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| 1 | Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring显示文摘树鼯有一种靠近的关系到首领并且在生物医学的研究比啮齿类动物有许多优点。然而,基因操作方法的缺乏妨碍了这个动物的更宽的使用。Spermatogonial 干细胞(SSC ) 成功地在文化被扩展了允许复杂基因在老鼠和老鼠编辑。这里,没有干细胞性质的损失,我们为树鼯 SSC 的长期的扩大描述一个文化系统。在我们的学习,胸腺房间抗原 1 被用来充实树鼯 SSC。定序 RNA 分析表明表明小径的 Wnt/-catenin 在无差别的 SSC 是活跃的,但是是在 SSC 区别的开始之上的 downregulated。树鼯的暴露到 recombinant Wnt3a 蛋白质的主要 SSC 在文化的起始的段落期间提高了 SSC 的幸存。当 feeder 被发现为树鼯 SSC 增长必要,树鼯 Sertoli 房间,然而并非老鼠使用胚胎的成纤维细胞,导致柔韧的房间扩大和长期的文化。扩展的树鼯 SSC 是有提高的绿色的 transfected 荧光灯表示 lentiviral 向量的蛋白质(EGFP ) 。在进消毒的成年的男树鼯睾丸的移植以后,标注 EGFP 的 SSC 能恢复精子发生并且成功地产生转基因的后代。而且,这些 SSC 对 CRISPR/Cas9-mediated 基因修正合适。在有编辑的基因的联合的树鼯 SSC 接近膨胀的一个文化系统的发展用树鼯为精确染色体操作铺平道路。 | Chao-Hui Li Lan-Zhen Yan Wen-Zan Ban Qiu TH Yong Wu Lin Wang Rui Bi Shuang Ji Yu-Hua Ma Wen-Hui Nie Long-Bao Lv Yong-Gang Yao Xu-Dong Zhao Ping Zheng | 2017 | Cell Research2017，27，2： | 22 |
| 2 | Pulmonary infection control window in treatment of severe respiratory failure of chronic obstructive pulmonary diseases: a prospective, randomized controlled, multi-centred study显示文摘Background Early withdraw from invasive mechanical ventilation (MV) followed by noninvasive MV is a new strategy for changing modes of treatment. This study was conducted to estimate the feasibility and the efficacy of early extubation and sequential noninvasive MV commenced at beginning of pulmonary infection control window in patients with exacerbated hypercapnic respiratory failure caused by chronic obstructive pulmonary diseases (COPD). Methods A prospective, randomized controlled study was conducted in eleven teaching hospitals' respiratory or medical intensive care units in China. Ninety intubated COPD patients with severe hypercapnic respiratory failure triggered by pulmonary infection (pneumonia or purulent bronchitis) were involved in the study. When the pulmonary infection had been controlled by antibiotics and comprehensive therapy, the 'pulmonary infection control window (PIC window)' has been reached. Each case was randomly assigned to study group (extubation and noninvasive MV via facial mask immediately) or control group (invasive MV was received continuously after PIC window by using conventional weaning technique).Results Study group (n=47) and control group (n=43) had similar clinical characteristics initially and at the time of PIC window. Compared with control group, study group had shorter duration of invasive MV [(6.4±4.4) days vs (11.3±6.2) days, P=0.000], lower rate of ventilator associated pneumonia (VAP) (3/47 vs 12/43, P=0.014), fewer days in ICU [(12±8) days vs (16±11) days, P=0.047] and lower hospital mortality (1/47 vs 7/43, P=0.025).Conclusions In COPD patients requiring intubation and invasive MV for hypercapnic respiratory failure, which is exacerbated by pulmonary infection, early extubation followed by noninvasive MV initiated at the start of PIC window may decrease significantly the duration of invasive MV, the risk of VAP and hospital mortality. | Wang, C Zhan, QY Cao, ZX Wei, LQ Cheng, ZZ Liu, S Zhang, JL Chen, RC Luo, Q Niu, SF Zhu, L Wu, DW Fang, BM Wu, TH Wang, CZ Liu, YN | 2005 | Chinese Medical Journal2005，，19： | 6 |
| 3 | 放射性颌骨骨坏死拔牙的危险因素及作用:国家层面的队列研究显示文摘该文旨在研究接受放疗并发生放射性颌骨骨坏死(ORNJ)的头颈癌患者的相关危险因素,尤其是接受某种药物治疗后及牙的变化情况。23,527例头颈癌患者纳入研究,Cox比例风险模型用于危险因素分析。结果:ORNJ的总发生率为3.93/100人/年,颊癌患者发生ORNJ的风险最高。 | Wang TH Liu CJ Chao TF 张凌 | 2017 | 中国口腔颌面外科杂志2017，15，3： | 4 |
