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| 1 | Acupuncture at the Taixi(KI3) acupoint activates cerebral neurons in elderly patients with mild cognitive impairment显示文摘Our previous findings have demonstrated that acupuncture at the Taixi(KI3) acupoint in healthy youths can activate neurons in cognitive-related cerebral cortex. Here, we investigated whether acupuncture at this acupoint in elderly patients with mild cognitive impairment can also activate neurons in these regions. Resting state and task-related functional magnetic resonance imaging showed that the pinprick senstation of acupuncture at the Taixi acupoint differed significantly between elderly patients with mild cognitive impairment and healthy elderly controls. Results showed that 20 brain regions were activated in both groups of participants, including the bilateral anterior cingulate gyrus(Brodmann areas [BA] 32, 24), left medial frontal cortex(BA 9, 10, 11), left cuneus(BA 19), left middle frontal gyrus(BA 11), left lingual gyrus(BA 18), right medial frontal gyrus(BA 11), bilateral inferior frontal gyrus(BA 47), left superior frontal gyrus(BA11), right cuneus(BA 19, 18), right superior temporal gyrus(BA 38), left subcallosal gyrus(BA 47), bilateral precuneus(BA 19), right medial frontal gyrus(BA 10), right superior frontal(BA 11), left cingulate gyrus(BA 32), left precentral gyrus(BA 6), and right fusiform gyrus(BA 19). These results suggest that acupuncture at the Taixi acupoint in elderly patients with mild cognitive impairment can also activate some brain regions. | Shangjie Chen Maosheng Xu Hong Li Jiuping Liang Liang Yin Xia Liu Xinyan Jia Fen Zhu Dan Wang Xuemin Shi Lihua Zhao | 2014 | Neural Regeneration Research2014，9，11： | 18 |
| 2 | Future 5G-Oriented System for Urban Rail Transit:Opportunities and Challenges显示文摘As a development direction of urban rail transit system,the train autonomous circumambulate system(TACS)can operate in a safer,more efficient,and more economical mode.However,most urban rail transit systems transmit signals through industrial,scientific,and medical(ISM)frequency bands or narrow frequency bands,which cannot meet the requirements of TACS.As a promising solution,the 5th generation(5G)mobile communication provides more services for the future urban rail transit systems,and covers the shortages of exiting communication technologies in terms of capacity and reliability.In this paper,we first briefly review the research status of current train control system and introduce its limitations.Next,we propose a novel network architecture,and present new technologies and requirements of the proposed architecture for TACS.Some potential challenges are then discussed to give insights for further research of TACS. | Junhui Zhao Jin Liu Lihua Yang Bo Ai Shanjin Ni | 2021 | China Communications2021，18，2： | 18 |
| 3 | Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting显示文摘Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system to target the JHregion of the pig Ig M heavy chain gene which is crucial for B cell development and differentiation. Transfection of Ig M-targeting Cas9 plasmid in primary porcine fetal fibroblasts(PFFs) enabled inducing gene knock out(KO) in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination(HR). With the aid of somatic cell nuclear transfer(SCNT) technology, three piglets with the biallelic Ig M heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the Ig M heavy chain gene effectively knocked out the function of the Ig M and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs. | Fengjiao Chen Ying Wang Yilin Yuan Wei Zhang Zijian Ren Yong Jin Xiaorui Liu Qiang Xiong Qin Chen Manling Zhang Xiaokang Li Lihua Zhao Ze Li Zhaoqiang Wu Yanfei Zhang Feifei Hu Juan Huang Rongfeng Li Yifan Dai | 2015 | Journal of Genetics and Genomics2015，42，8： | 15 |
| 4 | H2A.Z Represses Gene Expression by Modulating Promoter Nucleosome Structure and Enhancer Histone Modifications in Arabidopsis显示文摘 | Xiaozhuan Dai Youhuang Bai Lihua Zhao Xianying DOU Yanhui Liu Lulu Wang Yi Li Weimin Li Yanan Hui Xinyu Huang Zonghua Wang Yuan Qin | 2017 | Molecular Plant2017，10，10： | 14 |
