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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | 卒中介入治疗培训指南:国际多学会共识文件显示文摘1背景
缺血性卒中是全球人口死亡和残疾的首要原因。很多急性大血管闭塞(emergent large vesselocclusion,ELVO)患者都会遗留长期残疾。事实上,这些颅内大动脉闭塞经常会导致大面积脑损伤,进而造成患者死亡或严重致残。 | Lavine SD Cockroft K Hoh B Bambakidis N Khalessi AA Woo H Riina H Siddiqui A Hirsch JA Chong W Rice H Wenderoth J Mitchell P Coulthard A Signh TJ Phatorous C Khangure M Klurfan P ter Brugge K Iancu D Gunnarsson T Pongpech S Rodesch G Soderman M Taylor A Krings T Orbach D Picard L Suh DC Zheng HQ Jansen O Muto M Szikora I Pierot L Brouwer P Gralla J Renowden S Andersson T Fiehler J Turjman F White P Januel AC Spelle L Kulcsar Z Chapot R Biondi A Dima S Taschner C Szajner M Krajina A Sakai N Matsumaru Y Yoshknura S Ezura M Fujinaka T Iihara K Ishii A Higashi T Hirohata M Hyodo A Ito Y Kawanishi M Kiyosue H Kobayashi E Kobayashi S Kuwayama N Matsumoto Y Miyachi S Murayama Y Nagata I Nakahara I Nemoto S Niimi Y Oishi H Satomi J Satow T Sugiu K Tanaka M Terada T Yamagami H Diaz O Lylyk P Jayaraman MV Patsalides A Gandhi CD Lee SK Abruzzo T Albani B Ansari SA Arthur AS Baxter BW Bulsara KR Chen M Almandoz JE Fraser JF Heck DV Hetts SW Hussain MS Klucznik RP Leslie-Mawzi TM Mack WJ McTaggart RA Meyers PM Mocco J Prestigiacomo CA Pride GL Rasmussen PA Starke RM Sunenshine PJ Tarr RW Frei DF Pabo M Nogueira RG Zaidat OO Jovin T Linfante I Yavagal D Liebeskind D Novakovic R Pongpech S 许岩 孙瑞 郭芮兵 | 2017 | 国际脑血管病杂志2017，25，5： | 2 |
| 3 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 4 | Origins of the slow and the ubiquitous fast solar wind显示文摘 | HABBAL S R WOO R FINESCHI S | 1997 | Astro- physical Journal Letters1997，489，1： | 1 |
| 5 | Heat and moisture transfer trough nonwoven fabrics 显示文摘 | WOO S S SHALEV Itzhak BARKER R L | 1994 | Journal of Textile Research1994，64，3： | 1 |
| 6 | Removal of reactive black 5 by zero-valent iron modified with various surfactants显示文摘 | Chatterjee S Lim S R Woo S H | 2010 | Chemical Engineering Journal2010，160，1： | 1 |
| 7 | The tunnel source(PNPN)n-MOSFET:a novel high performance transistor显示文摘 | NAGAVARAPU V JHAVERI R WOO J C S | 2008 | IEEE Transactions on Electron Devices2008，55，4： | 1 |
| 8 | Networking support for query processing in sensor networks 显示文摘 | Woo A Madden S Govindan R | 2004 | Communications of the ACM2004，47，6： | 1 |
| 9 | Moisture barrier characteristics of organoclay-epoxy nanocomposites 显示文摘 | KIM J K HU Chugang WOO R S C | 2005 | Compos Sci Teehnol2005，65，5： | 1 |
| 10 | Measurement of Atlanta aerosol size distributions: Observations of ultrafine particle events 显示文摘 | Woo K S Chen D R Pui D Y H | 2001 | Aerosol Science and Technology2001，34，1： | 1 |
| 11 | DNA dependent protein kinase acts upstream of p53 in response to DNA damage 显示文摘 | WOO R A MCLURE K G LEES-MILLER S P | 1998 | Nature1998，394，6694： | 1 |
| 12 | Estuarine influences on a continental shelf plankton community显示文摘 | TURNER R E S WOO W JITTS H R | 1979 | Science1979，206，： | 1 |
| 13 | The neuregulin-1 receptor ErbB4 controls glutamatergic synapse maturation and plasticity 显示文摘 | LI B WOO R S MEI L | 2007 | Neuron2007，54，4： | 1 |
| 14 | Inhibition of L-type Ca2+channel by mitochondrial Na +-Ca2+ exchange inhibitor CGP-37157 in rat atrial myocytes显示文摘 | Thule T Ahn J R Woo S H | | 0，，1： | 1 |
| 15 | Virus-cell fusion inhibitory activity for the polysaccharides from various Korean edible clams显示文摘 | Woo E R Kim W S Kim Y S | 2001 | Arch Pharm Res2001，24，6： | 1 |
| 16 | Effects of Mg Concentration on the Quasi-superplasticity of Coarse-grained Al-Mg Alloys显示文摘 | Woo S S Kim Y R Shin D H | 1997 | Scripta Materialia1997，37，： | 1 |
| 17 | SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa) 显示文摘 | Qiao Y L Jiang W Z Lee J H Park B S Choi M S Piao R H Woo M O Roh J H Han L Z Paek N C Seo H S Koh H J | 2009 | New Phytologist2009，185，1： | 1 |
| 18 | Quasilinear viscoelastic properties of normal articular cartilage显示文摘 | Woo S L Simon B R Kuei S C | 1980 | J Biomech Eng1980，102，2： | 1 |
| 19 | Prevention of overweight and obesity: how effective is the current public health approach显示文摘 | CHAN R S WOO J | 2010 | Int J Environ Res Public Health2010，7，3： | 1 |
| 20 | Immunoprophylactic Effect of chicken Egg Yolk Immunoglobulin(IgY)against porcine epidemic diarrhea virus(PEDV)in piglets显示文摘 | Kwon B J Woo S R | 2000 | J Vet Med Sci2000，62，： | 1 |
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