin treatment. And the changes in activities of β-1,3-glucanase and chitinase had obvious synchronicity. [Conclusion] Ag-antibiotic 702 can significantly improve the total protein content and the activities of β-1,3-glucanase and chitinase,thus enhancing the resistance to rice blast. | Hu Neng Tu Xiaorong Li Kuntai Ding Hao Li Huan Zhang Huawei Tu Guoquan Huang Lin | 2017 | Plant Diseases and Pests2017，8，4： | 2 |
| 4 | Optimization of medium composition for actinomycin X2 production by Streptomyces spp JAU4234 using response surface methodology显示文摘 | Xiong Zhiqiang Tu Xiaorong Tu Guoquan | 2008 | Journal of Industrial Microbiology and Biotechnology2008，35，7： | 1 |
| 5 | Field Efficacy of Agricultural Antibiotic 702 against Rice Leaf Blast and Panicle Blast显示文摘The control effects of agricultural antibiotic 702( ag-antibiotic 702) on rice leaf blast and panicle blast were evaluated in field trials. With ag-antibiotic702 and the two-line early hybrid rice 287 as test materials,different concentrations of ag-antibiotic 702 liquids were sprayed at tillering stage and full heading stage of rice. In leaf blast test,the disease indexes of leaf blast at tillering stage were surveyed at 1 d prior to the first spraying and at 15 d post spraying,respectively.The disease indexes( DI) and the rice yield in panicle blast test was investigated at milky stage. Meanwhile,enzyme activities,including peroxidase( POD),catalase( CAT),superoxide dismutase( SOD),phenylalanine ammonia-lyase( PAL),and polyphenol oxidase( PPO),were measured. Kasugamycin( 45 μg/m L)and deionized water were designed as positive and negative controls. When the concentrations of ag-antibiotic 702 were 15,30 and 45 μg/m L,the control effects against leaf blast were 72. 14%,80. 32% and 85. 22%,respectively; the control effects on panicle blast were 74. 08%,84. 87% and 86. 58%,respectively; and the yield increase rates were 17. 28%,21. 40% and 22. 57%,respectively. So,ag-antibiotic 702,as a new agricultural antibiotic fungicide,could effectively control rice blast by inducing defensive enzymes,and increase rice yield. The results will contribute to further development of ag-antibiotic 702 and its application in rice blast control. | Li Xunhang Tu Xiaorong Huang Lin Li Hanguang Tu Guoquan | 2016 | Plant Diseases and Pests2016，7，5： | 0 |
| 6 | Evaluation of 0.3% Agricultural Antibiotic 702 AS against Three Pathogenic Fungi of Rice显示文摘[Objective] The paper was to study control effect and bio-control potential of 0.3% agricultural antibiotic 702 aqueous solution(AS) against Rhizoctonia solani,Magnaporthe grisea and Ustilaginoidea virens. [Method] The toxicity effect of 0.3% agricultural antibiotic 702 AS against R. solani,M. grisea and U. virens were studied by mycelial growth rate method and mycelial wet weight method. [Result] The EC50 and EC90 values of agricultural antibiotics 702 AS to R.solani,M. grisea and U. virens in vitro condition were 4.16 and 16.17 mg/L,16.05 and 41.85 mg/L,111.2 and 389.0 mg/L,respectively. Agricultural antibiotics 702 AS had good inhibition effect against R. solani and M. grisea and could basically achieve the effects of commercially available Jinggangmycin and kasugamycin,but the inhibition effect against U. virens was relatively weak. The preventive activity of aqueous solution was obviously higher than treatment activity.[Conclusion] 0.3% Agricultural antibiotic 702 AS is considered to be a potential biological control agent against R. solani,M. grisea and U. virens,and can be used as the potential alternative pesticide of Jinggangmycin. | Tu Xiaorong Wei Saijin Cheng Xin Zhou Yun Tu Guoquan | 2013 | Plant Diseases and Pests2013，4，3： | 0 |
