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| 1 | Endometrial membrane organoids from human embryonic stem cell combined with the 3D Matrigel for endometrium regeneration in asherman syndrome显示文摘Asherman’s syndrome(AS),a leading cause of uterine infertility worldwide,is characterized by scarring of the uterine surfaces lacking endometrial epithelial cells,which prevents endometrial regeneration.Current research on cell therapy for AS focuses on mesenchymal and adult stem cells from the endometrium.However,insufficient number,lack of purity,and rapid senescence of endometrial epithelial progenitor cells(EEPCs)during experimental processes restrict their use in cell therapies.In this study,we induced human embryonic stem cells-9(H9-ESC)into EEPCs by optimizing the induction factors from the definitive endoderm.EEPCs,which act as endometrial epithelial cells,accompanied by human endometrial stromal cells provide a niche environment for the development of endometrial membrane organoids(EMOs)in an in vitro 3D culture model.To investigate the function of EMOs,we transplanted tissue-engineered constructs with EMOs into an in vivo rat AS model.The implantation of EMOs into the damaged endometrium facilitates endometrial regeneration and angiogenesis.Implanting EMOs developed from human embryonic stem cells into the endometrium might prove useful for“endometrial re-engineering”in the treatment of Asherman’s syndrome. | Xiuxiu Jiang Xingmiao Li Xiangwei Fei Jiajie Shen Jianhua Chen Meijun Guo Yangyang Li | 2021 | Bioactive Materials2021，6，11： | 11 |
| 2 | Identification of SNPs in barley(Hordeum vulgare L.)by deep sequencing of six reduced representation libraries显示文摘High-density genetic markers are required for genotyping and linkage mapping in identifying genes from crops with complex genomes, such as barley. As the most common variation, single nucleotide polymorphisms(SNPs) are suitable for accurate genotyping by using the next-generation sequencing(NGS) technology. Reduced representation libraries(RRLs) of five barley accessions and one mutant were sequenced using NGS technology for SNP discovery. Twenty million short reads were generated and the proportion of repetitive sequences was reduced by more than 56%. A total of 6061 SNPs were identified, and 451 were mapped to the draft sequence of the barley genome with pairing reads. Eleven SNPs were validated using length polymorphic allele-specific PCR markers. | Ganggang Guo Dawa Dondup Lisha Zhang Sha Hu Xingmiao Yuan Jing Zhang | 2014 | The Crop Journal2014，2，6： | 4 |
| 3 | Fast mapping of a chlorophyll b synthesis-deficiency gene in barley(Hordeum vulgare L.) via bulked-segregant analysis with reduced-representation sequencing显示文摘Bulked-segregant analysis coupled with next-generation sequencing(BSA-seq) has emerged as an efficient tool for genetic mapping of single genes or major quantitative trait loci controlling(agronomic) traits of interest. However, such a mapping-by-sequencing approach usually relies on deep sequencing and advanced statistical methods. Application of BSA-Seq based on construction of reduced-representation libraries and allele frequency analysis permitted anchoring the barley pale-green(pg) gene on chromosome 3 HL. With further marker-assisted validation, pg was mapped to a 3.9 Mb physical-map interval. In the pg mutant a complete deletion of chlorophyllide a oxygenase(HvCAO) gene was identified.Because the product of this gene converts Chl a to Chl b, the pg mutant is deficient in Chl b.An independent Chl b-less mutant line M4437_2 carried a nonsynonymous substitution(F263 L) in the C domain of HvCAO. The study demonstrates an optimized pooling strategy for fast mapping of agronomically important genes using a segregating population. | Dongdong Xu Dan Sun Yanling Diao Minxuan Liu Jia Gao Bin Wu Xingmiao Yuan Ping Lu Zongwen Zhang Jing Zhang Ganggang Guo | 2019 | The Crop Journal2019，7，1： | 3 |
