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| 1 | Aptamer-conjugated upconversion nanoprobes assisted by magnetic separation for effective isolation and sensitive detection of circulating tumor cells显示文摘 | Shuai Fang Chao Wang Jian Xiang Liang Cheng Xuejiao Song Ligeng Xu Rui Peng Zhuang Liu | 2014 | Nano Research2014，7，9： | 10 |
| 2 | A natural variation in Ribonuclease H-like gene underlies Rht8 to confer“Green Revolution”trait in wheat显示文摘Dear Editor,Introduction of gibberellin(GA)-insensitive Reduced height(Rht)genes,Rht-B1b and Rht-D1b,has resulted in the“Green Revolution”in modern wheat cultivars(Triticum aestivum)that has skyrocketed wheat grain yields worldwide since the 1960s(Peng et al.,1999;Velde et al.,2021).However,Rht-B1b/D1b also reduce coleoptiles,which is undesired in dryland regions where deep planting is essential for seedling establishment(Rebetzke et al.,1999,Rebetzke et al.,2001;Ellis et al.,2004). | Lingling Chai Mingming Xin Chaoqun Dong Zhaoyan Chen Huijie Zhai Junhong Zhuang Xuejiao Cheng Naijiao Wang Jia Geng Xiaobo Wang Ruolin Bian Yingyin Yao Weilong Guo Zhaorong Hu Huiru Peng Guihua Bai Qixin Sun Zhenqi Su Jie Liu Zhongfu Ni | 2022 | Molecular Plant2022，15，3： | 8 |
| 3 | Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury显示文摘Spinal cord injury(SCI)involves diverse injury responses in different cell types in a temporally and spatially specific manner.Here,using single-cell transcriptomic analyses combined with classic anatomical,behavioral,electrophysiological analyses,we report,with single-cell resolution,temporal molecular and cellular changes in crush-injured adult mouse spinal cord.Data revealed pathological changes of 12 different major cell types,three of which infiltrated into the spinal cord at distinct times post-injury.We discovered novel microglia and astrocyte subtypes in the uninjured spinal cord,and their dynamic conversions into additional stage-specific subtypes/states.Most dynamic changes occur at 3-days post-injury and by day-14 the second wave of microglial activation emerged,accompanied with changes in various cell types including neurons,indicative of the second round of attacks.By day-38,major cell types are still substantially deviated from uninjured states,demonstrating prolonged alterations.This study provides a comprehensive mapping of cellular/molecular pathological changes along the temporal axis after SCI,which may facilitate the development of novel therapeutic strategies,including those targeting microglia. | Chen Li Zhourui Wu Liqiang Zhou Jingliang Shao Xiao Hu Wei Xu Yilong Ren Xingfei Zhu Weihong Ge Kunshan Zhang Jiping Liu Runzhi Huang Jing Yu Dandan Luo Xuejiao Yang Wenmin Zhu Rongrong Zhu Changhong Zheng Yi Eve Sun Liming Cheng | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 4 |
| 4 | Ectopic expression of VRT-A2 underlies the origin of Triticum polonicum and Triticum petropavlovskyi with long outer glumes and grains显示文摘Polish wheat (Triticum polonicum) is a unique tetraploid wheat species characterized by an elongated outer glume. The genetic control of the long-glume trait by a single semi-dominant locus, P1 (from Polish wheat), was established more than 100 years ago, but the underlying causal gene and molecular nature remain elusive. Here, we report the isolation of VRT-A2, encoding an SVP-clade MADS-box transcription factor, as the P1 candidate gene. Genetic evidence suggests that in T. polonicum, a naturally occurring sequence rearrangement in the intron-1 region of VRT-A2 leads to ectopic expression of VRT-A2 in floral organs where the long-glume phenotype appears. Interestingly, we found that the intron-1 region is a key ON/OFF molecular switch for VRT-A2 expression, not only because it recruits transcriptional repressors, but also because it confers intron-mediated transcriptional enhancement. Genotypic analyses using wheat accessions indicated that the P1 locus is likely derived from a single natural mutation in tetraploid wheat, which was subsequently inherited by hexaploid T. petropavlovskyi. Taken together, our findings highlight the promoter-proximal intron variation as a molecular basis for phenotypic differentiation, and thus species formation in Triticum plants. | Jing Liu Zhaoyan Chen Zhihui Wang Zhaoheng Zhang Xiaoming Xie Zihao Wang Lingling Chai Long Song Xuejiao Cheng Man Feng Xiaobo Wang Yanhong Liu Zhaorong Hu Jiewen Xing Zhenqi Su Huiru Peng Mingming Xin Yingyin Yao Weilong Guo Qixin Sun Jie Liu Zhongfu Ni | 2021 | Molecular Plant2021，14，9： | 4 |
