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| 1 | 全球尺度上氮添加影响植物生物量分配但不影响不同器官间的异速生长关系显示文摘生物量在不同器官间的分配是植物对环境变化响应的一个关键生态生理学过程。然而,在全球尺度上有关不同陆地生态系统植物生物量分配对氮沉降响应的认识还比较欠缺。本文通过整合分析333篇己发表文章的5474个观测值,基于“最优分配假说”和“异速分配假说”,评估了全球尺度上氮添加对植物生物量及其在不同器官间分配的影响。结果表明:(1)氮添加显著增加了整株植物或不同器官的生物量,降低了根冠比和根质量分数,但对叶质量分数和茎质量分数无显著影响;(2)氮添加对不同器官质量分数的影响受实验条件、植物功能性状、纬度、氮添加率等因子单独或交互作用的调控;(3)氮添加对生物量在不同器官间的异速分配率无显著影响,表明氮添加导致的根冠比和根质量分数减少是在异速分配模式下由整株植物生物量增加而致。虽然氮添加改变了地上和地下部分的质量分数,但不同器官生物量间异速分配模式的稳定性表明“异速分配假说”能更好地描述植物生物量分配对氮添加的响应规律。该研究结果将有助于深入认识氮沉降环境下植物生物量的分配规律,同时表明将生物量分配纳入有关氮沉降对陆地生态系统影响的预测模型中的重要性。 | Kai Yue Dario A.Fomara Wang Li Xiangyin Ni Yan Peng Shu Liao Siyi Tan Dingyi Wang Fuzhong Wu Yusheng Yang | 2021 | Journal of Plant Ecology2021，14，3： | 5 |
| 2 | Design of high performance multilayer microwave absorbers using fast Pareto genetic algorithm显示文摘The application of the Non-dominated Sorting Genetic Algorithm Ⅱ (NSGA-Ⅱ) in designing microwave absorbers is described in this paper. To obtain high performance coatings,we put forward three cost functions,which represent three objectives of strong-absorption,broad-bandwidth and thin structure,and study the tradeoffs between each other. Numerical calculations on available materials in 2―18 GHz are implemented to construct the Pareto front and Pareto-optimal surface for two and three objectives respectively. Results indicate that the NSGA-Ⅱ can work more efficiently and effectively than traditional Pareto genetic algorithms. Additionally,we present several particular designs from the above Pareto front (surface) for potential applications in different frequency bands. For example,a four-layer absorber with thickness of 2.8071 mm is obtained to provide average reflection coefficient of -11.95 dB and average reflection bandwidth of 0.5780 in 2―18 GHz,considering arbitrary incident angles (0°―89°) and both TE and TM polarizations. | JIANG LiYong LI XiangYin ZHANG Jie | 2009 | Science China(Technological Sciences)2009，52，9： | 2 |
| 3 | Design of broad omnidirectional total reflectors based on one-dimensional dielectric and magnetic photonic crystals显示文摘 | Qiang Haixia Jiang Liyong Li Xiangyin | 2010 | Optics & Laser Technology2010，42，1： | 1 |
| 4 | Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection显示文摘Antigen detection provides particularly valuable information for medical diagnoses;however,the current detection methods are less sensitive and accurate than nucleic acid analysis.The combination of CRISPR/Cas12a and aptamers provides a new detection paradigm,but sensitive sensing and stable amplification in antigen detection remain challenging.Here,we present a PCR-free multiple trigger dsDNA tandem-based signal amplification strategy and a de novo designed dual aptamer synergistic sensing strategy.Integration of these two strategies endowed the CRISPR/Cas12a and aptamer-based method with ultra-sensitive,fast,and stable antigen detection.In a demonstration of this method,the limit of detection was at the single virus level(0.17 fM,approximately two copies/μL)in SARS-CoV-2 antigen nucleocapsid protein analysis of saliva or serum samples.The entire procedure required only 20 min.Given our system’s simplicity and modular setup,we believe that it could be adapted reasonably easily for general applications in CRISPR/Cas12a-aptamer-based detection. | Xiangxiang Zhao Zhengduo Wang Bowen Yang Zilong Li Yaojun Tong Yuhai Bi Zhenghong Li Xuekui Xia Xiangyin Chen Lixin Zhang Weishan Wang Gao-Yi Tan | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 1 |
| 5 | Design of light trapping structures for light-absorption enhancement in thin film solar cells显示文摘 | Zhang Wei Zheng Gaige Li Xiangyin | 2013 | Optik-Intemational Journal for Light and Electron Optics2013，124，16： | 1 |