| 4 | Synergistic control of sex hormones by 17β-HSD type 7: a novel target for estrogen-dependent breast cancer显示文摘17β-hydroxysteroid dehydrogenase(17β-HSD)type 1 is known as a critical target to block the final step of estrogen production in estrogen-dependent breast cancer.Recent confirmation of the role of dyhydroxytestosterone(DHT)in counteracting estrogeninduced cell growth prompted us to study the reductive 17β-HSD type 7(17β-HSD7),which activates estrone while markedly inactivatingDHT.The role ofDHTin breast cancer cell proliferation isdemonstratedby its independent suppression of cell growthin the presence of a physiological concentration of estradiol(E2).Moreover,an integral analysis of a large number of clinical samples in Oncomine datasets demonstrated the overexpression of 17β-HSD7 in breast carcinoma.Inhibition of 17β-HSD7 in breast cancer cells resulted in a lower level of E2 and a higher level of DHT,successively induced regulation of cyclinD1,p21,Bcl-2,and Bik,consequently arrested cell cycle in the G0/G1 phase,and triggered apoptosis and auto-downregulation feedback of the enzyme.Such inhibition led to significant shrinkage of xenograft tumors with decreased cancer cell density and reduced 17β-HSD7 expression.Decreased plasma E2 and elevated plasma DHT levels were also found.Thus,the dual functional 17β-HSD7 is proposed as a novel target for estrogen-dependent breast cancer by regulating the balance of E2 andDHT.Thisdemonstrates aconceptual advance on the general belief that the major role of this enzyme is in cholesterol metabolism. | Xiaoqiang Wang Catherine Gérard Jean-Francois Thériault Donald Poirier Charles J.Doillon Sheng-Xiang Lin | 2015 | Journal of Molecular Cell Biology2015，7，6： | 2 |
| 5 | Association between high-sensitivity cardiac troponin T and N-terminal pro-brain natriuretic peptide in a community based population显示文摘 | Xu RY Ye P Luo LM Sheng L Wu HM Xiao WK Zheng J Wang F Xiao TH | 2014 | Chinese Medical Journal2014，，4： | 2 |
| 6 | Aspirin and clopidogrel resistance : an emerging clinical entity显示文摘 | Wang TH Bhatt DL Topoi EJ | 2006 | Eur Heart J2006，27，： | 1 |
| 7 | Angiotensin II stimulates hypoxia -inducible factor let accumulation in glomerular mesangial cells 显示文摘 | Chen TH Wang JF Chan P | 2005 | Ann N Y Acad Sci2005，1042，1： | 1 |
| 8 | An aluminium heat switch made from cold-pressed Cu/A1 composite显示文摘 | Wang H L Wagner Th Eska G | 2000 | Physica B2000，28，4288： | 1 |
| 9 | Cigarette smoking and cognitive impairment: a 10-year cohort study in Taiwan显示文摘 | Wang CC Lu TH Liao WC | 2010 | Arch Gerontol Geriatr2010，51，2： | 1 |
| 10 | Single injection of naked plasmid encoding alpha-melanocyte- stimulating hormone protects against thioacetamide-induced acute liver failure in mice 显示文摘 | WANG CH JAWAN B LEE TH | 2004 | Biochem Biophys Res Commun2004，322，1： | 1 |
| 11 | IMP-4 and OXA beta-lactamases in Acinetobacter baumannii from Singapore显示文摘 | Koh TH Sng LH Wang GC | 2007 | J Antimicrob Chemother2007，59，4： | 1 |
| 12 | Mechanisms of 17beta-estradiol on the production of ET-1 in ovariectomized rats显示文摘 | TAN Z WANG TH YANG D | 2003 | Life Sci2003，73，21： | 1 |
| 13 | Assays for the assessment of neutralizing antibody activities against severe acute respiratory syndrome ( SARS ) associated coronavirus ( SCV ) 显示文摘 | Wang S Sakhatskyy P Chou TH | 2005 | J Immunol Methods2005，301，12： | 1 |
| 14 | Dosimetric verification of inten sity-modulated fields显示文摘 | Wang X Spirou S LoSasso TH | 1996 | Med Phys1996，23，： | 1 |
| 15 | General cone-beam reconstruction algorithm显示文摘 | Wang G Lin TH Cheng PC | 1993 | IEEE Transactions on Medical Imaging1993，12，3： | 1 |
| 16 | Immunobiology of hepatitis C virus (HCV) infection: the role of CD4 T cells in HCV infection显示文摘 | Eckels DD Wang H Bian TH | 2000 | Immunol Rev2000，174，1： | 1 |
| 17 | Carcinoma of the lung with metastases to skeletal muscles 显示文摘 | CHEONG TH WANG YT POH SC THUNG JL | 1989 | Singapore Med J1989，30，6： | 1 |
| 18 | Farfield impact of water injection dredging in the Crouch River 显示文摘 | Winterwerp J C Wang Z B Kester J A Th M van | 2002 | Water & maritime engineering2002，，4： | 1 |
| 19 | Molecular cloning and char- acterization of interferon regulatory factors 4 and 8 ( IRF4 and 1RF8 ) in rainbow trout, Om:orhynchus mykiss 显示文摘 | Holland JW Karim A Wang TH | 2010 | Fish and Shellfmh Immunology2010，29，1： | 1 |
| 20 | Clinical and radiographicpresentions of pulmonary cryptococcosis in immunocompetentpatients显示文摘 | Yang C J Hwang JJ Wang TH | 2006 | Scand J Infect Dis2006，38，9： | 1 |
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