| 5 | Clinical Predictors of Functional Cure in Children 1–6 Years-old with Chronic Hepatitis B显示文摘Background and Aims:Hepatitis B surface antigen(HBsAg)clearance is significantly more common in children with chronic hepatitis B(CHB)than in adults;however,the possible influencing factors related to HBsAg loss have yet to be found.This study aimed to explore the efficacy of long-term interferon(IFN)αtherapy in treating children with CHB and analyzed the factors influencing functional cure after treatment.Methods:A total of 236 children aged 1–6 years and diagnosed with CHB via liver biopsy were included in the study,all receiving IFNαtreatment(IFNα-2b monotherapy,IFNα-2b followed by lamivudine[LAM]or IFNα-2b combined with LAM)and followed up for 144 weeks.A comprehensive analysis was conducted on clinical data,including biochemical items,serum markers of hepatitis B virus(HBV)and immunological indexes,and logistic regression analysis was used to screen the influencing factors related to HBsAg loss.Results:The cumulative loss rates of HBsAg were 79.5%,62.1%and 42.1%at 144 weeks after the start of treatment in the 1–3 years-old group,3–5 years-old group and 5–7 years-old group,respectively(p<0.05).IFNα-2b combined with LAM treatment displayed the highest HBsAg loss rates compared with monotherapy and sequential treatment(p=0.011).Younger baseline age and lower HBsAg levels were independent factors for the prediction of HBsAg loss(p<0.05).The baseline PreS1 and hepatitis B core antibody levels in the HBsAg loss group were lower than those in the HBsAg non-loss group.In addition,the PreS1 level was positively corelated with the level of HBsAg,HBV DNA and liver inflammation.Conclusions:Long-term treatment with IFNαwas effective in achieving HBsAg loss in CHB children aged 1–6 years-old.Age less than 3 years-old and lower HBsAg levels are independent predictors of functional cure in children with CHB. | Jing Pan Haiyan Wang Tiantian Yao Xuejiao Liao Hao Cheng Suthat Liangpunsakul Yan Wang Min Zhang Zheng Zhang | 2022 | Journal of Clinical and Translational Hepatology2022，10，3： | 3 |
| 6 | BYSL contributes to tumor growth by cooperating with the mTORC2 complex in gliomas显示文摘Objective:BYSL,which encodes the Bystin protein in humans,is upregulated in reactive astrocytes following brain damage and/or inflammation.We aimed to determine the role and mechanism of BYSL in glioma cell growth and survival.Methods:BYSL expression in glioma tissues was measured by quantitative real-time PCR,Western blot,and immunohistochemistry.In vitro assays were performed to assess the role of BYSL in cell proliferation and apoptosis.Protein interactions and co-localization were determined by co-immunoprecipitation and double immunofluorescence.The expression and activity of the AKT/m TOR signaling molecules were determined by Western blot analysis,and the role of BYSL in glioma growth was confirmed in an orthotopic xenograft model.Results:The BYSL m RNA and protein levels were elevated in glioma tissues.Silencing BYSL inhibited glioma cell proliferation,impeded cell cycle progression,and induced apoptosis,whereas overexpressing BYSL protein led to the opposite effects.We identified a complex consisting of BYSL,RIOK2,and m TOR,and observed co-localization and positive correlations between BYSL and RIOK2 in glioma cells and tissues.Overexpressing BYSL or RIOK2 increased the expression and activity of AKT/m TOR signaling molecules,whereas downregulation of BYSL or RIOK2 decreased the activity of AKT/m TOR signaling molecules.Silencing BYSL or RIOK2 decreased the growth of the tumors and prolonged the lifespan of the animals in an orthotopic xenograft model.Conclusions:High expression of BYSL in gliomas promoted tumor cell growth and survival both in vitro and in vivo.These effects could be attributed to the association of BYSL with RIOK2 and m TOR,and the subsequent activation of AKT signaling. | Shangfeng Gao Zhuang Sha Junbo Zhou Yihao Wu Yunnong Song Cheng Li Xuejiao Liu Tong Zhang Rutong Yu | 2021 | Cancer Biology & Medicine2021，18，1： | 3 |
