|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution显示文摘Tea green leafhopper(TGL),Empoasca onukii,is of biological and economic interest.Despite numerous studies,the mechanisms underlying its adaptation and evolution remain enigmatic.Here,we use previously untapped genome and population genetics approaches to examine how the pest adapted to different environmental variables and thus has expanded geographically.We complete a chromosome-level assembly and annotation of the E.onukii genome,showing notable expansions of gene families associated with adaptation to chemoreception and detoxification.Genomic signals indicating balancing selection highlight metabolic pathways involved in adaptation to a wide range of tea varieties grown across ecologically diverse regions.Patterns of genetic variations among 54 E.onukii samples unveil the population structure and evolutionary history across different tea-growing regions in China.Our results demonstrate that the genomic changes in key pathways,including those linked to metabolism,circadian rhythms,and immune system functions,may underlie the successful spread and adaptation of E.onukii.This work highlights the genetic and molecular basis underlying the evolutionary success of a species with broad economic impacts,and provides insights into insect adaptation to host plants,which will ultimately facilitate more sustainable pest management. | Qian Zhao Longqing Shi Weiyi He Jinyu Li Shijun You Shuai Chen Jing Lin Yibin Wang Liwen Zhang Guang Yang Liette Vasseur Minsheng You | 2022 | Genomics, Proteomics & Bioinformatics2022，20，6： | 1 |
| 2 | A stochastic approach to global optimization of nonlinear programming problem with many equality constraints显示文摘 | Feng Enmin Qian Weiyi | 2004 | Applied Mathematics and Computation2004，155，： | 1 |
| 3 | Anew methodwithsufficientsescentpropertyforunconstrainedoptimization显示文摘 | Qian Weiyi CuiHaijuan | | AbstrApplAnal0，，： | 1 |
| 4 | Adaption differential evalution algorithm for multiobjective optimization problems显示文摘 | QIAN Weiyi LI Ajun | 2008 | Applied Mathematics and Computation2008，201，： | 1 |
| 5 | Mechanoresponsive MiR-138-5p Targets MACF1 to Inhibit Bone Formation显示文摘Mechanical stimuli play an essential role in maintaining bone remodeling and skeletal integrity.Meanwhile,bone can respond to the changes of mechanical condition to adjust its mass and architecture.Clinical studies discover that bedridden patients showed osteoporotic T-scores and low bone mineral density,and long-term immobilized patients presented reduced markers of bone formation.However,as bone formation mediated by osteoblast differentiation is a complex process,the underlying molecular mechanism of mechanical stimuli regulating bone formation is still unclear.Recent evidences show that microRNAs(miRNAs)are involved in mechanical stimuli regulating bone formation or osteoblast differentiation.Nevertheless,no direct evidence identifies mechanoresponsive miRNA in both human and animal bones,and clarifies its mechanoresponsive role under different mechanical conditions(e.g.mechanical unloading,reloading,loading).In the current study,we screened for differentially expressed miRNAs in bone specimens of bedridden patients with fractures,then identified that the expression of miR-138-5p,but not the other miRNAs,altered withbedridden time and was negatively correlated with the expression of the bone formation marker genes Alp(alkaline phosphatase).Moreover,miR-138-5p was up-regulated with reduced bone formation during unloading and down-regulated with increased bone formation during reloading in hind4imb unloaded mice.In addition,miR-138-5p was verified to be responsive to different mechanical unloading condition and cyclic mechanical stretch condition in primary osteogenic cells,respectively.Further in vitro data suggested that mechanoresponsive miR-138-5p directly targeted microtubule actin crosslinking factor 1(MACF1)to inhibit osteoblast differentiation.In vivo,we constructed an osteoblastic miR-138-5p transgenic mice model(TG138)with the Runx2promoter,and found that overexpression miR-138-5p supressed bone formation.Moreover,osteoblast-targeted inhibition of miR-138-5p sensitized bone anabolic response to mechanical loading in TG138 mice.Predominantly,the osteoblast-targeted inhibition of miR-138-5p could counteract bone formation reduction induced by hind limb unloading.Taken together,the mechanoresponsive miR-138-5p inhibited bone anabolic response for developing a novel bone anabolic sensitization strategy. | Zhihao Chen Zhao Fan Liang Chao Lifang Hu Chen Lei Zhang Yan Yin Chong Dijie Li Tian Ye Wuxia Qiu Kewen Zhang Chaofei Yang Xiaona Li Li Yu Weiyi Chen Zhang Ge Qian Airong | 2019 | 医用生物力学2019，34，A01： | 1 |
| 6 | Broadly neutralizing antibodies derived from the earliest COVID-19 convalescents protect mice from SARS-CoV-2 variants challenge显示文摘Coronavirus disease 2019(COviD-19)was first reported three years ago,when a group of individuals were infected with the original SARS-CoV-2 strain,based on which vaccines were developed.Here,we develop six human monoclonal antibodies(mAbs)from two elite convalescents in Wuhan and show that these mAbs recognize diverse epitopes on the receptor binding domain(RBD)and can inhibit the infection of SARS-CoV-2 original strain and variants of concern(VOCs)to varying degrees,including Omicron strains XBB and XBB. | Qianyun Liu Haiyan Zhao Zhiqiang Li Zhen Zhang Rui Huang Mengxue Gu Ke Zhuang Qing Xiong Xianying Chen Weiyi Yu Shengnan Qian Yuzhen Zhang Xue Tan Muyi Zhang Feiyang Yu Ming Guo Zhixiang Huang Xin Wang Wenjie Xiang Bihao Wu Fanghua Mei Kun Cai Limin Zhou Li Zhou Ying Wu Huan Yan heng Cao Ke Lan Yu Chen | 2023 | Signal Transduction and Targeted Therapy2023，8，10： | 0 |
