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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | 运用气相色谱-嗅觉测量法和气质联用法分析三个桂花品种的香气活性物质(英文)显示文摘研究目的:分析不同桂花品种感官评价差异所对应的香气成分,及其有贡献的香气活性物质,为桂花的生物科学应用提供依据。创新要点:首次运用气相色谱-嗅觉测量法(GC-O)结合气质联用法(GC-MS)对所分离的挥发性物质进行定性和半定量分析,并同时结合其气味描述,分析不同桂花品种的香气活性物质特征。本研究还根据所检测的香气活性物质的气味特征对香气活性物质进行分组,能更直观地分析桂花不同品种香气差异的原因。研究方法:(1)运用GC-MS对三个桂花品种的挥发性物质进行定性和半定量的比较分析(见表1);(2)运用GC-O对三个桂花品种香气活性物质进行比较分析(见表2);(3)对香气活性物质进行分组,探究不同桂花品种的香气差异原因(见表3和图2)。重要结论:(1)GC-O结合GC-MS分析所得香气活性物质并非都是含量较高的挥发性物质,有些含量较低的挥发性物质也对桂花香气形成有贡献;(2)不同桂花品种的香气差异是由不同香气分组以及香气活性物质强度不同所致。 | Xuan CAI Rong-zhang MAI Jing-jing ZOU Hong-yan ZHANG Xiang-ling ZENG Ri-ru ZHENG Cai-yun WANG | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，7： | 12 |
| 3 | Comparative proteogenomic analysis of the Leptospira interrogans virulence-attenuated strain IPAV against the pathogenic strain 56601显示文摘稀释毒力的螺旋体 interrogans serovar Lai 紧张 IPAV 被延长实验室经过从在中国孤立的高度剧毒的祖先的紧张导出。我们学习了 IPAV 的基因变化对病原的 L 经由比较分析使它变为无毒害。interrogans serovar Lai 紧张 56601。IPAV 紧张的完全的染色体顺序决心、使用与作比较,然后修正并且重新注解紧张 56601 的染色体顺序。除了他们的高度类似的 genomic,结构和基因顺序, 33 插入的一个总数, 53 删除和 301 个单个核苷酸的变化(SNV ) 在整个直接影响 101 基因的 IPAV 的染色体被检测,在他们的 5 ′ 的任何一个;在上游的区域或在他们的编码区域以内。在他们之中, 44 功能的基因的多数涉及信号 transduction,压力反应, transmembrane 运输和氮新陈代谢。基于量的液体,层析(LC )-MS/MS 数据揭示了的比较 proteomic 分析在 orthologs 的 1 627 选择的对之中,在 IPAV 的 174 基因拉紧,这是 upregulated,与主要在精力的班上的丰富生产和类脂化合物新陈代谢。相反,在紧张 56601 的 228 基因是 upregulated,与在蛋白质翻译和 DNA 复制 / 修理的范畴充实的多数。 genomic 和 proteomic 途径的联合在批评基因说明了那改变的表情或变化,例如编码 Ser/Thr kinase ,碳饥饿蛋白质 CstA ,夫酸安合成酶,GTP有约束力的蛋白质 BipA , ribonucleotide-diphosphate reductase 和磷酸盐 transporter 的那些,并且在脂蛋白或外部膜蛋白质的翻译侧面的改变是可能的在紧张 IPAV 说明毒力变细。 | Yi Zhong Xiao Chang Xing-Jun Cao Yan Zhang Huajun Zheng Yongzhang Zhu Chengsong Cai Zelin Cui Yunyi Zhang Yuan-Yuan Li Xiu-Gao Jiang Guo-Ping Zhao Shengyue Wang Yixue Li Rong Zeng Xuan Li Xiao-Kui Guo | 2011 | Cell Research2011，21，8： | 10 |
| 4 | Hard X-ray Imager (HXI) onboard the ASO-S mission显示文摘Hard X-ray Imager(HXI)is one of the three scientific instruments onboard the Advanced Spacebased Solar Observatory(ASO-S)mission,which is proposed for the 25th solar maximum by the Chinese solar community.HXI is designed to investigate the non-thermal high-energy electrons accelerated in solar flares by providing images of solar flaring regions in the energy range from 30 keV to 200 keV.The imaging principle of HXI is based on spatially modulated Fourier synthesis and utilizes about 91 sets of bi-grid sub-collimators and corresponding LaBr3 detectors to obtain Fourier components with a spatial resolution of about 3 arcsec and a time resolution better than 0.5 s.An engineering prototype has been developed and tested to verify the feasibility of design.In this paper,we present background,instrument design and the development and test status of the prototype. | Zhe Zhang Deng-Yi Chen Jian Wu Jin Chang Yi-Ming Hu Yang Su Yan Zhang Jian-Ping Wang Yao-Ming Liang Tao Ma Jian-Hua Guo Ming-Sheng Cai Yong-Qiang Zhang Yong-Yi Huang Xiao-Yan Peng Zong-Bin Tang Xuan Zhao Hong-He Zhou Lian-Guo Wang Jing-Xing Song Miao Ma Guang-Zhou Xu Jian-Feng Yang Di Lu Ying-Hong He Jin-You Tao Xiao-Long Ma Bao-Gang Lv Yan-Ping Bai Cai-Xia Cao Yu Huang Wei-Qun Gan | 2019 | Research in Astronomy and Astrophysics2019，19，11： | 9 |