| 7 | Generating Giant Membrane Vesicles from Live Cells with Preserved Cellular Properties显示文摘Biomimetic giant membrane vesicles,with size and lipid compositions comparable to cells,have been recognized as an attractive experimental alternative to living systems.Due to the similarity of their membrane structure to that of body cells,cell-derived giant plasma membrane vesicles have been used as a membrane model for studying lipid/protein behavior of plasma membranes.However,further application of biomimetic giant membrane vesicles has been hampered by the side-efects of chemical vesiculants and the utilization of osmotic bufer.We herein develop a facile strategy to derive giant membrane vesicles(GMVs)from mammalian cells in biofriendly medium with high yields.Tese GMVs preserve membrane properties and adaptability for surface modifcation and encapsulation of exogenous molecules,which would facilitate their potential biological applications.Moreover,by loading GMVs with therapeutic drugs,GMVs could be employed for drug transport to tumor cells,which represents another step forward in the biomedical application of giant membrane vesicles.Tis study highlights biocompatible GMVs with biomimicking membrane surface properties and adaptability as an ideal platform for drug delivery strategies with potential clinical applications. | Qiaoling Liu Cheng Bi Jiangling Li Xuejiao Liu Ruizi Peng Cheng Jin Yang Sun Yifan Lyu Hui Liu Huijing Wang Can Luo Weihong Tan | 2019 | Research2019，，1： | 2 |
| 8 | Ametryn degradation by aqueous chlorine: Kinetics and reaction influences显示文摘 | Bin Xu Nai-yun Gao Hefa Cheng Chen-yan Hu Sheng-ji Xia Xiao-feng Sun Xuejiao Wang Shaogui Yang | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 9 | Structural tailoring of covalent organic frameworks with steric effects显示文摘Covalent organic frameworks(COFs)provide a unique platform with tunable structures allowing precise control of pore sizes,shapes and functions.The key to synthesizing COFs with desired structures is to precisely control the conformation and geometry of building blocks as well as the growth direction of COFs.To achieve this,steric effects are noteworthy that may have a significant impact on the assembly of COFs.Specifically,the introduction of sterically demanding substituents or bulky groups into monomers of COFs will lead to intramolecular conformational changes and intermolecular repulsions,which induce structural changes in COFs,including changes in torsion angles,interlayer distances,stacking modes and topologies of 2D COFs,and changes in spatial nodes,interpenetration and topologies of 3D COFs.This review will help to understand the impacts of steric effects on the structures of COFs and to take them into extensive consideration in the design and synthesis of COFs with novel functionalities and structural attributes. | Guiqing Lin Arindam Mal Xuejiao Wang Xu Zhou Bo Gui Cheng Wang | 2023 | Science China Chemistry2023，66，11： | 0 |
| 10 | Study on the Optimization of Extraction Technology of Rose laevigate Michx. Polysaccharides and Its in vitro Antioxidant Activity显示文摘[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides. | Guochen HU Han LIU Qianqian TANG Li CHENG Chenzhong JIN Yunyun ZHOU Xuejiao ZHANG Guiying LIU | 2021 | Agricultural Biotechnology2021，10，6： | 0 |
| 11 | Highly delocalized endohedral metal in Gd@C2v(9)-C82 metallofullerenes co-crystallized with α-Se显示文摘 | Cheng Li Xuejiao J. Gao Huanli Yao Huan Huang Rongli Cui Xihong Guo Lele Zhang Bing Liu Binggang Xu Weiqun Shi Jinquan Dong Lai Feng Xingfa Gao Baoyun Sun | 2018 | Nano Research2018，11，4： | 0 |
| 12 | TaACTIN7-D regulates plant height and grain shape in bread wheat显示文摘Exploitation of new gene resources and genetic networks contributing to the control of crop yield-related traits,such as plant height,grain size,and shape,may enable us to breed modern high-yielding wheat varieties through molecular methods.In this study,via ethylmethanesulfonate mutagenesis,we identify a wheat mutant plant,mu-597,that shows semi-dwarf plant architecture and round grain shape.Through bulked segregant RNA-seq and map-based cloning,the causal gene for the semi-dwarf phenotype of mu-597 is located.We find that a single-base mutation in the coding region of