| 5 | The impact of land use change on soil organic carbon and labile organic carbon stocks in the Longzhong region of Loess Plateau显示文摘Land use change (LUC) is widely recognized as one of the most important driving forces of global carbon cycles. The soil organic carbon (SOC) and labile organic carbon (LOC) stores were investigated at arable land (AL), artificial grassland (AG), artificial woodland (AW), abandoned arable land (AAL) and desert steppe (DS) in the Longzhong region of the Loess Plateau in Northwest China. The results showed that conversions from DS to AL, AL to AG and AL to AAL led to an increase in SOC content, while the conversion from DS to AW led to a decline. The differences in SOC content were significant between DS and AW at the 20-40 cm depth and between AL and AG at the 0-10 cm depth. The SOC stock in DS at the 0-100 cm depth was 39.4 t/hm 2 , increased by 28.48% after cultivation and decreased by 19.12% after conversion to AW. The SOC stocks increased by 2.11% from AL to AG and 5.10% from AL to AAL. The LOC stocks changed by a larger magnitude than the SOC stocks, which suggests that it is a more sensitive index of carbon dynamics under a short-term LUC. The LOC stocks increased at 0-20 cm and 0-100 cm depths from DS to AW, which is opposite to that observed for SOC. The proportion of LOC to SOC ranged from 0.14 to 0.20 at the 0-20 cm depth for all the five land use types, indicating low SOC dynamics. The allocation proportion of LOC increased for four types of LUC conversion, and the change in magnitude was largest for DS to AW (40.91%). The afforestation, abandonment and forage planting on arable land led to sequestration of SOC; the carbon was lost initially after afforestation. However, the carbon sink effect after abandonment may not be sustainable in the study area. | LiHua ZHANG ZhongKui XIE RuiFeng ZHAO YaJun WANG | 2012 | Journal of Arid Land2012，4，3： | 13 |
| 6 | Joint land-sea seismic survey and research on the deep structures of the Bohai Sea areas显示文摘This paper presents the survey and research work of two land-sea profiles in the Bohai Sea,China,carried out in 2010–2011,including the seismic sources on land and in the sea,the ocean bottom seismographs(OBS)and their recovery,the coupling of OBS and the environment noise in sea area,the data quality of OBSs,and the result of data analysis.We focused on the investigation of crustal structures revealed by the two NE\EW-trending joint land-sea profiles.In combination with the Pn-velocity distribution and gravitymagnetic inversion results in the North China Craton,we propose that the undulation of the Moho interface in the Bohai and surrounding areas is not strong,and the lithospheric thinning is mainly caused by the thinning of its mantle part.The research result indicates that obvious lateral variations of Moho depth and seismic velocity appear nearby all the large-scale faults in Bohai Sea,and there is evidence of underplating and reforming of the lower crust by mantle material in the Bohai area.However,geophysical evidence does not appear to support the'mantle plume'or'delamination'model for the North China Craton destruction.The crustal structure of the Bohai Sea revealed'a relatively normal crust and obviously thinned mantle lid',local velocity anomalies and instability phenomena in the crust.These features may represent a combined effect of North China-Yangtze collision at an early stage and the remote action of Pacific plate subduction at a late stage. | HAO Tianyao YOU Qingyu LIU Lihua LV Chuanchuan XU Ya LI Zhiwei ZHAO Chunlei ZHENG Yanpeng LIU Chenguang HAN Guozhong | 2013 | Acta Oceanologica Sinica2013，32，12： | 11 |
| 7 | Extraction of copper from sewage sludge using biodegradable chelant EDDS显示文摘[S,S]-Ethylenediaminedisuccinic acid(EDDS),a biodegradable chelant,was used to separate the heavy metals from the sewage sludge based on chemical extraction technology.Under various conditions,the extraction experiments were carried out for the sewage sludge from Shanghai Taopu Municipal Wastewater Plant,China.The influences ofpH and the concentration of EDDS on the extraction efficiency for copper(Cu)were discussed.The results showed that EDDS had higher extraction efficiency for Cu from the sewage slud... | ZHANG, Lihua ZHU, Zhiliang ZHANG, Ronghua ZHENG, Chengsong ZHANG, Hua QIU, Yanling ZHAO, Jianfu | 2008 | Journal of Environmental Sciences2008，20，8： | 11 |
