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| 1 | Multicenter follow-up study of ankle fracture surgery显示文摘在从流行病学、临床的大 multicenter 的中国的脚关节破裂上的很少数据学习的背景是这研究的 available.The 目的是由考察在这研究包括了的 China.Methods 在五所医院经历了脚关节骨折外科的 235 个病人评估流行病学的特征和脚关节破裂的外科的结果经历了脚关节的病人折断外科在到2009年7月 .Age 的从2000年1月的五所中国医院,性,损害的机制, Arbeitsgemeinschaft f | XU Hai-lin LIU Li-min LI Xuan ZHANG Dian-ying FU Zhong-guo WANG Tian-bing ZHANG Pei-xun JIANG Bao-guo SHEN Hui-liang WANG Gang WANG Guang-lin WU Xin-bao | 2012 | Chinese Medical Journal2012，，4： | 27 |
| 2 | Homology-mediated end joining-based targeted integration using CRISPR/Cas9显示文摘 | Yao, Xuan Wang, Xing Hu, Xinde Liu, Zhen Liu, Junlai Zhou, Haibo Shen, Xiaowen Wei, Yu Huang, Zijian Ying, Wenqin Wang, Yan Nie, Yan-Hong Zhang, Chen-Chen Li, Sanlan Cheng, Leping Wang, Qifang Wu, Yan Huang, Pengyu Sun, Qiang Shi, Linyu Yang, Hui | 2017 | Cell Research2017，27，6： | 28 |
| 3 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 4 | Carbon Monoxide Alleviates Salt-Induced Oxidative Damage in Wheat Seedling Leaves显示文摘一氧化碳(公司),在降级期间释放的一个副产品他我在动物的 byheme 氧 ases ( EC 1.14.99.3 ),被认为是涉及最近作为在调停起一个主要作用被报导了的许多生物事件的一个重要生理的送信人 orbioactive 分子对导致氧化剂的肺损害的 cytoprotection 。在现在的学习,我们首先在小麦幼苗叶子对导致盐的氧化损坏决定了外长的公司的保护的效果。当 COdonor 表明了叶绿素的重要颠倒,与 0.01 微 mol/L 血色素对待的小麦幼苗腐烂,弄干重量,并且浇导致的 by300 mmol/L NaCl 强调的损失。有趣地,类脂化合物的增加被 0.01 nmol/L 血色素处理每在 salt-treatedleaves 观察的氧化颠倒。时间功课分析证明 0.01 微 mol/L 血色素的申请提高了 guaiacol 过氧化物酶,超级氧化物 dismutase,在小麦幼苗叶子的 ascorbate peroxidaseand 过氧化氢酶活动使遭到了腌应力。因为 CO scavenger 血红素(1.2 mg/L ) 堵住了 CO 施主血色素的行动,这些效果为公司是特定的。然而, CO 施主(1.0 微 mol/L ) 的更高的集中没减轻干燥重量和强调盐的小麦幼苗的水损失。这些结果建议一个公司施主的底层的外长的申请可能对咸度毒性是有益的…… | Ben-Kai Huang Sheng Xu Wei Xuan Ming LI Ze-Yu Cao Kai-Li Liu Teng-Fang Ling Wen-Biao Shen | 2006 | Journal of Integrative Plant Biology2006，48，3： | 20 |
| 5 | Carbon monoxide-induced adventitious rooting of hypo-cotyl cuttings from mung bean seedling显示文摘In this paper, the regulatory role of ex-ogenous carbon monoxide (CO) during the genera-tion and development of adventitious roots in mung bean (Phaseolus radiatus L. cv. Mingguang) hypo-cotyl cuttings was surveyed. The results indicated that, like nitric oxide (NO), CO donor Hematin in-duced adventitious rooting in dose- and time-de- pendent manners. These responses were also prov- en by the addition of gaseous CO aqueous solution with different concentrations. Additionally, the above CO-mediated effect was differently reversed when CO scavenger hemoglobin (Hb), CO specific syn-thetic inhibitor ZnPPIX, the auxin transport inhibitor NPA and nitric oxide synthase (NOS) inhibitor L-NAME were added with Hematin, respectively. Furthermore, after being treated with CO for 36 h, NO fluorescence was significantly enhanced and mainly distributed in the zone of vascular bundles whereas the specific NO scavenger cPTIO significantly inhib-ited NO fluorescence induced by CO, indicating that CO induces adventitious rooting of hypocotyl cuttings from mung bean seedling probably mediated by NO/NOS pathway. | XU Ji XUAN Wei HUANG Benkai ZHOU Yehua LING Tengfang XU Sheng SHEN Wenbiao | 2006 | Chinese Science Bulletin2006，51，6： | 16 |
