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| 1 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 2 | Development of mouse preimplantation embryos in space显示文摘The development o f life beyond planet Earth is a long-standing quest of the human race,but whether normal mammalian embryonic development can occur in space is still unclear.Here,we show unequivocally that preimplantation mouse embryos can develop in space;but the rate of blastocyst formation and blastocyst quality are compromised.Additionally,the cells in the embryo contain severe DNA damage,while the genome of the blastocysts developed in space is globally hypomethylated with a unique set of differentially methylated regions.The developmental defects,DNA damage and epigenetic abnormalities can be largely mimicked by the treatment with ground-based low-dose radiation.However,the exposure to simulated microgravity alone does not cause major disruptions of embryonic development,indicating that radiation is the main cause for the developmental defects.This work advances the understanding of embryonic development in space and reveals long-term extreme low-dose radiation as a hazardous factor for mammalian reproduction. | Xiaohua Lei Yujing Cao Baohua Ma Yunfang Zhang Lina Ning Jingjing Qian Liwen Zhang Yongcun Qu Tao Zhang Dehong Li Qi Chen Junchao Shi Xudong Zhang Chiyuan Ma Ying Zhang Enkui Duan | 2020 | National Science Review2020，7，9： | 8 |
| 3 | Cryotherapy for cirrhosis-based hepatocellular carcinoma: a single center experience from 1595 treated cases显示文摘Cryoablation 是为 hepatocellular 癌(HCC ) 的不太流行的经皮的夺格的治疗,并且关于它的实用性的当前的证据是有限的。我们在与经皮的 cryoablation 对待 1595 个 HCC 案例关于有效性,安全和这治疗的长期的结果给全面侧面报导我们的经验。从 2003 年 1 月到 2013 年 12 月,有 2313 个 HCC 小瘤的 1595 个病人在我们的中心与 2958 个 cryoablation 会议被切除。完全的脱离与 3.4 朠湥浯捩椠獮慴楢楬祴 ? 畢 ? 的一条吝啬的直径为 1893 个小瘤在 1294 个病人被完成 ?? 潣扭湩摥眠瑩 ??? 瑡整畮瑡摥琠敨攠瑸湥 ? 景朠湥浯捩椠獮慴楢楬祴椠 ? 牰浩牡 ? 塖′畴潭 ? 楴獳敵瑡吗?? | Guanghua Rong Wenlin Bai Zheng Dong Chunping Wang Yinying Lu Zhen Zeng Jianhui Qu Min Lou Hong Wang Xudong Gao Xiujuan Chang Linjing An Yan Chen Yongping Yang | 2015 | Frontiers of Medicine2015，9，1： | 7 |
| 4 | SARS-CoV-2 infection aggravates chronic comorbidities of cardiovascular diseases and diabetes in mice显示文摘Background:Cardiovascular diseases(CVDs)and diabetes mellitus(DM)are top two chronic comorbidities that increase the severity and mortality of COVID-19.However,how SARS-CoV-2 alters the progression of chronic diseases remain unclear.Methods:We used adenovirus to deliver h-ACE2 to lung to enable SARS-CoV-2 infection in mice.SARS-CoV-2’s impacts on pathogenesis of chronic diseases were studied through histopathological,virologic and molecular biology analysis.Results:Pre-existing CVDs resulted in viral invasion,ROS elevation and activation of apoptosis pathways contribute myocardial injury during SARS-CoV-2 infection.Viral infection increased fasting blood glucose and reduced insulin response in DM model.Bone mineral density decreased shortly after infection,which associated with impaired PI3K/AKT/mTOR signaling.Conclusion:We established mouse models mimicked the complex pathological symptoms of COVID-19 patients with chronic diseases.Pre-existing diseases could impair the inflammatory responses to SARS-CoV-2 infection,which further aggravated the pre-existing diseases.This work provided valuable information to better understand the interplay between the primary diseases and SARS-CoV-2 infection. | Yuanwu Ma Dan Lu Linlin Bao Yajin Qu Jiangning Liu Xiaolong Qi Lei Yu Xu Zhang Feifei Qi Qi Lv Yunpeng Liu Xudong Shi Caixian Sun Jing Li Jie Wang Yunlin Han Kai Gao Wei Dong Ning Liu Shan Gao Jing Xue Qiang Wei Sidan Pan Hong Gao Lianfeng Zhang Chuan Qin | 2021 | Animal Models and Experimental Medicine2021，4，1： | 3 |
