|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids显示文摘Magnolia biondii Pamp.(Magnoliaceae,magnoliids)is a phylogenetically,economically,and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China.Determining the genome sequence of M.biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus.We assembled a chromosome-level reference genome of M.biondii using~67,~175,and~154Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing,10X Genomics Chromium,and Hi-C scaffolding strategies,respectively.The final genome assembly was~2.22Gb,with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%.Approximately 89.17%of the genome was organized into 19 chromosomes,resulting in a scaffold N50 of 92.86Mb.The genome contained 47,547 protein-coding genes,accounting for 23.47%of the genome length,whereas 66.48%of the genome length consisted of repetitive elements.We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales.Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites,plant–pathogen interactions,and responses to stimuli,which may improve the ecological fitness and biological adaptability of the lineage.Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae,which are sister to a clade comprising monocots and eudicots.The genome sequence of M.biondii could lead to trait improvement,germplasm conservation,and evolutionary studies on the rapid radiation of early angiosperms. | Shanshan Dong Min Liu Yang Liu Fei Chen Ting Yang Lu Chen Xingtan Zhang Xing Guo Dongming Fang Linzhou Li Tian Deng Zhangxiu Yao Xiaoan Lang Yiqing Gong Ernest Wu Yaling Wang Yamei Shen Xun Gong Huan Liu Shouzhou Zhang | 2021 | Horticulture Research2021，8，1： | 4 |
| 2 | Theories and methods of designing microdisplacement actuator based on giant materials显示文摘 | JIA Zhenyuan YANG Xing GUO Dongming | 1996 | Chinese Journal of Mechanical Engineering1996，19，3： | 1 |
| 3 | Alkaloids from Portulaca oleracea L显示文摘 | Lan Xiang Dongming Xing Wei Wang | 2005 | Phytochemistry2005，66，21： | 1 |
| 4 | Kinetic difference of berberine between bippocampus and plasma in rat after intravenous administration of Coptidis Rhizoma extract 显示文摘 | Xueli Wang Rufeng Wang Dongming Xing | 2005 | Life Sciences2005，77，： | 1 |
| 5 | HPLC method for the determination and pharmacokinetic studies on puerarin in cerebral ischemia reperfusion rat plasma after intravenous administration of puerariae radix isoflavone 显示文摘 | Bin Yan Dongming Xing Yi Ding | 2005 | J Pharm Bio Analysis2005，37，2： | 1 |
| 6 | Kinet-ic difference of berberine between hippocampus and plasma in rat after intravenous administration of Coptidis rhizoma extract显示文摘 | Rufeng Wang Dongming Xing | 2005 | Life Sciences2005，77，： | 1 |
| 7 | Unequal loss protection for FGS video transmission under bandwidth constrained channel 显示文摘 | Sun Gang Xing Wei Lu Dongming | 2006 | Journal of Zhejiang University: Engineering Science2006，40，12： | 1 |
| 8 | Ischemic Postconditioning Inhibits Apoptosis After Focal Cerebral Ischemia/Reperfusion Injury in the Rat显示文摘 | Bianzhi Xing Hui Chen Min Zhang Dongming Zhao Rui Jiang Xiuheng Liu Suming Zhang | 2008 | Stroke2008，，8： | 1 |
| 9 | Effects of cerebral ischemia-reperfusion on pharmacokinetic fate of paeoniflorin after intravenous administration of Paeoniae Radix extract in rats显示文摘 | He Xihui Xing Dongming Ding Yi | 2004 | J Ethnopharmacol2004，94，： | 1 |
| 10 | Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract显示文摘 | Xihui He Dongming Xing Yi Ding | 2004 | Journal of Chromatography B2004，820，2： | 1 |
| 11 | PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis显示文摘 | Weiwei Yang Yan Xia David Hawke Xinjian Li Ji Liang Dongming Xing Kenneth Aldape Tony Hunter W.K. Alfred Yung Zhimin Lu | 2012 | Cell2012，，4： | 1 |
| 12 | Determination of paeoniflorin in rat hippocampus by high-performance liquid chromatography after intravenous administration of Paeoniae Radix extract显示文摘 | Dongming Xing Yi Ding | 2004 | Journal of Chromatography B2004，820，2： | 1 |
| 13 | AstragalosideⅣameliorates insulin induced insulin resistance in HepG2 cells through reactive oxygen species mediated c-Jun N-terminal kinase pathway显示文摘OBJECTIVE:To investigate the effects and elucidate the mechanism of Astragaloside IV(AS-IV)for insulin resistance(IR)and type 2 diabet es mellitus(T2DM).METHODS:CCK8 kit was used to detect cell viability,glucose detection kit was used to detect the concentration of glucose in cell supernatant,reactive oxygen species(ROS)detection kit and Western blot were used to explore the mechanism of Astragaloside IV(AS-IV)in improving IR.A diabetic rat model was also established by feeding high sugar and fat diet and streptozotocin(STZ)injection.After treatment with AS-IV,rosiglitazone(ROZ),or normal saline,the fasting blood glucose(FBG),C peptide(C-P),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and the glucose tolerance were assessed.RESULTS:AS-IV could effectively reduce the content of