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| 1 | Development of a high-throughput assay for the HIV-1 integrase disintegration reaction显示文摘Both HIV-1 integrase (IN) and the central catalytic domain of IN (IN-CCD) catalyze the disintegration reaction in vitro.In this study,IN and IN-CCD proteins were expressed and purified,and a high-throughput format enzyme-linked immunosorbent assay (ELISA) was developed for the disintegration reaction.IN exhibited a marked preference for Mn2+ over Mg2+ as the divalent cation cofactor in disintegration.Baicalein,a known IN inhibitor,was found to be an IN-CCD inhibitor.The assay is sensitive and specific for the study of disintegration reaction as well as for the in vitro identification of antiviral drugs targeting IN,especially targeting IN-CCD. | HE HongQiu,LIU Bin,ZHANG XiaoYi,CHEN WeiZu & WANG CunXin College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China | 2010 | Science China(Life Sciences)2010，53，2： | 7 |
| 2 | Geochemical Information Indicating the Water Recharge to Lakes and Immovable Megadunes in the Badain Jaran Desert显示文摘Calc-sinters distributed in the middle of lakes and rhizoconcretions scattered at the slopes of sand dunes were observed during three explorations to the Badain Jaran Desert in the past two years. Wet sands were also found underneath the dry surface sand layers of about 20–50 cm in thickness. The geochemical parameters were measured on minerals and water samples collected from the Badain Jaran Desert and neighboring areas. The results show that the water system in the desert may be recharged from the groundwater originating from the precipitation of the Qilian Mountains and/or the Tibetan Plateau rather than the local rainfalls. | CHEN Jiansheng ZHAO Xia SHENG Xuefeng WANG Jiyang GU Weizu CHEN Liang | 2005 | Acta Geologica Sinica(English Edition)2005，79，4： | 6 |
| 3 | 粗集料对水泥混凝土路面使用寿命的影响显示文摘在水泥混凝土路面高等级公路大发展的时期,认识影响其使用寿命的主要因素并在建设过程中给予充分重视是十分必要的。本文着重说明粗集料对混凝土路面使用寿命的重要作用。 | Qin Weizu(Tsinghua University,Beijing) | 1998 | 公路交通科技1998，15，A01： | 5 |
| 4 | Network analysis of protein-protein interaction显示文摘Residue networks are constructed by defining the residues as the vertices and atom contacts between them as the edges. The residue network of a protein complex is divided into two types of networks, i.e. the hydrophobic and the hydrophilic residue networks. By analyzing the network parameters, it is found that the correct binding complex conformations are of both higher sum of the interface degree values and lower characteristic path length than those incorrect ones. These features reflect that the correct bind-ing complex conformations have better geometric and/or residue type complementarity, and the correct binding modes are very important for preserving the characteristic path lengths of native protein complexes. In addition, two scoring terms are proposed based on the network parameters, in which the characteristics of the entire complex shape and residue type complementarity are taken into account. These network-based scoring terms have also been used in conjunction with other scoring terms, and the new multi-term scoring HPNCscore is devised in this work. It can improve the discrimination of the combined scoring function of RosettaDock more than 12%. This work might enhance our knowledge of the mechanisms of protein-protein interactions and recognition. | Shan Chang XinQi Gong Xiong Jiao ChunHua Li WeiZu Chen CunXin Wang | 2010 | Chinese Science Bulletin2010，55，9： | 3 |