| 5 | Puromycin A, B and C, cryptic nucleosides identified from Streptomyces alboniger NRRL B-1832 by PPtase-based activation显示文摘Natural product discovery is pivot for drug development,however,this endeavor is often challenged by the wide inactivation or silence of natural products biosynthetic pathways.We recently developed a highly efficient approach to activate cryptic/silenced biosynthetic pathways through augmentation of the phosphopantetheinylation of carrier proteins.By applying this approach in the Streptomyces alboniger NRRL B-1832,we herein identified three cryptic nucleosides products,including one known puromycin A and two new derivatives(puromycin B and C).The biosynthesis of these products doesn't require the involvement of carrier protein,indicating the phosphopantetheinyl transferase(PPtase)indeed plays a fundamental regulatory role in metabolites biosynthesis.These results demonstrate that the PPtasebased approach have a much broader effective scope than the previously assumed carrier proteininvolving pathways,which will benefit future natural products discovery and biosynthetic studies. | Xiaoli Yan Benyin Zhang Wenya Tian Qi Dai Xiaoqin Zheng Ke Hu Xinxin Liu Zixin Deng Xudong Qu | 2018 | Synthetic and Systems Biotechnology2018，3，1： | 3 |
| 6 | Microwave triggered multifunctional nanoplatform for targeted photothermal-chemotherapy in castration-resistant prostate cancer显示文摘Lacking a precise targeting strategy,castration-resistant prostate cancer(CRPC)is still hard to be treat effectively.Exploring treatment options that can accurately target CPRC is an important issue with urgent need.In this study,a novel nanotechnologybased strategy had been developed for the precise target treatment of CRPC.By combining microwaves and photothermal therapy(PTT),this nanoplatform,cmHSP70-PL-AuNC-DOX,targets tumor tissues with outstanding precision and achieves better anti-tumor activity by simultaneously eliciting photothermal and chemotherapeutic effects.From nanotechnology,cmHSP70-modified and thermo-sensitive liposome-coated AuNC-DOX were prepared and used for CRPC-targeted photothermal ablation and chemotherapy.Doxorubicin(DOX)was selected as the chemotherapeutic agent for cytotoxicity.In terms of the curative scheme,prostate tissues were firstly pre-treated with microwaves to induce the expression of heat shock protein 70(HSP70)and its migration to the cell membrane,which was then targeted by HSP70 antibody(cmHSP70)coated on the nanoparticles to achieve accurate drug delivery.The nanoplatform then achieved precise ablation and controlled release of DOX under external near-infrared(NIR)irradiation.Through the implementation,the targeting,cell killing,and safety of this therapeutical strategy had been verified in vivo and in vitro.This work establishes an accurate,controllable,efficient,non-invasive,and safe treatment platform for targeting CRPC,provides a rational design for CRPC’s PTT,and offers new prospects for nanomedicines with great precision. | Pengyuan Liu Yajun Wu Xiaogang Xu Xudong Fan Chuan Sun Xiaojie Chen Jindan Xia Shiting Bai Li Qu Huasong Lu Jing Wu Jun Chen Ji-Gang Piao Zhibing Wu | 2023 | Nano Research2023，16，7： | 2 |
| 7 | Efficient editing DNA regions with high sequence identity in actinomycetal genomes by a CRISPR-Cas9 system显示文摘Actinobacteria able to produce varieties of bioactive natural products have been long appreciated by the field of drug discovery and development.Recently,a few of CRISPR/Cas9 systems bearing different types of replicons(pSG5 and pIJ101)were developed to efficiently edit their genomes.Despite wide application in gene editing,their utility in editing challenging DNA regions e.g.high sequence identity has not been compared.In this study,we confirmed that the widely used temperature-sensitive pSG5 replicon is indeed not suitable for editing modular polyketide synthase(PKS)genes due to causing unpredicted gene recombination.This problem can be addressed by replacing the pSG5 with the segregationally unstable pIJ101 replicon.By introducing a counterselection marker CodA,convenient cloning sites in the single guide RNAs(sgRNAs)and homologous template scaffolds,we developed a new CRISPR-Cas9 system pMWCas9.This system was successfully used to delete/replace erythromycin PKS and other biosynthetic genes in Saccharopolyspora erythraea and Streptomyces sp.AL2110.By swapping the promoters of antB and antC with ermE and kasOp,we achieved a deacyl-antimycin hyper producer which produces a 9-fold higher yield than the original Streptomyces sp.AL2110 strain.Our results provide a robust and useful Cas9 tool for genetic studies in Actinobacteria. | Jingjun Mo Shuwen Wang Wan Zhang Chunyu Li Zixin Deng Lixin Zhang Xudong Qu | 2019 | Synthetic and Systems Biotechnology2019，4，2： | 2 |
