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| 1 | Role of HSP-90 for increased nNOS-mediated vasodilation in mesenteric arteries in portal hypertension显示文摘AIM:To explore the role of heat shock protein-90 (HSP-90) for nitrergic vasorelaxation in the splanchnic circulation in rats with and without portal hypertension. METHODS: Neuronal nitric oxide synthase (nNOS) and HSP-90 were analyzed by immunofluorescence, western blotting and co-immunoprecipitation in the mesenteric vasculature and isolated nerves of portal-vein-ligated (PVL) rats and sham operated rats. In vitro perfused de-endothelialized mesenteric arterial vasculature was preconstricted with norepinephrine (EC80) and tested for nNOS-mediated vasorelaxation by periarterial nerve stimulation (PNS, 2-12 Hz, 45V) before and after incubation with geldanamycin (specific inhibitor of HSP-90 signalling, 3 μg/mL) or L-NAME (non-specific NOSblocker, 10-4 mol/L). RESULTS: nNOS and HSP-90 expression was significantly increased in mesenteric nerves from PVL as compared to sham rats. Moreover, nNOS and HSP-90 were visualized in mesenteric nerves by immunofluorescence and immunoprecipitation of nNOS co-immunoprecitated HSP-90 in sham and PVL rats. PNS induced a frequencydependent vasorelaxation which was more pronounced in PVL as compared to sham rats. L-NAME and geldanamycin markedly reduced nNOS-mediated vasorelaxation abrogating differences between the study groups. The effect of L-NAME and geldanamycin on nNOS-mediated vasorelaxation was significantly greater in PVL than in sham animals. However, no difference in magnitude of effect between L-NAME and geldanamycin was noted. CONCLUSION: HSP-90 acts as a signalling mediator of nNOS-dependent nerve mediated vascular responses in mesenteric arteries, and the increased nitrergic vasorelaxation observed in portal hypertension is mediated largely by HSP-90. | Lukas Moleda Lars Jurzik Matthias Froh Erwin Gbele Claus Hellerbrand Rainer H Straub Jürgen Schlmerich Reiner Wiest | 2010 | World Journal of Gastroenterology2010，16，15： | 4 |
| 2 | Adolescent depres- sion: Links toacademic coping and perceived auton- omysuport显示文摘 | Wnog E H Wiest D J | 1999 | Education1999，，4： | 1 |
| 3 | The effects of betamimetic drugs used for tocolysis on the fetal myocardium显示文摘 | Weidinger H Wiest W Schleich A | 1976 | J Perinat Med1976，4，4： | 1 |
| 4 | Adolescent depression:Links to academic coping and perceived autonomy support显示文摘 | Wong E H Wiest D J | | 0，，04： | 1 |
| 5 | Adolescent depression:links to academic coping and perceived autonomy support显示文摘 | Wiest D J | 1999 | Education1999，119，4： | 1 |
| 6 | In situ apoptotic and proliferation index in laryngealsquamous cell carcinomas显示文摘 | Hagedorn H G Tubel J Wiest I | 1998 | Anal Cell Pathol1998，16，3： | 1 |
| 7 | Adolescent depression:links to academic cop- ing and perceived autonomy support显示文摘 | Wong E H Wiest D J | 1999 | Education1999，，119： | 1 |
| 8 | The 2007 WHO clas- sification of tumours of the central nervous system 显示文摘 | Louis D N Ohgaki H Wiest/er 0 D | 2007 | Acta Neu- ropathol2007，114，2： | 1 |
| 9 | Risk profiles of different in- jectable fillers : results from the injectablefiller safety study ( IFS Study) 显示文摘 | Zielke H Wfilber L Wiest L | 2008 | Dermatol Surg2008，34，： | 1 |
| 10 | Adolescent depression: links to academic coping and perceived autonomy support显示文摘 | Wong E H Wiest D J | 1999 | Education1999，119，4： | 1 |
| 11 | Adolescent depression:Links to academic coping and perceived autonomy support显示文摘 | D J Wiest | 1999 | Education1999，119，4： | 1 |
| 12 | Adolescent depression: Links to academic coping and perceived autonomy support 显示文摘 | Wnog E H Wiest D J | 1999 | Education1999，119，4： | 1 |
| 13 | First trimester diagnosis of placenta previa percreta by magnetic resonance imaging显示文摘 | THROP J M Jr WELLS S R WIEST H H | 1998 | Am J Obstet Gynecol1998，178，： | 1 |
| 14 | Cardiomyopathy associated with the acquired immune deficiency syndrome显示文摘 | Kaminski H J Katzman M Wiest PM | | 0，，02： | 1 |
| 15 | CT scanning: A major source of radiation exposure显示文摘 | Philip W Wiest Julie A Locken Philip H Heintz Fred.A Mettler | 2002 | Seminars in Ultrasound, CT, and MRI2002，，： | 1 |
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