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| 1 | Aerosolized STAT1 Antisense Oligodeoxynucleotides Decrease the Concentrations of Inflammatory Mediators in Bronchoalveolar Lavage Fluid in Bleomycin-Induced Rat Pulmonary Fibrosis显示文摘It has been demonstrated that alveolar macrophages (AMs) play a key role in the pathogenesis of pulmonary fibrosis by releasing a variety of cytokines and inflammatory mediators. In addition, abnormal signal transducer and activator of transcription-1 (STAT1) activation in AMs may play a pivotal role in the process of alveolitis and pulmonary fibrosis. In this study, we transfected STAT1 antisense oligodeoxynucleotide (ASON) into rats by aerosolization, and then investigated the effect of STAT1 ASON on inflammatory mediators such as TGF-β, PDGF and TNF-α in bronchoalveolar lavage fluid (BALF) from rats with bleomycin (BLM)-induced rat pulmonary fibrosis. Our results showed that STAT1 ASON by aerosolization could enter into lung tissues and AMs. STAT1 ASON could inhibit mRNA and protein expressions of STAT1 and ICAM-1 in AMs of rat with pulmonary fibrosis, and had no toxic side effect on liver and kidney. Aerosolized STAT1 ASON could ameliorate the alveolitis through inhibiting the secretion of inflammatory mediators in BLM-induced rat pulmonary fibrosis. These results suggest that aerosolized STAT1 ASON might be considered as a promising new strategy in the treatment of pulmonary fibrosis. | Ming Zeng Bin Liao Chen Zhu Wenjun Wang Xiaoqin Zhan Xianming Fan | 2008 | Cellular & Molecular Immunology2008，5，3： | 8 |
| 2 | Cloning and characterization of the gene encoding β-amyrin synthase in the glycyrrhizic acid biosynthetic pathway in Glycyrrhiza uralensis显示文摘Glycyrrhiza uralensis is considered to be one of the most important herbs in traditional Chinese medicine due to its numerous pharmacological effects particularly its ability to relieve cough and act as a mucolytic.Based on previous research,these effects are mediated by a number of active ingredients,especially glycyrrhizic acid(GA).In the present study,a gene encodingβ-amyrin synthase(β-AS)involved in GA biosynthesis in G.uralensis has been cloned and expressed in Saccharomyces cerevisiae.The cloned enzyme showed similar activity to native enzymes isolated from other Glycyrrhiza species to catalyze the conversion of 2,3-oxidosqualene intoβ-amyrin.In fact theβ-AS gene is particularly important in the GA biosynthetic pathway in G.uralensis.The complete sequence of the enzyme was determined and a phylogenetic tree based on theβ-AS gene of G.uralensis and 20 other species was created.This showed that Glycyrrhiza glabra had the closest kinship with G.uralensis.The results of this work will be useful in determining how to improve the efficacy of G.uralensis by improving its GA content and in exploring the biosynthesis of GA in vitro. | Honghao Chen Ying Liu Xiaoqin Zhang Xinjie Zhan Chunsheng Liu | 2013 | Acta Pharmaceutica Sinica B2013，3，6： | 5 |
| 3 | Effect of cathodic potential on stress corrosion cracking behavior of 21Cr2NiMo steel in simulated seawater显示文摘This study aims at providing systematically insights to clarify the impact of cathodic polarization on the stress corrosion cracking(SCC)behavior of 21 Cr2 NiMo steel.Slow-strain-rate tensile tests demonstrated that 21 Cr2 NiMo steel is highly sensitive to hydrogen embrittlement at strong cathodic polarization.The lowest SCC susceptibility occurred at-775 mV vs.SCE,whereas the SCC susceptibility was remarkably higher at potentials below-950 mV vs.SCE.Scanning electron microscopy(SEM)and electron backscattered diffraction(EBSD)revealed that the cathodic potential decline caused a transition from transgranular to intergranular mode in the fracture path.The intergranular mode transformed from bainite boundaries separation to prior austenitic grain boundaries separation under stronger cathodic polarization.Furthermore,corrosion pits promoted the nucleation of SCC cracks.In conclusion,with the decrease in the applied potential,the SCC mechanism transformed from the combination of hydrogen embrittlement and anodic dissolution to typical hydrogen embrittlement. | Menghao Liu Zhiyong Liu Cuiwei Du Xiaoqin Zhan Chunduo Dai Yue Pan Xiaogang Li | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，2： | 2 |
