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| 1 | Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells显示文摘Parthenogenetic embryonic stem cells have pluripotent differentiation potentials,akin to fertilized embryo-derived embryonic stem cells.The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells.Before differentiation,karyotype analysis was performed,with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells.Sex chromosomes were identified as XX.Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene,Oct4,at both the mRNA and protein levels,indicating pluripotent differentiation potential of the two embryonic stem cell subtypes.Embryonic stem cells were induced with retinoic acid to form embryoid bodies,and then dispersed into single cells.Single cells were differentiated in N2 differentiation medium for 9 days.Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin,βIII-tubulin and myelin basic protein.Quantitative real-time PCR found expression of neurogenesis related genes(Sox-1,Nestin,GABA,Pax6,Zic5 and Pitx1) in both types of embryonic stem cells,and Oct4 expression was significantly decreased.Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells.Thus,our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells. | Xingrong Yan Yanhong Yang Wei Liu Wenxin Geng Huichong Du Jihong Cui Xin Xie Jinlian Hua Shumin Yu Liwen Li Fulin Chen | 2013 | Neural Regeneration Research2013，8，4： | 4 |
| 2 | Identification of genes for drought resistance and prediction of gene candidates in soybean seedlings based on linkage and association mapping显示文摘Drought is one of the primary abiotic stress factors affecting the yield,growth,and development of soybeans.In extreme cases,drought can reduce yield by more than 50%.The seedling stage is an important determinant of soybean growth:the number and vigor of seedlings will affect growth and yield at harvest.Therefore,it is important to study the drought resistance of soybean seedlings.In this study,a recombinant inbred line(RIL)population comprising 234 F_(6:10)lines(derived from Zhonghuang35×Jindou 21)and a panel of 259 soybean accessions was subjected to drought conditions to identify the effects on phenotypic traits under these conditions.Using a genetic map constructed by single nucleotide polymorphism(SNPs)markers,18 quantitative trait loci(QTL)on 7 soybean chromosomes were identified in two environments.This included 9 QTL clusters identified in the RIL population.Fifty-three QTL were identified in 19 soybean chromosomes by genome-wide association analysis(GWAS)in the panel of accessions,including 69 significant SNPs(-log_(10)(P)≥3.97).A combination of the two populations revealed that two SNPs(-log_(10)(P)≥3.0)fell within two of the QTL(qPH7-4 and qPH7-6)confidence intervals.We not only re-located several previously reported drought-resistance genes in soybean and other crops but also identified several non-synonymous stress-related mutation site differences between the two parents,involving Glyma.07 g093000,Glyma.07 g093200,Glyma.07 g094100 and Glyma.07 g094200.One previously unreported new gene related to drought stress,Glyma.07 g094200,was found by regional association analysis.The significant SNP CHR7-17619(G/T)was within an exon of the Glyma.07 g094200 gene.In the RIL population,the DSP value of the'T'allele of CHR7-17619 was significantly(P<0.05)larger than the'G'allele in different environments.The results of our study lay the groundwork for cloning and molecular marker-assisted selection of droughtresistance genes in soybeans at the seedling stage. | Yanjun Zhang Zhangxiong Liu Xingrong Wang Yue Li Yongsheng Li Zuowang Gou Xingzhen Zhao Huilong Hong Honglei Ren Xusheng Qi Lijuan Qiu | 2022 | The Crop Journal2022，10，3： | 3 |