| 4 | SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by Repressing PTP1B显示文摘 | Cheng Sun Fang Zhang Xinjian Ge Tingting Yan Xingmiao Chen Xianglin Shi Qiwei Zhai | 2007 | Cell Metabolism2007，，4： | 3 |
| 5 | SIRT1 Improves Insulin Sensitivity under Insulin - Resistant Conditions by Re- pressing PTP1 B 显示文摘 | Cheng Sun Fang Zhang Xinjian Ge Tingting Yan Xingmiao Chen Xianglin Shi and Qiwei Zha | 2007 | Cell Metabolism2007，6，： | 1 |
| 6 | Synthesis and photocatalytic activity of graphene/BiOBr composites under visible light显示文摘 | Xingmiao Zhang Xiaofeng Chang M.A. Gondal Bin Zhang Yousong Liu Guangbin Ji | 2012 | Applied Surface Science2012，，20： | 1 |
| 7 | μ-Syntheses of Suspension Systems for EMS and EDS Maglev Vehicles显示文摘 | Zhang Hanquan Yao Xingmiao Ji Zhenhai | 2003 | Journal of Southwest Jiaotong University (S0054-2429)2003，11，1： | 1 |
| 8 | SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by Repressing PTP1B显示文摘 | Cheng Sun Fang Zhang Xinjian Ge Tingting Yan Xingmiao Chen Xianglin Shi Qiwei Zhai | 2007 | Cell Metabolism2007，，4： | 1 |
| 9 | Syn- thesis and photocatalytic activity of graphene/BiOBr com- posites under visible light显示文摘 | Zhang Xingmiao Chang Xiaofeng Gondal M A | 2012 | Appl Surf Sci2012，258，： | 1 |
| 10 | Synthesis and photocatalytic activity of graphene/BiOBr composites under visible light显示文摘 | Xingmiao Zhang Xiaofeng Chang M.A. Gondal Bin Zhang Yousong Liu Guangbin Ji | 2012 | Applied Surface Science2012，，20： | 1 |
| 11 | Semi-supervised Internetnetwork traffic classification using a Gaussian mixture model 显示文摘 | Qian Feng Hu Guangmin Yao Xingmiao | 2008 | AEU-lnternationai Journal of Electronics and Communications2008，62，7： | 1 |
| 12 | RUAP:Random Rearrangement Block Matrix-Based Ultra-Lightweight RFID Authentication Protocol for End-Edge-Cloud Collaborative Environment显示文摘Cloud computing provides powerful processing capabilities for large-scale intelligent Internet of things(IoT)terminals.However,the massive realtime data processing requirements challenge the existing cloud computing model.The edge server is closer to the data source.The end-edge-cloud collaboration offloads the cloud computing tasks to the edge environment,which solves the shortcomings of the cloud in resource storage,computing performance,and energy consumption.IoT terminals and sensors have caused security and privacy challenges due to resource constraints and exponential growth.As the key technology of IoT,Radio-Frequency Identification(RFID)authentication protocol tremendously strengthens privacy protection and improves IoT security.However,it inevitably increases system overhead while improving security,which is a major blow to low-cost RFID tags.The existing RFID authentication protocols are difficult to balance overhead and security.This paper designs an ultra-lightweight encryption function and proposes an RFID authentication scheme based on this function for the end-edge-cloud collaborative environment.The BAN logic proof and protocol verification tools AVISPA formally verify the protocol’s security.We use VIVADO to implement the encryption function and tag’s overhead on the FPGA platform.Performance evaluation indicates that the proposed protocol balances low computing costs and high-security requirements. | Yu Luo Kai Fan Xingmiao Wang Hui Li Yintang Yang | 2022 | China Communications2022，19，7： | 0 |
| 13 | Immunocytochemical identification of actin in mitochdria of Physarum polycephalum显示文摘Mitochondria isolated from the plasmodia of Physarum polycephalum Schw.are reacted with rabbit anti-actin antibody,and detected with FITC-conjugated sheep anti-rabbit IgG antibody.The results of indirect immunofluorescence show that actin exists in the mitochondria.Western blot analysis confirms the existence of actin in the protein preparation of the mitochondria.The indirect immunoelectron microscopic observation using the same antibodies verifies further that actin is the constituents of mitochondria,and it is dispersively distributed in the mitochondria of P.polycephalum. | ZENGXianlu XINGMiao WANGXiaoguang SONGZhaoxia HAOShui | 2003 | Progress in Natural Science:Materials International2003，13，1： | 0 |
