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| 1 | 间充质的干细胞的隔离和描述源于人的胎盘组织显示文摘 AIM: To explore the feasibility of placenta tissue as a reliable and efficient source for generating mesenchymal stem cells (MSC). METHODS: MSC were generated from human placenta tissue by enzymatic digestion and mechanical dissociation. The placenta MSC (PLC-MSC) were characterized for expression of cell surface markers, embryonic stem cell (ECS) gene expression and their differentiation ability into adipocytes and osteocytes. The immunosuppressive properties of PLC-MSC on resting and phytohemagglutinin (PHA) stimulated allogenic T cells were assessed by means of cell proliferation via incorporation of tritium thymidine (3H-TdR). RESULTS: The generated PLC-MSC appeared as spindle-shaped cells, expressed common MSC surface markers and ESC transcriptional factors. They also differen-tiated into adipogenic and osteogenic lineages when induced. However, continuous cultivation up to passage 15 caused changes in morphological appearance and cellular senescence, although the stem cell nature of their protein expression was unchanged. In terms of their immunosuppressive properties, PLC-MSC were unable to stimulate resting T cell proliferation; they inhibited the PHA stimulated T cells in a dose dependent manner through cell to cell contact. In our study, MSC generated from human placenta exhibited similar mesenchymal cell surface markers; MSC-like gene expression pattern and MSC-like differentiation potential were comparable to other sources of MSC. CONCLUSION: We suggest that placenta tissues can serve as an alternative source of MSC for future experimental and clinical studies. | Shalini Vellasamy Pratheep Sandrasaigaran Sharmili Vidyadaran Elizabeth George Rajesh Ramasamy | 2012 | World Journal of Stem Cells2012，4，6： | 5 |
| 2 | 骨头的 Immunophenotype 和区别能力从 CBA/Ca, ICR 和 Balb/c 的导出髓的间充质的干细胞老鼠显示文摘 AIM:To assess the capacity to isolate and expand mesenchymal stem cells(MSC)from bone marrow of CBA/Ca,ICR and Balb/c mice. METHODS:Bone marrow of tibia and femur were flushed,cultured and maintained in supplemented Dulbecco’s modified Eagle’s medium.MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106,Sca-1 and CD44 and negativity to CD45,CD11b and MHC classⅡ.Differentiation capacity of MSC towards osteogenic and adipo-genic lineages were also assessed. RESULTS:MSC were successfully cultured from bone marrow of all 3 strains,albeit differences in the temporal expression of certain surface antigens.Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype(CD106-CD45+CD11b+Sca-1low)to typical MSC phenotype(CD106+CD45-CD11b-Sca-1high)with increasing passages. CONCLUSION:Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation. | Yin Yin Ooi Zul'atfi Rahmat Shinsmon Jose Rajesh Ramasamy Sharmili Vidyadaran | 2013 | World Journal of Stem Cells2013，5，1： | 3 |
| 3 | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at GO phase of cell cycle显示文摘 | Vellasamy S Sandrasaigaran P Vidyadaran S | 2013 | Cell Biol Int2013，37，3： | 1 |
| 4 | Weight, volume and surface area of placenta of nor- mal pregnant women and their relation to maternal and neonatal parameters in Malay, Chinese and Indian eth- nic groups显示文摘 | SIVARAO S VIDYADARAN M K JAMMAL A B | 2002 | Placenta2002，23，89： | 1 |
| 5 | Mesenchymal stem cells of human placenta and umbilical cord suppress T-cell proliferation at G (0) phase of cell cycle显示文摘 | Vellasamy S Sandrasaigaran P Vidyadaran S | 2013 | Cell Biol Int2013，37，3： | 1 |
| 6 | Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide显示文摘 | Yin Yin Ooi Rajesh Ramasamy Zul’atfi Rahmat Hemavathy Subramaiam Shi Wei Tan Maha Abdullah Daud Ahmad Israf Sharmili Vidyadaran | 2010 | International Immunopharmacology2010，，12： | 1 |
| 7 | Mesenchymal stem cells inhibit proliferation of lymphoid origin haematopoietic tumour cells by inducing cell cycle arrest显示文摘 | SARMADI V H TONG C K VIDYADARAN S | 2010 | Med J Malaysia2010，65，3： | 1 |
| 8 | Cardamonin from Alpinia rafflesiana inhibits inflammatory responses in IFN-gamma/LPS- stimulated BV2 microglia via NF-kappa B signalling pathway显示文摘 | Chow Y L Lee K H Vidyadaran S | 2012 | Int Immunopharmacol2012，12，4： | 1 |
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