| 8 | Fluorescence quenching of TMR by guanosine in oligonucleotides显示文摘Nucleotide-specific fluorescence quenching in fluorescently labeled DNA has many applications in biotechnology. We have studied the inter-and intra-molecular quenching of tetramethylrhodamine (TMR) by nucleotides to better understand their quenching mechanism and influencing factors. In agreement with previous work, dGMP can effectively quench TMR, while the quenching of TMR by other nucleotides is negligible. The Stern-Volmer plot between TMR and dGMP delivers a bimolecular quenching constant of Ks=52.3 M-1. The fluorescence of TMR in labeled oligonucleotides decreases efficiently through photoinduced electron transfer by guanosine. The quenching rate constant between TMR and guanosine was measured using fluorescence correlation spectroscopy (FCS). In addition, our data show that the steric hindrance by bases around guanosine has significant effect on the G-quenching. The availability of these data should be useful in designing fluorescent oligonucleotides and understanding the G-quenching process. | QU Peng CHEN XuDong ZHOU XiaoXue LI Xun ZHAO XinSheng | 2009 | Science China Chemistry2009，52，10： | 1 |
| 9 | Endovascular Placement of Iodine-125 Seed Strand and Stent Combined with Chemoembolization for Treatment of Hepatocellular Carcinoma with Tumor Thrombus in Main Portal Vein显示文摘 | JianJun Luo ZhiPing Yan QinXin Liu XuDong Qu JianHua Wang | 2011 | Journal of Vascular and Interventional Radiology2011，，4： | 1 |
| 10 | Transcatheter arterial embolization-salvaged ALPPS, a novel ALPPS procedure especially for patients with hepatocellular carcinoma and severe fibrosis/cirrhosis显示文摘Background:The associating liver partition and portal vein ligation for staged hepatectomy(ALPPS)for hepatocellular carcinoma(HCC)with fibrosis/cirrhosis is often associated with limited growth of future liver remnant(FLR).We introduced a new procedure named transcatheter arterial embolization-salvaged ALPPS(TAE-salvaged ALPPS)which was shown to be especially suitable for HCC patients with cirrhosis or fibrosis who failed adequately to respond to conventional ALPPS.The short-term efficacy and safety for the TAE-salvaged ALPPS on patients with HCC and fibrosis/cirrhosis were studied.Methods:Consecutive HCC patients who underwent TAE-salvaged ALPPS in our hospital between November 2016 and June 2020 were retrospectively studied.The new ALPPS procedure included conventional ALPPS stage-1 using associating liver partition and portal vein ligation.When FLR failed to reach sufficient hypertrophy,TAE was carried out 2 weeks later followed by liver resection 3 weeks after ALPPS stage-1.Results:Nine of 10 patients had a single tumor(median diameter 14.0 cm,range,5.2-17 cm).The remaining patient had multiple tumors(diameter of one tumor 14.0 cm,and two satellite foci 2.0 and 3.0 cm).R0 resection was achieved in all patients(100%)after a median of 21 days.Six patients had cirrhosis,1 had METAVIR grade-3 fibrosis,and 3 had METAVIR grade-2 fibrosis.The median increase in FLR volume after TAE-salvaged ALPPS was 69.7%(34.4-143.9%).The absolute and relative kinetic growth rates(KGRs)were 9.9(7.1-17.3)mL/day and 3.4%(1.9-7.2%)/day,respectively.The median absolute KGRs were 15.7,2.6,and 19.5 mL/day in the first,second,and third postoperative weeks after ALPPS stage-1,respectively.The rapid increase in KGR on the third week was induced by TAE.The overall postoperative morbidity rates were 50,0%(5/10),20.0%(2/10)and 70.0%(7/10)after ALPPS stage-1,TAE and ALPPS stage-2,respectively.The 90-day mortality rate was 10.0%(1/10).The median overall survival was 40 months.Conclusions:The new TAE-salvaged ALPPS induced significant increases in FLR volumes within 3 weeks in patients with HCC and fibrosis/cirrhosis.The procedure is promising in treating patients with HCC and fibrosis/cirrhosis who fail to achieve sufficient FLR hypertrophy after conventional ALPPS stage-1. | Yuanfei Peng Zheng Wang Xudong Qu Feiyu Chen Huichuan Sun Xiaoying Wang Zhenbing Ding Min Tang Lei Yu Xinrong Yang Qiang Gao Zhaoyou Tang Wan Yee Lau Jia Fan Jian Zhou | 2022 | Hepatobiliary Surgery and Nutrition2022，11，4： | 1 |
