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| 1 | A New Method of Wind Turbine Bearing Fault Diagnosis Based on Multi-Masking Empirical Mode Decomposition and Fuzzy C-Means Clustering显示文摘Based on Multi-Masking Empirical Mode Decomposition (MMEMD) and fuzzy c-means (FCM) clustering, a new method of wind turbine bearing fault diagnosis FCM-MMEMD is proposed, which can determine the fault accurately and timely. First, FCM clustering is employed to classify the data into different clusters, which helps to estimate whether there is a fault and how many fault types there are. If fault signals exist, the fault vibration signals are then demodulated and decomposed into different frequency bands by MMEMD in order to be analyzed further. In order to overcome the mode mixing defect of empirical mode decomposition (EMD), a novel method called MMEMD is proposed. It is an improvement to masking empirical mode decomposition (MEMD). By adding multi-masking signals to the signals to be decomposed in different levels, it can restrain low-frequency components from mixing in highfrequency components effectively in the sifting process and then suppress the mode mixing. It has the advantages of easy implementation and strong ability of suppressing modal mixing. The fault type is determined by Hilbert envelope finally. The results of simulation signal decomposition showed the high performance of MMEMD. Experiments of bearing fault diagnosis in wind turbine bearing fault diagnosis proved the validity and high accuracy of the new method. | Yongtao Hu Shuqing Zhang Anqi Jiang Liguo Zhang Wanlu Jiang Junfeng Li | 2019 | Chinese Journal of Mechanical Engineering2019，32，3： | 6 |
| 2 | Classification of four distinct osteoarthritis subtypes with a knee joint tissue transcriptome atlas显示文摘The limited molecular classifications and disease signatures of osteoarthritis(OA)impede the development of prediagnosis and targeted therapeutics for OA patients.To classify and understand the subtypes of OA,we collected three types of tissue including cartilage,subchondral bone,and synovium from multiple clinical centers and constructed an extensive transcriptome atlas of OA patients.By applying unsupervised clustering analysis to the cartilage transcriptome,OA patients were classified into four subtypes with distinct molecular signatures:a glycosaminoglycan metabolic disorder subtype(C1),a collagen metabolic disorder subtype(C2),an activated sensory neuron subtype(C3),and an inflammation subtype(C4).Through ligand-receptor crosstalk analysis of the three knee tissue types,we linked molecular functions with the clinical symptoms of different OA subtypes.For example,the Gene Ontology functional term of vasculature development was enriched in the subchondral bone-cartilage crosstalk of C2 and the cartilage-subchondral bone crosstalk of C4,which might lead to severe osteophytes in C2 patients and apparent joint space narrowing in C4 patients.Based on the marker genes of the four OA subtypes identified in this study,we modeled OA subtypes with two independent published RNA-seq datasets through random forest classification.The findings of this work contradicted traditional OA diagnosis by medical imaging and revealed distinct molecular subtypes in knee OA patients,which may allow for precise diagnosis and treatment of OA. | Chunhui Yuan Zongyou Pan Kun Zhao Jun Li Zixuan Sheng Xudong Yao Hua Liu Xiaolei Zhang Yang Yang Dongsheng Yu Yu Zhang Yuzi Xu Zhi-Yong Zhang Tianlong Huang Wanlu Liu Hongwei Ouyang | 2020 | Bone Research2020，8，4： | 4 |
| 3 | Human health risk assessment of heavy metals in soil–vegetable system: A multi-medium analysis显示文摘 | Xingmei Liu Qiujin Song Yu Tang Wanlu Li Jianming Xu Jianjun Wu Fan Wang Philip Charles Brookes | 2013 | Science of the Total Environment2013，，： | 4 |
