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| 1 | The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening显示文摘Ethylene plays a pivotal role in climacteric fruit ripening;whereas 1-MCP,a non-toxic antagonist of ethylene,prevents ethylene-dependent responses and fruit ripening.In this study,a short-term treatment(1 h)with 400 nL L^(−1)1-MCP delayed the ripening of harvested papaya.However,long-term application of 1-MCP(400 nL L^(−1),16 h)resulted in abnormal fruit ripening,with the fruits exhibiting normal yellowing without softening,significantly higher cellulose and lignin contents,and intact cell walls(CW).Furthermore,we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1,an EIN3-binding F-box-1 gene.A protein interaction analysis using yeast two-hybrid,BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpEIL1 proteins.The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters.Subcellular localization showed that these proteins were localized in the nucleus.Additionally,the expression levels of CpMADS1/3,CpEIL1,and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment.Therefore,we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction,thereby resulting in fruit softening disorders. | Xiaochun Ding Xiaoyang Zhu Lanlan Ye Shuangling Xiao Zhenxian Wu Weixin Chen Xueping Li | 2019 | Horticulture Research2019，6，1： | 7 |
| 2 | Ability to detect weak effective seismic signals by utilizing chaotic vibrator system显示文摘Taking the advantage of CVS (Chaotic Vibrator System) sensitivity of large-scale periodic phase-state response to quasi-periodic or periodic signals,a series of numerical experiments were made to understand the ability of CVS to detect weak effective seismic signals in the common-shot seismic record distorted by strong stochastic noise. The re-sults demonstrate that the large-scale periodic phase-states of CVS are correlated with the signal composition of the quasi-periodic wavelet sequence constructing from horizontal moveout of seismic events,noise strength and the noise distortion de-gree to signal. For the same kind of events,the higher the noise distortion degree is,the lower the detectable SNR can be reached by CVS. For seismic data with the same noise distortion degree,the closer the scanning seismic velocity (the trial moveout ve-locity) approaches to the accurate velocity,the higher the detectable SNR can be reached by CVS. More-over,the truncating scanning velocities form an asymmetric belt,which indirectly makes CVS achieve a large-scale periodic phase-state and then the ratio of wavelet distortion coefficients in events can be a biggish variable scope. | LI Yue YANG Baojun YUAN Ye ZHAO Xueping LIN Hongbo | 2006 | Chinese Science Bulletin2006，51，24： | 6 |
| 3 | Rapid Detection of Wheat Blast Pathogen Magnaporthe oryzae Triticum Pathotype Using Genome-Specific Primers and Cas12a-mediated Technology显示文摘Wheat blast,caused by the fungus Magnaporthe oryzae Triticum(MoT)pathotype,is a devastating disease persistent in South America and Bangladesh.Since MoT generally fails to cause visual symptoms in wheat until the heading stage when the infection would have advanced,disease control by fungicide application solely based on the detection of visual symptoms is ineffective.To develop an accurate and sensitive method to detect MoT at the seedling and vegetative stages for disease control,we sequenced the genomes of two MoT isolates from Brazil and identified two DNA fragments,MoT-6098 and MoT-6099,that are present in the MoT genome but not in the genome of the rice-infecting Magnaporthe oryzae Oryzae(MoO)pathotype.Using polymerase chain reaction(PCR),we confirmed the specificity of the two markers in 53 MoT and MoO isolates from South America and Bangladesh.To test the efficiency of the two markers,we first established a loop-mediated isothermal amplification(LAMP)method to detect MoT at isothermal conditions,without the use of a PCR machine.Following this,we used the Cas12a protein and guide RNAs(gRNAs)to target the MoT-6098 and MoT-6099 sequences.The activated Cas12a showed indiscriminate single-stranded deoxyribonuclease(ssDNase)activity.We then combined targetdependent Cas12a ssDNase activation with recombinase polymerase amplification(RPA)and nucleic acid lateral flow immunoassay(NALFIA)to develop a method that accurately,sensitively,and cost-effectively detects MoT-specific DNA sequences in infected wheat plants.This novel technique can be easily adapted for the rapid detection of wheat blast and other important plant diseases in the field. | Houxiang Kang Ye Peng Kangyu Hua Yufei Deng Maria Bellizzi Dipali Rani Gupta Nur Uddin Mahmud Alfredo S.Urashima Sanjoy Kumar Paul Gary Peterson Yilin Zhou Xueping Zhou Md Tofazzal Islam Guo-Liang Wang | 2021 | Engineering2021，7，9： | 4 |
