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| 1 | YKL40 expression in CD14^+ liver cells in acute and chronic injury显示文摘AIM:To demonstrate that CD14 + cells are an important source of the growth factor YKL40 in acute and chronic liver damage.METHODS:Rats were inoculated with one dose of CCl4 to induce acute damage.Liver biopsies were obtained at 0,6,12,24,48 and 72 h.For chronic damage,CCl4 was administered three days per week for 6 or 8 wk.Tissue samples were collected,and cellular populations were isolated by liver digestion and purified by cell sorting.YKL40 mRNA and protein expression were evaluated by realtime polymerase chain reaction and western blot.RESULTS:Acute liver damage induced a rapid increase of YKL40 mRNA beginning at 12 h.Expression peaked at 24 h,with a 26fold increase over basal levels.By 72 h however,YKL40 expression levels had nearly returned to control levels.On the other hand,chronic damage induced a sustained increase in YKL40 expression,with 7and 9fold higher levels at 6 and 8 wk,respectively.The pattern of YKL40 expression in different subpopulations showed that CD14+cells,which include Kupffer cells,are a source of YKL40 after acute damage at 72 h[0.09 relative expression units(REU)]as well as after chronic injury at 6 wk(0.11 REU).Hepatocytes,in turn,accounted for 0.06 and 0.01 REU after 72 h(acute)or 6 wk(chronic),respectively.The rest of the CD14cells(including T lymphocytes,B lymphocytes,natural killer and natural killer T cells) yielded 0.07 and 0.15 REU at 72 h and 6 wk,respectively.YKL40 protein expression in liver was detected at 72 h as well as 6 and 8 wk,with the highest expression relative to controls(11fold;P≤0.05)seen at 6 wk.Macrophages were stimulated by lipopolysaccharide.We demonstrate that under these conditions,these cells showed maximum expression of YKL40 at 12 h,with P<0.05 compared with controls.CONCLUSION:Hepatic CD14 + cells are an YKL40 mRNA and protein source in acute and chronic liver injury,with expression patterns similar to growth factors implicated in inflammationfibrogenesis. | Oscar Pizano-Martínez Irinea Yaez-Sánchez Pilar Alatorre-Carranza Alejandra Miranda-Díaz Pablo C Ortiz-Lazareno Trinidad García-Iglesias Adrian Daneri-Navarro Mónica Vázquez-Del Mercado Mary Fafutis-Morris Vidal Delgado-Rizo | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | Taste and mouth-feel properties of different types of tannin-like polyphenolic compounds and anthocyanins in wine显示文摘 | Stéphane Vidal Leigh Francis Ann Noble Mariola Kwiatkowski Véronique Cheynier Elizabeth Waters | 2003 | Analytica Chimica Acta2003，，1： | 2 |
| 3 | Pseudomonas aeruginosa in patients hospitalised for COPD exacerbation: a prospective study 显示文摘 | Garcia Vidal C Almagro P Romani V | 2009 | Eur Respir J2009，34，5： | 1 |
| 4 | Chemical Modifications and Study of Structure - Function Interactions in Proteins 显示文摘 | Vidal V Cases E Cuq J L | 2003 | OCL - Oleagineux Crops Gras Lipides2003，10，1： | 1 |
| 5 | Effects of systemic steroids in patients with severe community - acquired pneumonia 显示文摘 | Garcial - Vidal C Calbo E Pascual V | 2007 | Eur Respir J2007，30，5： | 1 |
| 6 | Develop- ment and field test of an on-line computerized instrumenta- tion system for air velocity, temperature and differential pressure measurements in poultry houses 显示文摘 | Blanes Vidal V Guijarro E Nadimi A G | 2010 | Spanish Journal of Agricultural Research2010，8，3： | 1 |
| 7 | Peripherally inserted central catheters (PICC):a promising technique显示文摘 | Vidal V Jacquier A Monnet O | 2008 | J Radiol2008，89，781： | 1 |
| 8 | Bronchial artery embolization in adults with cystic fibrosis: impact on the clinical course and survival显示文摘 | Vidal V Therasse E Berthiaume Y | 2006 | J Vasc Interv Radiol2006，17，： | 1 |
| 9 | Prospective evaluation of PICC line related,complications显示文摘 | Vidal V Muller C Jacquier A | | 0，，04： | 1 |
| 10 | Activation of the Supplementary Motor area and of At tentional Networks During Temporal Processing显示文摘 | Macar F Lejeune H Bonnet M Ferrara A Poutbas V Vidal F | 2002 | Experimental Brain Research2002，142，4： | 1 |
| 11 | Angiogenesis in normal and neoplastic pituitary tissues 显示文摘 | LLOYD R V VIDAL S HORVATH E | 2003 | Microsc Res Tech2003，60，2： | 1 |
| 12 | Modification of oxygen storage capacity of CeO2-ZrO2 mixed oxides after redox cycling aging 显示文摘 | Perrichon V Vidal H | 2000 | Catalysis Today2000，59，： | 1 |
| 13 | Prospective evaluation of PICC line related complications显示文摘 | Vidal V Muller C Jaequier A | 2008 | J Radiot2008，89，4： | 1 |
| 14 | Study of the contribu- tion of cobalt additive to the behavior of nickel oxy-hydroxide electrode by the potential-dynamic techniques显示文摘 | PRALONG V CHABRE Y VIDAL A | 2002 | Solid State Ionics2002，147，1： | 1 |
| 15 | Variations of the Hawaiian hot spot activity revealed by variations in the magma production rate显示文摘 | VIDAL V BONNEVILLE A | 2004 | J Geophys Res2004，109，03104： | 1 |
| 16 | Peripherally insert- ed central catheter: a therapeutic tool 显示文摘 | Vidal V Cohen F Casalonga F | 2009 | Presse Med2009，38，4： | 1 |
| 17 | A complete α1,3-galactosyltransferase gene is present in the human genome and partially transcribed显示文摘 | Lantéri M Giordanengo V Vidal F | 2002 | Glycobiology2002，12，12： | 1 |
| 18 | Assessing soil surface roughness decay during simulated rainfall by multifractal analysis显示文摘 | Vidal V~tzquez E Moreno R G Miranda J | 2008 | Nonlinear Processes in Geophysics2008，15，3： | 1 |
| 19 | Identification of interactions among casein gels using dissociating chemical agents显示文摘 | LEFEBVRE-CASES E GASTALDI E VIDAL V | 1998 | J Dairy Sci1998，81，： | 1 |
| 20 | PI3K/mTOR dual inhibitor VS-5584 preferentially targets cancer stem cells显示文摘 | KOLEV V N WRIGHT Q G VIDAL C M | 2015 | Cancer Res2015，5，2： | 1 |
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