ROS and increase the glucose uptake in high insulintreated IR-type HepG 2 cells.The results of molecular mechanisms indicated that AS-IV could improve insulin resistance by reducing JNK phosphorylation and regulating c-Jun N-terminal kinase(JNK)downstream protein expression.Additionally,AS-IV could significantly reduce the levels of FBG,TNF-α,IL-6 and the glucose tolerance in diabetic rats(P<0.05 or<0.01).The high and medium dose groups of AS-IV could significantly increase the C-P levels in diabetic rats(P<0.05 or<0.01).CONCLUSIONS:Our results indicated that AS-IV improve liver IR through the JNK pathway and ROS,which meant a new molecular target for the treatment of diabetes.The AS-IV also helped to prevent and improved the insulin resistance of rats. | YE Xiaomei XING Xiaowei YUAN Kangrui WANG Dongming WU Dudu CHEN Zhi YU Zhiqiang | 2023 | Journal of Traditional Chinese Medicine2023，43，1： | 1 |
| 14 | Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549显示文摘 | Xiaolu Yin Jiangbing Zhou Chunfa Jie Dongming Xing Ying Zhang | 2004 | Life Sciences2004，，： | 1 |
| 15 | Advances and challenges in using nirmatrelvir and its derivatives against SARS-CoV-2 infection显示文摘On December 22,2021,the United States Food and Drug Administration approved the first main protease inhibitor,i.e.,oral antiviral nirmatrelvir(PF-07321332)/ritonavir(Paxlovid),for the treatment of early severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.Nirmatrelvir inhibits SARSCoV-2 infection,but high doses or long-term treatment may cause embryonic developmental toxicity and changes in host gene expression.The chiral structure of nirmatrelvir plays a key role in its antiviral activity.Ritonavir boosts the efficacy of nirmatrelvir by inactivating cytochrome P4503A4 expression and occupying the plasma protein binding sites.Multidrug resistance protein 1 inhibitors may increase the efficacy of nirmatrelvir.However,Paxlovid has many contraindications.Some patients treated with Paxlovid experience a second round of coronavirus disease 2019(COVID-19)symptoms soon after recovery.Interestingly,the antiviral activity of nirmatrelvir metabolites,such as compounds 12e18,is similar to or higher than that of nirmatrelvir.Herein,we review the advances and challenges in using nirmatrelvir and its derivatives with the aim of providing knowledge for drug developers and physicians in the fight against COVID-19. | Wujun Chen Bing Liang Xiaolin Wu Ling Li Chao Wang Dongming Xing | 2023 | Journal of Pharmaceutical Analysis2023，13，3： | 1 |
| 16 | Alkaloids from Portulaca oleracea L 显示文摘 | Lan Xiang Dongming Xing Wei Wang | 2005 | Phytochemistry2005，66，： | 1 |
| 17 | New insights into the suppression of inflammation and lipid accumulation by JAZF1显示文摘Atherosclerosis is one of the leading causes of disease and death worldwide.The identification of new therapeutic targets and agents is critical.JAZF1 is expressed in many tissues and is found at particularly high levels in adipose tissue(AT).JAZF1 suppresses inflammation(including IL-1β,IL-4,IL-6,IL-8,IL-10,TNFα,IFN-γ,IAR-20,COL3A1,laminin,and MCP-1)by reducing NF-κB pathway activation and AT immune cell infiltration.JAZF1 reduces lipid accumulation by regulating the liver X receptor response element(LXRE)of the SREBP-1c promoter,the cAMP-response element(CRE)of HMGCR,and the TR4 axis.LXRE and CRE sites are present in many cytokine and lipid metabolism gene promoters,which suggests that JAZF1 regulates these genes through these sites.NF-κB is the center of the JAZF1-mediated inhibition of the inflammatory response.JAZF1 suppresses NF-κB expression by suppressing TAK1 expression.Interestingly,TAK1 inhibition also decreases lipid accumulation.A dual-targeting strategy of NF-κB and TAK1 could inhibit both inflammation and lipid accumulation.Dual-target compounds(including prodrugs)1–5 exhibit nanomolar inhibition by targeting NF-κB and TAK1,EGFR,or COX-2.However,the NF-κB suppressing activity of these compounds is relatively low(IC50>300 nM).Compounds 6–14 suppress NF-κB expression with IC50 values ranging from 1.8 nM to 38.6 nM.HS-276 is a highly selective,orally bioavailable TAK1 inhibitor.Combined structural modifications of compounds using a prodrug strategy may enhance NF-κB inhibition.This review focused on the role and mechanism of JAZF1 in inflammation and lipid accumulation for the identification of new anti-atherosclerotic targets. | Wujun Chen Yingjie Zhong Yang Yuan Meng Zhu Wenchao Hu Ning Liu Dongming Xing | 2023 | Genes & Diseases2023，10，6： | 0 |