| 4 | Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation显示文摘Fundamental processes such as ribosomal RNA synthesis and chromatin remodeling take place in the nucleolus,which is hyperactive in fast-proliferating cells.The sophisticated regulatory mechanism underlying the dynamic nucleolar structure and functions is yet to be fully explored.The present study uncovers the mutual functional dependency between a previously uncharacterized human long non-coding RNA,which we renamed LETN,and a key nucleolar protein,NPM1.Specifically,being upregulated in multiple types of cancer,LETN resides in the nucleolus via direct binding with NPM1.LETN plays a critical role in facilitating the formation of NPM1 pentamers,which are essential building blocks of the nucleolar granular component and control the nucleolar functions.Repression of LETN or NPM1 led to similar and profound changes of the nucleolar morphology and arrest of the nucleolar functions,which led to proliferation inhibition of human cancer cells and neural progenitor cells.Interestingly,this inter-dependency between LETN and NPM1 is associated with the evolutionarily new variations of NPM1 and the coincidental emergence of LETN in higher primates.We propose that this human-specific protein–lncRNA axis renders an additional yet critical layer of regulation with high physiological relevance in both cancerous and normal developmental processes that require hyperactive nucleoli. | Xianteng Wang Xiaolin Hu Wanlu Song Hui Xu Zhengtao Xiao Rongyao Huang Qingran Bai Fan Zhang Yongzhen Chen Yu Liu Jianhuo Fang Xin Li Qin Shen Haitao Zhao Xuerui Yang | 2021 | Cell Research2021，31，6： | 3 |
| 5 | Investigation of carbonyl amidation and O-methylation during biosynthesis of the pharmacophore pyridyl of antitumor piericidins显示文摘Piericidins are a large family of bacterialα-pyridone antibiotics with antitumor activities such as their anti-renal carcinoma activity exhibited recently in nude mice.The backbones of piericidins are derived fromβ,δ-diketo carboxylic acids,which are offloaded from a modular polyketide synthase(PKS)and putatively undergo a carbonyl amidation beforeα-pyridone ring formation.The tailoring modifications to theα-pyridone structure mainly include the verified hydroxylation and O-methylation of the C-4′position and an unidentified C-5′O-methylation.Here,we describe a piericidin producer,terrestrial Streptomyces conglobatus,which contains a piericidin biosynthetic gene cluster in two different loci.Deletion of the amidotransferase gene pieD resulted in the accumulation of two fatty acids that should be degraded from the nascent carboxylic acid released by the PKS,supporting the carbonyl amidation function of PieD duringα-pyridone ring formation.Deletion of the O-methyltransferase gene pieB1 led to the production of three piericidin analogues lacking C-5′O-methylation,therefore confirming that PieB1 specifically catalyses the tailoring modification.Moreover,bioactivity analysis of the mutant-derived products provided clues regarding the structure-function relationship for antitumor activity.The work addresses two previously unidentified steps involved in pyridyl pharmacophore formation during piericidin biosynthesis,facilitating the rational bioengineering of the biosynthetic pathway towards valuable antitumor agents. | Wanlu Li Wenyu Zhang Yijia Cheng Yaoyao Shen Jianzhao Qi Hou-Wen Lin Yongjun Zhou | 2022 | Synthetic and Systems Biotechnology2022，7，3： | 2 |
| 6 | Piezoresistive effect in carbonnanotube films显示文摘The piezoresistive effect of the pristine carbon nanotube (CNT) films has been studied. Carbon nanotubes were synthesized by hot filament chemical vapor deposition. The piezoresistive effect in the pristine CNT films was stud-ied by a three-point bending test. The gauge factor for the pristine CNT films under 500 microstrains was found to be at least 65 at room temperature, and increased with tem-perature, exceeding that of polycrystalline silicon (30) at 35℃. The origin of the piezoresistivity in CNT films may be ascribed to a pressure-induced change in the band gap and the defects. | LI Yong, WANG Wanlu, LIAO Kejun, HU Chenguo, HUANG Zhi & FENG Qing Department of Applied Physics, Chongqing University, Chongqing 400044, China Correspondence should be addressed to Wang Wanlu (e-mail: wanluw@ cqu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，2： | 2 |
| 7 | Human health risk assessment of heavy metals in soil–vegetable system: A multi-medium analysis显示文摘 | Xingmei Liu Qiujin Song Yu Tang Wanlu Li Jianming Xu Jianjun Wu Fan Wang Philip Charles Brookes | 2013 | Science of the Total Environment2013，，： | 1 |
