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您的检索式：作者名="Wang Bingping"
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| 1 | The study of selective primary culture and determination of a breast cancer cell line in vitro显示文摘Objective The successful establishment of a tumor cell bank is based on the premise that the target cells can be cultured by a legitimate approach.In this experiment,we used primary culture to select and detect breast cancer cells in vitro,which can provide experimental ideas and methods for the establishment of a living tumor tissue cell bank.Methods Fifty-two specimens were collected over a two-year period from people with breast cancer who needed surgical treatment in our hospital.Cells were isolated and used to establish successful cell culture.Cell activity and cell purity were measured before liquid nitrogen cryopreservation.Results(1)At the initial culture stage,cells grew with adherence.Cell multiplication could be seen after the cell medium was exchanged three times.Cell viability was above 86%,while the viability of the target cells was above 75%,as detected by hematoxylin and eosin(HE)staining.(2)The number of breast cancer cells decreased,while the number of fibroblasts increased after five rounds of passage.(3)The success rate was 73.08%,which did not include polluted cells and those that were not successfully cryopreserved.Conclusion(1)breast cancer cells could be selected from primary culture in vitro through an appropriate method.(2)Exchange of the cell medium and further cell passage improved cell multiplication.(3)The experimental results could be monitored using trypan blue and HE staining.(4)The success of breast cancer cell culture in vitro could be used as a reference for other cell culture,so as to establish a tumor tissue cell bank. | Meng Ren Huixia Xu Xiangji Lu Bingping Wang Rina Su Hao Zhang Song Jiang Fengying Gao Yanwei Gao | 2020 | Oncology and Translational Medicine2020，6，2： | 1 |
| 2 | Effects of Seasons on Embryo Transfer of Cattle in Different Climatic Zones显示文摘The paper was to evaluate the efficient seasons for embryo transfer of cattle in different climatic zones in China. Three climatic zones(mid-temperate zone,warm temperate zone,subtropical zone) were designed,and embryo transfers had been carried out in spring,summer,autumn and winter from 2009 to 2011,respectively. The total number of transplant recipient cattle was 22 208. The results showed that the best seasons for embryo transfers varied with different climatic zones. In mid-temperate zone,summer and autumn were better while summer was the best,and the rate of pregnant was 50. 67%(the number of pregnant cattle was 8 005). In warm temperate zone,spring and autumn were better while autumn was the best,and the rate of pregnant was 54. 99 %(the number of pregnant cattle was 551). In subtropical zone,spring and winter were better while winter was the best,and the rate of pregnant was 56. 94 %(the number of pregnant cattle was 328). The seasonal average temperatures and relative humidity of the best seasons in different climatic zones were concentrated on the same region. In mid-temperate zone,the mean temperature ranged between 22. 4 ℃ and 24. 2 ℃,and the mean relative humidity ranged from 44% to 55. 3%. In warm zone,the mean temperature ranges were between 14. 2 ℃ and 16. 2 ℃,and the mean relative humidity ranges were from 59. 3% to 71. 6%. In sub-tropical zone,the mean temperature ranges were between 3. 26 ℃ and 7. 73 ℃,and the mean relative humidity ranges were from 72% to 80. 6%. Therefore,the optimized conditions of temperature and humidity of season in different zones could be simulated. It is possible that we apply the program to bovine production in the similar agroecological zones,which is of great significance for improving the embryo transfer efficiency of livestock in production. | Wang Feng Wang Shenyuan Zhang Dong Han Lidong Li Lu Huang Chunhua Zhong Gang Han Jinlong Wang Bingping Liu Yiyi Liu Caiyun Pan Jing Zhao Zhichao Zhou Huanmin Zhang Li | 2014 | Animal Husbandry and Feed Science2014，6，4： | 0 |
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