| 11 | Endovascular Placement of Iodine-125 Seed Strand and Stent Combined with Chemoembolization for Treatment of Hepatocellular Carcinoma with Tumor Thrombus in Main Portal Vein显示文摘 | JianJun Luo ZhiPing Yan QinXin Liu XuDong Qu JianHua Wang | 2011 | Journal of Vascular and Interventional Radiology2011，，4： | 1 |
| 12 | Structure-guided protein design of fluoroacetate dehalogenase for kinetic resolution of rac-2-bromobutyric acid显示文摘R-2-Bromobutyric acid is a very important intermediate for the synthesis of agrochemicals and pharmaceuticals.Bioresolution of rac-2-bromobutyric acid(rac-2-BBA)provides a promising process for R-2-bromobutyric acid(R-2-BBA)production.The fluoroacetate dehalogenase(FAcD)has been always studied in the defluorination process.We found that FAcD RPA1163 showed detectable activity but no enantioselectivity towards rac-2-BBA.The iterative saturation mutagenesis(ISM)of FAcD RPA1163 resulted in a mutant H155V/W156R/Y219M,which catalyzed the kinetic resolution of rac-2-BBA to produce R-2-BBA with enhanced activity and enantioselectivity(99.3%ee).The high preference for S-2-bromobutyric acid(S-2-BBA)is of synthetic value.Molecular docking analysis indicated that the H155V/W156R/Y219M mutation reduced steric hindrance and broadened the halide pocket.It is not only the steric hindrance but also the electrostatic environment that has an effect on the activity and enantioselectivity. | Shu Wang Zhanbing Cheng Yanbing Xu Lu Yang Jian-Bo Wang Zhenhua Tian Xudong Qu | 2020 | Green Synthesis and Catalysis2020，1，1： | 0 |
| 13 | Norditerpenoids biosynthesized by variediene synthase-associated P450 machinery along with modifications by the host cell Aspergillus oryzae显示文摘The chemical diversity of terpenoids is typically established by terpene synthase-catalyzed cyclization and diversified by post-tailoring modifications.Fungal bifunctional terpene synthase(BFTS)associated P450 enzymes have shown significant catalytic potentials through the development of various new terpenoids with different biological activities.This study discovered the BFTS and its related gene cluster from the plant endophytic fungus Didymosphaeria variabile 17020.Heterologous expression of the BFTS in Saccharomyces cerevisiae resulted in the characterization of a major product diterpene variediene(1),along with two new minor products neovariediene and neoflexibilene.Further heterologous expression of the BFTS and one cytochrome P450 enzyme VndE(CYP6138B1)in Aspergillus oryzae NSAR1 led to the identification of seven norditerpenoids(19 carbons)with a structurally unique 5/5 bicyclic ring system.Interestingly,in vivo experiments suggested that the cyclized terpene variediene(1)was modified by VndE along with the endogenous enzymes from the host cell A.oryzae through serial chemical conversions,followed by multi-site hydroxylation via A.oryzae endogenous enzymes.Our work revealed that the two-enzymes biosynthetic system and host cell machinery could produce structurally unique terpenoids. | Lan Jiang Kangjie Lv Guoliang Zhu Zhi Lin Xue Zhang Cuiping Xing Huanting Yang Weiyan Zhang Zhixin Wang Chengwei Liu Xudong Qu Tom Hsiang Lixin Zhang Xueting Liu | 2022 | Synthetic and Systems Biotechnology2022，7，4： | 0 |
| 14 | 解码氨甲酰多氧草氨酸生物合成:揭示其途径中含有乙酰化循环并偶联串联还原与次序羟化反应显示文摘多氧霉素由可可链霉菌阿苏变种和金产色链霉菌产生,该抗生素结构中含有两个非天然的氨基酸结构单元——氨甲酰多氧草氨酸和聚肟酸。尽管氨甲酰多氧草氨酸结构非常独特,然而其生物合成机理一直悬而未决。 | Jianzhao Qi Dan Wan Hongmin Ma Yuanzhen Liu Rong Gong Xudong Qu Yuhui Sun Zixin Deng 陈文青 | 2017 | 科学新闻2017，19，4： | 0 |
| 15 | ESRC guide vanes of hydraulic turbine for Three Gorges project显示文摘The mechanical properties and internal quality of low carbon martensite Electroslag Remelting Casting (ESRC)stainless steel castings are superior to that of sand casting ones. The key technologies for the equipments and ESRCprocesses have been resolved during the experimental research period of guide vanes of hydraulic turbines for ThreeGorges project. And ESRC guide vanes of hydraulic turbines for Three Gorges project have been produced successfully. | Rui CHEN Xudong LI Junqing WANG Shifang SU Jun YANG Xueliang QU | 2005 | China Foundry2005，2，2： | 0 |
