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| 1 | Effects of Coal Rank and High Organic Sulfur on the Structure and Optical Properties of Coal-based Graphene Quantum Dots显示文摘Coal-based graphene quantum dots(GQDs) were successfully produced via a one-step chemical synthesis from six different coal ranks, from which two superhigh organic sulfur(SHOS) coals were selected as natural S-doped carbon sources for the preparation of S-doped GQDs. The effects of coal properties on coal-based GQDs were analyzed by means of high-resolution transmission electron microscopy(HRTEM), X-ray diffraction(XRD), Fourier transform infrared(FTIR) spectroscopy, X-ray photoelectron spectroscopy(XPS), ultraviolet-visible(UV-Vis) absorption spectroscopy, and fluorescence emission spectra. It was shown that all coal samples can be used to prepare GQDs, which emit bluegreen and blue fluorescence under ultraviolet light. Anthracite-based GQDs have a hexagonal crystal structure without defects, the largest size, and densely arranged carbon rings in their lamellae; the highrank bituminous coal-based GQDs are relatively reduced in size, with their hexagonal crystal structure being only faintly visible; the low-rank bituminous coal-based GQDs are the smallest, with sparse lattice fringes and visible internal defects. As the metamorphism of raw coals increases, the yield decreases and the fluorescence quantum yield(QY) initially increases and then decreases. Additionally, the surface of GQDs that were prepared using high-rank SHOS coal(high-rank bituminous coal) preserves rich sulfur content even after strong oxidation, which effectively adjusts the bandgap and improves the fluorescence QY. Thus, high-rank bituminous coal with SHOS content can be used as a natural S-doped carbon source to prepare S-doped GQDs, extending the clean utilization of low-grade coal. | TANG Yuegang HUAN Xuan LAN Chunyuan XU Miaoxin | 2018 | Acta Geologica Sinica(English Edition)2018，92，3： | 10 |
| 2 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 3 | Surface enhanced Raman spectroscopy signals of mixed pesticides and their identification显示文摘A rapid and sensitive method to identify and analyze mixed pesticides of tricyclazole,paraquat and flusilazole by surface-enhanced Raman scattering has been reported.Aqueous samples can be detected by SERS in low concentrations of 0.01 mg/L, 0.1 mg/L and 2.85 mg/L for individual tricyclazole,paraquat and flusilazole respectively.When mixing the three pesticides in the low concentrations,their characteristic peaks can still be identified from the SERS spectrum of the mixture. | Hui Rong Tang Qing Qing Li Yu Lan Ren Jin Pei Geng Peng Cao Tao Sui Xuan Wang Yi Ping Du | 2011 | Chinese Chemical Letters2011，22，12： | 7 |
| 4 | Sequence framework of two different kinds of margins and their response to tectonic activity during the Middle-Late Triassic, Ordos Basin显示文摘Two kinds of margin respectively occur in the Ordos Basin during the Middle-Late Triassic (Yanchang Age), one is foreland margin developed under the background of flexural subsidence by thrusting intensively in the southwest margin, and the other is intracratonic basin margin by stable subsidence in northern and central parts of the basin. The Middle-Late Triassic Yanchang Formation can be divided into four regional third-order sequences, which are separated by gentle angular unconformity or regional erosion surface, made up of lowstand system tract (LST), expanding system tract (EST) and highstand system tract (HST) from lower to upper within a sequence. But there are distinct differences of the sequence framework between the southwest margin and northern and central parts of the basin. The southwest margin develops heavy conglomerate layer and unconformity as a result of orogeny by thrusting, and the intracratonic basin margin by stable subsidence in the northern and central parts grows aggradational sandstone, conglomerate in fluvio-delta system and parallel unconformity. The depositional framework of southwest margin reflects the tectonic evolution from flexural subsidence by