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| 1 | TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2显示文摘Endothelial activation plays an essential role in the pathogenesis of sepsis-induced acute lung injury,however,the detailed regulatory mechanisms remain largely unknown.Here,we reported that TRIM47,an E3 ubiquitin ligase of the tripartite motifcontaining protein family,was highly expressed in vascular endothelial cells.TRIM47-deficient mice were effectively resistant to lipopolysaccharide(LPS)-induced acute lung injury and death by attenuating pulmonary inflammation.TRIM47 was upregulated during TNFα-induced endothelial activation in vitro.Knockdown of TRIM47 in endothelial cells inhibited the transcription of multiple pro-inflammatory cytokines,reduced monocyte adhesion and the expression of adhesion molecules,and suppressed the secretion of IL-1βand IL-6 in endothelial cells.By contrast,overexpression of TRIM47 promoted inflammatory response and monocyte adhesion upon TNFαstimulation.In addition,TRIM47 was able to activate the NF-κB and MAPK signaling pathways during endothelial activation.Furthermore,our experiments revealed that TRIM47 resulted in endothelial activation by promoting the K63-linked ubiquitination of TRAF2,a key component of the TNFαsignaling pathway.Taken together,our studies demonstrated that TRIM47 as a novel activator of endothelial cells,promoted LPS-induced pulmonary inflammation and acute lung injury through potentiating the K63-linked ubiquitination of TRAF2,which in turn activates NF-κB and MAPK signaling pathways to trigger an inflammatory response in endothelial cells. | Yisong Qian Ziwei Wang Hongru Lin Tianhua Lei Zhou Zhou Weilu Huang Xuehan Wu Li Zuo Jie Wu Yu Liu Ling-Fang Wang Xiao-Hui Guan Ke-Yu Deng Mingui Fu Hong-Bo Xin | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 2 |
| 2 | RNA-seq reveals hormone-regulated synthesis of non-cellulose polysaccharides associated with fiber strength in a single-chromosomal-fragmentsubstituted upland cotton line显示文摘Cotton fibers are the main raw materials of the textile industry.Exogenous superior fiber genes have been introduced into upland cotton to develop high-yield cultivars with excellent fiber quality.We used a single chromosomal segment on the chromosome A07 substitution line SL7,with high fiber strength,to investigate the molecular mechanism underlying its fiber quality.RNA-seq and KEGG analysis showed that 70 differentially expressed genes were enriched in plant hormone transduction pathways,including auxin,ethylene and abscisic acid,in fibers at 10 days post-anthesis(DPA).Among these,fiberdevelopment related transcription factors MYB and NAC,including Gh_A11G0981(MYB108),Gh_A03G0887(NAC029),and Gh_A08G1691(NAC021),were significantly upregulated in SL7,as were numerous cellulose synthase-like(CSL)genes involved in non-cellulose polysaccharide and cell wall synthesis.The hemicellulose content of SL7 was significantly higher than that of L22,an upland cotton cultivar.These results suggest that key genes in the introgressed chromosomal segment of SL7 regulate the expression of transcription-factor genes via hormone-transduction pathways,thereby inducing the expression of genes involved in secondary wall synthesis and ultimately improving fiber quality.This study has shed light on the molecular mechanism of fiber development and will contribute to the improvement of fiber quality of upland cotton by molecular breeding. | Zhangqiang Song Yu Chen Chuanyun Zhang Jingxia Zhang Xuehan Huo Yang Gao Ao Pan Zhaohai Du Juan Zhou Yanxiu Zhao Zhi Liu Furong Wang Jun Zhang | 2020 | The Crop Journal2020，8，2： | 1 |
| 3 | Transcriptomic analysis of interstockinduced dwarfism in Sweet Persimmon(Diospyros kaki Thunb.)显示文摘Growth monitoring indicated that the height of‘Kanshu’plants with‘Nantong-xiaofangshi’as an interstock was significantly shorter than that of‘Kanshu’plants with no interstock.A transcriptome analysis of the two graft combinations(‘Kanshu’/Diospyros lotus and‘Kanshu’/‘Nantong-xiaofangshi’/Diospyros lotus)was conducted to explore the dwarfing genes related to the use of the‘Nantong-xiaofangshi’interstock.Hormone levels and water conductance were also measured in these two graft combinations.The results indicated that the levels of both IAA and GA were lower in‘Kanshu’that had been grafted onto the‘Nantong-xiaofangshi’interstock than in‘Kanshu’with no interstock;additionally,the water conductance was lower in grafts with interstocks than in grafts without interstocks.The expression of AUX/IAA and auxin-responsive GH3 genes was enhanced in scions grafted on the interstock and was negatively correlated with the IAA content and growth of scions.The expression of GA2ox,DELLA,and SPINDLY genes were also upregulated and associated with a decrease in the level of GA in scions grafted on the interstock.Since one of the GA2ox unigenes was annotated as DkGA2ox1 in Diospyros kaki,but was not functionally validated,a functional analysis was conducted in transgenic tobacco.Overexpression of DkGA2ox1 in transgenic plants resulted in a dwarf phenotype that could be recovered by the exogenous application of GA3.We conclude that the‘Nantong-xiaofangshi’interstock affects the water conductance and expression of genes related to the metabolism and transduction of IAA and GA in the grafted scion and thus regulates phytohormone levels,producing dwarfing. | Yanying Shen Weibing Zhuang Xutong Tu Zhihong Gao Aisheng Xiong Xinyi Yu Xuehan Li Feihong Li Shenchun Qu | 2019 | Horticulture Research2019，6，1： | 1 |
