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| 1 | Enhancing prime editing by Csy4-mediated processing of pegRNA显示文摘Dear Editor,The most advanced prime editor 3(PE3)system comprises the editor,a fusion protein of Cas9 H840A nickase and mutant reverse transcriptase(RTase)(hereafter termed NMRT),a prime editing guide RNA(pegRNA)and an alternative single-guide RNA(sgRNA).1 The pegRNA contains a primer binding site(PBS)and a reverse transcription(RT)template for introducing new genetic information1(Fig.1a;Supplementary information,Fig.S1a).We noted that the PBS,which is generally 10–16 nt at the 3′end of pegRNA,is complementary to part of the spacer at the 5′end of pegRNA,and their annealing is expected to cause pegRNA circularization,which can potentially hamper editing(Fig.1a;Supplementary information,Fig.S1b,c). | Yao Liu Guang Yang Shuhong Huang Xiangyang Li Xin Wang Guanglei Li Tian Chi Yulin Chen Xingxu Huang Xiaolong Wang | 2021 | Cell Research2021，31，10： | 5 |
| 2 | Complex field network-coded cooperation based on multi-user detection in wireless networks显示文摘Cooperative communication can achieve spatial diversity gains,and consequently combats signal fading due to multipath propagation in wireless networks powerfully.A novel complex field network-coded cooperation(CFNCC) scheme based on multi-user detection for the multiple unicast transmission is proposed.Theoretic analysis and simulation results demonstrate that,compared with the conventional cooperation(CC) scheme and network-coded cooperation(NCC) scheme,CFNCC would obtain higher network throughput and consumes less time slots.Moreover,a further investigation is made for the symbol error probability(SEP) performance of CFNCC scheme,and SEPs of CFNCC scheme are compared with those of NCC scheme in various scenarios for different signal to noise ratio(SNR) values. | Jing Wang Xiangyang Liu Kaikai Chi Xiangmo Zhao | 2013 | Journal of Systems Engineering and Electronics2013，24，2： | 2 |
| 3 | Decabromodiphenyl ether induces the chromosome association disorders of spermatocytes and deformation failures of spermatids in mice显示文摘The environmental presence of decabromodiphenyl ether(BDE-209),which is toxic to the male reproductive system,is widespread.The current study investigated its mechanism of toxicity in mice.The results showed,that BDE-209 induced DNA damage,decreased the expression of the promoter of meiosis spermatogenesis-and oogenesis-specific basic helix-loop-helix 1(Sohlh1),meiosis related-factors Lethal(3)malignant brain tumor like 2(L3MBTL2),PIWI-like protein 2(MILI),Cyclin-dependent kinase 2(CDK2),Cyclin A,synaptonemal complex protein 1(SYCP1)and synaptonemal complex protein 3(SYCP3),and caused spermatogenic cell apoptosis,resulting in a decrease in sperm quantity and quality.Furthermore,BDE-209 downregulated the levels of anaphase-promoting complex/cyclosome(APC/C),increased the expression of PIWI-like protein 1(MIWI)in the cytoplasm of elongating spermatids,and decreased the nuclear levels of RING finger protein 8(RNF8),ubiquitinated(ub)-H2A/ub-H2B,and Protamine 1(PRM1)/Protamine 2(PRM2),while increasing H2A/H2B nuclear levels in spermatids.The reproductive toxicity was persistent for 50 days following the withdrawal of BDE-209 exposure.The results suggested that BDE-209 inhibits the initiation of meiosis by decreasing the expression of Sohlh1.Furthermore,the reduced expression of L3MBTL2 inhibited the formation of chromosomal synaptonemal complexes by depressing the expression of meiosis regulators affecting the meiotic progression and also inhibited histone ubiquitination preventing the replacement of histones by protamines,by preventing RNF8 from entering nuclei,which affected the evolution of spermatids into mature sperm. | Jinglong Xue Xiangyang Li Yafei Chi Leqiang Gao Yue Zhang Yan Wang Moxuan Zhao Jialiu Wei Zhixiong Shi Xianqing Zhou | 2024 | Journal of Environmental Sciences2024，，4： | 0 |
| 4 | Complete L5 burst fracture treated by 270-degree decompression and reconstruction using titanium mesh cage via a single posterior vertebrectomy显示文摘 | Zeng Hanbing Wang Haibao Xu Huazi Chi Yonglong Mao Fangmin Wang Xiangyang | 2014 | Chinese Journal of Traumatology2014，17，5： | 0 |
| 5 | Broadly neutralizing antibodies against Omicron-included SARS-CoV-2 variants induced by vaccination显示文摘The SARS-CoV-2 Omicron variant shows substantial resistance to neutralization by infection-and vaccination-induced antibodies,highlighting the demands for research on the continuing discovery of broadly neutralizing antibodies(bnAbs).Here,we developed a panel of bnAbs against Omicron and other variants of concern(VOCs)elicited by vaccination of adenovirus-vectored COVID-19 vaccine(Ad5-nCoV). | Xiangyang Chi Yingying Guo Guanying Zhang Hancong Sun Jun Zhang Min Li Zhengshan Chen Jin Han Yuanyuan Zhang Xinghai Zhang Pengfei Fan Zhe Zhang Busen Wang Xiaodong Zai Xuelian Han Meng Hao Ting Fang Jinghan Xu Shipo Wu Yi Chen Yingying Fang Yunzhu Dong Bingjie Sun Jinlong Zhang Jianmin Li Guangyu Zhao Changming Yu Qiang Zhou Wei Chen | 2022 | Signal Transduction and Targeted Therapy2022，7，5： | 0 |
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