thrusting to rebounded uplift. The formation of sequence boundary is related to the resilient uplift and erosion. The sequence stratigraphic framework and depositional system tract configuration in the foreland basin are controlled by structural activity of the fold and thrust belt, and the sequence succession reflects episodic thrusting of the Middle-Late Triassic toward the foreland basin. The sequence evolution in northern and central parts reflects the depositional succession of fluvio-delta system under intracratonic background, composed of coarse-grained sediment in braided channel deposit at the lower, meandering channel deposit in the middle and fine-grained sediment in the flood plain at the upper, dominated by lake level fluctuation. During the deposit of the LST in the intracraton basin, accommodation space is limited, and results in abundant fluvial sediment migration laterally, erosion and transport, forming laterally sandstone composite and aggradational deposit on the alluvial plain, which constitutes specific erosion unconformity boundary. | YANG MingHui LIU ChiYang ZHENG MengLin LAN ChaoLi TANG Xuan | 2007 | Science China Earth Sciences2007，50，z2： | 7 |
| 5 | Molecular mechanisms of dietary restriction in aging—insights from Caenorhabditis elegans research显示文摘Dietary restriction(DR) is one of the most robust environmental manipulations that not only extend life span but also delay the onset of age-related diseases in almost every species examined. Caenorhabditis elegans plays an important role in aging studies due to its simple life cycle, easy genetic manipulations and highly conserved genome. Recent studies have demonstrated that the beneficial effects of DR are mediated by the highly conserved transcription factors and signaling pathways in C. elegans. Here we review recent progress in the methodology and molecular mechanisms of DR using C. elegans as a model, as well as prospects for future research. | LAN JianFeng ZHANG Xuan CHEN Di | 2015 | Science China(Life Sciences)2015，58，4： | 6 |
| 6 | Pharmacokinetics-based identification of pseudoaldosterogenic compounds originating from Glycyrrhiza uralensis roots(Gancao)after dosing LianhuaQingwen capsule显示文摘LianhuaQingwen capsule,prepared from an herbal combination,is officially recommended as treatment for COVID-19 in China.Of the serial pharmacokinetic investigations we designed to facilitate identifying LianhuaQingwen compounds that are likely to be therapeutically important,the current investigation focused on the component Glycyrrhiza uralensis roots(Gancao).Besides its function in COVID-19 treatment,Gancao is able to induce pseudoaldosteronism by inhibiting renal 11β-HSD2.Systemic and colon-luminal exposure to Gancao compounds were characterized in volunteers receiving LianhuaQingwen and by in vitro metabolism studies.Access of Gancao compounds to 11β-HSD2 was characterized using human/rat,in vitro transport,and plasma protein binding studies,while 11β-HSD2 inhibition was assessed using human kidney microsomes.LianhuaQingwen contained a total of 41 Gancao constituents(0.01-8.56μmol/day).Although glycyrrhizin(1),licorice saponin G2(2),and liquiritin/liquiritin apioside(21/22)were the major Gancao constituents in LianhuaQingwen,their poor intestinal absorption and access to colonic microbiota resulted in significant levels of their respective deglycosylated metabolites glycyrrhetic acid(8),24-hydroxyglycyrrhetic acid(M2_(D);a new Gancao metabolite),and liquiritigenin(27)in human plasma and feces after dosing.These circulating metabolites were glucuronized/sulfated in the liver and then excreted into bile.Hepatic oxidation of 8 also yielded M2_(D).Circulating 8 and M2D,having good membrane permeability,could access(via passive tubular reabsorption)and inhibit renal 11β-HSD2.Collectively,1 and 2 were metabolically activated to the pseudoaldosterogenic compounds 8 and M2_(D).This investigation,together with such investigations of other components,has implications for precisely defining therapeutic benefit of LianhuaQingwen and conditions for its safe use. | Xiao-fang Lan Olajide EOlaleye Jun-lan Lu Wei Yang Fei-fei Du Jun-ling Yang Chen Cheng Yan-hong Shi Feng-qing Wang Xue-shan Zeng Nan-nan Tian Pei-wei Liao Xuan Yu Fang Xu Ying-fei Li Hong-tao Wang Nai-xia Zhang Wei-wei Jia Chuan Li | 2021 | Acta Pharmacologica Sinica2021，42，12： | 6 |