| 5 | Prediction of PK-specific phosphorylation site based on information entropy显示文摘Phosphorylation is a crucial way to control the activity of proteins in many eukaryotic organisms in vivo. Experimental methods to determine phosphorylation sites in substrates are usually restricted by the in vitro condition of enzymes and very intensive in time and labor. Although some in silico methods and web servers have been introduced for automatic detection of phosphorylation sites, sophisticated methods are still in urgent demand to further improve prediction performances. Protein primary se-quences can help predict phosphorylation sites catalyzed by different protein kinase and most com-putational approaches use a short local peptide to make prediction. However, the useful information may be lost if only the conservative residues that are not close to the phosphorylation site are consid-ered in prediction, which would hamper the prediction results. A novel prediction method named IEPP (Information-Entropy based Phosphorylation Prediction) is presented in this paper for automatic de-tection of potential phosphorylation sites. In prediction, the sites around the phosphorylation sites are selected or excluded by their entropy values. The algorithm was compared with other methods such as GSP and PPSP on the ABL, MAPK and PKA PK families. The superior prediction accuracies were ob-tained in various measurements such as sensitivity (Sn) and specificity (Sp). Furthermore, compared with some online prediction web servers on the new discovered phosphorylation sites, IEPP also yielded the best performance. IEPP is another useful computational resource for identification of PK-specific phosphorylation sites and it also has the advantages of simpleness, efficiency and con-venience. | WANG MingHui, LI ChunHua, CHEN WeiZu & WANG CunXin College of Life Science and Bioengineering, Beijing University of Technology, Beijing 100022, China | 2008 | Science China(Life Sciences)2008，51，1： | 3 |
| 6 | MECHANISM OF BOUNDARY LUBRICATION UNDER POINT CONTACT显示文摘150SN 油和油酸的混合解决方案的酸值在解决方案,油酸的吸附的能力在 45 个钢球和磁盘基于被学习描绘油酸的卷内容。界限润滑测试在自我上被执行设计 ball-on-disk 机器。当添加剂被增加进润滑剂,基础油是纯 150SN 油,和油酸。磁盘有 0.8 亩 m and0.4 瓮的表面粗糙价值(R_a ) 。电的接触抵抗方法被用来决定润滑油地位。假设分子的电影是处于压缩状态和反对表面的单分子膜,这被分子的电影完全分开。一个界限润滑模型根据试验性的结果被建立并且假设。试验性、计算的结果证明钢表面上的极的分子的吸附是主要因素形成界限润滑电影。负担和滑动速度贡献很少到界限润滑的磨擦系数。为润滑剂的钢表面和添加剂的性质显著地在界限润滑的人物上影响。表面粗糙价值越小,界限润滑的磨擦系数是越多 smaller。 | WANG Weizu HUANG Ping | 2006 | Chinese Journal of Mechanical Engineering2006，19，4： | 2 |
| 7 | Sputtering Angular Distributions of Individual Elements for Low-Energy Ar Ion Irradiation of PtCu Alloy显示文摘The dynamic Monte Carlo program including a bombardment-induced Gibb-sian segregation process was used to^calculate angular distributions of individual elements from 3putlcrcd Cuq^PIq^alloy in the Ar ion energy range between 0.2 and 1.5ke V.Calculated results show that for each investigated incident energy,the angular distribution of the Pt element is more forward-pointed than that of the Cu one;the reason may be that different percentage of sputtered atoms(37-45% of Pt and 12-19% of Cu)come from beneath the topmost layer. | ZHENG Liping LI Risheng LI Mingyao SHEN Weizu LIN Junying ZOU Zhiyi | 1991 | Chinese Physics Letters1991，8，1： | 2 |
| 8 | A holistic molecular docking approach for predicting protein-protein complex structure显示文摘A holistic protein-protein molecular docking approach,HoDock,was established,composed of such steps as binding site prediction,initial complex structure sampling,refined complex structure sampling,structure clustering,scoring and final structure selection.This article explains the detailed steps and applications for CAPRI Target 39.The CAPRI result showed that three predicted binding site residues,A191HIS,B512ARG and B531ARG,were correct,and there were five submitted structures with a high fraction of correct receptor-ligand interface residues,indicating that this docking approach may improve prediction accuracy for protein-protein complex structures. | GONG XinQi,LIU Bin,CHANG Shan,LI ChunHua,CHEN WeiZu & WANG CunXin* College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124,China | 2010 | Science China(Life Sciences)2010，53，9： | 2 |