| 3 | Serum from Acute-on-chronic Liver Failure Patients May Affect Mesenchymal Stem Cells Transplantation by Impairing the Immunosuppressive Function of Cells显示文摘Background and Aims:The safety and efficacy of mesenchymal stem cells(MSCs)in the treatment of acute-onchronic liver failure(ACLF)have been validated.However,the impact of the pathological ACLF microenvironment on MSCs is less well understood.This study was designed to explore the changes in the functional properties of MSCs exposed to ACLF serum.Methods:MSCs were cultured in the presence of 10%,30%and 50%serum concentrations from ACLF patients and healthy volunteers.Then,the cell morphology,phenotype,apoptosis and proliferation of MSCs were evaluated,including the immunosuppressive effects.Subsequently,mRNA sequencing analysis was used to identify the molecules and pathways involved in MSC functional changes in the context of ACLF.Results:In the presence of ACLF serum,MSC morphology significantly changed but phenotype did not.Besides,MSC proliferation activity was weakened,while the apoptosis rate was lightly increased.Most importantly,the immunosuppressive function of MSCs was enhanced in a lowconcentration serum environment but transformed into a proinflammatory response in a high-concentration serum environment.RNA sequencing indicated that 10%serum concentration from ACLF patients mediated the PI3K-Akt pathway to enhance the anti-inflammatory effect of MSCs,while the 50%serum concentration from ACLF patients promoted the conversion of MSCs into a proinflammatory function by affecting the cell cycle.Conclusions:The 50%ACLF serum concentration is more similar to the environment in the human body,which means that direct peripheral blood intravenous infusion of MSCs may reduce the effect of transplantation.Combining treatments of plasma exchange to reduce harmful substances in serum may promote MSCs to exert a stronger anti-inflammatory effect. | Yongyuan Zheng Shu Zhu Xingrong Zheng Wenxiong Xu Xuejun Li Jianguo Li Zhiliang Gao Chan Xie Liang Peng | 2021 | Journal of Clinical and Translational Hepatology2021，9，4： | 2 |
| 4 | Will the Historic Southeasterly Wind over the Equatorial Pacific in March 2022 Trigger a Third-year La Niña Event?显示文摘Based on the updates of the Climate Prediction Center and International Research Institute for Climate and Society(CPC/IRI)and the China Multi-Model Ensemble(CMME)El Niño-Southern Oscillation(ENSO)Outlook issued in April 2022,La Niña is favored to continue through the boreal summer and fall,indicating a high possibility of a three-year La Niña(2020-23).It would be the first three-year La Niña since the 1998-2001 event,which is the only observed three-year La Niña event since 1980.By examining the status of air-sea fields over the tropical Pacific in March 2022,it can be seen that while the thermocline depths were near average,the southeasterly wind stress was at its strongest since 1980.Here,based on a quaternary linear regression model that includes various relevant air-sea variables over the equatorial Pacific in March,we argue that the historic southeasterly winds over the equatorial Pacific are favorable for the emergence of the third-year La Niña,and both the anomalous easterly and southerly wind stress components are important and contribute~50%of the third-year La Niña growth,respectively.Additionally,the possible global climate impacts of this event are discussed. | Xianghui FANG Fei ZHENG Kexin LI Zeng-Zhen HU Hongli REN Jie WU Xingrong CHEN Weiren LAN Yuan YUAN Licheng FENG Qifa CAI Jiang ZHU | 2023 | Advances in Atmospheric Sciences2023，40，1： | 2 |