| 8 | Research Progress on Contamination Status,Transmission and Treatment Technology of Antibiotics in Water Environment显示文摘With the wide application of antibiotics in medicine and industry fields as well as the improvement of water quality detection technology,more and more reports show that antibiotics and antibiotic resistance genes(AGRs)were detected in various drinking water sources.The existence of these pollutants in water environment posed a great threat to human health.In this paper,the pollution status of antibiotics and transmission routes of antibiotic genes were introduced,and the characteristics and shortcomings of antibiotic treatment technology both at home and abroad were analyzed.Finally,suggestions and prospects for future antibiotic treatment technology were put forward. | Wanlu REN Gaoping XU Dong LI | 2022 | Meteorological and Environmental Research2022，13，1： | 1 |
| 9 | Superwettable interface towards biodetection in confined space显示文摘The detection of biomarkers with both high sensitivity and specificity is crucial for the diagnosis and treatment of related diseases.However,many current detections employ ex-situ detection method and non-confined condition,thus have many problems,which may eventually lead to inaccurate detection results.Compared to detection in non-confined space,detection in confined space can better reflect the real in-vivo situation.Therefore,the construction of detection for target molecules in confined space has great significance for both theoretical research and practical application.To realize the detection of target molecules in confined space,the probes should accurately enter the confined space where the target molecules reside and interact with the interface.Thus,how to explore and utilize the properties of the interface(for example,bioinspired superwettability)has always been a hot and difficult topic in this field.Herein,the recent advances and our efforts in recent 10 years on detection of bio-target molecules in confined space with superwettable interface have been introduced from the perspective of the detection methods.The suitable and most widely employed detection methods for target molecules in confined spaces are introduced firstly.Then,recent progresses for related detections based on visual,optical,and electrochemical detection methods are presented successively.Finally,the perspective for detection in confined space is discussed for the future development of biochemical detection. | Zexu Pang Zhikang Cao Wanlu Li Wenxia Xu Yingying Zhang Qitao Zhou Jing Pan Fan Xia | 2024 | Nano Research2024，17，2： | 1 |
| 10 | Piezoresistive effect of doped carbon nanotubes/cellulose films 显示文摘 | Liao Kejun Li Yong | 2003 | Chin Phys Lett2003，20，9： | 1 |
| 11 | The identification of 'hotspots' of heavy metal polltion in soil- rice systems at a regional scale in eastern China 显示文摘 | Li Wanlu Xu Binbin Song Qiujin | 2014 | Science of the Total Environment2014，472，： | 1 |
| 12 | Synthesis of reduced graphene nanosheet/urchin-like manganese dioxide composite and high performance as supercapacitor electrode显示文摘 | Wanlu Yang Zan Gao Jun Wang Bin Wang Qi Liu Zhanshuang Li Tom Mann Piaoping Yang Milin Zhang Lianhe Liu | 2012 | Electrochimica Acta2012，，： | 1 |
| 13 | Periplasmic expression optimization of VEGFR2 D3 adopting response surface methodology: antiangiogenic activity study 显示文摘 | CAO Wanlu LI Halxin ZHANG Juan | 2013 | Pro- tein Expr Purif2013，90，2： | 1 |
| 14 | Combination of bio-and chemocatalysis for dynamic kinetic resolution:The assembly strategies for nanozeolite-modified flow microchannel reactors显示文摘 | Wang Zhoujun Li Xiang Wang Wanlu | | 0，，： | 1 |
| 15 | 3D-bioprinted cholangiocarcinoma-on-a-chip model for evaluating drug responses显示文摘Cholangiocarcinoma(CCA)is characterized by heterogeneous mutations and a refractory nature.Thus,the development of a model for effective drug screening is urgently needed.As the established therapeutic testing models for CCA are often ineffective,we fabricated an enabling three-dimensional(3D)-bioprinted CCA-on-a-chip model that to a good extent resembled the multicellular microenvironment and the anatomical microstructure of the hepato-vascular-biliary system to perform high-content antitumor drug screening.Specifically,cholangiocytes,hepatocytes,and vascular endotheliocytes were employed for 3D bioprinting of the models,allowing for a high degree of spatial and tube-like microstructural control.Interestingly,it was possible to observe CCA cells attached to the surfaces of the gelatin methacryloyl(GelMA)hydrogelembedded microchannels and overgrown in a thickening manner,generating