| 9 | A simulation method of combinding boundary element method with generalized Langevin dynamics显示文摘A new simulation approach to incorporate hydration force into generalized Langevin dynamics (GLD) is developed in this note. The hydration force determined by the boundary element method (BEM) is taken into account as the mean force terms of solvent including Coulombic interactions with the induced surface charge and the surface pressure of solvent. The exponential model is taken for the friction kernel. A simulation study has been performed on the cyclic undecapeptide cyclosporin A (CPA). The results obtained from the new method (GLDBEM) have been analyzed and compared with that obtained from the molecular dynamics (MD) simulation and the conventional stochastic dynamics (SD) simulation. We have found that the results obtained from GLDBEM show the obvious improvement over the SD simulation technique in the study of molecular structure and dynamic properties. | CHEN Weizu LU Benzhuo WANG Cunxin | 2000 | Chinese Science Bulletin2000，45，24： | 2 |
| 10 | Tensile creep due to restraining stresses in high-strength concrete at early ages显示文摘 | Zhang Tao Qin Weizu | 2005 | Cement and Concrete Research2005，，3： | 1 |
| 11 | Tensile creep due to restraining stresses in high-strcngtt concrete at early ages 显示文摘 | ZHANG Tao QIN Weizu | 2006 | Cement and Concrete Research2006，36，3： | 1 |
| 12 | A lock inside ' black box' hydrograph separation models : a study at the HydrohiU Catchment显示文摘 | Kendall C McDonnell J J Weizu Gu | 2001 | Hydrological Processes2001，15，： | 1 |
| 13 | Tensile creep due to restraining stresses in high-strength concrete at early ages显示文摘 | Tao Z Weizu Q | 2006 | Cement and Concrete Research2006，36，58： | 1 |
| 14 | Polarimetric calibration using agenetic algorithm 显示文摘 | XIONG Weizu | 2007 | IEEE Geoscience and Remote Sensing Letter2007，4，3： | 1 |
| 15 | Isotopic and hydrochemical data to restrict the origin of the groundwater in the Ba- dain Jaran Desert, Northern China显示文摘 | CHEN Jiansheng SUN Xiaoxu GU Weizu | 2012 | Geochemistry International2012，50，5： | 1 |
| 16 | Protective effects of ginsenoside Rg1 on chronic restraint stress induced learning and memory impairments in male mice显示文摘 | Yuchan Wang Hongwei Kan Yanyan Yin Wangyang Wu Wen Hu Mingming Wang Weiping Li Weizu Li | 2014 | Pharmacology, Biochemistry and Behavior2014，，： | 1 |
| 17 | Tensile creep due to restraining stresses in high-strength concrete at early ages显示文摘 | ZHANG Tao QIN Weizu | 2006 | Cement and Concrete Re- search2006，，36： | 1 |
| 18 | Uncertainities in flow modeling and forecasting for Niagara River显示文摘 | Randy D C Chaolin C Weizu Y | | 0，，11： | 1 |
| 19 | Protective effects of astragalosides on dexamethasone and Aβ25-35induced learning and memory impairments due to decrease amyloid precursor protein expression in 12-month male rats显示文摘 | Li Weizu Wu Wangyang Huang Dake | 2012 | Food and Chemical Toxicology2012，50，6： | 1 |
| 20 | Improvement of the relative entropy based protein folding method显示文摘The 'relative entropy' has been used as a minimization function to predict the tertiary structure of a protein backbone, and good results have been obtained. However, in our previous work, the ensemble average of the contact potential was estimated by an approximate calculation. In order to improve the theoretical integrity of the relative-entropy-based method, a new theoretical calculation method of the ensemble average of the contact potential was presented in this work, which is based on the thermodynamic perturbation theory. Tests of the improved algorithm were performed on twelve small proteins. The root mean square deviations of the predicted versus the native structures from Protein Data Bank range from 0.40 to 0.60 nm. Compared with the previous approximate values, the average prediction accuracy is improved by 0.04 nm. | QI LiSheng SU JiGuo CHEN WeiZu WANG CunXin | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，6： | 0 |
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