TaACTIN7-D(TaACT7-D),leading to a Gly-to-Ser(G65S)amino acid mutation at the 65th residue of the deduced TaACT7-D protein,can explain the semi-dwarfism and round grain shape of mu-597.Further evidence shows that the G65S mutation in TaACT7-D hinders the polymerization of actin from monomeric(G-actin)to filamentous(F-actin)status while attenuates wheat responses to multiple phytohormones,including brassinosteroids,auxin,and gibberellin.Together,these findings not only define a new semi-dwarfing gene resource that can be potentially used to design plant height and grain shape of bread wheat but also establish a direct link between actin structure modulation and phytohormone signal transduction. | Xiongtao Li Beilu Cao Dejie Du Long Song Lulu Tian Xiaoming Xie Zhaoyan Chen Yanpeng Ding Xuejiao Cheng Yingyin Yao Weilong Guo Zhenqi Su Qixin Sun Zhongfu Ni Lingling Chai Jie Liu | 2023 | Journal of Genetics and Genomics2023，50，11： | 0 |
| 13 | Role of Ras-related Nuclear Protein/Polypyrimidine Tract Binding Protein in Facilitating the Replication of Hepatitis C Virus显示文摘Background and Aims:Ras-related nuclear(RAN)protein is a small GTP-binding protein that is indispensable for the translocation of RNA and proteins through the nuclear pore complex.Recent studies have indicated that RAN plays an important role in virus infection.However,the role of RAN in hepatitis C virus(HCV)infection is unclear.The objective of this study was to investigate the role and underlying mechanisms of RAN in HCV infection.Methods:Huh7.5.1 cells were infected with the JC1-Luc virus for 24 h and then were incubated with complete medium for an additional 48 h.HCV infection and RAN expression were determined using luciferase assay,quantitative reverse transcription-PCR and western blotting.Small interfering RNA was used to silence RAN.Western blotting and immunofluorescence were used to evaluate the cytoplasmic translocation of polypyrimidine tract-binding(PTB),and coimmunoprecipitation was used to examine the interaction between RAN and PTB.Results:HCV infection significantly induced RAN expression and cytoplasmic redistribution of PTB.Knockdown of RAN dramatically inhibited HCV infection and the cytoplasmic accumulation of PTB.Colocalization of RAN and PTB was determined by immunofluorescence,and a direct interaction of RAN with PTB was demonstrated by coimmunoprecipitation.Conclusions:PTB in the host cytoplasm is directly associated with HCV replication.These findings demonstrate that the involvement of RAN in HCV infection is mediated by influencing the cytoplasmic translocation of PTB. | Jihua Xue Jun Cheng Xuejiao Ma Yixian Shi Huafa Yin Yufeng Gao Jiabin Li | 2021 | Journal of Clinical and Translational Hepatology2021，9，4： | 0 |
| 14 | Tuning Energy Transfer in Metal-OrganicFrameworks for Fluorescence Turn-on Sensing of Hg(Ⅱ)Ions显示文摘Tuning F?rster resonance energy transfer(FRET)in metal-organic frameworks for fluorescence turn on sensing is rarely reported.Herein,we designed and synthesized several FRET-based metal-organic frameworks(MOFs)with different donor/acceptor ratios.Through the oxidation of the acceptor,the FRET process in MOFs can be efficiently modulated so as to turn-on the fluorescence of the framework in a controlled manner.Interestingly,the MOF with an optimized donor/acceptor ratio of 7.0 was used as an efficient fluorescence turn on sensor for Hg(Ⅱ)ions with good sensitivity and selectivity.We believe our present study will not only give a useful method to construct fluorescence turn-on sensors but also trigger the construction of novel FRET-based MOFs for the fluo-rescence turn-on sensing of target analytes in the future. | Xuejiao Wang Arindam Mal Bo Gui Cheng Wang | 2023 | Chinese Journal of Chemistry2023，41，9： | 0 |
| 15 | Correction to:Temporal and spatial cellular and molecular pathological alterations with single-cell resolution in the adult spinal cord after injury显示文摘 | Chen Li Zhourui Wu Liqiang Zhou Jingliang Shao Xiao Hu Wei Xu Yilong Ren Xingfei Zhu Weihong Ge Kunshan Zhang Jiping Liu Runzhi Huang Jing Yu Dandan Luo Xuejiao Yang Wenmin Zhu Rongrong Zhu Changhong Zheng Yi Eve Sun Liming Cheng | 2022 | Signal Transduction and Targeted Therapy2022，7，6： | 0 |