| 6 | Length of the ORF, position of the first AUG and the Kozak motif are important factors in potential dual-coding transcripts显示文摘一个单个哺乳动物的抄本通常编码一蛋白质,但是 GNAS (G 蛋白质伪 - 子单元) 的抄本包含二个读物框架并且生产二在结构上无关的蛋白质, XL 伪 s 和 ALEX。没有其它证实象 GNAS 一样双编码的抄本迄今为止被报导了,尽管许多如此的候选人基因被生物信息学分析预言了。在这研究,我们构造了一系列向量测试二个蛋白质产品怎么在 vitro 从一个单个抄本被翻译。ORF (开的读框架) 的长度,第一 8 月的位置和 Kozak 主题被发现是重要因素。这些因素,以及 55-bp NMD (调停胡说八道的 mRNA 腐烂) 规则,为候选人在生物信息学搜索被使用双编码的抄本。分别地, 1307, 750 和 474 个 two-ORF-containing 抄本的一个总数分别地, 170, 89 和 70 哪个被发现是潜在的双编码的抄本在人,老鼠和老鼠被发现。大多数抄本在种类之中显示出低保存。有趣地,双编码的抄本显著地从锌手指蛋白质家庭为抄本被充实,它通常是涉及抄写过程的规定的 DNA 有约束力的蛋白质。 | Heng Xu Ping Wang Yujie Fu Yufang Zheng Quan Tang Lizhen Si Jin You Zhenguo Zhang Yufei Zhu Li Zhou Zejun Wei Bin Lin Landian Hu Xiangyin Kong | 2010 | Cell Research2010，20，4： | 1 |
| 7 | Propagation properties of radial partially coherent flat-topped array beams in a turbulent atmosphere显示文摘 | Haiyan Wang Xiangyin Li | 2010 | Opt Commun2010，283，： | 1 |
| 8 | A novel image template mat- ching based on particle filtering optimization 显示文摘 | Li Hao Duan Haibin Zhang Xiangyin | 2010 | Pattern Recogni- tion Letters2010，31，13： | 1 |
| 9 | Teaching Reform of the Course of Introduction to Resource and Environment under 'STS' Quality Education Concept显示文摘With the purpose of cultivating students' 'STS' quality,multiple teaching methods were discussed,including the students-oriented teaching,group discussion,case teaching,interactive teaching,little teacher teaching,practical teaching and flexible assessment,etc.,thus the students learning initiative could be greatly stimulated,their comprehensive ability can be improved,and more excellent talents can be cultivated. | Xiangyin XI Shufeng WANG Limei LI Ying XU Meiyu LIU | 2013 | Asian Agricultural Research2013，5，5： | 1 |
| 10 | Structural and optical properties of ZnO thin films prepared by sol-gel method with different thickness 显示文摘 | Linhua Xu Xiangyin Li Yulin Chen Fei Xu | 2011 | Applied Surface Science2011，，9： | 1 |
| 11 | Nitrogen supply after removing the shoot apex increases the nicotine concentration and nitrogen contents in tobacco plants 显示文摘 | Xi Xiangyin Li Chunjian Zhang Fusuo | 2005 | Annals of Botany2005，96，： | 1 |
| 12 | Combinatorial analysis of transcription and metabolism reveals the regulatory network associated with antioxidant substances in waxy corn显示文摘Maize is an essential source of nutrition for humans and animals and is rich in various metabolites that determine its quality.Different maize varieties show significant differences in metabolite content.Two kinds of waxy maize parental materials,S181 and 49B,created by the Chongqing Academy of Agricultural Sciences,are widely grown in China.S181 shows higher starch and sugar contents than 49B.This study generated metabolic profiles to assess the differences between the two varieties.A total of 674 metabolites that were significantly differentially expressed between the two varieties were identified by gas chromatography and untargeted metabolomics technology.These metabolites were associated with 21 categories,including antioxidant metabolites.Moreover,6415 differentially expressed genes(DEGs)were identified by RNA-seq.Interestingly,these DEGs comprised starch and sugar synthesis pathway genes and 72 different transcription factor families.Among these,six families that were reported to play an essential role in plant antioxidant action accounted for 39.2%of the transcription factor families.Using the Kyoto Encyclopedia of Genes and Genomes(KEGG)classification,the DEGs were mainly involved in amino acid biosynthesis,glycolysis/glucose metabolism,and the synthetic and metabolic pathways of antioxidant active substances.Furthermore,the correlation analysis of transcriptome and metabolomics identified five key transcription factors(ZmbHLH172,ZmNAC44,ZmNAC-like18,ZmS1FA2,ZmERF172),one ubiquitin ligase gene(ZmE25A)and one sucrose synthase gene(ZmSS1).They likely contribute to the quality traits of waxy corn through involvement in the metabolic regulatory network of antioxidant substances.Thus,our results provide new insights into maize quality-related antioxidant metabolite networks and have potential applications for waxy corn breeding. | Jianhong Ke Rui Wang Bangqian Song Jinglun Du Xiaojiao Li Ningning Song Zhirong Cai Rongli Chen Honghua Yi Xiangyin Lu Chen Jiang Zhengguo Li Baowen Huang | 2022 | Food Quality and Safety2022，6，4： | 0 |
