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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | Induced Th2 dominant immune response in APPswe, PSEN1dE9 transgenic mice after nasal immunization with an adenoviral vector encoding 10 tandem repeats of beta-amyloid 3-10显示文摘Immunotherapy for Alzheimer's disease (AD) is effective in improving cognitive function in transgenic mouse models of AD. Because the AN1792 [beta-amyloid (Aβ) 1-42] vaccine was halted because of T cell mediated meningoencephalitis, many scientists are searching for a novel vaccine to avoid the T cell mediated immune response caused by the Aβ1-42. Importantly, the time when the immunization is begun can influence the immune effect. In this study, an adenovirus vaccine was constructed containing 10 × Aβ3-10 repeats and gene adjuvant CpG DNA. Transgenic AD mice were immunized intranasally for 3 months. After 10 × Aβ3-10 vaccine immunization, high titers of anti-Aβ42 IgG1 predominant antibodies were induced. In spatial learning ability and probe tests, the 10 × Aβ3-10 immunized mice showed significantly improved memories compared to control mice. The 10 × Aβ3-10 vaccine resulted in a robust Th2 dominant humoral immune response and reduced learning deficits in AD mice. In addition, the 10 × Aβ3-10 vaccine might be more efficient if administered before Aβ aggregation at an early stage in the AD mouse brain. Thus, the adenovirus vector encoding 10 × Aβ3-10 is a promising vaccine for AD. | Rong Guo Kui Huang Tongzi Jiang Jian Li Yu Li Xiaona Xing Yunpeng Cao | 2011 | Neural Regeneration Research2011，6，26： | 2 |
| 3 | Comparison of lateral episiotomy,traditional midwifery without episiotomy,and hands-off techniques delivery on pelvic floor function显示文摘Background Prolonged vaginal delivery times and episiotomy are associated with damage to the muscles of the pelvic floor,and with complications that include postpartum sexual dysfunction and urinary stress incontinence.This study was to compare the pelvic floor function among postpartum women who underwent three midwifery practices during labor:lateral episiotomy,traditional midwifery without episiotomy,and hands-off techniques delivery.Methods We included 320 primipara who were full-term pregnancy and underwent vaginal delivery in Department of Obstetrics,Peking University People’s Hospital,China.117 women had episiotomy during labor and were classified into the lateral episiotomy group;103 had traditional midwifery without episiotomy and were classified into the traditional midwifery practice group;and 100 experienced hands-off techniques during labor and were classified into the hands-off techniques delivery group.The duration of the second stage of labor,and postpartum pelvic floor muscle strength were compared between the three study groups.Results There were no significant differences between the three groups for the duration of the second stage of labor and postpartum pelvic floor muscle strength(P>0.05).However,the incidence of postpartum urinary stress incontinence and sexual dysfunction in the hands-off techniques delivery group was significantly lower than in the lateral episiotomy groups and the traditional midwifery practice group(P<0.01).Conclusions Hands-off techniques delivery could reduce damage to the pelvic floor and improve the quality of life for women after delivery. | Jingjing Gong Lili Xing Xiaodan Li Xiaona Wang Huijuan Chen | 2021 | Gynecology and Obstetrics Clinical Medicine2021，1，1： | 2 |
| 4 | Roadmap of environmental health research on emerging contaminants: Inspiration from the studies on engineered nanomaterials显示文摘Research on the environmental health of emerging contaminants is critical to understand their risks before causing severe harm.However,the low environmental concentrations,complex behaviors,and toxicology of emerging contaminants present enormous challenges for researchers.Here,we reviewed the research on the environmental health of engineered nanomaterials(ENMs),one of the typical emerging contaminants,to enlighten pathways for future research on emerging contaminants at their initial exploratory stage.To date,some developed pretreatment methods and detection technologies have been established for the determination of ENMs in natural environments.The mechanisms underlying the transfer and transformation of ENMs have been systematically explored in laboratory studies.The mechanisms of ENMs-induced toxicity have also been preliminarily clarified at genetic,cellular,individual,and short food chain levels,providing not only a theoretical basis for revealing the risk change and environmental health effects of ENMs in natural environments but also a methodological guidance for studying environmental health of other emerging contaminants.Nonetheless,due to the interaction of multiple environmental factors and the high diversity of organisms in natural environments,health effects observed in laboratory studies likely differ from those in natural environments.We propose a holistic approach and mesocosmic model ecosystems to systematically carry out environmental health research on emerging contaminants,obtaining data that determine the objectivity and accuracy of risk assessment. | Xiaona Li Feng He Zhenyu Wang Baoshan Xing | 2022 | Eco-Environment & Health2022，1，3： | 1 |