| 8 | Reactive oxygen species-based nanomaterials for the treatment of myocardial ischemia reperfusion injuries显示文摘Interventional coronary reperfusion strategies are widely adopted to treat acute myocardial infarction,but morbidity and mortality of acute myocardial infarction are still high.Reperfusion injuries are inevitable due to the generation of reactive oxygen species(ROS)and apoptosis of cardiac muscle cells.However,many antioxidant and anti-inflammatory drugs are largely limited by pharmacokinetics and route of administration,such as short half-life,low stability,low bioavailability,and side effects for treatment myocardial ischemia reperfusion injury.Therefore,it is necessary to develop effective drugs and technologies to address this issue.Fortunately,nanotherapies have demonstrated great opportunities for treating myocardial ischemia reperfusion injury.Compared with traditional drugs,nanodrugs can effectively increase the therapeutic effect and reduces side effects by improving pharmacokinetic and pharmacodynamic properties due to nanodrugs’size,shape,and material characteristics.In this review,the biology of ROS and molecular mechanisms of myocardial ischemia reperfusion injury are discussed.Furthermore,we summarized the applications of ROS-based nanoparticles,highlighting the latest achievements of nanotechnology researches for the treatment of myocardial ischemia reperfusion injury. | Tianjiao Zhao Wei Wu Lihua Sui Qiong Huang Yayun Nan Jianhua Liu Kelong Ai | 2022 | Bioactive Materials2022，7，1： | 11 |
| 9 | Biallelic mutations in CDC20 cause female infertility characterized by abnormalities in oocyte maturation and early embryonic development显示文摘Dear Editor,Previously,the Mendelian phe no types in huma n oocyte maturation arrest,fertilization failure and early embryonic arrest,are largely underestimated.In recent years,'missing'Men delian phe no types and genes in these processes are beginning to be uncovered by us and others(Huang et al.,2014;Alazami et al..2015;Feng et al.,2016;Xu et al.,2016;Chen et al.,2017;Sang et al.,2019).However,the genetic basis for majority of patients resulting from abnormalities in these phe no types remains to be elucidated. | Lin Zhao Songguo Xue Zhongyuan Yao Juanzi Shi Biaobang Chen Ling Wu Lihua Sun Yao Xu Zheng Yan Bin Li Xiaoyan Mao Jing Fu Zhihua Zhang Jian Mu Wenjing Wang Jing Du Shuai Liu Jie Dong Weijie Wang Qiaoli Li Lin He Li Jin Xiaozhen Liang Yanping Kuang Xiaoxi Sun Lei Wang Qing Sang | 2020 | Protein & Cell2020，11，12： | 10 |
| 10 | Evolution of Surface Sensible Heat over the Tibetan Plateau Under the Recent Global Warming Hiatus显示文摘Based on regular surface meteorological observations and NCEP/DOE reanalysis data, this study investigates the evolution of surface sensible heat(SH) over the central and eastern Tibetan Plateau(CE-TP) under the recent global warming hiatus. The results reveal that the SH over the CE-TP presents a recovery since the slowdown of the global warming. The restored surface wind speed together with increased difference in ground-air temperature contribute to the recovery in SH.During the global warming hiatus, the persistent weakening wind speed is alleviated due to the variation of the meridional temperature gradient. Meanwhile, the ground surface temperature and the difference in ground-air temperature show a significant increasing trend in that period caused by the increased total cloud amount, especially at night. At nighttime, the increased total cloud cover reduces the surface effective radiation via a strengthening of atmospheric counter radiation and subsequently brings about a clear upward trend in ground surface temperature and the difference in ground-air temperature.Cloud–radiation feedback plays a significant role in the evolution of the surface temperature and even SH during the global warming hiatus. Consequently, besides the surface wind speed, the difference in ground-air temperature becomes another significant factor for the variation in SH since the slowdown of global warming, particularly at night. | Lihua ZHU Gang HUANG Guangzhou FAN Xia QU Guijie ZHAO Wei HUA | 2017 | Advances in Atmospheric Sciences2017，34，10： | 8 |
