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| 1 | Regulatory effect and mechanism of gastrin and its antagonists on colorectal carcinoma显示文摘AIM To explore the effect and mechanism ofgastrin and its antagonists proglumide and somatostatin on coIorectal carcinoma and their clinical significance.METHODS A model of transplanted human colonic carcinoma was established from SW480cell line in gymnomouse body. The volume andweight of transplanted carcinoma was observedunder the effect of pentagatrin (PG), proglumide (PGL) and octapeptide somotostatin (SMS201-995, SMS). The cAMP content ot carcinoma cell was determined by redioimmunoassay and the DNA, protein content and cell cycle were determined by flow-cytometry. The amount of viabIe cells was determined by MTT colorimetric analysis, lP3 content was determined by radioimmunoassay, Ca2+ concentration in cell by fluorometry and PKC activity by isotopic enzymolysis. The expression of gastrin, c-myc,C-fos and rasP21 in 48 cases of colorectal carcinoma tissue was detected by the immunocytochemistry SP method. Argyrophilianucleolar organizer regions was determined withargyrophilia stain.RESULTS The volume, weight, cAMP, DNAand protein content in carcinoma cell, cellamount and proliferation index of S and G2Mphase in PG group were all significantly higher than those of control group. When PG was at theconcentration of 25 mg/ L, the amount of viablecells, lP3 content and Ca2+ concentration in celland membrane PKC activity in PG group weresigniticantly higher than those in control group;when PGL was at a concentration ot 32 mg/L,they dropped to the lowest level in PG (25 mg/L)+ PGL group, but without significant differencefrom the control group. The positive expression rate of gastrin, c-myc, c-fos and rasp21 in carcinoma tissue was 39 .6%, 54 .2%, 47. 9% and54 .2% resPectively and significantly higher than that in mucosa 3 cm and 6 cm adjacent tocarcinoma tissue and normal colorectal mucosa.The positive expression rate of gastrin of highlydifferentiated adenocarcinoma group was significantly higher than that of poorly differentiated and mucinous adenocarcinoma groups. The AgNORs count of carcinoma tissue was significantly higher than that in mucosa 3 cm and 6 cm adjacent to carcinoma tissue and normal colorectal mucosa; and the positive expression of c-myc and c-fos and the AgNORs count in gastrin-positive group was significantly higher than those in gastrin-negative group.CONCLUSION Pentagastrin has a promoting effect on the growth of transplanted human colonic carcinoma from SW480 cell line. PGL hasno obvious effect on the growth of human colonic carcinoma SW480 cell line, but couldinhibit the growth-promoting effect of PG on transplanted carcinoma. Somatostatin can not only inhibit the growth of transplanted human colonic carcinoma from SW480 cell line directlybut also depress the growth-promoting effect ofgastrin on the transplanted carcinoma. Somecolorectal carcinoma cells can produce and secrete gastrin through autocrine, highly-differentiated adenocarcinoma express the highest level gastrin. Endogenous gastrin can stimulate the cell division and proliferation of carcinoma cell and promote the growth of colorectal carcinoma regulating the expression of oncogene omyc, c-fos. Our study has provided experimental basis for the adjuvant treatment using gastrin antagonist such as PGL,somatostatin of patients with colorectalcarcinoma. | He SW Shen KQ He YJ Xie B Zhao YM | 1999 | World Journal of Gastroenterology1999，5，5： | 20 |