| 5 | Molecular characteristics and structure–activity relationships of food-derived bioactive peptides显示文摘Peptides are functional active fragments of proteins which can provide nutrients needed for human growth and development,and they also have unique physiological activity characteristics relative to proteins.Bioactive peptides contain a great deal of development potential.More specifically,food-derived bioactive peptides have the advantages of a wide variety of sources,unique structures,high efficiency and safety,so they have broad development prospects.This review provides an overview of the current advances regarding the preparation,functional characteristics,and structure–activity relationships of food-derived bioactive peptides.Moreover,the prospects for the future development and application of food-derived bioactive peptides are discussed.This review may provide a better understanding of foodderived bioactive peptides,and some constructive inspirations for further research and applications in the food industry. | YANG Fu-jia CHEN Xu HUANG Mu-chen YANG Qian CAI Xi-xi CHEN Xuan DU Ming HUANG Jian-lian WANG Shao-yun | 2021 | Journal of Integrative Agriculture2021，20，9： | 9 |
| 6 | Visible light-mediated C—P bond formation reactions显示文摘Organophosphorus compounds have attracted continuous attention in materials science, agrochemical and pharmaceutical fields due to their unique bioactivities. Thus, the development of novel and robust manners for the construction new C—P bond has therefore gained great interests in synthetic organic chemistry. Because of their intrinsic sustainability and green chemistry character, visible light-induced photoredox catalysis has been widely applied in the construction of new chemical bonds, including the formation of C—P bond. In this review, we summarized recent achievements in C—P bond formation reactions initiated by visible light-induced photoredox catalysis, which mainly focusing on the discussion of reaction design and the mechanism. | Bao-Gui Cai Jun Xuan Wen-Jing Xiao | 2019 | Science Bulletin2019，64，5： | 8 |
| 7 | Simultaneous determination of seven principal constituents in Asari Radix et Rhizoma by HPLC显示文摘建立了同时测定细辛中花椒酚、卡枯醇、甲基丁香酚、l–芝麻脂素、黄樟醚、细辛素和l–细辛脂素7种主要成分含量的HPLC方法,并测定了55份不同来源的细辛药材(35份北细辛、3份汉城细辛和17份华细辛)中这7种成分的含量,比较了不同品种细辛中7种成分含量差异。采用Agilent Zorbax SB–C18色谱柱(4.6 mm×25 cm,5μm),水(A)–乙腈(B)为流动相进行梯度洗脱55 min,DAD检测器检测,柱温20°C,检测波长287 nm。被测成分在测试范围内线性关系良好,r2>0.999;加样回收率96.0%–103.1%,RSD小于2.54%。该方法准确、灵敏、重复性好。测定结果表明不同来源的细辛样品中7种成分的含量差异显著,其中挥发性成分甲基丁香酚在辽细辛(汉城细辛与北细辛的根及根茎)中的含量为(6.17±5.13)mg/g,显著高于其在华细辛(华细辛的根及根茎)中的含量(0.58±0.39)mg/g,有毒成分黄樟醚在辽细辛中的含量为(2.66±2.16)mg/g,低于其在华细辛的含量(6.44±3.88)mg/g。本文首次报道了细辛中花椒酚和细辛素的含量。 | Chen Cao Jingzhe Wang Lu Wang Mingying Shang Guangxue Liu Feng Xu Xuan Wang Shaoqing Cai | 2015 | Journal of Chinese Pharmaceutical Sciences2015，24，8： | 6 |
| 8 | Analysis of aristolochic acids, aristololactams and their analogues using liquid chromatography tandem mass spectrometry显示文摘More than 80 aristolochic acids(AAs) and aristololactams(ALs) have been found in plants of the Aristolochiaceae family, but relatively few have been fully studied. The present study aimed at developing and validating a liquid chromatography tandem mass spectrometry(LC/MS^n) for the analysis of these compounds. We characterized the fragmentation behaviors of 31 AAs, ALs, and their analogues via high performance liquid chromatography coupled with electrospray ionization mass spectrometry. We summarized their fragmentation rules and used these rules to identify the constituents contained in Aristolochia contorta, Ar. debilis, Ar. manshurensis, Ar. fangchi, Ar. cinnabarina, and Ar. mollissima. The AAs and ALs showed very different MS behaviors. In MS1 of AAs, the characteristic pseudomolecular ions were [M + NH_4]^+, [M + H]^+, and [M + H - H_2O]^+. However, only [M + H]^+ was found in the MS1 of ALs, which was simpler than that of AAs. Distinct MSn fragmentation patterns were found for AAs and ALs, showing the same skeleton among the different substituent groups. The distribution of the 31 constituents in the 6 species of Aristolochia genus was reported for the first time. 25 Analogues of AAs and ALs were detected in this genus. A hierarchical schemes and a calculating formula of the molecular formula of these nitrophenanthrene carboxylic acids and their lactams were proposed. In conclusion, this method could be applied to identification of similar unknown constituents in other plants. | YU Jie MA Chao-Mei WANG Xuan SHANG Ming-Ying Masao Hattori XU Feng JING Yu DONG Shi-Wen XU Yu-Qiong ZHANG Cui-Ying CAI Shao-Qing | 2016 | Chinese Journal of Natural Medicines2016，14，8： | 6 |
| 9 | Screening potential mitochondria-targeting compounds from traditional Chinese medicines using a mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry method显示文摘Mitochondria regulate numerous crucial cell processes, including energy production, apoptotic cell death, oxidative stress, calcium homeostasis and lipid metabolism. Here, we applied an efficient mitochondria-based centrifugal ultrafiltration/liquid chromatography/mass spectrometry(LC/MS) method,also known as screening method for mitochondria-targeted bioactive constituents(SM-MBC). This method allowed searching natural mitochondria-targeting compounds from traditional Chinese medicines(TCMs), including Puerariae Radix(PR) and Chuanxiong Radix(CR). A total of 23 active compounds were successfully discovered from the two TCMs extracts. Among these 23 hit compounds, 17 were identified by LC/MS, 12 of which were novel mitochondria-targeting compounds. Among these, 6 active compounds were analyzed in vitro for pharmacological tests and found able to affect mitochondrial functions. We also investigated the effects of the hit compounds on Hep G2 cell proliferation and on loss of cardiomyocyte viability induced by hypoxia/reoxygenation injury. The results obtained are useful for in-depth understanding of mechanisms underlying TCMs therapeutic effects at mitochondria level and for developing novel potential drugs using TCMs as lead compounds. Finally, we showed that SM-MBC was an efficient protocol for the rapid screening of mitochondria-targeting constituents from complex samples such as PR and CR extracts. | Xing-Xin Yang Yu-Zhen Zhou Feng Xu Jie Yu Gegentana Ming-Ying Shang Xuan Wang Shao-Qing Cai | 2018 | Journal of Pharmaceutical Analysis2018，8，4： | 5 |
| 10 | Whole-genome resequencing of Osmanthus fragrans provides insights into flower color evolution显示文摘Osmanthus fragrans is a well-known ornamental plant that has been domesticated in China for 2500 years.More than 160 cultivars have been found during this long period of domestication,and they have subsequently been divided into four cultivar groups,including the Yingui,Jingui,Dangui,and Sijigui groups.These groups provide a set of materials to study genetic evolution and variability.Here,we constructed a reference genome of O.fragrans‘Liuyejingui’in the Jingui group and investigated its floral color traits and domestication history by resequencing a total of 122 samples,including 119 O.fragrans accessions and three other Osmanthus species,at an average sequencing depth of 15×.The population structure analysis showed that these 119 accessions formed an apparent regional cluster.The results of linkage disequilibrium(LD)decay analysis suggested that varieties with orange/red