| 11 | A novel two-layered optical satellite network of LEO/MEO with zero phase factor显示文摘Optical satellite communication has advantages over the RF satellite communication in capacity, speed, secrecy and anti-interference.Space information superhighway composed of GEO/MEO/LEO satellites which are linked by optical inter-satellite links(OISL) would play important roles in communication, navigation, martial recon, remote sensing and deep space exploration.A novel LEO/MEO two-layered optical satellite network with global coverage is designed with the street-of-coverage technique.The layer of LEO satellites is the walker-delta constellation with zero phase factor and has the quasi-stable logical structure of Mesh.Equatorial and polar orbit satellites constitute the layer of MEO satellite, which can cover LEO layer effectively.Rising satellites of the system can provide 99.9% of coverage for the whole globe and 100% for China averagely.The network can provide a minimum elevation angle at 21 ? with single-satellite visibility for China, and at 16 ? with the dual-satellite visibility.Simulation results of the inter-satellite links show LEO constellation of zero phase factor outperforms non-zero phase factor constellations in azimuth angle, elevation angle and distance, which is very fit for the OISL.Simulation results of the communication performance indicate a little difference between short wave band and long wave band at low communication speed(several Gbit/s);however, the wave band at 1550 nm is a better choice at high communication speed(100 Gbit/s). | LI YongJun , WU JiLi, ZHAO ShangHong, MENG Wen, MA LiHua, SHI Lei, CHU XingChun, HOU Rui & LI Tian Telecommunication Engineering Institute, Air Force Engineering University, Xi’an 710077, China | 2010 | Science China(Information Sciences)2010，53，6： | 8 |
| 12 | Estimation of coal particle size distribution by image segmentation显示文摘Several industrial coal processes are largely determined by the distribution of particle sizes in their feed.Currently these parameters are measured by manual sampling,which is time consuming and cannot provide real time feedback for automatic control purposes.In this paper,an approach using image segmentation on images of overlapped coal particles is described.The estimation of the particle size distribution by number is also described.The particle overlap problem was solved using image enhancement algorithms that converted those image parts representing material in lower layers to black.Exponential high-pass filter(EHPF) algorithms were used to remove the texture from particles on the surface.Finally,the edges of the surface particles were identified by morphological edge detection.These algorithms are described in detail as is the method of extracting the coal particle size.Tests indicate that using more coal images gives a higher accuracy estimate.The positive absolute error of 50 random tests was consistently less than 2.5% and the errors were reduced as the size of the fraction increased. | Zhang Zelin Yang Jianguo Ding Lihua Zhao Yuemin | 2012 | International Journal of Mining Science and Technology2012，22，5： | 7 |
| 13 | Theoretical analysis of a new segmented anchoring style in weakly cemented soft surrounding rock显示文摘According to the tensile failure of rock bolt in weakly cemented soft rock, this paper presents a new segmented anchoring style in order to weaken the cumulative effect of anchoring force associated with the large deformation. Firstly, a segmented mechanical model was established in which free and anchoring section of rock bolt were respectively arranged in different deformation zones. Then, stress and displacement in elastic non-anchoring zone, elastic anchoring zone, elastic sticking zone, softening sticking zone and broken zone were derived respectively based on neural theory and tri-linear strain softening constitutive model of soft rock. Results show that the anchoring effect can be characterized by a supporting parameter b. With its increase, the peak value of tangential stress gradually moves to the roadway wall, and the radial stress significantly increases, which means the decrease of equivalent plastic zone and improvement of confining effect provided by anchorage body. When b increases to 0.72, the equivalent plastic zone disappears, and stresses tend to be the elastic solutions. In addition, the anchoring effect on the displacement of surrounding rock can be quantified by a normalization factor δ. | Zhao Zenghui Wang Weiming Wang Lihua | 2016 | International Journal of Mining Science and Technology2016，26，3： | 7 |
