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| 1 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 2 | The repeatability of superovulatory response and embryo recovery in sheep显示文摘 | Bari F Khalid M Wolf B Haresigh W Murray A Merrell B | 2001 | Theriogenology2001，56，1： | 1 |
| 3 | Linear and nonlinear state - space controllers for magnetic levitation 显示文摘 | W Barie J Chiasson | 1996 | International Journal of System Science1996，，11： | 1 |
| 4 | Factors affecting the survival of sheep embryos after transfer within a MOET program显示文摘 | Bari F Khalid M Haresign W | 2003 | Theriogenology2003，59，： | 1 |
| 5 | Effects of preheating of crude palm oil (CPO) on injection system, performance and emission of a diesel engine显示文摘 | Bari S Lim T H Yu C W | 2002 | Renewable Energy2002，27，3： | 1 |
| 6 | Development of a new revised version ofthe Witczak E * predictive model for hot mix asphalt mixtures显示文摘 | Bari J Witczak M W | 2006 | Journal of the Association of Asphalt Paving Technologists2006，75，： | 1 |
| 7 | Changes in the color,chemical stability and antioxidant capacity of thermally treated anthocyanin aqueous solution over storage显示文摘 | Sui X Bary S Zhou W | 2016 | Food Chemistry2016，192，： | 1 |
| 8 | A process for primary migration of petroleum显示文摘 | BARY E E FOSTER W R | 1980 | AAPG1980，64，1： | 1 |
| 9 | Development of a new revised version of the witczak E*predictive model for hot mix asphalt mixtures显示文摘 | Bari J Witczak M W | 2006 | Association of Asphalt Paving Technologist(AAPT)2006，75，： | 1 |
| 10 | The preparationof mesoporphyrin]X and etioporphyrin ID1 显示文摘 | Bari W Baker Michele Ruccia Alsoph H Corwin | 1964 | AnalyticalBiochemistry1964，8，： | 1 |
| 11 | Factors affecting the survival of sheep embryos after transfer within a MOET program显示文摘 | Bari F Khalid M Haresign W | 2003 | Theriogenology2003，59，56： | 1 |
| 12 | Contaminants in the Canadian Arctic: 5 years of progress in understand- ing sources, occurence and pathways显示文摘 | Macdonaid R W Barie L A Bidleman T F | 2000 | The Science of the total Environment2000，254，23： | 1 |
| 13 | Source apportionment of indoor and outdoor volatile organic compounds at homes in Edmonton,Canada显示文摘 | BARI M A KINDZIERSKI W B WHEELER A J | 2015 | Build Environ2015，90，： | 1 |
| 14 | New Predictive Models for the Viscosity and Complex Shear Modulus of Asphalt Binders:for Use with Mechanistic-empirical Pavement Design Guide 显示文摘 | BARI J WITCZAK M W | 2007 | Transportation Research Record2007，，2001： | 1 |
| 15 | Effect of fuel injection timing with waste cooking oil as a fuel in a direct injection diesel engine显示文摘 | Bari S Yu C W Lim T H | 2004 | Journal of Automobile Engineering2004，218，1： | 1 |
| 16 | The origins and genetic structure of three co-resident Chinese Muslim populations: the Salar, Bo'an and Dongxiang 显示文摘 | Wang W Wise C Barie T | 2003 | Hum Genet2003，113，3： | 1 |
| 17 | Development of a New Revised Version of the Witczak E * Predictive Model for Hot Mix Asphalt Mixtures 显示文摘 | BARI J WITCZAK M W | 2006 | Journal of the Association of Asphalt Paving Technologists2006，75，： | 1 |
| 18 | The importance of blood lactate clearance as a predictor of early mortality following the modified Norwood procedure显示文摘 | Bari M Douglas W ZdenkaR | | 0，，05： | 1 |
| 19 | Shear wave elastography imaging of carotid plaques: feasible, reproducible and of clinical potential 显示文摘 | KUMAR V R JAMES W G BARIS K | 2014 | Cardiovascular Ultrasound2014，12，： | 1 |
| 20 | Factors affecting the survival of sheep embryos after transfer within a MOET program 显示文摘 | BARI F KHALID M HARESIGN W | 2003 | Theriogenology2003，59，56： | 1 |
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