| 7 | Association between lumican gene-1554 T/C polymorphism and high myopia in Asian population:a meta-analysis显示文摘AIM:To investigate the association between lumican gene-1554 T/C polymorphism and high myopia susceptibility.METHODS:We searched the published literature in the Medline,Embase,and CBM databases from inception to July 2013.A meta-analysis was performed by the programs RevMan 5.1 and Stata 12.0,and the odds ratio(OR) with 95% confidence interval(CI) was calculated in fixed or random effect model based on heterogeneity test among studies.RESLUTS:Seven case-control studies with a total of 1 233 cases and 936 controls were included.A statistical significant association with high myopia was observed in the recessive model(TT vs CT +CC:OR=1.92;95% CI=1.14-3.23) and codominant model(TT vs CT:OR=1.81,95%CI=1.19-2.75).CONCLUSION:The present meta-analysis suggested that lumican-1554 T/C polymorphism might be moderately associated with high myopia susceptibility.This conclusion warrants confirmation by further studies. | Xuan Liao Xu-Bo Yang Meng Liao Chang-Jun Lan Long-Qian Liu | 2013 | International Journal of Ophthalmology(English edition)2013，6，5： | 5 |
| 8 | Hydrocarbon Systems in the North-Eastern Area of the Song Hong Basin显示文摘 | Trinh Xuan Cuong Phan Ngoc Trung Tong Duy Cuong Nguyen Bich Ha Phung Lan Phtro'ng Hoang ThiLan Nguyen Hoang Son | 2013 | Journal of Earth Science and Engineering2013，3，12： | 3 |
| 9 | Study on the pathogenetic effect of salted pork from a high risk area of stomach cancer in China显示文摘StudyonthepathogeneticefectofsaltedporkfromahighriskareaofstomachcancerinChinaYUANYuan1,LINHuiZhi1,ZHANGYinChang1,WANGXuan... | YUAN Yuan 1, LIN Hui Zhi 1, ZHANG Yin Chang 1, WANG Xuan Jie 2, WU Yie Qiu 1, GAO Hua 1, WANG Lan 1, LIU Yan Hou 3, LU Fang 3 and LOU Su Qing 3 | 1997 | World Journal of Gastroenterology1997，3，2： | 3 |
| 10 | Disruption of the bHLH transcription factor Abnormal Tapetum 1 causes male sterility in watermelon显示文摘Although male sterility has been identified as a useful trait for hybrid vigor utilization and hybrid seed production,its underlying molecular mechanisms in Cucurbitaceae species are still largely unclear.Here,a spontaneous male-sterile watermelon mutant,Se18,was reported to have abnormal tapetum development,which resulted in completely aborted pollen grains.Map-based cloning demonstrated that the causal gene Citrullus lanatus Abnormal Tapetum 1(ClATM1)encodes a basic helix-loop-helix(bHLH)transcription factor with a 10-bp deletion and produces a truncated protein without the bHLH interaction and functional(BIF)domain in Se18 plants.qRT–PCR and RNA in situ hybridization showed that ClATM1 is specifically expressed in the tapetum layer and in microsporocytes during stages 6–8a of anther development.The genetic function of ClATM1 in regulating anther development was verified by CRISPR/Cas9-mediated mutagenesis.Moreover,ClATM1 was significantly downregulated in the Se18 mutant,displaying a clear dose effect at the transcriptional level.Subsequent dual-luciferase reporter,β-glucuronidase(GUS)activity,and yeast one-hybrid assays indicated that ClATM1 could activate its own transcriptional expression through promoter binding.Collectively,ClATM1 is the first male sterility gene cloned from watermelon,and its self-regulatory activity provides new insights into the molecular mechanism underlying anther development in plants. | Ruimin Zhang Jingjing Chang Jiayue Li Guangpu Lan Changqing Xuan Hao Li Jianxiang Ma Yong Zhang Jianqiang Yang Shujuan Tian Li Yuan Xian Zhang Chunhua Wei | 2021 | Horticulture Research2021，8，1： | 3 |