| 16 | The Sterile 20-Like Kinase Tao Controls Tissue Homeostasis by Regulating the Hippo Pathway in Drosophila Adult Midgut显示文摘The proliferation and differentiation of adult stem cells must be tightly controlled in order to maintain resident tissue homeostasis.Dysfunction of stem cells is implicated in many human diseases, including cancer. However, the regulation of stem cell proliferation and differentiation is not fully understood. Here we show that the sterile-like 20 kinase, Tao, controls tissue homeostasis by regulating the Hippo pathway in the Drosophila adult midgut. Depletion of Tao in the progenitors leads to rapid intestinal stem cell(ISC) proliferation and midgut homeostasis loss. Meanwhile, we find that the Janus kinase(JAK)/signal transducer and activator of transcription(STAT)signaling activity and cytokine production are significantly increased, resulting in stimulated ISC proliferation. Furthermore, expression of the Hippo pathway downstream targets, Diap1 and bantam, is dramatically increased in Tao knockdown intestines. Consistently, we show that the Yorkie(Yki) acts downstream of Tao to regulate ISC proliferation. Together, our results provide insights into our understanding of the mechanisms of stem cell proliferation and tissue homeostasis control. | Xudong Huang Lai Shi Jun Cao Fangfei He Renling Li Yan Zhang Shuang Miao Longjin Jin Jia Qu Zhouhua Li Xinhua Lin | 2014 | Journal of Genetics and Genomics2014，41，8： | 0 |
| 17 | Emergent hybrid-dual-graft liver transplantation:a life-saving strategy for a patient with inadequate living donor graft during the COVID-19 pandemic显示文摘Introduction Liver transplantation(LT)benefits patients with decompensated liver cirrhosis,acute liver failure,or hepatocellular carcinoma.The greatest challenge in LT is the insufficient number of donors for the growing number of transplantation candidates.Various surgical strategies have been adopted to overcome the increasing donor-recipient disparity,including sequential/“domino”transplantation,split LT,use of marginal livers,and living donor LT(LDLT).To ensure the safety of donors and recipients,dual-graft LT may be considered(1).Herein,we describe the first successful hybrid-dual-graft LT in an obese patient requiring urgent LT,which involved transplantation of an otherwise-wasted resected liver lobe from a patient with a benign hepatic tumor and an inadequate-size living donor graft from the recipient’s father. | Yongsheng Xiao Xiaowu Huang Yinghong Shi Zheng Wang Xiaoying Wang Yifeng He Ting Wang Jian Sun Kang Song Zhenbin Ding Yinghao Shen Yuanfei Peng Zaozhuo Shen Lei Yu Kai Zhu Hui Li Di Zhou Changhong Miao Yuan Ji Liuxiao Yang Yingyong Hou Xudong Qu Jia Fan Jian Zhou | 2022 | Hepatobiliary Surgery and Nutrition2022，11，5： | 0 |
| 18 | Halogen-doped phosphorescent carbon dots for grayscale patterning显示文摘Flexible organic materials that exhibit dynamic ultralong room temperature phosphorescence(DURTP)via photoactivation have attracted increasing research interest for their fascinating functions of reversibly writing-reading-erasing graphic information in the form of a long afterglow.However,due to the existence of a nonnegligible activation threshold for the initial exposure dose,the display mode of these materials has thus far been limited to binary patterns.By resorting to halogen element doping of carbon dots(CDs)to enhance intersystem crossing and reduce the activation threshold,we were able to produce,for the first time,a transparent,flexible,and fully programmable DURTP composite film with a reliable grayscale display capacity.Examples of promising applications in UV photography and highly confidential steganography were constructed,partially demonstrating the broad future applications of this material as a programmable platform with a high optical information density. | Yanfeng Liu Mahmoud Al-salihi Yong Guo Roman Ziniuk Songtao Cai Luwei Wang Yuan Li Zhigang Yang Dengfeng Peng Kai Xi Zhongfu An Xudong Jia Liwei Liu Wei Yan Junle Qu | 2022 | Light(Science & Applications)2022，11，7： | 0 |
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