bile duct stenosis,which was expected to be analogous to the in vivo configuration.Over 4000 differentially expressed genes were detected in the CCA cells in our 3D coculture model compared to the traditional two-dimensional(2D)monoculture.Further screening revealed that the CCA cells grown in the 3D traditional model were more sensitive to the antitumoral prodrug than those in the 2D monoculture due to drug biotransformation by the neighboring functional hepatocytes.This study provides proof-of-concept validation of our bioprinted CCA-on-a-chip as a promising drug screening model for CCA treatment and paves the way for potential personalized medicine strategies for CCA patients in the future. | Qiong Liu Luis SMille Cesar Villalobos Ingrid Anaya Matthias Vostatek Sili Yi Wanlu Li Junlong Liao Huanghui Wu Yongteng Song Lize Xiong Yu Shrike Zhang | 2023 | Bio-Design and Manufacturing2023，6，4： | 0 |
| 16 | Digital light processing (DLP)-based (bio)printing strategies for tissue modeling and regeneration显示文摘Digital light processing(DLP)-based bioprinting technology has recently aroused considerable concerns as a strategy to deliver biomedical materials and/or specific cells to create sophisticated structures for various tissue modeling and regeneration.In this review,we display a concise introduction of DLP bioprinting,and a further discussion on the design and manufacture of DLP(bio)printer with varied bioinks and their biomedical applications toward drug screening,disease modeling,tissue repair,and regenerative medicine.Finally,the advantages,challenges,and perspectives of the DLP printing platforms are detailed.It is believed that DLP bioprinting will play a decisive role in the field of tissue model and regenerative medicine,mainly due to its time-efficient,higher resolution,and amenability to automation for various tissue needs. | Hongbin Li Jiliang Dai Zixuan Wang Heshan Zheng Wanlu Li Mian Wang Feng Cheng | 2023 | Aggregate2023，4，2： | 0 |
| 17 | Biosynthesis of trialkyl-substituted aromatic polyketide NFAT-133 involves unusual P450 monooxygenase-mediating aromatization and a putative metallo-beta-lactamase fold hydrolase显示文摘The bacterial trialkyl-substituted aromatic polyketides are structurally featured with the unusual aromatic core in the middle of polyketide chain such as TM-123(1),veramycin A(2),NFAT-133(3)and benwamycin I(4),which were discovered from Streptomyces species and demonstrated with antidiabetic and immunosuppressant activities.Though the biosynthetic pathway of 1-3 was reported as a type I polyketide synthase(PKS),the PKS assembly line was interpreted inconsistently,and it remains a mystery how the compound 3 was generated.Herein,the PKS assembly logic of 1-4 was revised by site-mutagenetic analysis of the PKS dehydratase domains.Based on gene deletion and complementation,the putative P450 monooxygenase nftE1 and metallo-beta-lactamase(MBL)fold hydrolase nftF1 were verified as essential genes for the biosynthesis of 1-4.The absence of nftE1 led to abolishment of 1-4 and accumulation of new products(5-8).Structural elucidation reveals 5-8 as the non-aromatic analogs of 1,suggesting the NftE1-catalyzed aromatic core formation.Deletion of nftF1 resulted in disappearance of 3 and 4 with the compounds 1 and 2 unaffected.As a rare MBL-fold hydrolase from type I PKSs,NftF1 potentially generates the compound 3 through two strategies:catalyze premature chain-offloading as a trans-acting thioesterase or hydrolyze the lactone-bond of compound 1 as an esterase. | Ming Yang Wanlu Li Lin Zhou Xiao Lin Wenyu Zhang Yaoyao Shen Hai Deng Hou-wen Lin Yongjun Zhou | 2023 | Synthetic and Systems Biotechnology2023，8，3： | 0 |
| 18 | Inorganic matter in rice husk derived carbon and its effect on the capacitive performance显示文摘Porous carbons were obtained from rice husk using two different chemical activation methods and they were investigated as supercapacitors.Their properties were studied using X-ray photoelectron spectroscopy,thermal analysis,potentiometric titration,and nitrogen adsorption isotherm.The specific capacitance measured in both H_(2) SO_(4) and KOH electrolytes in two-electrode cell was up to-150 F/g.The activation method used affected the resulting carbons’features.As expected,the dependence of the capacitance on porosity was found.The ash content reached 36 wt.