| 6 | Involvement of carbon monoxide produced by heme oxygenase in ABA-induced stomatal closure in Vicia faba and its proposed signal transduction pathway显示文摘Carbon monoxide (CO) has recently proven to be an important bioactive or signaling molecule in mammalian cells. Its effects are mainly mediated by nitric oxide (NO) and cyclic GMP (cGMP). In Vicia faba leaves, CO production and heme oxygenase (HO) activity, an important CO synthetic enzyme, are first reported to increase in response to ABA treatment, which could result in stomatal closure. Inter- estingly, ABA-induced stomatal closure in V. faba guard cells is partially blocked when the synthetic CO inhibitor ZnPP, or the CO/NO scavenger Hb is added. Furthermore, we show that, exogenously applied CO donor, hematin, and CO aqueous solution not only result in the enhancement of CO release, but also time-dependently induce stomatal closure, and the latter is mimicked by the application of an NO donor SNP. The above-mentioned stomatal closure effects are differentially reversed by the addition of tungstate, a potent inhibitor of NO synthetic enzyme nitrate reductase (NR), the specific NO scavenger cPTIO, ZnPP, or Hb. During treatment for 4 h, SNP, 0.01% CO aqueous solution or hematin significantly triggers NO synthesis, whereas cPTIO, or tungstate approximately fully inhibits NO fluorescence. Ad- ditionally, application of the GC inhibitor ODQ blocks CO-induced stomatal closure. This inhibition could be reversed when 8-Br-cGMP is added. Thus, the above results suggest that CO produced by HO is involved in ABA-induced stomatal closure, and NO and cGMP may function as downstream interme- diates in the CO signaling responsible for stomatal closure. | CAO ZeYu HUANG BenKai WANG QingYa XUAN Wei LING TengFang ZHANG Bo CHEN Xi NIE Li SHEN WenBiaot | 2007 | Chinese Science Bulletin2007，52，17： | 16 |
| 7 | Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves显示文摘Pretreatment of lower H2O2 doses (0.05, 0.5 and 5 mM) for 24 h was able to dose-dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H2O2 (150 mM) for 6 h, with 0.5 mM H2O2 being the most effective concentrations. Further results illustrated that 0.5 mM H2O2 pretreatment triggered the biphasic production of H2O2 during a 24 h period. We also noticed that only peak I (0.25 h) rather than peak Ⅱ (4 h) was approximately consistent with the enhancement of heme oxygenase (HO) activity, HO-1 gene expression. Meanwhile, enhanced