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您的检索式：作者名="Xiaokang Ren"
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| 1 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 2 | On Water: Free Catalysis Approach for the Synthesis of Nitrones from Hydroxamic Acids and Dimethy/ Diethyl Acetylenedicarboxylate显示文摘Benzohydroxamic 酸面对 10 mol% NaOAc 与 dimethyl acetylenedicarboxylate 反应为 2 h 在房间温度在水里在 good-to-excellent 产量产生(E)-N-(1,4-dimethoxy-1,4-dioxobutan-2-ylidene)-1-phenylcarboxamide 氧化物,它供应一个简单、有效、环境地友好的方法综合大量 nitrones。这策略的好处不仅遵循绿 chemisty 的 requirment,而且拥有原子经济。 | Jian Li Yongqin He Xiaoyu Ren Xiaokang Shi Sizhuo Yang Xiai Gao Guosheng Huang | 2013 | Chinese Journal of Chemistry2013，31，8： | 1 |
| 3 | Assembly Induced Super-Large Red-Shifted Absorption:The Burgeoning Field of Organic Near-Infrared Materials显示文摘Supramolecular assembly of organic dye compounds with J-aggregation leads to a red-shifted absorption spectrum that greatly facilitates the construction of near-infrared(NIR)materials.A considerable improvement of the material functions requires that the absorption red-shift be larger than 100 nm,but such a super-large red-shift is challenging,and the rules leading to the super-large red-shifted absorption is still not explicit.In this review,we focused on those J-aggregated organic dye materials with super-large red-shifted absorption.The nature of the super-large red-shift is originated fromthe intermolecular charge transfer between neighboring chromophores.The super-large red-shift can be obtained by tuning either the molecular structure or kinetic assembly process in a delicate manner.Materials with super-large red-shifted absorption have been successfully applied to biological imaging,phototherapy,electronic devices,and solar cells,and show great potential in many other fields.The elaboration of assembly induced super-large red-shifted absorption is promising for design of supramolecular NIR materials with tuned structures,enhanced functionalities,and a wide array of applications. | Luyang Zhao Xiaokang Ren Xuehai Yan | 2021 | CCS Chemistry2021，3，5： | 0 |
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