flower color in the Dangui group had undergone more artificial directional selection;these varieties had the highest LD values among the four groups,followed by the Sijigui,Jingui,and Yingui groups.Through a genome-wide association study,we further identified significant quantitative trait loci and genomic regions containing several genes,such as ethylene-responsive transcription factor 2 and Arabidopsis pseudoresponse regulator 2,that are positively associated with petal color.Moreover,we found a frameshift mutation with a 34-bp deletion in the first coding region of the carotenoid cleavage dioxygenase 4 gene.This frameshift mutation existed in at least one site on both alleles in all varieties of the Dangui group.The results from this study shed light on the genetic basis of domestication in woody plants,such as O.fragrans. | Hongguo Chen Xiangling Zeng Jie Yang Xuan Cai Yumin Shi Riru Zheng Zhenqi Wang Junyi Liu Xinxin Yi Siwei Xiao Qiang Fu Jingjing Zou Caiyun Wang | 2021 | Horticulture Research2021，8，1： | 5 |
| 11 | Porous surfur-doped hard carbon for excellent potassium storage显示文摘Hard carbon is promising anode for potassium-ion batteries(PIBs),however,the poor rate capability hinders its development as potential anode.To address this question,we design a sulfur-doped porous hard carbon(S-HC)for PIBs through the combination of structural design and composition adjustment.The as-designed S-HC exhibits a long cycling life with^191 mAh/g after 300 cycles at 1 A/g,and an excellent rate capability with^100 mAh/g at 5 A/g,which was attributed to its structural characteristics and compositions.The S-HC demonstrates to be promising anode in the future. | Xuan Xie Shihan Qi Daxiong Wu Huaping Wang Fang Li Xiaoxin Peng Jinfeng Cai Jiaojiao Liang Jianmin Ma | 2020 | Chinese Chemical Letters2020，31，1： | 4 |
| 12 | Generation of tooth-like structures from integration-free human urine induced pluripotent stem cells显示文摘Background:Tooth is vital not only for a good smile,but also good health.Yet,we lose tooth regularly due to accidents or diseases.An ideal solution to this problem is to regenerate tooth with patients’own cells.Here we describe the generation of tooth-like structures from integration-free human urine induced pluripotent stem cells(ifhU-iPSCs).Results:We first differentiated ifhU-iPSCs to epithelial sheets,which were then recombined with E14.5 mouse dental mesenchymes.Tooth-like structures were recovered from these recombinants in 3 weeks with success rate up to 30%for 8 different iPSC lines,comparable to H1 hESC.We further detected that ifhU-iPSC derived epithelial sheets differentiated into enamel-secreting ameloblasts in the tooth-like structures,possessing physical properties such as elastic modulus and hardness found in the regular human tooth.Conclusion:Our results demonstrate that ifhU-iPSCs can be used to regenerate patient specific dental tissues or even tooth for further drug screening or regenerative therapies. | Jinglei Cai Yanmei Zhang Pengfei Liu Shubin Chen Xuan Wu Yuhua Sun Ang Li Ke Huang Rongping Luo Lihui Wang Ying Liu Ting Zhou Shicheng Wei Guangjin Pan Duanqing Pei | 2013 | Cell Regeneration2013，2，1： | 4 |