| 7 | Blast Resistance of Rice Induced by Ag-antibiotic 702显示文摘To explore rice blast resistance induced by Ag-antibiotic 702,different concentrations of Ag-antibiotic 702 were spayed on susceptible variety Luliangyou 996 at three-leaf and one-heart stage,to screen the optimal concentration for inducing rice blast resistance. Ag-antibiotic 702 was sprayed at seven different growth stages of rice,to determine the best growth stage for induced blast resistance and duration of blast resistance. Various treatments were inoculated with spore fluid of Magnaporthe grisea at 4 d post spraying,and disease index and incidence rate as well as induction effect of different treatments were investigated after 7 d.The results showed that six concentrations of Ag-antibiotic 702 could induce rice resistance against blast,and 15 μg/mL led to the highest blast resistance; spraying15 μg/mL Ag-antibiotic 702 at seven different growth stages could induce rice resistance against blast; the three-leaf and one-heart stage was the best growth stage for inducing rice blast resistance,and the relative induction effect reached 56. 56%; rice blast resistance was the highest at 48-96 h post spraying,and duration of induced rice blast resistance exceeded 144 h. The study will provide useful experimental data for further development of Ag-antibiotic 702 and application of prevention and control methods against rice blast. | Hu Neng Yang Shuai Lei Zhihuo Tu Xiaorong Tu Guoquan Huang Lin | 2017 | Plant Diseases and Pests2017，8，3： | 0 |
| 8 | Antifungal Activity and Toxicity of Two New types of Polyene Antibiotics against Four Species of Rice Pathogenic Fungi显示文摘The antifungal activity and toxicity of new type polyene macrolide antibiotics-Antifungalmycin 702 and Fungichromin against Rhizoctonia solani,Helminthosporium sigmoideum Cav. ,Magnaporthe grisea and Ustilaginoidea virens were studied by mycelial growth rate method and mycelial dry weight method in vitro. The results showed that both of Antifungalmycin 702 and Fungichromin had strong antifungal activity against four speices of rice pathogenic fungi. Treated with 50 μg / mL Antifungalmycin 702 ,the inhibitory rates of Antifungalmycin 702 against R. solani,H. sigmoideum Cav. ,M. grisea and U. virens were 100% 、 100% 、91. 20% and 68. 10% ,and EC 50 and EC 90 were 7. 00,10. 30,14. 41,26. 71 and 13. 20,17. 66,27. 67,128. 28 μg / mL,respectively. Treated with 13 μg / mL Fungichromin,the inhibitory rates of Fungichromin against four speices of rice pathogenic fungi were 78. 20% ,100% ,100% and 79. 17% ,and EC 50 and EC 90 were 1. 47,1. 91,2. 37,0. 20 and 41. 05,3. 92,4. 34,135. 54 μg / mL,respectively. The antifungal activity of Fungichromin was stronger than Antifungalmycin 702. | Hui LU Meiping WEN Saijin WEI Xiaorong TU Jia XU Guoquan TU | 2012 | Plant Diseases and Pests2012，3，3： | 0 |
| 9 | Inhibition Mechanism of Antifungal Monomer DZP8 against Rhizoctonia solani显示文摘In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani, the effects of DZP8 on mycelial morphology were investigated under light microscope, and the effects of DZP8 on mycelial inclusion leakage and cell membrane damage of mycelium were determined. The results showed that DZP8 caused a series of changes in mycelial morphology of R. solani in liquid culture condition. DZP8 treatments with concentrations of 1.81 and 3.35 μg/mL for 24 h caused big vacuole, rough surface and more inclusions of mycelium. With the treatment time prolonging, the mycelium distorted and appeared irregular constriction. DZP8 treatment with concentration of 20.10 μg/mL led to the increase of conductivity of mycelium culture liquid, leakage of soluble sugar and protein, and the lipid peroxidation of mycelium membrane. It was found that DZP8 at a very low concentration could cause changes of mycelial morphology of R. solani, while only a certain concentration could cause significant damage to cell membrane. This indicated that cell membrane might be one of the action sites of DZP8, and it might have other action sites or mechanism. | Lu Hui Wen Meiping Tu Xiaorong Xu Jia Feng Huagen Tu Guoquan | 2013 | Plant Diseases and Pests2013，4，1： | 0 |
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