%and that inorganic mater blocked some pores and limited their accessibility to electrolyte ions and increased the charge transfer resistance.Nevertheless,the main ash constituents such as CaCO_(3),MgCO_(3),Ca_(3)(PO_(4))2(or P_(2)O_(5)),and Fe-and Zn-containing species did not affect the specific capacitance to a large extent.Especially SiO2,even in a relatively large amount(~20 wt.%)T did not play a detrimental role in the capacitance behavior.The results showed that in spite of a high ash content,carbon can exhibit a good capacitive performance provided that it has a favorable porosity and is rich in sp_(2) configurations. | Wanlu Li Meruyert Nazhipkyzy Teresa J.Bandosz | 2021 | Journal of Energy Chemistry2021，30，6： | 0 |
| 19 | Propofol inhibits cells migration and invasion via HOTAIR/miR-93/HIF-1α-mediated lactate secretion in colon cancer显示文摘Propofol,a common intravenous anesthetic used in clinical,has been shown to regulate cells proliferation,inflammation,angiogenesis and metastasis as well as exerting anti-cancer effect in several different types of cancer.However,the functions and mechanisms of propofol in lactate secretion and invasion of cancer cells remain unknown.The primary aim of this study was to investigate the role of propofol in cells migration and invasion of colon cancer(CRC).Scratches assay,Transwell assay were used to detect colon cells migration and invasion.Realtime Quantitative Polymerase Chain Reaction(RT-qPCR),Western blotting were utilized to detect the expression of related molecules.Dual-luciferase assay was performed to identify transcriptional activity of target genes,RNA pulldown assay was used to confirm the interactions of RNAs.Results indicated that propofol inhibited cell migration and invasion through suppressing lactic acid production in CRC.Moreover,propofol reduced lactic acid production by down-regulating the expression of hypoxia inducible factor-1α(HIF-1α)and monocarboxylate transporter 4(MCT4).In addition,propofol inhibited the expression of HIF-1αand cells migration/invasion via suppressing the interaction of HOX transcript antisense RNA(HOTAIR)with miR-93.The present study indicated that propofol inhibited colon cancer cell migration and invasion by reducing lactic acid production and the expression of HIF-1αand MCT4 via HOTAIR/miR-93 axis.These data may provide potential metabolic targets for CRC treatment. | RUONAN GU WENJING GUO WENYANG WANG GUIHUAN LI XIAOJU LAI ZHIBIN HUANG WANLU ZHAO ZHICONGWU HUA CHEN WENYANG LUO FANGYIN ZENG FAN DENG | 2021 | BIOCELL2021，45，6： | 0 |
| 20 | In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy显示文摘Advances in biomimetic three-dimensional(3D) melanoma models have brought new prospects of drug screening and disease modeling, since their physiological relevancy for recapitulating in vivo tumor architectures is more accurate than traditional two-dimensional(2D) cell culture. Gelatin methacryloyl(GelMA) is widely used as a tissue-engineered scaffold hydrogel for 3D cell culture. In the present study, an in vitro 3D malignant melanoma model based on Gel MA was fabricated to evaluate the efficiency of hypericin(Hy)-loaded microemulsion(ME) in photodynamic therapy against melanoma. The ME was produced by the spontaneous emulsification method to enhance the bioavailability of Hy at tumor sites. Hy-loaded MEs were applied to a 3D malignant melanoma model made using 6% Gel MA and the co-culture of B16F10 and Balb/c 3T3 cells,followed by crosslinking using violet light(403 nm). The observation revealed excellent cell viability and the presence of F-actin cytoskeleton network. Hy-loaded MEs exhibited higher phototoxicity and cell accumulation(about threefold) than free Hy, and the cells cultured in the 3D system displayed lower susceptibility(about 2.5-fold) than those in 2D culture.These findings indicate that the developed MEs are potential delivery carriers for Hy;furthermore, Gel MA hydrogel-based modeling in polydimethylsiloxane(PDMS) molds is a user-friendly and cost-effective in vitro platform to investigate drug penetration and provide a basis for evaluating nanocarrier efficiency for skin cancer and other skin-related diseases. | Hui LMa Wanlu Li Mian Wang Laudemir C.Varanda Janice R.Perussi Y.Shrike Zhang Emanuel Carrilho | 2022 | Bio-Design and Manufacturing2022，5，4： | 0 |
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