|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Developmental stage-specific A-to-I editing pattern in the postnatal pineal gland of pigs(Sus scrofa)显示文摘Background: RNA editing is a widespread post-transcriptional modification mechanism in mammalian genomes.Although many editing sites have been identified in domestic pigs(Sus scrofa), little is known about the characteristics and dynamic regulation of RNA editing in the pineal gland(PG), a small neuroendocrine gland that synthesizes and secretes melatonin, which is primarily responsible to modulate sleep patterns.Results: This study analyzed the expression of adenosine-to-inosine(A-to-I) editing regulators and profiled the first dynamic A-to-I RNA editome during postnatal PG development. The results identified ADAR1 as the most abundantly expressed ADAR enzyme, which was down-regulated during postnatal PG development. Furthermore,47,284 high-confidence RNA editing sites were identified, the majority of which(93.6%) were of the canonical A-to-I editing type, followed by C-to-T editing. Analysis of its characteristics showed that the A-to-I editing sites mostly localized in SINE retrotransposons PRE-1/Pre0_SS. Moreover, a strong deficiency and preference for guanine nucleotides at positions of one base upstream or downstream were found, respectively. The overall editing level at the puberty stage was higher than at both infancy and adulthood stages. Additionally, genome-wide RNA editing was found to exhibit a dynamic stage-specific fashion(postnatally). Genes that underwent developmental changes in RNA editing were associated with catabolic processes as well as protein localization and transport functions,implying that RNA editing might be responsible for the molecular machineries of the postnatal developing PG.Remarkably, RNA editing in 3′-UTRs might regulate gene expression by influencing miRNA binding during PG development.Conclusions: This study profiles the first comprehensive developmental RNA editome in the pig PG, which contributes to the understanding of the importance of post-transcriptionally mediated regulation during mammalian postnatal PG development. Moreover, this study widely extends RNA editome resources in mammals. | Rong Zhou Wenye Yao Chundi Xie Leixia Zhang Yangli Pei Hua Li Zheng Feng Yalan Yang Kui Li | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 1 |
| 2 | Combination of artificial zeolite and microbial fertilizer to improve mining soils in an arid area of Inner Mongolia,China显示文摘Restoration of mining soils is important to the vegetation and environment.This study aimed to explore the variations in soil nutrient contents,microbial abundance,and biomass under different gradients of substrate amendments in mining soils to select effective measures.Soil samples were collected from the Bayan Obo mining region in Inner Mongolia Autonomous Region,China.Contents of soil organic matter(SOM),available nitrogen(AN),available phosphorus(AP),available potassium(AK),microbial biomass carbon/microbial biomass nitrogen(MBC/MBN)ratio,biomass,and bacteria,fungi,and actinomycetes abundance were assessed in Agropyron cristatum L.Gaertn.,Elymus dahuricus Turcz.,and Medicago sativa L.soils with artificial zeolite(AZ)and microbial fertilizer(MF)applied at T0(0 g/kg),T1(5 g/kg),T2(10 g/kg),and T3(20 g/kg).Redundancy analysis(RDA)and technique for order preference by similarity to ideal solution(TOPSIS)were used to identify the main factors controlling the variation of biomass.Results showed that chemical indices and microbial content of restored soils were far greater than those of control.The application of AZ significantly increases SOM,AN,and AP by 20.27%,23.61%,and 40.43%,respectively.AZ significantly increased bacteria,fungi,and actinomycetes abundance by 0.63,3.12,and 1.93 times of control,respectively.RDA indicated that AN,MBC/MBN ratio,and SOM were dominant predictors for biomass across samples with AZ application,explaining 87.6%of the biomass variance.SOM,MBC/MBN ratio,and AK were dominant predictors with MF application,explaining 82.9%of the biomass variance.TOPSIS indicated that T2 was the best dosage and the three plant species could all be used to repair mining soils.AZ and MF application at T2 concentration in the mining soils with M.sativa was found to be the most appropriate measure. | LI Wenye ZHANG Jianfeng SONG Shuangshuang LIANG Yao SUN Baoping WU Yi MAO Xiao LIN Yachao | 2023 | Journal of Arid Land2023，15，9： | 0 |
| 3 | Population genome of the newly discovered Jinchuan yak to understand its adaptive evolution in extreme environments and generation mechanism of the multirib trait显示文摘The adaptation and diversity of animals to the extreme environments of the Qinghai–Tibet Plateau(QTP)are typical materials to study adaptive evolution.The recently discovered Jinchuan yak population has many individuals with multiple ribs.However,little is known about this yak’s origin,evolution,and the genetic mechanisms that formed its unique multirib trait.Here,we report a valuable population genome resource of the Jinchuan yak by resequencing the whole genome of 150 individuals.Population genetic polymorphism and structure analysis reveal that Jinchuan yak can be differentiated as a unique and original yak population among the domestic yak.Combined with geological change,the Jinchuan yak’s evolutionary origin is speculated to be about 6290 years ago,which may be related to the unique geographical environment of the eastern edge of the QTP during this period.Compared with other domestic yaks,this new population has 280 positively selected genes.The genes related to skeletal function hold a considerable and remarkable proportion,suggesting that the specific skeletal characteristics have been enhanced in the adaptive evolution of Jinchuan yak in the extreme plateau environment.The genome-wide association study has revealed that TUBA8 and TUBA4A,the genes that regulate the cytoskeleton,are potential genes associated with the multirib trait.Our findings provide a basis to further understand the generation mechanism of the adaptive evolution of this new population in high-altitude extreme environments and the multivertebrate trait of domestic animals. | Daoliang LAN Wenhui JI Xianrong XIONG Qiqi LIANG Wenye YAO Tserang-Donko MIPAM Jincheng ZHONG Jian LI | 2021 | Integrative Zoology2021，16，5： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员