| 14 | Advertisement Call Variability in the Black-spined Toad Bufo melanostictus (Anura: Bufonidae) during the Breeding Season in Lishui, Zhejiang, China显示文摘Advertisement calls play an important role in influencing female mate choice and are regarded as one of the key characters responsible for reproductive isolation and speciation events in frogs and toads. The black-spined toad, Bufo melanostictus, is one of the most common amphibians in Southeast Asia and is frequently observed in human habitations throughout its distributional range. The advertisement calls of the toad have not been well described. Here, we report the advertisement calls of this species from Lishui, Zhejiang, China during its breeding season. The advertisement calls of B. melanostictus are composed of single notes with a single harmonic frequency emitted sporadically. The call parameters recorded in this study were highly variable across recording times and with different body sizes. Dominant frequency was negatively correlated with body size. Comparing the calls of the B. melanostictus population from Lishui with the populations from five other localities(Bangkok, Thailand; Coorg, India; Burma, Myanmar; Bali, Indonesia; Halimun-Salak National Park, Indonesia), we found that call structure was similar among five different populations with no harmonics, while the Halimun-Salak National Park population contained two clear harmonics. Dominant frequency was similar between Bali(1.56 kHz) and Coorg(1.6 kHz) populations, while it was highly variable among other populations. Besides call duration, call interval and pulse/call rate may also be significantly different among the comparing populations. Pooling the available data, we observed some similarities and differences in call parameters among the six populations. | Li WEI Lihua ZHAO Xiaohao MA Xiaoli FAN Xiaomei MA Zhihua LIN | 2012 | Asian Herpetological Research2012，3，2： | 6 |
| 15 | Assessment of wetland fragmentation in the middle reaches of the Heihe River by the type change tracker model显示文摘The quantitative research of wetland landscape fragmentation in the middle reaches of the Heihe River is important for the wetland and oasis sustainable development in the Hexi Corridor. Based on the data of remote sensing and GIS, we constructed the type change tracker model with sliding window technique and spatially morphological rule. The suitable scale and optimum scale of the fragmentation model of wetland landscape in the middle reaches of the Heihe River were determined by the area frequency statistics method, Chi-square distribution normalized scale variance, fractal dimension and diversity index. By integrating type change tracker model and the optimum scale with GIS spatial analysis, the spatial distribution characteristics of wetland landscape fragmentation in different periods and the related spatial-temporal change process were clarified. The results showed that(1) the type change tracker model, which analyzes the spatial pattern of wetland fragmentation on the pixel level, is better than the traditional wetland fragmentation analysis on the landscape and patch levels;(2) The suitable scale for the wetland fragmentation ranged from 150 m×150 m to 450 m×450 m and the optimum scale was 250 m×250 m in the middle reaches of the Heihe River; and(3) In the past 35 years, the total wetland area decreased by 23.2% and the fragmentation of wetland markedly increased in the middle reaches of the Heihe River. The areas of core wetlands reduced by 12.8% and the areas of perforated, edge and patch wetlands increased by 0.8%, 3.1% and 8.9%, respectively. The process of wetland fragmentation in the research region showed the order of core wetland, perforated or edge wetland, patch wetland or non-wetland. The results of this study would provide a reference for the protection, utilization and restoration of limited wetland resources and for the sustainable development of the regional eco-environment in the Heihe River Basin. | RuiFeng ZHAO ZuoLun XIE LiHua ZHANG Wen ZHU Jie LI Dan LIANG | 2015 | Journal of Arid Land2015，7，2： | 6 |
| 16 | Character of the Si and Al Phases in Coal Gangue and Its Ash显示文摘Analysis of the Si and Al phases in coal gangue fuel and its ash is important for use of coal gangue ashes. A comprehensive study by theoretical and experimental analyses with differential thermal analysis, X-ray diffraction and Infrared Spectroscopy has been made in the present article to explore the diagram of the Si and Al phases in coal gangue fuel and its ashes. It is found that kaolinite and quartz are the main phases in coal gangue fuel. The ratio of moles Al2O3 to SiO2 (i.e., Al2O3 (mole)/SiO2 (mole)) is usually no more than 0.5 in most coal gangue fuel and its ashes. The kaolinit at about 984°C releases a large quantity of SiO2, which makes calcine coal gangue more active than coal gangue itself. The relationship between the ratio Al2O3 (mole)/SiO2(mole) and the components of coal gangue ash is analyzed, resulting in a formula to calculate the quantity of each phase. Applying the formula to the testing samples from an electric plant in north China supports the above conclusions. | WANG Lihua ZHAO Pengda LI Geng | 2009 | Acta Geologica Sinica(English Edition)2009，83，6： | 6 |