| 16 | Effect of Dandelion(Taraxacum mongolicum Hand.-Mazz.)Intercropping with Different Plant Spacing on Blight and Growth of Pepper(Capsicum annuum L.)显示文摘Intercropping of crops that can secrete bacteriostatic active substances can not only inhibit the occurrence of disease but also have an important effect on plant growth.However,the effects of dandelion intercropping on pepper blight control and pepper growth remain unclear.In this study,the control effect of dandelion on pepper blight was studied by inoculating the pepper leaves with Phytophthora infestans,and it also discusses the correlation of the occurrence of pepper epidemic disease with the pepper canopy environment,soil environment,pepper photo-synthesis,and yield index.The results showed that best plant distance for dandelion intercropping was 20 cm(P20),and the control effect reached 43.31%.As compared to the CK,SOD enzyme,POD enzyme,and PAL enzyme were significantly up-regulated during the growth of pepper;chlorophyll content in pepper leaves was significantly increased;photosynthetic characteristics were significantly increased;stem diameter and yield of crop pepper were effectively improved;and the quality of the pepper product was better,but intercropping dandelion resulted in a significant decrease of nutrients in the soil environment of pepper,so a reasonable intercropping distance was needed.The correlation analysis shows that the incidence of pepper blight(A)was significantly positively correlated with soil temperature(Q),intercellular carbon dioxide(L),and canopy air temperature(O).The incidence of capsicum blight(A)was significantly negatively correlated with chlorophyll content(F),net photo-synthetic rate(K),stomatal conductance(M),ww rate(N),soil sucrase activity(W),vitamin C(AB),and leaf PAL enzyme(J).Finally,it was deduced that intercropping dandelion could effectively control the occurrence of pepper blight while also demonstrating a complex interaction with the pepper growing environment. | Peixin Li Hanbing Liu Yingtong Chen Xin Zhang Ning Cao Ying Sun Meimei Jia Mengran Wu Xuejiao Tong Xinmei Jiang Xihong Yu Yao Cheng | 2023 | Phyton-International Journal of Experimental Botany2023，92，8： | 0 |
| 17 | Genomic,transcriptomic,and epigenomic analysis of a medicinal snake,Bungarus multicinctus,to provides insights into the origin of Elapidae neurotoxins显示文摘The many-banded krait,Bungarus multicinctus,has been recorded as the animal resource of JinQianBaiHuaShe in the Chinese Pharmacopoeia.Characterization of its venoms classified chief phyla of modern animal neurotoxins.However,the evolutionary origin and diversification of its neurotoxins as well as biosynthesis of its active compounds remain largely unknown due to the lack of its high-quality genome.Here,we present the 1.58 Gbp genome of B.multicinctus assembled into 18 chromosomes with contig/scaffold N50 of 7.53 Mbp/149.8 Mbp.Major bungarotoxin-coding genes were clustered within genome by family and found to be associated with ancient local duplications.The truncation of glycosylphosphatidylinositol anchor in the 3'-terminal of a LY6E paralog released modern three-finger toxins(3FTxs)from membrane tethering before the Colubroidea divergence.Subsequent expansion and mutations diversified and recruited these 3FTxs.After the cobra/krait divergence,the modern unit-B ofβ-bungarotoxin emerged with an extra cysteine residue.A subsequent point substitution in unit-A enabled theβ-bungarotoxin covalent linkage.The B.multicinctus gene expression,chromatin topological organization,and histone modification characteristics were featured by transcriptome,proteome,chromatin conformation capture sequencing,and ChIP-seq.The results highlighted that venom production was under a sophisticated regulation.Our findings provide new insights into snake neurotoxin research,meanwhile will facilitate antivenom development,toxin-driven drug discovery and the quality control of JinQianBaiHuaShe. | Jiang Xu Shuai Guo Xianmei Yin Mingqian Li He Su Xuejiao Liao Qiushi Li Liang Le Shiyu Chen Baosheng Liao Haoyu Hu Juan Lei Yingjie Zhu Xiaohui Qiu Lu Luo Jun Chen Ruiyang Cheng Zhenzhan Chang Han Zhang Nicholas Chieh Wu Yiming Guo Dianyun Hou Jin Pei Jihai Gao Yan Hua Zhihai Huang Shilin Chen | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 0 |
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