| 5 | Astragalus polysaccharide and oxymatrine can synergistically improve the immune efficacy of Newcastle disease vaccine in chicken显示文摘 | Yuku Chen Deyun Wang Yuanliang Hu Zhenhuan Guo Junmin Wang Xiaona Zhao Yunpeng Fan Liwei Guo Shujuan Yang Fuding Sai Yujuan Xing | 2010 | International Journal of Biological Macromolecules2010，，4： | 1 |
| 6 | Clarithromycin-loaded liposomes offering high drug loading and less irritation显示文摘 | Xiaona Liu Wei Sun Bo Zhang Bin Tian Xing Tang Na Qi Haibing He Huifang Li Xiangqun Jin | 2013 | International Journal of Pharmaceutics . 2013 (1-2)2013，，： | 1 |
| 7 | Exploration of the potential mechanism of Danggui Shaoyao powder in the treatment of endometriosis based on bioinformatics显示文摘Background:Endometriosis is a hormone-and organ-dependent disease with unclear pathogenesis.Danggui Shaoyao powder(DSP)has been used for the treatment of endometriosis for many years and has been proven to be effective,but its underlying molecular mechanisms remain unclear.In this study,we explored the molecular targets and pathways of DSP in the treatment of endometriosis mainly from the perspective of network pharmacology,and provided some novel ideas for managing this disease.Methods:In the present study,we focused on the underlying mechanisms between DPS and endometriosis,via oral bioavailability screening,drug similarity assessment,target identification,network analysis,drug relocation,and molecular docking using network pharmacology.Results:By bioinformatics,54 active components of DSP were predicted from the 534 components provided by the TCMSP database.We then found 85 validated targets and 6059 predicted targets for herbal components,and 2241 targets for endometriosis.After mapping,there were 255 targets in common.After that,network analysis was performed to screen key networks and core targets.Ninetynine indirect targets were collected from 14 direct ones.GO and KEGG analyses identified 63 enriched functions and 10 pathways(P<.01).According to the disease target,dipyridamole could undergo drug repositioning for endometriosis(score?1).The mechanism of action of dipyridamole on endometriosis was compared with that of DSP.Finally,eight targets,namely,AR,ESR1,HMGCR,NOS2,NR3C1,PPARG,PTGES,and PTGS2,were validated by molecular docking,suggesting that the probable molecular mechanisms by which DSP treats endometriosis are through seven validated pathways:hsa05200,hsa04915,hsa01100,hsa00900,hsa04920,hsa01130,and hsa00590.Conclusion:In this study,we uncovered the mechanism by which DSP can treat endometriosis using a drugecomponentetarget interaction network.This approach can provide some novel ideas for the development of Chinese medicine and the clinical management of endometriosis. | Xiuping Zhang Lulu Zhang Qi Wang Xinhui Sun Yang Dong Yu Xing Xiaona Ma | 2019 | Journal of Traditional Chinese Medical Sciences2019，6，4： | 1 |
| 8 | A new DNA vaccine fused with the C3d-p28 induces a Th2 immune response against amyloid-beta显示文摘To enhance anti-amyloid-beta(Aβ) antibody generation and induce a Th2 immune response,we constructed a new DNA vaccine p(Aβ3-10)10-C3d-p28.3 encoding ten repeats of Aβ3-10 and three copies of C3d-p28 as a molecular adjuvant.In this study,we administered this adjuvant intramuscularly to female C57BL/6J mice at 8-10 weeks of age.Enzyme linked immunosorbent assay was used to detect the titer of serum anti-Aβ antibody,isotypes,and cytokines in splenic T cells.A 3(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was used to detect the proliferation rate of splenic T cells.Brain sections from a 12-month-old APP/PS1 transgenic mouse were used for detecting the binding capacities of anti-Aβ antibodies to Aβ plaques.The p(Aβ3-10)10-C3d-p28.3 vaccine induced high titers of anti-amyloid-β antibodies,which bound to Aβ plaques in APP/PS1 transgenic mouse brain tissue,demonstrating that the vaccine is effective against plaques in a mouse model of Alzheimer's disease.Moreover,the vaccine elicited a predominantly IgG1 humoral response and low levels of interferon-γ in ex vivo cultured splenocytes,indicating that the vaccine could shift the cellular immune response towards a Th2 phenotype.This indicated that the vaccine did not elicit a detrimental immune response and had a favorable safety profile.Our results indicate that the p(Aβ3-10)10-C3d-p28.3 vaccine is a promising immunotherapeutic option for Aβ vaccination in Alzheimer's disease. | Wanshu Guo Sha Sha Tongzi Jiang Xiaona Xing Yunpeng Cao | 2013 | Neural Regeneration Research2013，8，27： | 0 |