| 18 | Epigenome-wide development and validation of a prognostic methylation score in intrahepatic cholangiocarcinoma based on machine learning strategies显示文摘Background:Clinical parameter-based nomograms and staging systems provide limited information for the prediction of survival in intrahepatic cholangiocarcinoma(ICC)patients.In this study,we developed a methylation signature that precisely predicts overall survival(OS)after surgery.Methods:An epigenome-wide study of DNA methylation based on whole-genome bisulfite sequencing(WGBS)was conducted for two independent cohorts(discovery cohort,n=164;validation cohort,n=170)from three hepatobiliary centers in China.By referring to differentially methylated regions(DMRs),we proposed the concept of prognostically methylated regions(PMRs),which were composed of consecutive prognostically methylated CpGs(PMCs).Using machine learning strategies(Random Forest and the least absolute shrinkage and selector regression),a prognostic methylation score(PMS)was constructed based on 14 PMRs in the discovery cohort and confirmed in the validation cohort.Results:The C-indices of the PMS for predicting OS in the discovery and validation cohorts were 0.79 and 0.74,respectively.In the whole cohort,the PMS was an independent predictor of OS[hazard ratio(HR)=8.12;95% confidence interval(CI):5.48-12.04;P<0.001],and the C-index(0.78)of the PMS was significantly higher than that of the Johns Hopkins University School of Medicine(JHUSM)nomogram(0.69,P<0.001),the Eastern Hepatobiliary Surgery Hospital(EHBSH)nomogram(0.67,P<0.001),American Joint Committee on Cancer(AJCC)tumor-node-metastasis(TNM)staging system(0.61,P<0.001),and MEGNA prognostic score(0.60,P<0.001).The patients in quartile 4 of PMS could benefit from adjuvant therapy(AT)(HR=0.54;95%CI:0.32-0.91;log-rank P=0.043),whereas those in the quartiles 1-3 could not.However,other nomograms and staging system failed to do so.Further analyses of potential mechanisms showed that the PMS was associated with tumor biological behaviors,pathway activation,and immune microenvironment.Conclusions:The PMS could improve the prognostic accuracy and identify patients who would benefit from AT for ICC patients,and might facilitate decisions in treatment of ICC patients. | Xing Chen Liangqing Dong Lu Chen Yuan Wang Jinpeng Du Lijie Ma Xiaokai Yan Jiwei Huang Mingheng Liao Xiangzheng Chen Dongming Liu Jin Li Bo Zhang Wen Teng Kefei Yuan Deqiang Sun Qiang Gao Yong Zeng | 2023 | Hepatobiliary Surgery and Nutrition2023，12，4： | 0 |
| 19 | An Empirical Study on Organizational Efficiency of Drug Review Institutions显示文摘Objective To construct the influencing factor model of organizational efficiency by using structural equation model,and to put forward some suggestions for drug review institutions to their management mode.Methods The model hypothesis affecting organizational efficiency was proposed by literature analysis,and a questionnaire was designed.Then,the questionnaires returned were analyzed to investigate the relationship among the factors affecting organizational efficiency with the structural equation models.Results and Conclusion The direct effect of communication on organizational efficiency was 0.83,that of system construction was 0.60,talents cultivation was 0.25,and task management was 0.38.The model results basically fitting met the various statistical indicators,with statistical significance thresholds.Talents cultivation and task management have little effect on organizational efficiency,while system construction and communication have great effect on organizational efficiency.However,due to the correlation between organizational systems,the influencing factors should be considered in a balanced manner,so as to put forward reasonable suggestions. | Wu Dongming Jia Zheng Wang Lifei Xing Hua | 2023 | Asian Journal of Social Pharmacy2023，18，3： | 0 |
| 20 | Nanozyme-activating prodrug therapies:A review显示文摘Enzyme prodrug therapies(EPTs)have been intensively explored as attractive approaches to selective activation of systemically administered benign prodrugs by the exogenous enzymes or enzymes expressed at the desired target site,thus achieving localized,site-specific therapeutic effect.Many effective strategies(e.g.,antibody-,viral-,gene-,as well as polymer-directed EPT)have been developed for enzyme localization to locally activate systemically administered benign prodrugs.Nevertheless,intrinsic limitations(e.g.,complex intracellular environment and catalyst instability)make the practical application of EPT strategies a task that presents itself as highly challenging.As a promising alternative to natural enzyme,nanozyme has attracted substantial attention since its discovery in 2007,mainly due to the advantages of robust catalytic activity,high stability,low cost,and facile synthesis.Recently,nanozyme-activated prodrug strategies bring a new opportunity for targeted therapy,referred to as nanozyme-activating prodrug therapies.This review focuses on recently reported nanozyme-activated prodrug strategies,aiming to provide some new insights into the potential applications in site-specific drug synthesis. | Yudong Wu Wujun Chen Chao Wang Dongming Xing | 2024 | Chinese Chemical Letters2024，35，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员