| 11 | Polyguluronate Sulfate and Its Oligosaccharides but not Heparin Promotes FGF19/FGFR1c Signaling显示文摘Fibroblast growth factor 19(FGF19) functions as a hormone by affecting glucose metabolism. FGF19 improves glucose tolerance when overexpressed in mice with impaired glucose tolerance or diabetes. A functional cellular FGF19 receptor consists of FGF receptor(FGFR) and glycosaminoglycan complexed with either α Klotho or β Klotho. Interestingly, in mice with diet-induced diabetes, a single injection of FGF1 is enough to restore blood sugar levels to a healthy range. FGF1 binds heparin with high affinity whereas FGF19 does not, indicating that polysaccharides other than heparin might enhance FGF19/FGFR signaling. Using a FGFs/FGFR1 c signaling-dependent Ba F3 cell proliferation assay, we discovered that polyguluronate sulfate(PGS) and its oligosaccharides, PGS12 and PGS25, but not polyguluronate(PG), a natural marine polysaccharide, enhanced FGF19/FGFR1 c signaling better than that of heparin based on ~3H-thymidine incorporation. Interestingly, PGS6, PGS8, PGS10, PGS12, PGS25, and PGS, but not PG, had comparable FGF1/FGFR1 c signal-stimulating activity compared to that of heparin. These results indicated that PGS and its oligosaccharides were excellent FGF1/FGFR1 c and FGF19/FGFR1 c signaling enhancers at cellular level. Since the inexpensive PGS and PGS oligosaccharides can be absorbed through oral route, these seaweed-derived compounds merit further investigation as novel agents for the treatment of type 2 diabetes through enhancing FGF1/FGFR1 c and FGF19/FGFR1 c signaling in future. | LAN Ying ZENG Xuan GUO Zhihua ZENG Pengjiao HAO Cui ZHAO Xia YU Guangli ZHANG Lijuan | 2017 | Journal of Ocean University of China2017，16，3： | 2 |
| 12 | Myopia genetics in genome-wide association and postgenome-wide association study era显示文摘Genome-wide association studies(GWAS) of myopia and refractive error have generated exciting results and identified novel risk-associated loci. However, the interpretation of the findings of GWAS of complex diseases is not straightforward and has remained challenging. This review provides a brief summary of the main focus on the advantages and limitations of GWAS of myopia, with potential strategies that may contribute to further insight into the genetics of myopia in the post-GWAS or omics era. | Xuan Liao Qing-Qing Tan Chang-Jun Lan | 2019 | International Journal of Ophthalmology(English edition)2019，12，9： | 2 |
| 13 | Melatonin ameliorates bleomycin-induced pulmonary fibrosis via activating NRF2 and inhibiting galectin-3 expression显示文摘Pulmonary fibrosis(PF)is a chronic interstitial lung disease with no effective therapies.Galectin-3(Gal-3),a marker of oxidative stress,plays a key role in the pathogenesis of PF.Fibroblast-myofibroblast differentiation(FMD)is an important source of fibrotic cells in PF.Previous studies showed that melatonin(MT)exerted anti-fibrotic effect in many diseases including PF through its antioxidant activity.In the present study we investigated the relationships among Gal-3,NRF2,ROS in FMD and their regulation by MT.We established an in vitro model of FMD in TGF-β1-treated human fetal lung fibroblast1(HFL1)cells and a PF mouse model via bleomycin(BLM)intratracheal instillation.We found that Gal-3 expression was significantly increased both in vitro and in vivo.Knockdown of Gal-3 in HFL1 cells markedly attenuated TGF-β1-induced FMD process and ROS accumulation.In TGF-β1-treated HFL1 cells,pretreatment with NRF2-specific inhibitor ML385(5μM)significantly increased the levels of Gal-3,α-SMA and ROS,suggesting that the expression of Gal-3 was regulated by NRF2.Treatment with NRF2-activator MT(250μM)blockedα-SMA and ROS accumulation accompanied by reduced Gal-3 expression.In BLM-induced PF model,administration of MT(5 mg·kg^(−1)·d^(−1),ip for 14 or 28 days)significantly attenuated the progression of lung fibrosis through up-regulating NRF2 and down-regulating Gal-3 expression in lung tissues.These results suggest that Gal-3 regulates TGF-β1-induced pro-fibrogenic responses and ROS production in FMD,and MT activates NRF2 to block FMD process by down-regulating Gal-3 expression.This study provides a useful clue for a clinical strategy to prevent PF. | Yue-jiao Lan Ming-han Cheng Hui-min Ji Yu-qian Bi Yong-yue Han Chong-yang Yang Xuan Gu Jian Gao Hong-liang Dong | 2023 | Acta Pharmacologica Sinica2023，44，5： | 2 |