| 4 | An oral second-generation proteasome inhibitor oprozomib significantly inhibits lung cancer in a p53 independent manner in vitro显示文摘The destruction of proteins via the ubiquitin-proteasome system is amulti-step,complex process involving polyubiquitination of substrate proteins,follow ed by proteolytic degradation by the m acrom olecular 26S proteasome complex.Inhibitors of the proteasome prom ote the accum ulation of proteins that are deleterious to cell survival and are prom ising anticancer agents.Oprozomib(OPZ),an oral second-generation proteasome inhibitor,has been shown to inhibit the grow th of several cancers in preclinical and clinical trials,including m ultiple m yelom a and head and neck cancers,but its effects on lung cancer has not yet been determ ined.In this study,we evaluated the inhibitory effects of OPZ on lung cancer cell lines in vitro.The results showed that OPZ significantly suppressed cell proliferation and strongly induced apoptosis in both tested lung cancer cells independent of p53 expression.OPZ was able to cause obvious caspase 3 and PARP cleavages and stabilize p53 and its transcriptional targets p21,PUMA,and Noxa.Moreover,OPZ was capable of sensitizing lung cancer cells to the conventional chem otherapeutic drug cisplatin.Our study provides preclinical data and sheds light on the potential applications of proteasome inhibitor OPZ in lung cancer treatment. | Hongge Zhu Tianhai Wang Zhou Xin Yiyi Zhan Guoming Gu Xiaoqin Li Xiuli Wang Shune Yang Chunling Liu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，10： | 2 |
| 5 | Stress corrosion cracking behavior of high-strength mooring-chain steel in the SO_(2)-polluted coastal atmosphere显示文摘21Cr2NiMo steel is widely used to stabilize offshore oil platforms;however,it suffers from stress-corrosion cracking(SCC).Herein,we studied the SCC behavior of 21Cr2NiMo steel in SO_(2)-polluted coastal atmospheres.Electrochemical tests revealed that the addition of SO_(2) increased the corrosion current.Rust characterization showed that SO_(2) addition densified the corrosion products and promoted pitting.Furthermore,slow strain rate tests demonstrated a high susceptibility to SCC in high SO_(2) contents.Fracture morphologies revealed that the stress-corrosion cracks initiated at corrosion pits and the crack propagation showed transgranular and intergranular cracking modes.In conclusion,SCC is mix-controlled by anodic dissolution and hydrogen embrittlement mechanisms. | Menghao Liu Zhiyong Liu Cuiwei Du Xiaoqin Zhan Xiaojia Yang Xiaogang Li | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，6： | 1 |
| 6 | Dedifferentiated human umbilical cord mesenchymal stem cell reprogramming of endogenous hSDF-1a expression participates in neural restoration in hypoxic-ischemic brain damagerats显示文摘The transplantation of human umbilical cord mesenchymal stem cells(hUC-MSCs)can promote hypoxic-ischemic brain damage(HIBD)nerve repair,but finding suitable seed cells to optimize transplantation and improve treatment efficiency is an urgent problem to be solved.In this study,we induced hUC-MSCs into dedifferentiated hUC-MSCs(De-hUC-MSCs),and the morphology,stem cell surface markers,proliferation and tri-directional differentiation ability of the De-hUC-MSCs and hUC-MSCs were detected.A whole-gene chip was utilized for genome cluster,gene ontology and KEGG pathway analyses of differentially expressed genes.De-hUC-MSCs were transplanted into HIBD rats,and behavioral experiments and immunofluorescence assays were used to assess the therapeutic effect.A lentivirus vector for human stromal cell-derived factor-1(hSDF-1a)was constructed,and the role of hSDF-1a in the neuroprotective effect and mechanism of De-hUC-MSCs was verified.De-hUC-MSCs displayed similar cell morphology,stem cell surface marker expression,cell proliferation and even three-dimensional differentiation ability as hUC-MSCs but exhibited greater treatment potential in vivo.The reprogramming mechanism of hSDF-1a participated in the dedifferentiation process.By successfully constructing a stable hSDF-1a cell line,we found that De-hUC-MSCs might participate in nerve repair through the hSDF-1a/CXCR4/PI3K/Akt pathway.De-hUC-MSCs reprogramming of endogenous hSDF-1a expression may mediate the hSDF-1a/CXCR4/PI3K/Akt pathway involved in nerve repair in HIBD rats. | Zhou Xiaoqin Liu Jia Dai Mengjie Gu Jialu Bi Yang Wang Yuting Hu Huajian Liu Bo Zhang Xiaojun Li Zhongyue Chen Jie Li Tingyu Zhan Xue | 2021 | Genes & Diseases2021，8，3： | 1 |
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