superoxide dismutase (SOD) activity, Mn-SOD and Cu,Zn-SOD transcripts might be a potential source of peak I of endogenous H2O2. Further results confirmed that 0.5 mM H2O2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi-quantitative reverse transcription-polymerase chain reaction and western blotting. Meanwhile, the application of N,N'-dimethylthiourea, a trap for endogenous H2O2, not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H2O2 production (peak I) plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress. | Xiao-Yue Chen Xiao Ding Sheng Xu Ren Wang Wei Xuan Ze-Yu Cao Jian Chen Hong-Hong Wu Mao-Bing Ye Wen-Biao Shen | 2009 | Journal of Integrative Plant Biology2009，51，10： | 14 |
| 8 | Arctigenin enhances swimming endurance of sedentary rats partially by regulation of antioxidant pathways显示文摘 | Ruo-ming WU Yan-yan SUN Ting-ting ZHOU Zhi-yuan ZHU Jing-jing ZHUANG Xuan TANG Jing CHEN Li-hong HU Xu SHEN | 2014 | Acta Pharmacologica Sinica2014，35，10： | 13 |
| 9 | X-ray image characteristics and related measurements in the ankles of 118 adult patients with Kashin-Beck disease显示文摘 | Zeng Yi Zhou Zongke Shen Bin Yang Jing Kang Pengde Zhou Xuan Zou Ling Pei Fuxing | 2014 | Chinese Medical Journal2014，，13： | 13 |
| 10 | A randomized, multicenter controlled trial to compare the efficacy of recombinant human parathyroid hormone (1-34) with elcatonin in postmenopausal women with osteoporosis in China显示文摘背景 Recombinant 人的甲状旁腺的荷尔蒙(1-34 )(rhPTH (注射给的 1 -34)) 一新生物产品的第七班药,它被采用基因再结合准备技术。rhPTH (1 -34) 主要被用来对待骨质疏松症,特别为绝经后的 women.This,学习为在中国的 11 个城市的区域与骨质疏松症对待绝经后的女人把 rhPTH (1-34 ) 的临床的功效和安全与 elcatonin 作比较。有骨质疏松症的 205 个女人被注册在的方法一 6 月, multicenter,使随机化的、控制学习。他们被使随机化收到任何一个 rhPTH (1-34 ) 20 ug (200 U ) 日报或 elcatonin 20 U 周刊。腰部的脊骨(L1-4 ) 和大腿骨的颈骨头矿物质密度(BMD ) ,以及骨头周转的生物化学的标记被测量。不利事件是增加的 recorded.Results rhPTH (1-34 ) 腰部的 BMD 非常显著地在 3 个月和 6 个月做了 elcatonin (2.38% 对 0.59% , P < 0.05;5.51% 对 1.55% , P < 0.01 ) ,但是没有在在大腿骨的颈的这二个组的 BMD 的重要增加。在 rhPTH 的骨头标记有更大的吝啬的增加(1 个 -34) 组比在在 3 个月和 6 个月的 elcatonin 组(浆液骨头特定的碱的磷酸酶(BSAP )36.79% 对 0.31% ;92.42% 对 -0.17% ;尿 N-telopeptide/creatinine (NTX/Cr )48.91% 对 -5.32% ;68.82% 对 -10.86%) 。处理很好被容忍,没有重要差别,在任何不利事件和任何严肃的不利事件的比例在二个组之间检测(67.0% 对 59.0% ;0 对 0 ).Conclusions rhPTH (1-34 ) 比 elcatonin 在骨头形成上有更积极的效果,由腰部的 BMD 和骨头形成标记的更大的增长出现与在肝的仅仅温和的不利事件和没有重要变化,肾或 hematological 索引。 | ZHANG Xiu-zhen WANG Bo YANG Jun XUAN Miao SONG Li-ge LI Hong GUO Xiao-hui LU Xiao-feng XUE Qing-yun YANG Gang-yi JI Qiu-he SHEN Jie LIU Zhi-min LI Cheng-jiang WU Tian-feng TONG Xiao-cui JIA Yuan | 2009 | Chinese Medical Journal2009，，24： | 12 |
| 11 | Effects of tensile forces on serum deprivation-induced osteoblast apoptosis: expression analysis of caspases, Bcl-2, and Bax显示文摘 | LI Xuan ZHANG Xiao-ling SHEN Gang TANG Guo-hua | 2012 | Chinese Medical Journal2012，，14： | 12 |
