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| 1 | The RIP3-RIP1-NF-κB signaling axis is dispensable for necroptotic cells to elicit cross-priming of CD8^(+) T cells显示文摘Apoptosis and necroptosis are two types of programmed cell death with distinct morphological features.Necroptosis has been assumed to be more inflammatory than apoptosis,but a recent paper1 concluded that necroptotic cells cannot elicit cross-priming of CD8^(+)T cells and that NF-κB activation in necroptotic cells is required for maximal CD8^(+)T-cell cross-priming. | Junming Ren Xian Jia Yihao Zhao Wenke Shi Jiongcong Lu Yingying Zhang Jianfeng Wu Bo Liang Rui Wu Guo Fu Jiahuai Han | 2017 | Cellular & Molecular Immunology2017，14，7： | 1 |
| 2 | Integrated microfluidic single-cell immunoblotting chip enables high-throughput isolation,enrichment and direct protein analysis of circulating tumor cells显示文摘Effective capture and analysis of a single circulating tumor cell(CTC)is instrumental for early diagnosis and personalized therapy of tumors.However,due to their extremely low abundance and susceptibility to interference from other cells,high-throughput isolation,enrichment,and single-cell-level functional protein analysis of CTCs within one integrated system remains a major challenge.Herein,we present an integrated multifunctional microfluidic system for highly efficient and label-free CTC isolation,CTC enrichment,and single-cell immunoblotting(ieSCI).The ieSCI-chip is a multilayer microfluidic system that combines an inertia force-based cell sorter with a membrane filter for label-free CTC separation and enrichment and a thin layer of a photoactive polyacrylamide gel with microwell arrays at the bottom of the chamber for single-cell immunoblotting.The ieSCI-chip successfully identified a subgroup of apoptosis-negative(Bax-negative)cells,which traditional bulk analysis did not detect,from cisplatin-treated cells.Furthermore,we demonstrated the clinical application of the ieSCI-chip with blood samples from breast cancer patients for personalized CTC epithelial-to-mesenchymal transition(EMT)analysis.The expression level of a tumor cell marker(EpCAM)can be directly determined in isolated CTCs at the single-cell level,and the therapeutic response to anticancer drugs can be simultaneously monitored.Therefore,the ieSCI-chip provides a promising clinical translational tool for clinical drug response monitoring and personalized regimen development. | Aynur Abdulla Ting Zhang Shanhe Li Wenke Guo Antony RWarden Yufang Xin Nokuzola Maboyi Jiatao Lou Haiyang Xie Xianting Ding | 2022 | Microsystems & Nanoengineering2022，8，1： | 1 |
| 3 | Jointly Part-of-Speech Tagging and Semantic Role Labeling Using Auxiliary Deep Neural Network Model显示文摘Previous studies have shown that there is potential semantic dependency between part-of-speech and semantic roles.At the same time,the predicate-argument structure in a sentence is important information for semantic role labeling task.In this work,we introduce the auxiliary deep neural network model,which models semantic dependency between part-of-speech and semantic roles and incorporates the information of predicate-argument into semantic role labeling.Based on the framework of joint learning,part-of-speech tagging is used as an auxiliary task to improve the result of the semantic role labeling.In addition,we introduce the argument recognition layer in the training process of the main task-semantic role labeling,so the argument-related structural information selected by the predicate through the attention mechanism is used to assist the main task.Because the model makes full use of the semantic dependency between part-of-speech and semantic roles and the structural information of predicate-argument,our model achieved the F1 value of 89.0%on the WSJ test set of CoNLL2005,which is superior to existing state-of-the-art model about 0.8%. | Yatian Shen Yubo Mai Xiajiong Shen Wenke Ding Mengjiao Guo | 2020 | Computers, Materials & Continua2020，，10： | 0 |
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