| 13 | In maize,co-expression of GAT and GR79-EPSPS provides high glyphosate resistance,along with low glyphosate residues显示文摘Herbicide tolerance has been the dominant trait introduced during the global commercialization of genetically modified(GM)crops.Herbicide-tolerant crops,especially glyphosate-resistant crops,offer great advantages for weed management;however,despite these benefits,glyphosate-resistant maize(Zea mays L.)has not yet been commercially deployed in China.To develop a new bio-breeding resource for glyphosate-resistant maize,we introduced a codon-optimized glyphosate N-acetyltransferase gene,gat,and the enolpyruvyl-shikimate-3-phosphate synthase gene,gr79-epsps,into the maize variety B104.We selected a genetically stable high glyphosate resistance(GR)transgenic event,designated GG2,from the transgenic maize population through screening with high doses of glyphosate.A molecular analysis demonstrated that single copy of gat and gr79-epsps were integrated into the maize genome,and these two genes were stably transcribed and translated.Field trials showed that the transgenic event GG2 could tolerate 9000 g acid equivalent(a.e.)glyphosate per ha with no effect on phenotype or yield.A gas chromatography-mass spectrometry(GC–MS)analysis revealed that,shortly after glyphosate application,the glyphosate(PMG)and aminomethylphosphonic acid(AMPA)residues in GG2 leaves decreased by more than 90%compared to their levels in HGK60 transgenic plants,which only harbored the epsps gene.Additionally,PMG and its metabolic residues(AMPA and N-acetyl-PMG)were not detected in the silage or seeds of GG2,even when far more than the recommended agricultural dose of glyphosate was applied.The co-expression of gat and gr79-epsps,therefore,confers GG2 with high GR and a low risk of herbicide residue accumulation,making this germplasm a valuable GR event in herbicide-tolerant maize breeding. | Shengyan Li Pengcheng Li Xiangyin Li Ning Wen Yinxiao Wang Wei Lu Min Lin Zhihong Lang | 2023 | aBIOTECH2023，4，4： | 0 |
| 14 | Computational prediction and validation of specific EmbR binding site on PknH显示文摘Tuberculosis drug resistance continues to threaten global health but the underline molecular mechanisms are not clear.Ethambutol(EMB),one of the well-known first-line drugs in tuberculosis treatment is,unfortunately,not free from drug resistance problems.Genomic studies have shown that some genetic mutations in Mycobacterium tuberculosis(Mtb)EmbR,and EmbC/A/B genes cause EMB resistance.EmbR-PknH pair controls embC/A/B operon,which encodes EmbC/A/B genes,and EMB interacts with EmbA/B proteins.However,the EmbR binding site on PknH was unknown.We conducted molecular simulation on the EmbR-peptides binding structures and discovered phosphorylated PknH 273-280(N′-HEALS^(P)DPD-C′)makesβstrand with the EmbR FHA domain,asβ-MoRF(MoRF;molecular recognition feature)does at its binding site.Hydrogen bond number analysis also supported the peptides’β-MoRF forming activity at the EmbR FHA domain.Also,we discovered that previously known phosphorylation residues might have their chronological order according to the phosphorylation status.The discovery validated that Mtb PknH 273-280(N′-HEALSDPD-C′)has reliable EmbR binding affinity.This approach is revolutionary in the computer-aided drug discovery field,because it is the first trial to discover the protein-protein interaction site,and find binding partner in nature from this site. | Insung Na Huanqin Dai Hantian Li Anvita Gupta David Kreda Powell Zhang Xiangyin Chen Lixin Zhang Gil Alterovitz | 2021 | Synthetic and Systems Biotechnology2021，6，4： | 0 |
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