|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Hantaan virus infection causes an acute neurological disease that is fatal in adult laboratory mice显示文摘 | Wichmann D Grone HJ Frese M | 2002 | J Virol2002，76，17： | 1 |
| 3 | Genome wide association (GWA) study for early onset extreme obesity supports the role of fat mass and obesity associated gene (FTO) variants显示文摘 | Hinney A Nguyen T T Scherag A Friedel S Bronner G Muller T D Grallert H Illig T Wichmann H E Rief W Schafer H and Hebebrand J | | 0，，12： | 1 |
| 4 | Lack of Association Between the MEF2A Gene and Myocardial Infarction显示文摘 | Wolfgang Lieb Bj?rn Mayer Inke R. K?nig Iris Borwitzky Anika G?tz Silke Kain Christian Hengstenberg Patrick Linsel-Nitschke Marcus Fischer Angela D?ring H -Erich Wichmann Thomas Meitinger Reinhold Kreutz Andreas Ziegler Heribert Schunkert Jeanette Erdmann | 2008 | Circulation2008，，2： | 1 |
| 5 | Old proteins - new lo- cations : myoglobin, haemoglobin, neuroglobin and cytoglobin in solid tumours and cancer ceils显示文摘 | Gorr TA Wichmann D Pilarsky C | 2011 | Acta Physiol (Oxf)2011，202，3： | 1 |
| 6 | Incidenceandmor- tality of severesepsis insurgical intensive carepatients: the influ- ence of patient gender on diseaseprocess andoutcome显示文摘 | Wichmann MW Inthorn D Andress H J | 2000 | Intensive Care Med2000，26，2： | 1 |
| 7 | A case of se- vere Ebola virus infection complicated by gram-negative sep- ticemia显示文摘 | Kreuels B Wichmann D Emmerich P | 2014 | N Engl J Med2014，371，25： | 1 |
| 8 | Two new Loci for body-weight regulation identified in a joint analysis of genome-wide association studies for early-onset extreme obesity in French and german study groups显示文摘 | Scherag A Dina C Hinney A Vatin V Scherag S Vogel C I Muller T D Grallert H Wichmann H E Balkau B Heude B Jarvelin M R Hartikainen A L Levy-Marchal C Weill J Delplanque J Korner A Kiess W Kovacs P Rayner N W Prokopenko I McCarthy M I Schafer H Jarick I Bo | | 0，，04： | 1 |
| 9 | Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis显示文摘 | Wichmann D Poppert S Von Thien H | 2013 | BMC Infect Dis2013，13，： | 1 |
| 10 | Incidence and mortality of severe sepsis in surgical intensive care patients:the influence of patient gender on disease process and outcome显示文摘 | Wichmann MW Inthorn D Andress HJ | 2000 | Intensive Care Med2000，26，2： | 1 |
| 11 | A perfabricated precision attachment 3 years of experience with the Swiss M ini-SG system显示文摘 | Zajc D Wichmann M Reich S | 2007 | Int J Prosthodont2007，20，4： | 1 |
| 12 | An efficient and portable pseudorandom number generator显示文摘 | Wichmann B A Hill I D | 1982 | Appl Stat1982，31，： | 1 |
| 13 | The significance of interactions in group 11 complexes with N-heterocyclic carbenes显示文摘 | Nemcsok D Wichmann K Frenking G | 2004 | Organometallics2004，23，15： | 1 |
| 14 | Impo- hteness revisited: with special reference to dynamic and prosodic aspects 显示文摘 | CULPEPER J D BOUSFIELD A WICHMANN | 2003 | Journal of Pragmatics2003，，35： | 1 |
| 15 | Invasive pulmonary aspergillosis in the ICU:an emerging disease显示文摘 | Wichmann D Kluge S | 2013 | Intensive Care Med2013，39，4： | 1 |
| 16 | Increased plasma viscosity during an air pollution episode:a link to mortality显示文摘 | Peters A D(o)ring A Wichmann HE | 1997 | Lancet1997，349，： | 1 |
| 17 | Effect of protein A immunoadsorption on T cell activation in patients with inflammatory dilated cardiomyopathy显示文摘 | Bulut D Scheeler M Wichmann T | | 0，，10： | 1 |
| 18 | Web applica- tion tests with selenium显示文摘 | Bruns A Kornstadt A Wichmann D | 2009 | Software IEEE2009，26，5： | 1 |
| 19 | A case of severe Ebola virus infection complicated by gram-negative septicemia显示文摘 | Kreuels B Wichmann D Emmerich P | 2014 | N Engl J Med2014，371，25： | 1 |
| 20 | Clinical features of critically ill patients with Shiga toxin-induced hemolytic uremic syndrome 显示文摘 | Braune S A Wichmann D Von Heinz M C | 2013 | Critical Care Medicine2013，41，7： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员