| 2 | In vitro synergistic cytotoxiceffect of triptolide combined with hydroxycamptothecin on pancre-atic cancer cells 显示文摘 | Yang SW Wang W Xie XY | 2011 | Am J Chin Med2011，39，1： | 1 |
| 3 | RNA silencing genes control de novo DNA methylation显示文摘 | Chan SW Zilberman D Xie Z | 2004 | Science2004，303，5662： | 1 |
| 4 | Targeting hedgehog signaling in cancer: research and clinical developments 显示文摘 | Xie J Barrels CM Barton SW | 2013 | Onco Targets Ther2013，6，： | 1 |
| 5 | Co-expression of vascular endothelial growth factor(VEGF) and its receptors (flk-1 and flt-1) in hormone-induced mammary cancer in the Noble rat 显示文摘 | Tam NNC Tsao SW | 1999 | British J Cancer1999，81，8： | 1 |
| 6 | Cardioprotection by Klotho through downregulation of TRPC6 channels in the mouse heart 显示文摘 | Xie 1 Cha SK An SW | 2012 | Nat Commun2012，3，： | 1 |
| 7 | RNA silencing genes control de novo DNA methylation显示文摘 | Chan SW Zilberman D Xie Z | 2004 | Science2004，303，5662： | 1 |
| 8 | Characterization of Bot- ryosphaeriaceae dothidea, the causal agent of grapevine canker in china 显示文摘 | Yan JY Xie Y Yao SW | 2012 | Australasian Plant Pathol2012，，41： | 1 |
| 9 | Cardioprotection by Klotho through downregulation of TRPC6 channels in the mouse heart显示文摘 | Xie J Cha SK An SW | 2012 | Natural Communication2012，3，： | 1 |
| 10 | Induction of high incidence of mam- mary tumour in female Noble rats with a combination of 17beta- oestradiol and testosterone显示文摘 | Xie B Tsao SW Wong YC | 1999 | Carcinogenesis1999，20，6： | 1 |
| 11 | Contrast-enhanced ultrasonography with contrast-tuned imaging technology for the detection of prostate cancer:comparison with conventional ultrasonography显示文摘 | Xie SW Li HL Du J | 2012 | BJU Int2012，109，11： | 1 |
| 12 | Origin of Tibeto-Bumlan speakers: Evidence from HLA allele distribution in Lisu and Nu inhabiting Yunnan of China显示文摘 | Chen SW Hu QS Xie Y | 2007 | Hum Immunol2007，68，： | 1 |
| 13 | Value of contrast-enhanced sonographic micro flow imaging for prostate cancer detection with t-PSA level of 4-10 ng/ml显示文摘 | Guo YF Li FH Xie SW | 2012 | Eur J Radiol2012，81，11： | 1 |
| 14 | Targeting hedgehog signaling in cancer research and clinical developments 显示文摘 | Xie J Bartels CM Barton SW | 2013 | Onco Targets Ther2013，6，： | 1 |
| 15 | Ghrelin Reduces Injury of Hippocampal Neurons in a Rat Model of Cerebral Ischemia/Reperfusion显示文摘 | Yajun Liu Paulus SW Dongping Xie | 2006 | Chinese Journal of Physiology2006，49，5： | 1 |
| 16 | 显示文摘 | | 1999 | Carcinogenesis,1999,20(8):1069-1 0781999，20，8： | 1 |
| 17 | Influence of serum prostate-specific antigen (PSA) level, prostate volume, and PSA density on prostate cancer detection with contrast-enhanced sonography using contrast-tuned imaging technology显示文摘 | Xie SW Li HL Du J | 2013 | J Ultrasound Med2013，32，： | 1 |
| 18 | Soluble Klotho Protects against Uremic Cardiomyopathy Independently of Fibroblast Growth Factor 23 and Phosphate显示文摘 | XIE J YOON J AN SW | 2015 | J Am Soc Nephrol2015，26，5： | 1 |
| 19 | Value of contrast-enhanced sonographic micro flow imaging for prostate cancer detection with t-PSA level of 4-10 ng/mL显示文摘 | Guo YF Li FH Xie SW | 2012 | Eur J Radiol2012，81，： | 1 |
| 20 | Influence of serum prostate - specific antigen (PSA) level, prostate volume, and PSA density on pros- tate cancer detection with contrast - enhanced sonography using contrast - tuned imaging technology 显示文摘 | Xie SW Li HL Du J | 2013 | Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine2013，32，5： | 1 |
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