| 14 | C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting-1 PRF and the NS3 Protein显示文摘The Japanese encephalitis serogroup of the neurogenic Flavivirus has a specific feature that expresses a non-structural protein NS1'produced through a programmed-1 ribosomal frameshifting(-1 PRF).Herein,C19orf66,a novel member of interferon-stimulated gene(ISG)products,exhibited significant activity of antagonizing Japanese encephalitis virus(JEV)infection.Overexpression of C19orf66 in 293T cells significantly inhibited JEV replication,while knock-down of endogenous C19orf66 in HeLa cells and A549 cells significantly increased virus replication.Notably,C19orf66 had an inhibitory effect on frameshift production of JEV NS1'.The inhibition was more significant when C19orf66 and JEV NS1-NS2A were co-expressed in the 293T cells.Both C19orf66-209 and C19orf66-Zinc^(mut) did not significantly change the NS1'to NS1 ratio and had weaker antiviral effects than C19orf66.Similarly,C19orf66-209 and C19orf66-Zinc^(mut) had no significant effect on the expression of the JEV NS3 protein,whose expression was down-regulated by C19orf66 via the lysosome-dependent pathway.These findings suggest that C19orf66 may possess at least two different mechanisms of antagonizing JEV infection.This study identified C19orf66 as a novel interferon-stimulated gene product that can inhibit JEV replication by targeting-1 PRF and the NS3 protein.The study provides baseline information for the future development of broad-spectrum antiviral agents against JEV. | Du Yu Yundi Zhao Junhui Pan Xingmiao Yang Zhenjie Liang Shengda Xie Ruibing Cao | 2021 | Virologica Sinica2021，36，6： | 0 |
| 15 | Japanese Encephalitis Virus NS2B-3 Protein Complex Promotes Cell Apoptosis and Viral Particle Release by Down-Regulating the Expression of AXL显示文摘Japanese encephalitis virus(JEV) is a flavivirus transmitted by mosquitoes that causes severe encephalitis in humans and animals. It has been suggested that AXL, a transmembrane protein, can promote the replication of various flaviviruses,such as dengue(DENV), Zika(ZIKV), and West Nile(WNV) viruses. However, the effect of AXL on JEV infection has not yet been determined. In the present study, we demonstrate that AXL is down-regulated after JEV infection in the late stage. JEV NS2B-3 protein specifically interacted with AXL, and promoted AXL degradation through the ubiquitin–proteasome pathway. AXL-degradation increased cell apoptosis by disrupting phosphatidylinositol 3-kinase(PI3 K)/Akt signal transduction. In addition, the degradation of AXL promoted JEV release to supernatant, whereas the virus in the cell lysates decreased. The supplementation of AXL ligand Gas6 inhibited the JEV-mediated degradation of AXL. Altogether,we discover a new function of NS2B-3 during the process of JEV replication, and provide a new insight into the interactions between JEV and cell hosts. | Shengda Xie Zhenjie Liang Xingmiao Yang Junhui Pan Du Yu Tongtong Li Ruibing Cao | 2021 | Virologica Sinica2021，36，6： | 0 |
| 16 | APPL2 Negatively Regulates Olfactory Functions by Switching Fate Commitments of Neural Stem Cells in Adult Olfactory Bulb via Interaction with Notch1 Signaling显示文摘Adult olfactory neurogenesis plays critical roles in maintaining olfactory functions.Newly-generated neurons in the subventricular zone migrate to the olfactory bulb(OB) and determine olfactory discrimination,but the mechanisms underlying the regulation of olfactory neurogenesis remain unclear.Our previous study indicated the potential of APPL2(adaptor protein,phosphotyrosine interacting with PH domain and leucine zipper 2) as a modulating factor for neurogenesis in the adult olfactory system.In the present study,we report how APPL2 affects neurogenesis in the OB and thereby mediates olfactory