| 14 | Comparison of visual quality after implantation of Al-UV and SN60WF aspheric intraocular lens显示文摘AIM:To compare the visual performance of pseudophakic eyes implanted with Al-UV and SN60WF aspheric intraocular lens(IOL),and to investigate the correlations between visual quality parameters and pupil size.METHODS:This prospective comparative study included 105 eyes of 90 patients with age-related cataract who underwent uneventful phacoemulsification.The subjects were divided into two groups according to the implanted IOL type.Three months postoperatively,visual acuity and contrast sensitivity were measured,wave-front aberrations were assessed using a KR-IW aberrometer(Topcon),and objective optical quality parameters were performed using an optical quality analysis system-OQAS Il(Visiometrics).Independent sample Mest and Spearman correlation analysis were used for data analysis.RESULTS:There were no significant differences found in visual acuity,contrast sensitivity and visual quality parameters between the two groups(P>0.05).The measured intraocular spherical aberration(SA)in Al-UV IOL eyes of-0.19±0.05 pm was close to the designed SA value of-0.20 pm.The modulation transfer function cutoff,Strehl ratio and OQAS values were negatively correlated with pupil size in both groups(P<0.01).CONCLUSION:The subjective and objective visual quality in pseudophakic eyes with A1-UV and SN60WF IOLs are comparable.For aspheric IOL eyes,visual quality decreases with increasing pupil size. | Xuan Liao Ji-Yun Li Qing-Qing Tan Jing Tian Jia Lin Chang-Jun Lan | 2020 | International Journal of Ophthalmology(English edition)2020，13，11： | 2 |
| 15 | Risk stratification in symptomatic intracranial atherosclerotic disease with conventional vascular risk factors and cerebral haemodynamics显示文摘Background and purpose Symptomatic intracranial atherosclerotic stenosis(sICAS)is associated with a considerable risk of recurrent stroke despite contemporarily optimal medical treatment.Severity of luminal stenosis in sICAS and its haemodynamic significance quantified with computational fluid dynamics(CFD)models were associated with the risk of stroke recurrence.We aimed to develop and compare stroke risk prediction nomograms in sICAS,based on vascular risk factors and these metrics.Methods Patients with 50%-99%sICAS confirmed in CT angiography(CTA)were enrolled.Conventional vascular risk factors were collected.Severity of luminal stenosis in sICAS was dichotomised as moderate(50%-69%)and severe(70%-99%).Translesional pressure ratio(PR)and wall shear stress ratio(WSSR)were quantified via CTA-based CFD modelling;the haemodynamic status of sICAS was classified as normal(normal PR&WSSR),intermediate(otherwise)and abnormal(abnormal PR&WSSR).All patients received guideline-recommended medical treatment.We developed and compared performance of nomograms composed of these variables and independent predictors identified in multivariate logistic regression,in predicting the primary outcome,recurrent ischaemic stroke in the same territory(SIT)within 1 year.Results Among 245 sICAS patients,20(8.2%)had SIT.The D2H2A nomogram,incorporating diabetes,dyslipidaemia,haemodynamic status of sICAS,hypertension and age≥50 years,showed good calibration(P for Hosmer-Lemeshow test=0.560)and discrimination(C-statistic 0.73,95%CI 0.60 to 0.85).It also had better performance in risk reclassification and provided larger net benefits in decision curve analysis,compared with nomograms composed of conventional vascular risk factors only,and plus the severity of luminal stenosis in sICAS.Sensitivity analysis in patients with anterior-circulation sICAS showed similar results.Conclusions The D2H2A nomogram,incorporating conventional vascular risk factors and the haemodynamic significance of sICAS as assessed in CFD models,could be a useful tool to stratify sICAS patients for the risk of recurrent stroke under contemporarily optimal medical treatment. | Xuan Tian Hui Fang Linfang Lan Hing Lung Ip Jill Abrigo Haipeng Liu Lina Zheng Florence S Y Fan Sze Ho Ma Bonaventure Ip Bo Song Yuming Xu Jingwei Li Bing Zhang Yun Xu Yannie O Y Soo Vincent Mok Ka Sing Wong Thomas W Leung Xinyi Leng | 2023 | Stroke & Vascular Neurology2023，8，1： | 2 |