| 13 | Establishment and verification of a surgical prognostic model for cervical spinal cord injury without radiological abnormality显示文摘Some studies have suggested that early surgical treatment can effectively improve the prognosis of cervical spinal cord injury without radiological abnormality, but no research has focused on the development of a prognostic model of cervical spinal cord injury without radiological abnormality. This retrospective analysis included 43 patients with cervical spinal cord injury without radiological abnormality. Seven potential factors were assessed: age, sex, external force strength causing damage, duration of disease, degree of cervical spinal stenosis, Japanese Orthopaedic Association score, and physiological cervical curvature. A model was established using multiple binary logistic regression analysis. The model was evaluated by concordant profiling and the area under the receiver operating characteristic curve. Bootstrapping was used for internal validation. The prognostic model was as follows: logit(P) =-25.4545 + 21.2576 VALUE + 1.2160SCORE-3.4224 TIME, where VALUE refers to the Pavlov ratio indicating the extent of cervical spinal stenosis, SCORE refers to the Japanese Orthopaedic Association score(0–17) after the operation, and TIME refers to the disease duration(from injury to operation). The area under the receiver operating characteristic curve for all patients was 0.8941(95% confidence interval, 0.7930–0.9952). Three factors assessed in the predictive model were associated with patient outcomes: a great extent of cervical stenosis, a poor preoperative neurological status, and a long disease duration. These three factors could worsen patient outcomes. Moreover, the disease prognosis was considered good when logit(P) ≥-2.5105. Overall, the model displayed a certain clinical value. This study was approved by the Biomedical Ethics Committee of the Second Affiliated Hospital of Xi'an Jiaotong University, China(approval number: 2018063) on May 8, 2018. | Jie Wang Shuai Guo Xuan Cai Jia-Wei Xu Hao-Peng Li | 2019 | Neural Regeneration Research2019，14，4： | 4 |
| 14 | Fabrication and Characterization of Chitosan-Silk Fibroin/Hydroxyapatite Composites via in situ Precipitation for Bone Tissue Engineering显示文摘Homogeneous chitosan-silk fibroin/hydroxyapatite(CS-SF/HA) composites were prepared by in situ precipitation method driven by a multiple-order template. The morphology of the composites was investigated by scanning electron microscope(SEM) and transmission electron microscope(TEM). The compositional analysis was carried out by X-ray diffraction analysis(XRD) and Fourier transformed infrared spectroscopy(FTIR). The mechanical properties and biocompatibility of the composites were also determined. The results indicated that the inorganic particles of uniform size(50 nm) were well-dispersed among the CS-SF matrices. The compressive modulus of the CS-SF/HA composites was enhanced with the increasing amount of SF. The in vitro results suggested that the MC3T3-E1 osteoblast-like cells on CS-SF/HA composite disks displayed strong bonding and spreading, and the cell proliferation cultured on each composite disk increased throughout the culture period for up to 7 days. Especially, the samples with higher content of SF had much better biological properties. The evidences proved that the CS-SF/HA composites possessed excellent biocompatibility. By using the freeze-drying technique, hierarchical porous scaffolds with pores ranging from 50 μm to 200 μm were obtained. This work presented the advantages of in situ precipitation method to prepare the organic/inorganic composites, and a multiple-order template was introduced in the system to improve the properties of the composites by combining the merits of each organic template. | Jing-xiao Hu Xuan Cai Shao-bo Mo Li Chen Xin-yu Shen 童华 | 2015 | Chinese Journal of Polymer Science2015，33，12： | 3 |