discrimination by using both in vitro neural stem cells(NSCs) and an in vivo animal model-APPL2 transgenic(Tg) mice.In the in vitro study,we found that APPL2 overexpression resulted in NSCs switching from neuronal differentiation to gliogenesis while APPL2 knockdown promoted neurogenesis.In the in vivo study,APPL2 Tg mice had a higher population of glial cells and dampened neuronal production in the olfactory system,including the corpus callosum,OB,and rostral migratory stream.Adult APPL2 Tg mice displayed impaired performance in olfactory discrimination tests compared with wild-type mice.Furthermore,we found that an interaction of APPL2 with Notch1 contributed to the roles of APPL2 in modulating the neurogenic lineage-switching and olfactory behaviors.In conclusion,APPL2 controls olfactory discrimination by switching the fate choice of NSCs via interaction with Notch1 signaling. | Chong Gao Tingting Yan Xingmiao Chen Kenneth K.Y.Cheng Aimin Xu Jiangang Shen | 2020 | Neuroscience Bulletin2020，36，9： | 0 |
| 17 | 用于精确调控单层二氧化钛介孔涂层的限域界面胶束组装法显示文摘文章简介课题组首次采用限域界面胶束组装法,在不同材料表面精准地涂覆了一层锐钛矿TiO_2,为有序介孔晶体材料的涂覆打开了一扇新的大门。这些介孔来自于胶束的自组装,而不是纳米晶体的堆积,这种简便、可重复的方法依赖于甘油在组装过程中的限域效应和溶剂选择。整体组装过程有着精确的可控性和极大的通用性,甚至可以调控TiO_2涂层的厚度、介孔孔径等参数。 | Kun Lan Yuan Xia Ruicong Wang Zaiwang Zhao Wei Zhang Xingmiao Zhang Ahmed Elzatahry 赵东元 | 2020 | 科学新闻2020，，2： | 0 |
| 18 | Janus介孔器件用于不同气体的同时检测分析显示文摘文章简介气体传感器在工业和家庭安全、环保及生物医疗等研究领域扮演着不可或缺的角色。迄今为止,传感器件的设计局限于针对单一气体的特定检测,可以同时检测分析不同气体的复合传感器件尚未得到充分探索。复旦大学团队设计制备了一种基于多重响应机制的Janus介孔器件,用于不同气体的同时检测分析。 | Ruicong Wang Kun Lan Zheng Chen Xingmiao Zhang Chin-Te Hung Wei Zhang Changyao Wang Shuai Wang Aibing Chen 李伟 Xin Xu 赵东元 | 2020 | 科学新闻2020，，2： | 0 |
| 19 | CPAL, as a New Mediator of Cardiomyocyte Metabolic Alterations and Pyroptosis, Regulates Myocardial Infarction Injury in Mice显示文摘Myocardial infarction (MI), the most serious of the ischemic heart diseases, is accompanied by myocardial metabolic disorders and the loss of cardiomyocytes. Increasing evidence has shown that long noncoding RNAs (lncRNAs) are involved in various pathological conditions such as cancer and cardiovascular diseases (CVDs), and are emerging as a novel biomarker for these disorders. This study aims to investigate the regulatory role and mechanisms of lncRNAs in myocardial remodeling in the setting of MI. We find that post-infarcted hearts exhibit a reduction of adenosine triphosphate (ATP) and an alteration of the glucose and lipid metabolism genes cluster of differentiation 36 (CD36), hexokinase 1 (HK1), and clucose transporter 4 (GLUT4), accompanied by cardiomyocyte pyroptosis. We then identify a previously unknown conserved lncRNA, AK009126 (cardiomyocyte pyroptosis-associated lncRNA, CPAL), which is remarkably upregulated in the myocardial border zone of MI mice. Importantly, the adeno-associated virus 9 (AAV9)-mediated silencing of endogenous CPAL by its short hairpin RNA (shRNA) partially abrogates myocardial metabolic alterations and cardiomyocyte pyroptosis during MI in mice. Mechanistically, CPAL is shown to bind directly to nuclear factor kappa B (NFκB) and to act as an activator of NFκB to induce NFκB phosphorylation in cardiomyocytes. We also find that CPAL upregulates caspase-1 expression at the transcriptional level and consequently promotes the release of interleukin (IL)-18 and IL-1β from cardiomyocytes. Collectively, our findings reveal the conserved lncRNA CPAL as a new regulator of cardiac metabolic abnormalities and cardiomyocyte pyroptosis in the setting of MI and suggest CPAL as a new therapeutic target to protect cardiomyocytes against ischemic injury in infarcted hearts. | Jiamin Li Hongru Xue Ning Xu Liling Gong Ming Li Sijia Li Di Huang Qingwei Zhang Pengyu Li Qingsui Li Hang Yu Yining Liu Yadong Xue Haixin Chen Jiali Liu Wanyu Zhang Mingbin Liu Siyu Chang Xianzhi Lang Xingmiao Zhao Weijie Du Benzhi Cai Ning Wang Baofeng Yang | 2023 | Engineering2023，，1： | 0 |
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