| 9 | Assessment of the allergenicity and antigenicity potential of enzymatically hydrolyzed cow milk显示文摘Cow milk allergy is a major cause of food hypersensitivity in infants and children.However,to the best of our knowledge,there has been minimal research on the antigenicity of cow milk(CM).As it is currently unknown if enzymatically hydrolyzed cow milk(HM)induces severe immunogenic responses,the potential allergenicity of HM was systematically evaluated in this work.HM effectively attenuated symptoms of weight loss and diarrhea,suppressed levels of mast cell tryptase,and relieved allergic inflammation of the intestine.Moreover,HM reduced the B220+cell count in mouse blood.In addition,in vitro studies demonstrated that gastrointestinal digestion of HM was not resistant to pepsin or trypsin.Thus,HM effectively alleviated allergic reactions and exhibited lower potential allergenicity.These results provide practical information for the future development of hypoallergenic CM products. | Xiaona Liang Hui Yang Xue Luo Na Chen Zhisong Ai Yu Xing Wei Huang Zongzhou Wang Yan Zheng Xiqing Yue | 2022 | Food Bioscience2022，45，1： | 0 |
| 10 | Energy consumption and carbon emissions of hospitals in Tianjin显示文摘22 所第三级的医院的精力审计在天津被进行。详细内容包括了设计数据,基本信息,精力帐单和设备表。在学习期间,在天津的医院的精力紧张是近似稳定的,精力消费的平均水平是 348 kW,这被显示出吗?? | Chongxu JIANG Jincheng XING Jihon LING Xiaona QIN | 2012 | Frontiers in Energy2012，6，4： | 0 |
| 11 | Prediction of the Slope Solute Loss Based on BP Neural Network显示文摘The existence of soil macropores is a common phenomenon.Due to the existence of soil macropores,the amount of solute loss carried by water is deeply modified,which affects watershed hydrologic response.In this study,a new improved BP(Back Propagation)neural network method,using Levenberg–Marquand training algorithm,was used to analyze the solute loss on slopes taking into account the soil macropores.The rainfall intensity,duration,the slope,the characteristic scale of macropores and the adsorption coefficient of ions,are used as the variables of network input layer.The network middle layer is used as hidden layer,the number of hidden nodes is five,and a tangent transfer function is used as its neurons transfer function.The cumulative solute loss on the slope is used as the variable of network output layer.A linear transfer function is used as its neurons transfer function.Artificial rainfall simulation experiments are conducted in indoor experimental tanks in order to verify this model.The error analysis and the performance comparison between the proposed method and traditional gradient descent method are done.The results show that the convergence rate and the prediction accuracy of the proposed method are obviously higher than that of traditional gradient descent method.In addition,using the experimental data,the influence of soil macropores on slope solute loss has been further confirmed before the simulation. | Xiaona Zhang Jie Feng Zhiguo Yu Zhen Hong Xinge Yun | 2021 | Computers, Materials & Continua2021，，12： | 0 |
| 12 | Nasal mucosal inhalation of amyloid-beta peptide 3–10 defective adenovirus attenuates cytotoxicity induced by beta-amyloid(1–42)显示文摘Three-month-old Alzheimer's disease model transgenic mice were immunized with Aβ1–42, Plp-Adenovirus [Ad]-X-CMV-(Aβ3–10)10-CpG [AdCpG-(Aβ3–10)10] or AdCpG virus fluid via nasal mucosal inhalation, respectively. ELISA analysis of serum showed Aβ42 antibody titers were significantly increased in mice immunized with Aβ1–42 and AdCpG-(Aβ3–10)10. Concanavalin A and AdCpG-(Aβ3–10)10 stimulation significantly increased the number of proliferating spleen cells cultured from AdCpG(Aβ3–10)10 and Aβ42 groups compared with the control group. In the AdCpG(Aβ3–10)10 group, levels of interleukin(IL)-4 and IL-10 were increased, while those of IL-2 and interferon-γ were decreased. In the Aβ42 group, levels of IL-4, IL-10, IL-2 and interferon-γ were all increased. Experimental findings indicate that AdCpG-(Aβ3–10)10 vaccine can produce strong T helper 2(Th2) humoral immune responses in addition to the production of Aβ42 antibody. The cellular immunologic response was weak and avoided Aβ1–42-mediated cytotoxicity. | Tongzi Jiang Wanshu Guo Sha Sha Xiaona Xing Rong Guo Yunpeng Cao | 2014 | Neural Regeneration Research2014，9，8： | 0 |
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