|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Evaluation and identification of electrical and thermal conduction me- chanisms in carbon nanotube/epoxy composites 显示文摘 | C ojny F H Wichmann M Fiedler H B | 2006 | Poly- mer2006，47，8： | 1 |
| 3 | Influence of different carbon nanotubes on the mechanical properties of epaxmatrix composites-a comparative study显示文摘 | GOJNY F H WICHMANN M H G FIEDLER B | 2005 | Composites Science and Technology2005，65，15116： | 1 |
| 4 | Genes and lifestyle factors in obesity:Results from 12462 subjects from MONICA/KORA显示文摘 | Holzapfel C Grallert H Huth C Wahl S Fischer B Doring A Ruckert I M Hinney A Hebebrand J Wichmann H E Hauner H Illig T and Heid I M | | 0，，10： | 1 |
| 5 | A case of se- vere Ebola virus infection complicated by gram-negative sep- ticemia显示文摘 | Kreuels B Wichmann D Emmerich P | 2014 | N Engl J Med2014，371，25： | 1 |
| 6 | Test of the utility of apple retrotransposon insertion patterns for molecular identification of Jonathan somatic mutants显示文摘 | GALLI Z S WICHMANN B KISS E SZABO T HESZKY L | 2008 | J Hortic Sci2008，14，： | 1 |
| 7 | Evaluation and identification of electrical and thermal conduction mechanisms in carbon nanotuhe/epoxy composites显示文摘 | Gojny F H Wichmann M H G Fiedler B | 2006 | Polymer2006，47，： | 1 |
| 8 | Carbon nanotube reinforced epoxy composites enhanced stiffness and fracture toughness at low nanotube contents显示文摘 | Gojny F H Wichmann M H G Kopke U Fiedler B Schulte K | 2004 | Composites Science and Technology2004，64，： | 1 |
| 9 | Promiscuity in transgenicplants显示文摘 | BERGELSON J PURRINGTON C B WICHMANN G | 1998 | Nature1998，，395： | 1 |
| 10 | Influence of nano-modification on the mechanical and electri- cal properties of conventional fibre-reinforced composites 显示文摘 | GOJNY F H WICHMANN M H G FIEDLER B | 2005 | Composites Part A2005，36，11： | 1 |
| 11 | Two new Loci for body-weight regulation identified in a joint analysis of genome-wide association studies for early-onset extreme obesity in French and german study groups显示文摘 | Scherag A Dina C Hinney A Vatin V Scherag S Vogel C I Muller T D Grallert H Wichmann H E Balkau B Heude B Jarvelin M R Hartikainen A L Levy-Marchal C Weill J Delplanque J Korner A Kiess W Kovacs P Rayner N W Prokopenko I McCarthy M I Schafer H Jarick I Bo | | 0，，04： | 1 |
| 12 | Evaluation and identification of electrical and thermal conduction mechanisms in carbon nanotube/epoxy composites显示文摘 | Go]ny F H Wichmann M H G Fiedler B | 2006 | Polymer2006，47，6： | 1 |
| 13 | Evaluation and identification of electrical and thermal conduction mech- anisms in carbon nanotube/epoxy composites显示文摘 | Gojny F H Wichmann M H G Fiedler B | 2006 | Polymer2006，47，： | 1 |
| 14 | Machine Learning Ap- pried to Perception:Decision-Images for Gender Classification 显示文摘 | Wichmann FA Graf A Simoncelli EP B u lthoffHH & Sch?lkopfB | 2005 | Advances in Neural Information Processing Systems2005，17，： | 1 |
| 15 | Promiscuity in transgenic plants显示文摘 | Bergelson J Purrington C B Wichmann G | 1998 | Nature1998，395，： | 1 |
| 16 | Influ- ence of different carbon nanotubes on the mechanical prop- erties of epoxy matrix composites -a comparative study 显示文摘 | Gojny F H Wichmann M H G Fiedler B | 2005 | Composite Science and Technology2005，65，： | 1 |
| 17 | Classification of faces in man and machine显示文摘 | Graf A B A Wichmann F A Bulthoff H H | 2006 | Neural Computation2006，18，1： | 1 |
| 18 | E- valuation and identification of electrical and thermal con- duction mechanisms in carbon nanotube/epoxy composites 显示文摘 | GOJNY F H WICHMANN M H G FIEDLER B | 2006 | Polymer2006，24，6： | 1 |
| 19 | An efficient and portable pseudorandom number generator显示文摘 | Wichmann B A Hill I D | 1982 | Appl Stat1982，31，： | 1 |
| 20 | Fundamental aspects of nano-reinforced composites显示文摘 | Fiedler B Gojny F H Wichmann M H G | 2005 | Composites Science and Technology2005，14，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员