| 17 | Integrated genomic analysis identifies deregulated JAK/STAT-MYC-biosynthesis axis in aggressive NK-cell leukemia显示文摘好攻击的 NK 房间白血病(ANKL ) 是在从亚洲的人之中是更流行的 NK 房间瘤的一种稀罕形式并且中央并且南美洲。病人们通常在天以内死到月,甚至在收到迅速的治疗学的管理以后。这里,我们由集成整个染色体执行了 ANKL 的第一全面研究, transcriptome 并且定序指向,象功能的试金一样的 cytokine 数组。在 JAK-STAT 小径的变化在 48% 被识别(14/29 ) ANKL 病人,当细胞外的 STAT3 激发器 IL10 被 56 褶层的一般水准提高时(P < 0.0001 ) 在检查的所有病人的血浆。另外的经常变异的基因包括的 TP53 (34%) , TET2 (28%) , CREBBP (21%) 和 MLL2 (21%) 。耐心的 NK 白血病房间在多重新陈代谢的小径显示出 STAT3 phosphorylation, MYC 表示和 transcriptional 活动的突出的激活。机能上地, STAT3 激活和 MYC 表示为 ANKL 房间的增长和幸存是批评的。发信号的 STAT 调整了发信号的 MYC 抄写节目,和两 STAT, MYC 抄写被要求维持核苷酸合成和 glycolysis 的激活。一起, JAK-STAT 小径在 ANKL 为 genomic 改变和 IL10 刺激代表一个主要目标。这最新发现的 JAK/STAT-MYC-biosynthesis 轴可以在对待白血病的这种子类型为新奇治疗学的策略的发展提供机会。 | Liang Huang Dan Liu Na Wang Shaoping Ling Yuting Tang Jun Wu Lingtong Hao Hui Luo Xuelian Hu Lingshuang Sheng Lijun Zhu Di Wang Yi Luo Zhen Shang Min Xiao Xia Mao Kuangguo Zhou Lihua Cao Lili Dong Xinchang Zheng Pinpin Sui Jianlin He Shanlan Mo Jin Yan Qilin Ao Lugui Qiu Hongsheng Zhou Qifa Liu Hongyu Zhang Jianyong Li Jie Jin Li Fu Weili Zhao Jieping Chen Xin Du Guoliang Qing Hudan Liu Xin Liu Gang Huang Ding Ma Jianfeng Zhou Qian-fei Wang | 2018 | Cell Research2018，28，2： | 5 |
| 18 | Large numbersLarge numbers of new bacterial taxa found by Yunnan Institute of Microbiology显示文摘Extreme environments, primeval forest, sea sediments and plants in China yielded members of a new suborder, Jiangellineae, a new family Sinobacteraceae, and 23 new genera of actinomycetes and other bacteria. Three suggestions were made for further discoveries of new actinomycetes. First, natural habitats such as old growth forests, and extreme environments, should be given sampling priority; second, culturing procedures need to be constantly improved to mimic natural habitats; third, less than 98.5% similarity of 16S rRNA sequence to that of a known species could be used as an indicator of a new species. | JIANG Yi CAO YanRu ZHAO LiXing TANG ShuKun WANG Yun LI WenJun XU Ping LOU Kai MAO PeiHong XU LiHua | 2011 | Chinese Science Bulletin2011，56，8： | 5 |
| 19 | Structure insights into mechanisms of ATP hydrolysis and the activation of human heat-shock protein 90显示文摘分子的女伴热吃惊蛋白质 90 的激活(Hsp90 ) 依赖于 ATP 绑定和水解作用,它发生在蛋白质的 N 终端域。这里,我们与 ATP 和 ATP 类似物在土著人并且在建筑群决定了人的 Hsp90 的 N 终端领域的三水晶结构,提供由 Hsp90 的 ATP 水解作用的催化机制的一个清楚的看法。另外, ATP 的绑定导致 N 终端领域是能被用来部分解释 Hsp90 的孤立的 N 终端领域为什么举办很弱的 ATP hydrolytic 活动的一个中间的状态。 | Jian Li Lihua Sun Chunyan Xu Feng Yu Huan Zhou Yanlong Zhao Jian Zhang Jianhua Cai Cheney Mao Lin Tang Yechun Xu Jianhua He | 2012 | Acta Biochimica et Biophysica Sinica2012，44，4： | 5 |
| 20 | Effect of chitooligosaccharides on cyclin D1,bcl-xl and bcl-2 mRNA expression in A549 cells using quantitative PCR显示文摘Chitooligosaccharide (COS) is derived from the chemical and enzymatic hydrolysis of chitosan and has been reported to have potent antitumor activity.Our study investigated the effects of five chitooligomers ranging from the dimer form to the hexamer form (chitobiose,chitotriose,chitotetraose,chitopentaose,chitohexaose) on the expression of cyclin D1,bcl-2 and bcl-xl mRNA in A549 cells using reverse transcription quantitative real-time PCR.We demonstrated that,of the five chitooligomers used,chitohexaose (COS6) had the most potent inhibitory effect on A549 cell proliferation.COS6 also significantly down regulated cyclin D1 and bcl-xl mRNA expression levels.Our data suggested that COS6 exerts its antitumor activity by two different mechanisms:(1) COS6-mediated inhibition of Cyclin D1 levels leads to suppression of tumor cell proliferation;and (2) COS6-mediated down-regulation of the pro-survival protein,Bcl-xl,promotes the apoptosis of tumor cells. | LI Xian WANG Ju CHEN XiaoJia TIAN JinHuan LI LiHua ZHAO MingYan JIAO YanPeng ZHOU ChangRen | 2011 | Chinese Science Bulletin2011，56，15： | 5 |
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