| 12 | Ultrathin Al2O3-coated reduced graphene oxide membrane for stable lithium metal anode显示文摘Lithium(Li) metal has been considered as the most attractive anode materials for Li-ion batteries(LIBs)due to its high theoretic specific capacity. The formation of unstable solid electrolyte interphase(SEI) and dendritic Li on the metal anode, however, hindered its practical application. Herein, to address the issues, a Li-free electrode with ultrathin Al_2O_3 coated on reduced graphene oxide(rGO) membrane that covers a Cu foil current collector was developed. The composite electrode exhibits excellent interfacial protection of lithium metal deposited between Cu foil and rGO electrochemically. Firstly, it affords good Li^+ permeability from the electrolyte. Secondly, the ultrathin Al_2O_3 has sufficient mechanical strength to inhibit the penetration of Li dendrite. Li metal was observed uniformly deposited between rGO membrane and Cu collector, and stable cycle performance of Li plating/stripping with Coulombic efficiency of ~91.75% at the 100 th cycle is achieved in organic carbonate electrolyte without any additives. | Fan Zhang Fei Shen ZhaoYang Fan Xin Ji Bin Zhao ZhouTing Sun YingYing Xuan XiaoGang Han | 2018 | Rare Metals2018，37，6： | 11 |
| 13 | Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence显示文摘Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of 'Darmor-bzh'and 'Tapidor,'respectively.We generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of B.napus by calculating the genetic diversity and linkage disequilibrium parameters.Selective-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding. | Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li Imran Haider Shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang | 2019 | Molecular Plant2019，12，1： | 10 |
| 14 | Risk factors for hepatic decompensation in patients with primary biliary cirrhosis显示文摘AIM:To examine the clinical features and analyze prognostic factors in a prospective study of primary biliary cirrhosis(PBC) patients.METHODS:From 1995 to 2010,PBC patients without hepatic decompensation seen at the Peking Union Medical College Hospital were enrolled.Clinical signs and manifestations(pruritus,persistent fatigue,jaundice and pain in the right hypochondrium),laboratory parameters(auto-antibodies for autoimmune hepatic disease,biliary and hepatic enzymes,immunoglobulin,bilirubin,and albumin) and imaging findings were recorded at entry and at specific time points during follow-up.Cox regression and Kaplan-Meier analyses,respectively,assessed the risk factors for hepatic decompensation and survival.RESULTS:Two hundred and sixty-two PBC patients were enrolled with a median follow-up of 75.2 mo(range,21-201 mo).The 240 patients were aged 51.5 ± 10.2 years at diagnosis and 91.6% were female.Two hundred and forty-five(93.5%) were seropositive for anti-mitochondrial antibodies.At presentation,170 patients(64.9%) were symptomatic,while 96 patients(36.6%) had extra-hepatic autoimmune disease.During the follow-up period,62(23.7%) patients developed hepatic decompensation of whom four underwent liver transplantation and 17 died.The cumulative survival rate and median survival time were 83.9% and 181.7 mo,respectively.Cox regression analysis revealed that an incomplete ursodeoxycholic acid(UDCA) response or inconsistent treatment [P < 0.001;hazard risk(HR) 95%CI = 2.423-7.541],anti-centromere antibodies(ACA) positivity(P < 0.001;HR 95%CI = 2.516-7.137),alanine aminotransferase ratio(AAR) elevations(P < 0.001;HR 95%CI = 1.357-2.678),and histological advanced liver disease(P = 0.006;HR 95%CI = 1.481-10.847) were predictors of hepatic decompensation.The clinical features and survival of PBC in China are consistent with those described in Western countries.CONCLUSION:Incomplete UDCA response or inconsistent treatment,ACA positivity,AAR elevations,and advanced histological stage are predictors of decompensation. | Tian-Yan Shi Li-Na Zhang Hua Chen Li Wang Min Shen Xuan Zhang Feng-Chun Zhang | 2013 | World Journal of Gastroenterology2013，19，7： | 9 |
| 15 | Is urban development an urban river killer? A case study of Yongding Diversion Channel in Beijing, China显示文摘The high population and concrete environment alter urban areas by changing temperature, rainfall runoff, and water resource utilization activities. This study was conducted to investigate the water quality features of the Yongding Diversion Channel in Beijing, China, and its relationship with rainfall and urban development. Monthly water quality data were obtained from April to October of 2004 at monitoring sites of Sanjiadian, Gaojing, Luodaozhuang, and Yuyuangtan. The monthly water quality grades from 2007 to 2011 were also investigated and compared with those of other rivers. Dissolved oxygen and pH showed greater decreases after one or two moderate rainfall events than several light rainfall events. The potassium permanganate index(CODMn), ammonia nitrogen(NH3-N) and total phosphorus(TP) increased more after several light rainfall events than after one or two moderate or heavy rainfall events. Pollutant concentrations(CODMn, NH3-N, TP) in downstream regions showed greater changes than those in upstream areas after heavy rainfall events. Intense human activities around the channel greatly influenced the water quality of the channel in rainy season because of runoff pollution; however, heavy rainfall had a strong dilution effect on the pollutant concentrations in rivers. Overall, urban development has obviously deteriorated the water quality