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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Life stages and reproductive mar-morkrebs(Marbled crayfish)the first parthenogenetic decapodcrustacean显示文摘 | Vogt G Tolley L Scholtz G | 2004 | Morphol2004，261，： | 1 |
| 3 | Inhibition of cyclooxygenase-2 prevents inflammafion-mediated preterm labor in the mouse显示文摘 | Gross G Imamura T Vogt S K | 2000 | Am J Physiol Regul Integr Comp Physiol2000，278，： | 1 |
| 4 | Rotary cell culture system(RCCS):A new method for cultivating hepatocytes on microcarriers显示文摘 | Mitteregger R Vogt G Rossmanith E | 1999 | Int J Artif Organs1999，22，12： | 1 |
| 5 | Comparison of the influence of straight and curved mould continuous-casting machines on product quality显示文摘 | Vom Ende H Vogt G | 1972 | JISI1972，210，12： | 1 |
| 6 | Toughness Requirements are Studied for Large Diameter Gas Pipelines 显示文摘 | VOGT G H | 1984 | Oil and Gas Journal1984，182，10： | 1 |
| 7 | Efficacy of three citrus oil formulations against Solenopsis invicta Buren (Hymenoptera: Formicidae), the red imported fire ant 显示文摘 | Vogt J T Shelton T G Merchant M E | 2002 | J Agric Urban Entomol2002，19，3： | 1 |
| 8 | Monitoring Drought Conditions and Their Uncertainties in Africa Using TRMM Data显示文摘 | Naumann G Barbosa P Carrao H Singleton A Vogt J | 2012 | EN2012，，10： | 1 |
| 9 | Toughness Requirements Are Studied for large - diameter Gas Pipelines显示文摘 | VOGT G H | 1984 | OGJ Aug 131984，82，33： | 1 |
| 10 | Fusion via a linear combination of scores显示文摘 | Vogt C C Cottrell G W | 1999 | Information Retrieval1999，1，3： | 1 |
| 11 | Uhrasmall superparamagnetic iron oxide-enhanced MR imaging of atherosclerotic plaque in hyperlipidemic rabbits 显示文摘 | Ruehm S G Corot C Vogt P | 2002 | Acad Radiol2002，9，1： | 1 |
| 12 | Whole-bodydual-modality PET/CT and whole-body MRI for tumor staging in oncology 显示文摘 | Antoch G Vogt FM Freudenberg LS | 2003 | JA- MA2003，290，24： | 1 |
| 13 | Whole-body Dual-modality PET/CT and Whole-body MRI for Tumor Staging in Oncology显示文摘 | Antoch G Vogt FM Freudenberg LS | 2003 | JAMA2003，290，24： | 1 |
| 14 | Coexpression of CD4 and CD8alpha on rat T cells in whole blood= a sensitive marker for monitoring T cell immunosuppressive drugs显示文摘 | Diaz-Romero J Vogt G Weckbecker G | 2001 | J Immunol Methods2001，254，12： | 1 |
| 15 | Odorant-binding protein subfamilies associate with distinct classes of olfac- tory receptor neurons in insects显示文摘 | Vogt R G Prestwich G D Lerner M R | 1991 | J Neurobiol1991，22，： | 1 |
| 16 | Neural circuits underlying imitation learning of hand actions: an event-related fMRI study显示文摘 | Buccino G Vogt S Ritzl A | 2004 | Neuron2004，42，： | 1 |
| 17 | Life-cycle and functional cytology of the hepatopancreatic cells of Astacus astacus (Crustacea, Decapoda)显示文摘 | VOGT G | 1994 | Zoomorphology1994，114，： | 1 |
| 18 | In vivo decondensation of chromatin and nucleolar fibrillar component by Leucaena leucocephala ingredient显示文摘 | | 1991 | Biol Cell1991，72，： | 1 |
| 19 | Grape seed extract as antioxidant in cooked,cold stored turkey meat显示文摘 | Mielnik M B Olsen E Vogt G | 2006 | LWT -Food Science and Technology2006，39，3： | 1 |
| 20 | In inland China, rice, rather than fish, is the major pathway for me?thylmercury exposure显示文摘 | Zhang H Feng X Larssen T Qiu G Vogt R D | 2010 | Environ Health Perspect2010，118，9： | 1 |
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