| 4 | Regional Economic Development Trend Prediction Method Based on Digital Twins and Time Series Network显示文摘At present,the interpretation of regional economic development(RED)has changed from a simple evaluation of economic growth to a focus on economic growth and the optimization of economic structure,the improvement of economic relations,and the change of institutional innovation.This article uses the RED trend as the research object and constructs the RED index to conduct the theoretical analysis.Then this paper uses the attention mechanism based on digital twins and the time series network model to verify the actual data.Finally,the regional economy is predicted according to the theoretical model.The specific research work mainly includes the following aspects:1)This paper introduced the development status of research on time series networks and economic forecasting at home and abroad.2)This paper introduces the basic principles and structures of long and short-term memory(LSTM)and convolutional neural network(CNN),constructs an improved CNN-LSTM model combined with the attention mechanism,and then constructs a regional economic prediction index system.3)The best parameters of the model are selected through experiments,and the trained model is used for simulation experiment prediction.The results show that the CNN-LSTM model based on the attentionmechanism proposed in this paper has high accuracy in predicting regional economies. | Runguo Xu Xuehan Yu Xiaoxue Zhao | 2023 | Computers, Materials & Continua2023，76，8： | 0 |
| 5 | Stem Cell-Based Hair Cell Regeneration and Therapy in the Inner Ear显示文摘Hearing loss has become increasingly prevalent and causes considerable disability,thus gravely burdening the global economy.Irreversible loss of hair cells is a main cause of sensorineural hearing loss,and currently,the only relatively effective clinical treatments are limited to digital hearing equipment like cochlear implants and hearing aids,but these are of limited benefit in patients.It is therefore urgent to understand the mechanisms of damage repair in order to develop new neuroprotective strategies.At present,how to promote the regeneration of functional hair cells is a key scientific question in the field of hearing research.Multi-ple signaling pathways and transcriptional factors trigger the activation of hair cell progenitors and ensure the maturation of newborn hair cells,and in this article,we first review the principal mechanisms underlying hair cell reproduction.We then further discuss therapeutic strategies involving the co-regulation of multiple signaling pathways in order to induce effective functional hair cell regeneration after degeneration,and we summarize current achievements in hair cell regeneration.Lastly,we discuss potential future approaches,such as small molecule drugs and gene therapy,which might be applied for regenerating functional hair cells in the clinic. | Jieyu Qi Wenjuan Huang Yicheng Lu Xuehan Yang Yinyi Zhou Tian Chen Xiaohan Wang Yafeng Yu Jia-Qiang Sun Renjie Chai | 2024 | Neuroscience Bulletin2024，40，1： | 0 |
| 6 | The Establishment of Immunochemistry Test Based on a Synthetic Peptide Antibody for the Detection of a Porcine Circovirus-Like Virus P1显示文摘Recently, a novel porcine circovirus-like virus P1 with a circular DNA genome of 0.648 kb was identified. P1 antigen was detected both in vitro and in vivo by synthetic peptide-derived polyclonal antibody-based immunochemistry. The designed peptides were synthesized by solid-phase technique, purified by high performance liquid chromatography, coupled to Keyhole limpet hemocyanin, and injected into rabbits to prepare polyclonal antibody. The emergence of positive cells revealed that synthetic peptide could elicit antibodies against P1 and viral protein could be synthesized. The polyclonal peptide antibodies described here was successfully applied to immunochemical staining and proved helpful in diagnosing P1. | Libin WEN Fengzhi WANG Kongwang HE Yanxiu NI Xuehan ZHANG Rongli GUO Bin LI Xiaomin WANG Zhengyu YU Aihua MAO Junming ZHOU Lixin LU Jianping XIE | 2014 | Agricultural Science & Technology2014，15，2： | 0 |
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