of the Yongding Diversion Channel as indicated by an increase in the water quality index from 3.22 in 2008 to 4.55 in2010. The Pearson correlation between monthly rainfall and water quality indices from 2007 to 2011ranged from 0.1286 to 0.6968, generally becoming weaker as rainfall and rainfall runoff became more random and extreme. | Xi Wang Junqi Li Yingxia Li Zhenyao Shen Xuan Wang Zhifeng Yang Inchio Lou | 2014 | Journal of Environmental Sciences2014，26，6： | 8 |
| 16 | Resveratrol protects PC12 cells against OGD/ R-induced apoptosis via the mitochondrial-mediated signaling pathway显示文摘在这研究,我们对导致的氧葡萄糖 deprivation/reoxygenation (OGD/R ) 调查了 resveratrol 的 neuroprotective 潜力在区分得好的 PC12 房间和内在的机制的 apoptotic 损坏。房间当面在正常状况或 OGD/R 下面被孵化或 10 M resveratrol 的缺席。房间生存能力与 methyl-thiazolyl-tetrazolium (MTT ) 被决定试金。Apoptotic 比率与 Hoechst 33342 染色和 Annexin V-FITC/PI 两倍染色被决定。氧化应力被测量细胞内部的反应的氧评估 superoxide dismutase (草皮) 和过氧化氢酶(猫) 的种类(ROS ) , mitochondrial superoxide, malondialdehyde (MDA ) 内容,和这些活动。细胞内部的钙([Ca 2+]i) 被 Fluo-3/AM 估计。mitochondrial 膜潜力(MMP ) 被 5,5,6,6-tetrachloro-1,1,3,3-tetraethyl-benzimidazolyl-carbocyanine 碘化物(JC-1 ) 和玫瑰精评估 123 (Rh123 ) 。mitochondrial 渗透转变毛孔(MPTP ) 的开始被 Calcein/Co 2+-quenching 技术决定。细胞色素 c, Bcl-2, Bax,劈开的 caspase-9,和劈开的 caspase-3 的蛋白质层次被西方的污点分析检测。结果证明 10 M resveratrol 稀释了 OGD/R-induced 房间生存能力损失和房间 apoptosis,它在在 SOD 和猫活动的 MDA 内容和增加与减少被联系。而且,细胞内部的 ROS 和 mitochondrial superoxide 的累积,骚乱[Ca 2+]i 动态平衡, MMP 的减小, MPTP 的开始,和在 OGD/R-injured 房间观察的 mitochondrial 细胞色素 c 的版本,在调停 mitochondrial 的 apoptotic 小径上显示了一个开关,都被 resveratrol 颠倒。这些结果建议 resveratrol 管理可以经由 modulating 起一个 neuroprotective 作用在 OGD/R-induced PC12 房间损害的调停 mitochondrial 的发信号小径。 | Xuan Liu Xiangyang Zhu Miao Chen Qinmin Ge Yong Shen Shuming Pan | 2016 | Acta Biochimica et Biophysica Sinica2016，48，4： | 8 |
| 17 | 针栓式喷注器单元液/液撞击的一次破碎形态的仿真研究显示文摘目的:针对液/液针栓式喷注器单元,研究其在不同径向射流喷注速度下的一次破碎形态,并阐明在一次破碎下喷雾半锥角的形成、压力场分布、喷雾粒径分布与速度场分布。创新点:通过流体体积函数转换离散相(VOF-to-DPM)模型,结合网格自适应(AMR)技术还原了针栓式喷注器单元液/液撞击的一次破碎形态。方法:1.通过VOF-to-DPM模型完成一次破碎过程中对液相的捕捉;2.采用计算流体动力学后处理(CFD-post)模块进行后处理,得到一次破碎下喷雾半锥角的形成以及压力场、喷雾粒径与速度场的分布云图;3.在仿真计算过程中使用AMR技术减少计算量,节约时间成本与计算资源。结论:1.速度大的径向射流在穿透轴向液膜后会形成一个蘑菇状的头部;扰动在蘑菇状顶端下方形成涡,有助于蘑菇状顶端边缘破碎的发生。2.气动力和表面张力对轴向液膜破碎过程中产生的直液丝和环状液丝的破碎起到重要作用;由于液滴的聚合现象,轴向液膜的直径在破碎过程中并不是单调递减的。3.喷雾半锥角的大小和径向射流速度的大小成正比;一次破碎首先发生在轴向液膜前沿、径向射流头部以及撞击点附近。4.在表面张力的作用下,轴向液膜内边缘的速度较小,直径较大;当径向射流的速度增大时,轴向液膜内边缘的速度值减小得更加明显。 | Rui ZHOU Chi-bing SHEN Xuan JIN | 2020 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2020，21，8： | 8 |