| 15 | Differences in anti-inflammatory effects between two specifications of Scutellariae Radix in LPS-induced macrophages in vitro显示文摘Scutellariae Radix(SR), the root of Scutellaria baicalensis Georgi, is used as an antipyretic drug and has been demonstrated to have anti-inflammatory activity. SR is divided into two specifications, 'Ku Qin'(KQ) and 'Zi Qin'(ZQ), for use against different symptoms(upper energizer heat or lower portion of the triple energizer), according to the theory of traditional Chinese medicine(TCM). However, differences in the efficacies of these two specifications have not been determined. In the present study, we aimed to characterize the differences in the anti-inflammatory activities between KQ and ZQ and to explore how their differences are manifested in lipopolysaccharide(LPS)-induced macrophages. Our results showed that, in RAW264.7 cells(a mouse macrophage cell line derived from ascites), KQ and ZQ displayed anti-inflammatory effects by inhibiting the release of nitric oxide(NO), inducible NOS(iNOS), and nuclear factor-κB(NF-κB) in a dose-dependent manner without distinction. In NR8383 cells(a rat alveolar macrophage cell line), KQ and ZQ displayed similar effects on NO, iNOS, and NF-κB as seen in RAW264.7 cells, but KQ showed a higher inhibition rate for NO and i NOS than that shown by ZQ at the same concentration. These results indicated that there were differences in efficacy between KQ and ZQ in treating lung inflammation. Our findings provided an experimental evidence supporting the different uses of KQ and ZQ in clinic, as noted in ancient herbal records. | CHEN Qian-Yu WANG Chao-Qun YANG Zhi-Wei TANG Qi TAN Huan-Ran WANG Xuan CAI Shao-Qing | 2017 | Chinese Journal of Natural Medicines2017，15，7： | 3 |
| 16 | Utilization of H-bond interaction of nucleobase Uralic with antitumor methotrexate to design drug carrier with ultrahigh loading efficiency and pH-responsive drug release显示文摘A novel Uralic(U)-rich linear-hyperbranched mono-methoxy poly(ethylene glycol)-hyperbranched polyglycerol-graft-Uralic(mPEG-HPG-g-U)nanoparticle(NP)was prepared as drug carrier for antitumor methotrexate(MTX).Due to the H-bond interaction of U with MTX and hydrophobic interaction,this NP exhibited high drug loading efficiency of up to 40%,which was significantly higher than that of traditional NPs based on U-absent copolymers(<15%).In addition,MTX-loaded mPEG-HPG-g-U NPs also demonstrated an acidity-accelerated drug release behavior. | Teng-Teng Cai Qi Lei Bin Yang Hui-Zhen Jia Hong Cheng Li-Han Liu Xuan Zeng Jun Feng Ren-Xi Zhuo Xian-Zheng Zhang | 2014 | Regenerative Biomaterials2014，1，1： | 3 |
| 17 | Single-step flash-heat synthesis of red phosphorus/ graphene flame-retardant composite as flexible anodes for sodium-ion batteries显示文摘红磷(RP ) 为高效的 Na 离子电池作为阳极吸引了可观的注意,由于它的低费用和 2,600 mAh/g 的高理论特定的能力。在这研究,一个灵巧的单个步的闪光热处理被开发完成 graphene 氧化物的减小(去) 并且 RP 的同时的免职到减少的 graphene 氧化物(rGO ) 上表。产生 RP/rGO 合成被显示是克服在骑车期间与差的电子传导性和 RP 的大体积变化联系的期的一个有希望的候选人。RP/rGO 灵活电影阳极在 1 A/g 的控告 / 解除电流密度在 200 个周期期间交付了 1,625 mAh/g 的一个平均能力。在 1, 2, 4,和 6 A/g 当前的密度的 1,786, 1,597, 1,324,和 679 mAh/g 的平均电荷能力在率能力测试被获得。而且由于 RP 部件, RP/rGO 电影与一部 rGO 电影相比介绍了优异火焰 retardancy。这个工作因此介绍一个高度可存取的合成方法向 Na 离子存储与优异电气化学的性能准备灵活、安全的 RP 阳极。 | Yihang Liu Anyi Zhang Chenfei Shen Qingzhou Liu Jiansong Cai Xuan Cao Chongwu Zhou | 2018 | Nano Research2018，11，7： | 3 |