| 16 | Molecular Epidemiology and Risk Factors of Carbapenem-Resistant Klebsiella Pneumoniae Bloodstream Infections in Wuhan,China显示文摘Objective:The clinical characteristics and microbiological data of patients with K.pneumoniae bloodstream infections(BSI)from January 2018 to December 2020 were retrospectively analyzed to study the molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae(CRKP).We also aimed to identify the risk factors for the development of CRKP BSI.Methods:This retrospective study was conducted at Renmin Hospital of Wuhan University from January 2018 to December 2020.The date of non-duplicate K.pneumoniae isolates isolated from blood samples was identified using the microbiology laboratory database.The data from patients diagnosed with K.pneumoniae BSI were collected and analyzed. | Chan LIU Lan LIU Ming-ming JIN Yang-bo HU Xuan CAI Lu WAN Hai-yue ZHANG Rui-yun LI Xiao-jun WU | 2022 | Current Medical Science2022，42，1： | 1 |
| 17 | Heck reaction catalyzed by Pd supported on LDH - F hydrotalcite 显示文摘 | Zhou Hao Zhuo Guang Lan Jiang Xuan Zhen | 2006 | Journal of Molecular Catalysis A : Chemical2006，248，： | 1 |
| 18 | Interracial microstructure and characterization of double-layer coatings on cast iron by arc spraying and sintering显示文摘 | LAN Dong-yun WANG Qiang XUAN Zhao-zhi | 2008 | Materials Science and Engineering A2008，473，12： | 1 |
| 19 | All-trans retinoic acid regulates the expression of MMP-2 and TGF-β2 via RDH5 in retinal pigment epithelium cells显示文摘·AIM: To investigate the effect of all-trans retinoic acid(ATRA) on retinol dehydrogenase 5(RDH5), matrix metalloproteinase-2(MMP-2) and transforming growth factor-β2(TGF-β2) transcription levels, and the effect of RDH5 on MMP-2 and TGF-β2 in retinal pigment epithelium(RPE) cells.·METHODS: After adult RPE cell line-19(ARPE-19 cells) intervened with gradient concentrations of ATRA(0-20 μmol/L) for 24h, flow cytometry was used to detect the proliferation and apoptosis of cells in each group, and quantitative realtime polymerase chain reaction(q RT-PCR) was used to detect RDH5, MMP-2 and TGF-β2 m RNA expression. Then, after ARPE-19 cells transfected with three different si RNA targets for 48h, the RDH5 knockdown efficiency of each group and expression of MMP-2 and TGF-β2 m RNA within them was detected by q RT-PCR. ·RESULTS: Flow cytometry results showed that ATRA could inhibit the proliferation of RPE cells and promote the apoptosis of RPE cells, and the difference of apoptosis was statistically significant when the ATRA concentration exceeded 5 μmol/L and compared with the normal control group(P=0.027 and P=0.031, respectively). q RT-PCR results showed that ATRA could significantly inhibit the expression level of RDH5 m RNA(P<0.001) and promote the expression of MMP-2 and TGF-β2 m RNA(P=0.03 and P<0.001, respectively) in a dose-dependent manner, especially when treated with 5 μmol/L ATRA. The knockdown efficiency of RDH5 si RNA varies with different targets, among which RDH5 si RNA-435 had the highest knockdown efficiency, i.e., more than 50% lower than that of the negative control group(P=0.02). When RDH5 was knocked down for 48h, the results of q RT-PCR showed that the expressions of MMP-2 and TGF-β2 m RNA were significantly up-regulated(P<0.001).·CONCLUSION: ATRA inhibits the expression of RDH5 and promotes MMP-2 and TGF-β2, and further RDH5 knockdown significantly upregulates MMP-2 and TGF-β2. These findings suggest that RDH5 may be involved in an epithelial-mesenchymal transition of RPE cells mediated by ATRA. | Yu-Mei Mao Chang-Jun Lan Qing-Qing Tan Gui-Mei Zhou Xiao-Ling Xiang Jia Lin Xuan Liao | 2023 | International Journal of Ophthalmology(English edition)2023，16，6： | 1 |
| 20 | 查看详情显示文摘 | Hu W Zhang T Guo Q Xuan L Lan S | | 0，，： | 1 |
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