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| 1 | Identification of Porphyra lines using computerized DNA fingerprinting显示文摘RAPD (Randomly Amplified Polymorphic DNA) analysis was performed with filaments of 15 Porphyra lines representing four important groups (P. yezoensis, P. haitanensis, P. katadai var. Hemiphylla and P. digospermatangia). Eight stable and repeatable RAPD bands amplified with two primers, OPN-02 and OPJ-18, were selected for the construction of DNA fingerprinting. The RAPD results were scored based on the presence or absence of each of the 8 bands and then converted to computer language expressed with two digitals, 1 and 0, which represented the presence (numbered as 1) or absence (numbered as 0) of each band, respectively. Based on these results, a model DNA fingerprint and a computerized DNA fingerprint were constructed. In the constructed DNA fingerprint, each Porphyra line has its unique fingerprinting pattern and can be easily distinguished from each other. Later, a software, named as PhGI, was designed based on this DNA fingerprinting. It can be used in practical Porphyra line identification. | WangBin, Jia Jianhang, Shi Jinfeng, Chen Yihua, JinDemin, Xu Pu, Mei Junxue, Weng Manli 1. Institute of Genetics, Chinese Academy of Sciences, Beijing 100101 (E-mail: mlweng@genetics. ac. cn), China 2. Jiangsu Marine Fishery Institute, Nan | 2001 | Acta Oceanologica Sinica2001，20，3： | 7 |
| 2 | Development of EST-SSR primers and their practicability test for Laminaria显示文摘Laminaria (Saccharina) is one of the economic important seaweeds.Through sequence analysis of 4 099 ESTs from Laminaria (971 were generated from our own cDNA laboratories and 3 128 were downloaded from updated EST databases) with SSRIT software,two hundred and fifty-four SSRs in 206 ESTs were found.From the 254 SSRs,sixty-three SSR primer-pairs were designed. In order to test their practicability, the 63 primer-pairs were tested in commonly used SSR reaction conditions using 12 Laminara DNA samples as templates. The results show that 23 SSR primer-pairs gave good amplification patterns on most (more than 80%) of the 12 Laminara DNA templates. Genetic diversity study of 12 Laminaria lines,which were widely used in breeding and economic cultivation in China,was performed based on the obtained SSR data. | WANG Guoliang TAN Xiaolong SHEN Junling LI Jian ZHANG Lei SUN Jianwei WANG Bin WENG Manli LIU Tao | 2011 | Acta Oceanologica Sinica2011，30，3： | 3 |
| 3 | Rice seed identification by computerized AFLP-DNA fingerprinting显示文摘Wedevelopedacomputerizedseedidentificationsystem.FifteenricevarietiesthatwerewidelyusedinChinawereanalyzedbyAFLPfingerprinting.12primerpairswerescreened.Inordertosimplifytheprocedureandcutdownthecostinseedidentification,theleastnumberofprimerpairsforpracticalseedidentifi cationshouldbeselected. | CHEN Yihua JIA Jianhang LI Chuanyou WANG Bin WENG Manli Inst of Genetics,Chinese Academy of Sci,Beijing 100101,China | 2000 | Chinese Rice Research Newsletter2000，8，1： | 2 |
| 4 | Characterization of multiple cold induced genes from Ammopiptanthus mongolicus and functional analyses of gene AmEBP1显示文摘 | Pengxiu Cao Jian Song Chunjiang Zhou Manli Weng Jing Liu Fuxin Wang Feng Zhao Dianqi Feng Bin Wang | 2009 | Plant Molecular Biology2009，，5： | 1 |
| 5 | Mutations in the Bacillus subtilis Purine Repressor That Perturb PRPP Effector Function In Vitro and In Vivo显示文摘 | Manli Weng Howard Zalkin | 2000 | Current Microbiology2000，，1： | 1 |
| 6 | Comparative Studies on Preparation of Large Plant DNA Fragments by Pulse Field Gel Electrophoresis显示文摘The preparation of large plant DNA fragments is extremely important to the construction of large insert DNA libraries (YAC, BAC, PAC and TAC). Although several techniques have been developed in each step of large plant DNA fragments preparation, the whole processing remains complicated and difficult. Based on authors research experience and the recent worldwide development in this field, the following aspects are discussed in this paper: techniques of plant high molecular weight (HMW) DNA purification by pre-electrophoresis, the optimal conditions for the partial digestion of the HMW DNA by HindIII, the isolation effects of of large plant DNA fragments (100~400 kb) with different parameters of pulse field gel electrophoresis (PFGE), and the recovery of large DNA fragments. Through comparative studies, the advantages and disadvantages of each technique are discussed and some recommendations are proposed for preparing high quality large plant DNA fragments. The suggested techniques have been used in preparing the large DNA fragments of maize, rice, moss, laver, sea tangle and peach,and similar results are obtained among all the materials. This paper only reports the results using maize as material. | 杨继良 王庆华 Deng Daiyong Yang Dianer JIN Demin WENG Manli ZHANG Juren Wang Bin | 2004 | High Technology Letters2004，10，1： | 1 |
| 7 | Aberrant single-subject morphological brain networks in first-episode,treatment-naive adolescents with major depressive disorder显示文摘Background:Neuroimaging-based connectome studies have indicated that major depressive disorder(MDD)is associated with dis-rupted topological organization of large-scale brain networks.However,the disruptions and their clinical and cognitive relevance are not well established for morphological brain networks in adolescent MDD.Objective:To investigate the topological alterations of single-subject morphological brain networks in adolescent MDD.Methods:Twenty-five first-episode,treatment-naive adolescents with MDD and 19 healthy controls(HCs)underwent T1-weighted magnetic resonance imaging and a battery of neuropsychological tests.Single-subject morphological brain networks were constructed separately based on cortical thickness,fractal dimension,gyrification index,and sulcus depth,and topologically characterized by graph-based approaches.Between-group differences were inferred by permutation testing.For significant alterations,partial correla-tions were used to examine their associations with clinical and neuropsychological variables in the patients.Finally,a support vector machine was used to classify the patients from controls.Results:Compared with the HCs,the patients exhibited topological alterations only in cortical thickness-based networks character-ized by higher nodal centralities in parietal(left primary sensory cortex)but lower nodal centralities in temporal(left parabelt complex,right perirhinal ectorhinal cortex,right area PHT and right ventral visual complex)regions.Moreover,decreased nodal centralities of some temporal regions were correlated with cognitive dysfunction and clinical characteristics of the patients.These results were largely reproducible for binary and weighted network analyses.Finally,topological properties of the cortical thickness-based net-works were able to distinguish the MDD adolescents from HCs with 87.6%accuracy.Conclusion:Adolescent MDD is associated with disrupted topological organization of morphological brain networks,and the disrup-tions provide potential biomarkers for diagnosing and monitoring the disease. | Xiaofan Qiu Junle Li Fen Pan Yuping Yang Weihua Zhou Jinkai Chen Ning Wei Shaojia Lu Xuchu Weng Manli Huang Jinhui Wang | 2023 | Psychoradiology2023，3，1： | 0 |
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