| 18 | AN EFFICIENT AND TARGET-ORIENTED SAMPLE ENRICHMENT METHOD FOR PREPARATIVE SEPARATION OF MINOR ALKALOIDS BY PH-ZONE-REFINING COUNTER-CURRENT CHROMATOGRAPHY显示文摘A sample enrichment method focusing on the minor targeted components was established to help them to be successfully separated by pH-zone refining CCC.Seven minor indole alkaloids in Uncaria rhynchophylla(Miq.)Miq.ex Havil(UR)were chosen to show the advantage of this method.The sample enrichment and separation were | Rui-Hong Feng Jin-Jun Hou Yi-Bei Zhang Hui-Qin Pan Peng Qi Shuai Yao Lu-Ying Cai Min Yang Baohong Jiang Xuan Liu Wan-Ying Wu De-An Guo | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 2 |
| 19 | An Area Weighted Surface Sampling Method for 3D Model Retrieval显示文摘3D model feature extraction is a key step for geometric content based 3D model retrieval. A 3D model is usually expressed by patches and it has large amount of data. Pseudo-random sequential Monte Carlo surface sampling method is used to sample 3D model data. It has strong randomness. The sampling results are not uniform, which worsen the efficiency and precision of feature extraction. An area weighted surface sampling method for 3D Models is proposed. The larger area patches get the higher probability of sampling. The sampling points are evenly distributed in sampling space, which represent more accurate 3D model features. Experiments show that the proposed method needs less computing resources. Even when there are less sampling points, it can still keep high calculation accuracy and stability. | LI Haisheng LIU Xuan LAI Long CAI Qiang DU Junping | 2014 | Chinese Journal of Electronics2014，23，3： | 2 |
| 20 | Improvement and Application of Acute Blood Stasis Rat Model Aligned with the 3Rs (Reduction, Refinement and Replacement) of Humane Animal Experimentation显示文摘Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while considering the 3Rs(reduction,refinement,and replacement)of humane animal experimentation.Methods:An acute blood stasis model was established in male Sprague-Dawley rats by subcutaneous injection(s.c.)Epi(1.2mg/kg)administration at 0h,followed by a 5-min exposure to an ice-bath at 2h and s.c.Epi administration at 4h.Control rats received physiological saline.Rats were fasted overnight and treated with Angelicae Sinensis Lateralis Radix(ASLR)and Pheretima the following day.Venous blood was collected using our novel cardiocentesis method and used to test whole blood viscosity(WBV),prothrombin time(PT),activated partial thromboplastin time(APTT),and fibrinogen(FIB)8ntent.Results:The rats survived the novel cardiocentesis technique;WBV value returned to normal while hematological parameters such as hemoglobin level and red blood cell count were restored to>94%of the corresponding values in normal rats following a 14-day recovery.Epi(1.2 mg/kg,s.c.)combined with a 5-min exposure to the ice bath replicated the acute blood stasis rat model and was associated with the highest WBV value.In rats showing acute blood stasis,ASLR treatment[4g/(kg-d)for 8 days]decreased WBV by 9.98%,11.09%,9.34%,9.00%,7.66%,and 7.03%(P<0.05),while Pheretima treatment[2.6g/(kg?d),for 8 days]decreased WBV by 25.49%,25.94%,16.28%,17.76%,11.07%,and 7.89%(P<0.01)at shear rates of 1,3,10,30,100,and 180 s'1,respectively.Furthermore,Pheretima treatment increased APTT significantly(P<0.01).Conclusions:We presented a stable,reproducible,and improved acute blood stasis rat model,which could be applied to screen drugs for promoting blood circulation and eliminating blood stasis. | HUANG Shuai XU Feng WANG Yin-ye SHANG Ming-ying WANG Chao-qun WANG Xuan CAI Shao-qing | 2020 | Chinese Journal of Integrative Medicine2020，26，4： | 2 |
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