| 5 | Another Record: Ocean Warming Continues through 2021 despite La Nina Conditions显示文摘The increased concentration of greenhouse gases in the atmosphere from human activities traps heat within the climate system and increases ocean heat content(OHC). Here, we provide the first analysis of recent OHC changes through 2021 from two international groups. The world ocean, in 2021, was the hottest ever recorded by humans, and the 2021 annual OHC value is even higher than last year’s record value by 14 ± 11 ZJ(1 zetta J = 1021 J) using the IAP/CAS dataset and by16 ± 10 ZJ using NCEI/NOAA dataset. The long-term ocean warming is larger in the Atlantic and Southern Oceans than in other regions and is mainly attributed, via climate model simulations, to an increase in anthropogenic greenhouse gas concentrations. The year-to-year variation of OHC is primarily tied to the El Nino-Southern Oscillation(ENSO). In the seven maritime domains of the Indian, Tropical Atlantic, North Atlantic, Northwest Pacific, North Pacific, Southern oceans,and the Mediterranean Sea, robust warming is observed but with distinct inter-annual to decadal variability. Four out of seven domains showed record-high heat content in 2021. The anomalous global and regional ocean warming established in this study should be incorporated into climate risk assessments, adaptation, and mitigation. | Lijing CHENG John ABRAHAM Kevin ETRENBERTH John FASULLO Tim BOYER Michael EMANN Jiang ZHU Fan WANG Ricardo LOCARNINI Yuanlong LI Bin ZHANG Zhetao TAN Fujiang YU Liying WAN Xingrong CHEN Xiangzhou SONG Yulong LIU Franco RESEGHETTI Simona SIMONCELLI Viktor GOURETSKI Gengxin CHEN Alexey MISHONOV Jim REAGAN | 2022 | Advances in Atmospheric Sciences2022，39，3： | 1 |
| 6 | Evaluation of the intracellular lipid-lowering effect of polyphenols extract from highland barley in HepG2 cells显示文摘Active ingredients from highland barley have received considerable attention as natural products for developing treatments and dietary supplements against obesity.In practical application,the research of food combinations is more significant than a specific food component.This study investigated the lipid-lowering effect of highland barley polyphenols via lipase assay in vitro and HepG2 cells induced by oleic acid(OA).Five indexes,triglyceride(TG),total cholesterol(T-CHO),low density lipoprotein-cholesterol(LDL-C),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),were used to evaluate the lipidlowering effect of highland barley extract.We also preliminary studied the lipid-lowering mechanism by Realtime fluorescent quantitative polymerase chain reaction(q PCR).The results indicated that highland barley extract contains many components with lipid-lowering effects,such as hyperoside and scoparone.In vitro,the lipase assay showed an 18.4%lipase inhibition rate when the additive contents of highland barley extract were 100μg/m L.The intracellular lipid-lowering effect of highland barley extract was examined using 0.25 mmol/L OA-induced HepG2 cells.The results showed that intracellular TG,LDL-C,and T-CHO content decreased by 34.4%,51.2%,and 18.4%,respectively.ALT and AST decreased by 51.6%and 20.7%compared with the untreated hyperlipidemic HepG2 cells.q PCR results showed that highland barley polyphenols could up-regulation the expression of lipid metabolism-related genes such as PPARγand Fabp4. | Yijun Yao Zhifang Li Bowen Qin Xingrong Ju Lifeng Wang | 2024 | Food Science and Human Wellness2024，13，1： | 1 |
| 7 | Preparation and characterization of UV-curable hyperbranched polyurethane acrylate显示文摘 | Gao Qiongzhi Li Hongqiang Zeng Xingrong | 2011 | Journal of Coating Technology & Research2011，8，1： | 1 |
| 8 | Synthesis, photopolymerization kinetics, and thermal properties of UV - curable waterborne hyperbranched poly-urethane acrylate dispersions 显示文摘 | WENHUA YIN XINGRONG ZENG HONi QIANC LI | 2011 | Coat Technol Res2011，8，5： | 1 |
| 9 | Fabrica tion and characterization of stable superhydrophobic fluor inated-polyacrylate/siliea hybrid eoating显示文摘 | Kunquan Li Xingrong Zeng Hongqiang Li | 2014 | Applied Sur face Science2014，298，： | 1 |
| 10 | Steady rheological behaviors of UV-curable waterborne hyperbranched polyurethane acrylate dispersions显示文摘 | Yin Wenhua Zeng Xingrong Li Hongqiang | 2013 | J Coat Technol Res2013，10，1： | 1 |