| 7 | Ultrasound Tweezers Trigger Single-Cell Mechanical Dynamics for Cell Mechanophenotyping显示文摘Cells actively modulate mechanobiological circuitry against external perturbations to stabilize whole cell/tissue physiology.The dynamic adaption of cells to mechanical force is critical for cells to perform vital biological functions,from single cell migration to embryonic development.Dysregulation of such dynamics has been associated with pathophysiological conditions in cardiovascular diseases,cancer,aging,and developmental disorders[1].Therefore,a direct understanding of cell’s biomechanical adaptive/maladaptive behaviors and the trigger factors causing the transformation of healthy adaption to maladaptation can help reveal the regulatory role of single cell mechanosensitive dynamics in the progression of various degenerative diseases and aging.However,current efforts for uncovering fundamental associations between disease and cell architecture have been focusing on'static'measurements of biophysical properties,which is limited by the requirement of large sample sizes to obtain statistically significant data.We therefore developed a single and highly integrated platform with mechanical stimulation and fine spatiotemporal sensing functions to probe the single cell mechanical dynamics at subcellular level to determine cell’s mechanophenotypes in healthy and disease conditions.We developed an integrated micromechanical system composed of an’ultrasound tweezer’stimulator[2]and a PDMS micropillar array [3] cellular force sensor to in situ noninvasively probe and monitor single cell mechanical dynamics.Vascular smooth muscle cells(VSMCs)from healthy mouse and mouse with induced abdominal aorta aneurysm(AAA)were used for cell mechanobiological study.An ultrasound transducer(V312-SM,Olympus)was used to generate ultrasound pulses to excite lipid-encapsulated microbubbles(Targeson)binding to cell membrane through an RGD-integrin linkage to apply a transient nanonewton force to VSMCs seeded on the PDMS micropillar array.PDMS micropillar array was fabricated and functionalized as previously described [3] and acts as the mechanical force sensor in our platform.Upon a 1 HZ and 10-second ultrasound stimulation,calcium influx was clearly detected in both healthy and AAA-VSMCs by using the fluo-4 calcium sensor,suggesting the microbubble-integrin-actin cytoskeleton(CSK)linkage can serve as a mechanosensory to sense the ultrasound stimulation.We then examined how healthy and AAA VSMCs would exhibit adaptions to mechanical stimulation at a global cellular scale.After the onset of a 10-second ultrasound stimulation,control and AAA-VSMCs displayed distinct dynamics of CSK tension within 30 mins,in which the CSK tension of healthy VSMCs increased within the reinforcement period(0-5 min)and restored to their ground state with the relaxation period(5-10 min);yet AAA-VSMCs displayed compromised dynamics of such CSK tension upon calcium influx.Quantitative analysis and theoretical modelling revealed the critical roles of myosin motor contraction,F-actin filament polymerization in regulating cell mechanosensitive dynamics in response to a transient mechanical perturbation.The distinct force and CSK dynamics in healthy and AAA conditions indicates that the force-dependent CSK molecular kinetics is a critical factor governing the distinct mechanosensitive dynamics of cells under pathologically dysfunctional conditions.Our results reveal that the mechanical adaptive process of cells to mechanical stimulus can measure the cellular mechanobiological phenotypes featured in both pathologically healthy and diseased context.We demonstrated that an altered mechanobiological phenotype,i.e.AAA-VSMCs with distinct actomyosin-CSK properties potentiates a mechanical maladaptation that reflects progressive accumulation of cellular damage and dysfunction.This may further reveal the pathogenic contexts and their physical mediators featuring biophysical dysregulation in cardiovascular diseases. | Weiyi Qian Apratim Bajpai Jie Tong Zijing Zhang Weiqiang Chen | 2019 | 医用生物力学2019，34，A01： | 0 |
| 8 | Rh-Catalyzed Reaction of Propargylic Alcohols with Aryl Boronic Acids-Switch from β-OH Elimination to Protodemetalation显示文摘Summary of main observation and conclusion It is known that Rh-catalyzed reaction of propargyic alcohols with aryl metallic reagents undergoes Su2Z-type reaction affording alenes via a sequential arylmetalation andβ-OH elimination process.Here we report a Rh/Ag cocatalyed reaction of pro-pargylic alcohols with organoboronic acids affording stereo defined(E)-3-arlallylic alcohols via arylmetalation and protodemetalation with a high regio-and stereoselectivity under very mild conditions.The reaction exhibits a good substrate scope and the compatibility with synthetically useful functional groups with no racemization for optically active propargylic alcohols.Such a reaction may also be extended to homopropargylie alcohols with a remarka-ble regioselectivity and exclusive E-stereoselectity. | Weiyi Wang Hui Qian Shengming Ma | 2020 | Chinese Journal of Chemistry2020，38，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员