|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | An Algorithm for Automatic Stitching of CR X-ray Images显示文摘 | Markus G Wilfried B Berned L | 2007 | Advances in Medical Engineering2007，，114： | 1 |
| 3 | Dose-response and threrhold effects in eytotoxicity and apoptosis显示文摘 | Roll S H Bettina G K Wilfried B | 2000 | Mutation Research2000，464，： | 1 |
| 4 | Polynomial LPVsynthesis applied to turbofan engines 显示文摘 | Wilfried G Didier H Jacques B | 2010 | Control Engi- neering Practice2010，18，9： | 1 |
| 5 | Cholesteryl ester transfer protein: at the heart of the action of lipid-modulating therapy with statins, fibrates, niacin, and cholesteryl ester transfer protein inhibitors 显示文摘 | Chapman M J Wilfried L G Guerin M | 2010 | Eur Heart J2010，31，2： | 1 |
| 6 | Model based Uncertainty in Species Range Prediction显示文摘 | Richard G Pearson Wilfried Thuiller Miguel B Arafijo | 2006 | Journal of Biogeography2006，33，10： | 1 |
| 7 | Growth factor level in platelet-rich plasma and correlations with donor age,sex,and platelet count显示文摘 | Kleis Wilfried KG Hafner G | 2002 | J Cranio Maxillofacial Surg2002，30，2： | 1 |
| 8 | Molecular characterization of fervidolysin,a subtilisin-like serine protease from the thermophilic bacterium Fervidobacterium pennivorans显示文摘 | Kluskens Leon D Wilfried G B Voorhorst Roland J | 2002 | Extremophiles2002，，6： | 1 |
| 9 | Molecular characterization of fervidolysin, a subtilisin-like serine protease from the thermophilic bacterium Fervidobacterium pennivorans显示文摘 | Leon D Kluskens Wilfried G B | 2002 | Extremophiles2002，6，： | 1 |
| 10 | Ectopic Expression of Hu- man telomerase RNA component results in increased telomerase activity and elongated telomeres in bovine blastocysts 显示文摘 | Wiebke G Wilfried B Doris H | 2012 | Biol Reprod2012，10，： | 1 |
| 11 | Glycosidically bound aroma components from sour cherry 显示文摘 | Wilfried S Schwab G Selvendran P | 1990 | Phytochemistry1990，29，2： | 1 |
| 12 | Silicon and plant disease resistance against pathogenic fungi显示文摘 | Fauteux Francois Ré mus-Borel Wilfried Menies James G | 2005 | FEMS Microbiology Letters2005，249，： | 1 |
| 13 | Model-based Uncertainty in Species Range Prediction显示文摘 | Richard G Pearson Wilfried Thuiller Miguel B Arajo | 2006 | Journal of Biogeography2006，33，10： | 1 |
| 14 | Biodiversity Conservation: Uncertainty in Predictions of Extinction Risk显示文摘 | Wilfried Thuiller Miguel B Arat]jo Richard G Pearson et al | 2004 | Nature2004，430，6995： | 1 |
| 15 | QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase显示文摘Strawberry(Fragaria×ananassa)fruits are an excellent source of L-ascorbic acid(AsA),a powerful antioxidant for plants and humans.Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality.Here,we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines‘Candonga’and‘Senga Sengana’,adapted to distinct Southern and Northern European areas.To account for environmental effects,the F1 and parental lines were grown and phenotyped in five locations across Europe(France,Germany,Italy,Poland and Spain).Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold,with significant differences among genotypes and environments.AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus(QTL)analysis.Environmentally stable QTLs for AsA content were detected in linkage group(LG)3A,LG 5A,LG 5B,LG 6B and LG 7C.Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A),and the chloroplast-located AsA transporter gene FaPHT4;4(7C)might be the underlying genetic factors for QTLs on LG 3A and 7C,respectively.We show that recessive alleles of FaGGP(3A)inherited from both parental lines increase fruit AsA content.Furthermore,expression of FaGGP(3A)was two-fold higher in lines with high AsA.Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content. | Pilar Muñoz Cristina Castillejo JoséAntonio Gómez Luis Miranda Silke Lesemann Klaus Olbricht Aurélie Petit Philippe Chartier Annika Haugeneder Johanna Trinkl Luca Mazzoni Agnieszka Masny Edward Zurawicz Freya Maria Rosemarie Ziegler Björn Usadel Wilfried Schwab Béatrice Denoyes Bruno Mezzetti Sonia Osorio JoséFSánchez-Sevilla Iraida Amaya | 2023 | Horticulture Research2023，10，3： | 1 |
| 16 | A new hybrid ocean thermal energy conversion-offshore solar pond (OTEC-OSP) design:A cost optimization approach显示文摘 | PAUL J T STRAATMAN WILFRIED G J H M VAN SARK | | 0，，6： | 1 |
| 17 | Analytic path planning algorithms for bipedal robots without a trunk显示文摘 | AMOS A WILFRIED G | 2003 | Journal of Intelligent and Robotic Systems2003，36，2： | 1 |
| 18 | Innovative technologies for strip production显示文摘 | | 1999 | MPT,1999,(8):74-861999，1999，8： | 1 |
| 19 | Intensity-modulated radiation therapy for head and neck carcinoma 显示文摘 | Vincent G Wilfried D N Avraham E | 2007 | The Oneologist2007，12，5： | 1 |
| 20 | Physical limits of hyperthermia using magnetite fine pa rtjcles显示文摘 | RUDOLF H WILFRIED A CARL G d’A | 1998 | Transactions on Magnetics1998，34，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员