| 11 | Preparation and Properties of Flame Retardant Polypropylene with an Intumescent System Encapsulated by Thermoplastic Polyurethane显示文摘 | Xuejun Lai Xingrong Zeng Hongqiang Li Feng Liao Haili Zhang Changyu Yin | 2012 | Journal of Macromolecular Science Part B2012，，1： | 1 |
| 12 | Synergistic effect of phosphorus-containing montmorillonite with intu mescent flame retardant in polypropylene 显示文摘 | Lai Xuejun Zeng Xingrong Li Hongqiao | 2012 | Journal of Macromolecular Science: Physics2012，51，6： | 1 |
| 13 | Preparationand characterization of nano-Si O2/ fluorinated polyacrylatecomposite latex via nano-Si O2/ acrylate dispersion显示文摘 | Zhao Fuchun Zeng Xingrong Li Hongqiang | 2010 | Colloidsand Surfaces A2010，353，： | 1 |
| 14 | Preparation andproperties of flame retardant polypropylene with an intumescentsystem encapsulated by thermoplastic polyurethane显示文摘 | Lai Xuejun Zeng Xingrong Li Hongqiang | 2012 | Journal ofMacromolecular Science2012，51，： | 1 |
| 15 | Preparation and characterization of UV-curable hyperbranched polyu- rethane acrylate显示文摘 | Gao Qiongzhi Li Hongqiang Zeng Xingrong | 2011 | J Coat Technol Res2011，8，1： | 1 |
| 16 | Change of retinal nerve fiber layer thickness in patients with nonarteritic inflammatory anterior ischemic optic neuropathy显示文摘In this study, 16 patients (19 eyes) with nonarteritic anterior ischemic optic neuropathy in the acute stage (within 4 weeks) and resolving stage (after 12 weeks) were diagnosed by a series of complete ophthalmic examinations, including fundus examination, optical coherence tomography and fluorescein fundus angiography, and visual field defects were measured with standard automated perimetry. The contralateral uninvolved eyes were used as controls. The retinal nerve fiber layer thickness was determined by optical coherence tomography which showed that the mean retinal nerve fiber layer thickness and the retinal nerve fiber layer thickness from temporal, superior, nasal and inferior quadrants were significantly higher for all measurements in the acute stage than the corresponding normal values. In comparison, the retinal nerve fiber layer thickness from each optic disc quadrant was found to be significantly lower when measured at the resolving stages, than in the control group. Statistical analysis on the correlation between optic disc nerve fiber layer thickness and visual defects demonstrated a positive correlation in the acute stage and a negative correlation in the resolving stage. Our experimental findings indicate that optical coherence tomography is a useful diagnostic method for nonarteritic anterior ischemic optic neuropathy and can be used to evaluate the effect of treatment. | Tingting Liu Hongsheng Bi Xingrong Wang Guimin Wang Haiyan Li Hui Wu Yi Qu Ying Wen Chenyang Cong Daoguang Wang | 2012 | Neural Regeneration Research2012，7，35： | 1 |
| 17 | Photo- polymerization kinetics, and thermal properties of UV-cur- able waterborne hyperbranched polyurethane acrylatedis- persions carboxyl groups显示文摘 | Yin Wenhua Zeng Xingrong Li Hongqiang | 2011 | J Coat Technol Res2011，8，5： | 1 |
| 18 | Preparation and characterization of UV-eurable hyperbranehed polyurethane acrylate显示文摘 | Gao Qiongzhi Li Hongqiang Zeng Xingrong | 2011 | J Coat Teehnol Res2011，8，1： | 1 |
| 19 | Syn- thesis, photopoly-merization kinetics, and thermal prop- erties of UV-curable waterborne byperb-ranched polyu- rethane acrylate dispersions 显示文摘 | Wenhua Yin Xingrong Zeng Hongqiang Li | 2011 | Journal of Coatings Technology and Research2011，8，5： | 1 |
| 20 | Synergistic effectbetween a triazine-based macromolecule and melamine pyrophosphatein flame retardant polypropylene 显示文摘 | Lai Xuejun Zeng Xingrong Li Hongqiang | 2012 | Polymer Composites2012，33，1： | 1 |
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