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您的检索式：作者名="XIAOWeiwei"
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| 1 | Design and application of 60mer oligonucleotide microarray in SARS coronavirus detection显示文摘The 60mer oligonucleotide microarray was designed and applied to detecting of SARS (severe acute respiratory syndrome) coronavirus. Thirty 60mer specific oligos were designed to cover the whole genome of the first submitted coronavirus strain, according to the sequence of TOR2 (GENEBANK Accession: AY274119). These primers were synthesized and printed into a microarray with 12×12 spots. RNAs were extracted from the throat swab and gargling fluid of SARS patients and reverse-transcripted into the double strand cDNAs. The cDNAs were prepared as restricted cDNA fragments by the restriction display (RD) technique and labeled by PCR with the Cy5-universal primer. The labeled samples were then applied to the oligo microarray for hybridization. The diagnostic capability of the microarray was evaluated after the washing and scanning steps. The scanning result showed that samples of SARS patients were hybridized with multiple SARS probes on the microarray, and there is no signal on the negative and blank controls. These results indicate that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide microarray, which can improve the positive ratio of the diagnosis. The oligo microarray can also be used for monitoring the behavior of the virus genes in different stages of the disease status. | SHIRong MAWenli WUQinghua ZHANGBao SONGYanbin GUOQiuye XIAOWeiwei WANGYan ZHENGWenling | 2003 | Chinese Science Bulletin2003，48，2： | 7 |
| 2 | A Rotation Scaling and Cropping Inavariant Second Generation Watermarking Scheme Based on Hough Transform显示文摘In this paper,a scheme which follows the seconde generation watermarking(2GW)paradigm is proposed.The goal of this proposed scheme is basically to increase the robustness against geometric attacks.The host image is decomposed with the wavelet packet.The bit stream of binary watermark is coded into several patterns with salient feature.The circular feature is used in this paper,because:(a)the computational complexity of the method grows rapidly with more complex shapes.(b) the circle is rotation -invariant and partially scale-invariant.(c)the circular feature can be detected by Hough transform effectively.These patterns are embedded into wavelet packet coefficients according to human perceptual charac teristics.A new HVS mask based on wavelet transform is proposed with consideration of local texture characteristics.The introduction of HVS caharacteristics boosts the performance of the whole scheme.Then,the watermarked image is generated by WP reconstruction.For the detection of watermark,the geometric distortion is calibrated for the contaminated watermarked image, then it is decomposed with the same WP used in embedding procedure.Hough transform is used to detect the circular features in the WP Coefficients.This scheme has the following characteristis:(a)robustness against the common geometric attacks(rotation,scaling,cropping,and etc)is improved significantly.(b)human perceptual characteristics is taken into consideration,so the tradeoff between invisibility and robustness is improved.Results of extensive experiments indicate that this proposed scheme is significantly effective in resisting various attacks such as rotation,scaling,JPEG compressing,adding noise,etc. | JIZhen ZHANGJihong XIAOWeiwei | 2003 | Chinese Journal of Electronics2003，12，1： | 2 |
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