| 18 | Exogenous Hematin Alleviates Mercury-induced Oxidative Damage in the Roots of Medicago sativa显示文摘在现在的学习,我们评估了保护的效果外长他我 oxygenase-1 (HO-1 EC 1.14.99.3 ) 对在苜蓿(Medicago sativa L.) 的根的导致水银的氧化损坏的 inducer 血色素。暴露于水银(HgCl ) 的植物每根延伸的氧化,以及抑制展出了类脂化合物的重要增加。然而,到对待的 HgCl (100 omol/L ) 的补充有效地种的血色素(50 oM ) 每氧化减少了类脂化合物并且部分增加了根延伸。这些回答被一氧化碳(公司) 的水溶液的申请与 50% 浸透模仿。另外,有血色素的处理能在根纸巾导致 HO-1 抄本的有势力正式就职,作为检测 12 h 列在后面处理。而且,抗氧化剂酶,包括的谷胱甘肽 reductase , monodehydroascorbate reductase 和超级氧化物 dismutase 活动的激活,和 lipoxygenase 活动的减少,被血色素在 12 h 或 24 h 导致,它被每膜正直的氧化和损失的为类脂化合物的察觉的组织化学的染色进一步证实。而, ascorbate 过氧化物酶和 guaiacol 过氧化物酶 isozyme 活动或他们的抄本被减少分别地,显示那过氧化氢可能充当一个信号调停在处理的开始的 Hg 忍耐。血色素的改善效果是特定的,自从 CO scavenger 血红素差别颠倒了上述行动。总起来说,我们的结果建议血色素在保护植物免于导致 Hg 的氧化损坏展出一个重要角色。 | Yi Han Wei Xuan Tian Yu Wen-Bing Fang Tian-Ling Lou Yin Gao Xiao-Yue Chen XiaoXiao Wen-Biao Shen | 2007 | Journal of Integrative Plant Biology2007，49，12： | 7 |
| 19 | Carbon Monoxide Alleviates Wheat Seed Germination Inhibition and Counteracts Lipid Peroxidation Mediated by Salinity显示文摘最近在动物,内长的碳一氧化物(公司) 象氮的氧化物一样,作为另一个重要生理的送信人或 bioactive 分子被含有。然而,很少信息都不在整个植物对公司的生理的角色被知道。在现在的学习,我们报导 CO 施主血色素(0.1, 1.0 和 10.0 mol/L ) 的不同集中减轻了小麦(Triticum aestivum L。Yangmai 158 ) 种子萌芽抑制以一种剂量依赖者方式由 250 mmol/L NaCl 应力引起了。这些回答被不同气体的公司水的答案的增加也从 0.1% ~ 100.0% 浸透证明。在这些处理之中, 1.0 mol/L 血色素和公司水的答案的 1.0% 浸透的效果是最明显的。与非血色素处理相比,而且,在小麦种子的存储储备的降级也被加速。时间功课分析证明血色素的申请 dose-dependently 增加了 superoxide dismutase,过氧化氢酶, ascorbate peroxidase,和 guaiacol peroxidase 活动的活动,因此减少在发芽小麦种子的类脂化合物 peroxidation 使遭到了腌应力。同时,因为 CO scavenger 血红素(0.2 g/L ) 的增加堵住了 1.0 mol/L 血色素的各种各样的行动,血色素的回答为公司是特定的。一起拿,现在的学习的结果证明在低集中,公司能稀释咸度应力生产的种子萌芽抑制并且在发芽小麦种子抵抗类脂化合物 peroxidation。 | Sheng Xu Zhi-Sheng Sa Ze-Yu Cao Wei Xuan Ben-Kai Huang Teng-Fang Ling Qiong-Ying Hu Wen-Biao Shen | 2006 | Journal of Integrative Plant Biology2006，48，10： | 7 |
| 20 | Carbon Monoxide Promotes Lateral Root Formation in Rapeseed显示文摘一氧化碳(公司) ,无臬、无味、无色的气体,最近证明了在哺乳动物的房间是活跃的一份重要简历或信号分子,主要由氮的氧化物与它的效果调停了(没有) 。在现在的报告,我们证明外长的公司导致侧根(LR ) 形成,一个没有依赖的过程。到油菜籽的 CO 施主血色素的管理(Brassicanapus L。Yangyou 6 ) 为 3 天的幼苗, dose-dependently 支持了全部的长度和 numberofLRs。这些回答也被看见跟随不同浸透的集中的气体的公司水溶液的应用程序。而且,幼苗上的公司的行动充分被颠倒 CO scavenger 血红素(Hb ) 或公司特定的合成禁止者锌 protoporphyrin-IX (ZnPPIX ) 什么时候被增加。有趣地,它的特定的scavenger2-( 4-carboxyphenyl ) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide 钾腌的内长的 NO using ( cPTIO )的弄空或氮的氧化物 synthase ( NOS )禁止者 N^G-nitro-L-arginine 甲基酉旨(L名字),导致了完全的废除 ofLR 发展,说明一个重要角色为内长不在 LR 上的公司的行动形成。然而,由 200 亩 mol/L 钠 nitroprusside (SNP ) 的 LR 开发的正式就职,一没有施主,没被 ZnPPIX 的存在或缺席影响。而且,使用一条解剖途径与与没有特定的 fluorophore4,5-diaminofluorescein diacetate 扫描共焦的显微镜学的激光结合了,我们观察到血色素和 SNP 与控制样品增加了 NOreleasecompared 并且 NO 信号主要在 LR primordia (LRP ) 被散布,特别在 36 h 处理以后。LRP 被发现在控制有类似的形态学,对待 SNP 、对待血色素的幼苗。同样, NO 的改进由在 36 h 的公司的信号是 cPTIO, L 名字, ZnPPIX 和 Hb 的增加熄灭的差别。相反,正式就职不由 SNP 引起了没被 ZnPPIX 的申请影响。因此,我们进一步推断公司导致 LR 形成可能调停了由没有 / 没有小径并且可以不 COsignaling 下游地行动,它也被显示了发生在动物。 | Ze-Yu Cao Wei Xuan Zhao-Yang Liu Xin-Na Li Nan Zhao Peng Xu Zhe Wang Rong-Zhan Guan Wen-Biao Shen | 2007 | Journal of Integrative Plant Biology2007，49，7： | 7 |
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