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| 1 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 2 | Dietary lysozyme improves growth performance and intestinal barrier function of weaned piglets显示文摘Lysozyme(LZ)is a purely natural,nonpolluting and nonspecific immune factor,which has beneficial effects on the healthy development of animals.In this study,the influences of LZ on the growth performance and intestinal barrier of weaned piglets were studied.A total of 48 weaned piglets(Landrace×Yorkshire,22 d old)were randomly divided into a control group(basal diet)and a LZ group(0.1%LZ diet)for 19 d.The results showed that LZ could significantly improve the average daily gain(ADG,P<0.05)and average daily feed intake(ADFI,P<0.05).LZ also improved the intestinal morphology and significantly increased the expression of occludin in the jejunum(P<0.05).In addition,LZ down-regulated the expression of interleukin-1β(IL-1β,P<0.05)and tumor necrosis factor-a(TNF-a,P<0.05),and inhibited the expression of the genes in the nuclear factor-k-gene binding(NF-κB,P<0.05)signaling pathway.More importantly,the analysis of intestinal flora showed LZ increased the abundance of Firmicutes(P<0.05)and the ratio of Firmicutes to Bacteroidota(P=0.09)at the phylum level,and increased the abundance of Clostridium_sensu_stricto_1(P<0.05)and reduced the abundance of Olsenella and Prevotella(P<0.05)at the genus level.In short,this study proved that LZ could effectively improve the growth performance,relieve inflammation and improve the intestinal barrier function of weaned piglets.These findings provided an important theoretical basis for the application of LZ in pig production. | Yuying Wu Bei Cheng Longxiang Ji Xiangyun Lv Yingying Feng Liu’an Li Xin Wu | 2023 | Animal Nutrition2023，，3： | 0 |
| 3 | Generalized assembly of sandwich-like 0D/2D/0D heterostructures with highly exposed surfaces toward superior electrochemical performances显示文摘Heterostructures composed of two-dimensional(2D)nanosheets and zero-dimensional(0D)nanoparticles(NPs)have attracted increasing attention because of the synergy arising from the coupling interactions between the two mixed-dimensional components.Despite recent advances,it remains a challenge to fabricate 2D/0D heterostructures with clean and accessible surfaces,which is highly desirable for the diversity of catalytic,sensing,and energy storage applications.Herein,we report a generalized methodology that enables the facile assembly of sandwich-like 0D/2D/0D heterostructures with facilitated mass-transport channels and exposed surface active sites.A ligand-exchange strategy with HBF4 is employed to strip off the surface-coating ligands of colloidal NPs,rendering them positively charged and dispersible in polar solvents.This allows subsequent electrostatic assembly of NPs with oppositely charged 2D nanosheets to afford sandwich-like 0D/2D/0D heterostructures.The barely covered surfaces and the advantageous architectures of such sandwich-like 0D/2D/0D heterostructures induce the desired synergistic effect,making them particularly suitable for electrochemical energy storage and conversion.We demonstrate this by employing MXene/NiFe_(2)O_(4) and MXene/Fe3O4 heterostructures for high-performance electrocatalytic oxygen evolution and supercapacitors,respectively. | Shuqing Xue Guanhong Wu Mingzhong Li Zihan Liu Yuwei Deng Wenqian Han Xuanyu Lv Siyu Wan Xiangyun Xi Dong Yang Angang Dong | 2022 | Nano Research2022，15，1： | 0 |
| 4 | Cell metabolism pathways involved in the pathophysiological changes of diabetic peripheral neuropathy显示文摘Diabetic peripheral neuropathy is a common complication of diabetes mellitus.Elucidating the pathophysiological metabolic mechanism impels the generation of ideal therapies.However,existing limited treatments for diabetic peripheral neuropathy expose the urgent need for cell metabolism research.Given the lack of comprehensive understanding of energy metabolism changes and related signaling pathways in diabetic peripheral neuropathy,it is essential to explore energy changes and metabolic changes in diabetic peripheral neuropathy to develop suitable treatment methods.This review summarizes the pathophysiological mechanism of diabetic peripheral neuropathy from the perspective of cellular metabolism and the specific interventions for different metabolic pathways to develop effective treatment methods.Various metabolic mechanisms(e.g.,polyol,hexosamine,protein kinase C pathway)are associated with diabetic peripheral neuropathy,and researchers are looking for more effective treatments through these pathways. | Yaowei Lv Xiangyun Yao Xiao Li Yuanming Ouyang Cunyi Fan Yun Qian | 2024 | Neural Regeneration Research2024，19，3： | 0 |
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