| 4 | Biological roles and potential clinical values of circular RNAs in gastrointestinal malignancies显示文摘Circular RNAs(circ RNAs),a class of endogenous RNA molecules,are produced by alternative splicing of precursor RNA and are covalently linked at the 5′and 3′ends.Recent studies have revealed that dysregulated circ RNAs are closely related to the occurrence and progression of gastrointestinal malignancies.Accumulating evidence indicates that circ RNAs,including circ PVT1,circ LARP4,circ-SFMBT2,cir-ITCH,circ RNA_100782,circ_100395,circ-DONSON,hsa_circ_0001368,circ NRIP1,circ FAT1(e2),circ CCDC66,circ SMARCA5,circ-ZNF652,and circ_0030235 play important roles in the proliferation,differentiation,invasion,and metastasis of cancer cells through a variety of mechanisms,such as acting as micro RNA sponges,interacting with RNA-binding proteins,regulating gene transcription and alternative splicing,and being translated into proteins.With the characteristics of high abundance,high stability,extensive functions,and certain tissue-,time-and diseasespecific expressions,circ RNAs are expected to provide novel perspectives for the diagnoses and treatments of gastrointestinal malignancies. | Xueping Tao Yongfu Shao Jianing Yan Liyang Yang Qihua Ye Qingling Wang Rongdan Lu Junming Guo | 2021 | Cancer Biology & Medicine2021，18，2： | 2 |
| 5 | Pathway-pathway network-based study of the therapeutic mechanisms by which salvianolic acid B regulates cardiovascular diseases显示文摘Investigation of the potential therapeutic mechanisms of drug candidates is an essential step in the process of new drug discovery.With the rapid development of systems biology,recent network analyses of proteins,drugs,and diseases have enabled great progress in delineating the molecule mechanisms of drug candidates.However,most analyses perform a direct association between gene/protein and disease levels without considering the intermediate biological pathways regulated by the drugs.Given that a protein performs its biological roles through pathways,we propose using a novel pathway-pathway network analysis to investigate the potential therapeutic functions of the drug candidates.Many studies have demonstrated that salvianolic acid B(SalB) of Salvia miltiorrhiza is an effective therapy for cardiovascular diseases(CVD).Using molecular docking methods to identify direct interacting targets of Sal B,we collected all Sal B-regulated proteins with supporting experimental evidence in PubMed abstracts.FDA-approved CVD drugs and their corresponding targets were also collected.From a traditional drug-protein network analysis,we found that Sal B could affect ACE and REN of the renin-angiotensin-aldosterone system to relax vessels and alleviate hypertension.Subsequent pathway-pathway network analysis was attempted to study the mechanisms of Sal B in treating CVD,and demonstrated that Sal B regulates immunity/inflammation,apoptosis,ion transport and basic metabolism processes in the treatment of CVD.Regulating the immune/inflammation process may be the major mechanism of Sal B.We believe that pathwaypathway network analysis is a novel method for studying the therapeutic mechanisms of herbal ingredients. | YE Li HE Yuan YE Hao LIU XuePing YANG LinLin CAO ZhiWei TANG KaiLin | 2012 | Chinese Science Bulletin2012，57，14： | 1 |
| 6 | The isolation and characterization of polysaccharides from longan pulp显示文摘 | Cuixian Yang Ning He Xueping Ling Meiling Ye Chenxiao Zhang Wenyao Shao Chuanyi Yao Zhiyuan Wang Qingbiao Li | 2008 | Separation and Purification Technology2008，，1： | 1 |
| 7 | Variation Laws and Influencing Factors of pH in Fishery Waters in Quzhou City显示文摘[Objectives] This study was conducted to strengthen the understanding of the variation laws of pH in fishery waters. [Methods]Based on the investigation data of pH, dissolved oxygen(Do), water temperature(T) and chlorophyll a(Chl. a) of the fishery waters in Quzhou City from 2017 to 2020, the seasonal variation characteristics and influencing factors of pH in the fishery waters were analyzed and discussed. [Results] The pH of the fishery waters in Quzhou City was in the range of 6.40-11.3, and was characterized by the highest seasonal variation in spring and the lowest in winter. The correlation analysis showed that water temperature and pH were significantly positively correlated in spring and summer(P<0.05), and the photosynthesis of phytoplankton was the main reason that affected the pH of water bodies in spring and summer. There was a negative correlation between water temperature and pH in winter, and the pH in the natural waters had a significant negative correlation with water temperature(P<0.05). The balance of carbonate system in the water bodies was the main reason that affected the pH of water in winter. The level of water eutrophication(Chl. a) affected the pH of fishery waters to a certain extent. When Chl. a<10 μg/L, there was a significant positive correlation between the pH of fishery waters and Chl. a(P<0.05). Various environmental factors had different effects on the pH of different types of fishery waters. The stepwise regression analysis showed that the pH of natural waters was mainly affected by Chl. a, and the pH of aquaculture waters was mainly affected by water temperature and dissolved oxygen. [Conclusions]This study provides certain theoretical support for actual production management. | Qinxuan SHI Xueping YE Dongren ZHOU Sheng GAO Qifang WU Feng LIN Ting YE | 2021 | Agricultural Biotechnology2021，10，1： | 1 |
| 8 | Identification and Biological Characteristics of Edwardsiella tarda Isolated from Pelteobagrus fulvidraco显示文摘Over the past five years,an epidemic disease broke out in Pelteobagrus fulvidraco ponds of Zhejiang Province. The diseased fish mainly exhibits head splitting and surface bleeding; dissection results reveal hepatonephromegaly and abdominal bleeding. A bacterial strain HSY201301 was isolated from the liver tissue of P. fulvidraco with typical symptoms. Artificial infection experiment confirmed that the isolated strain had a strong virulence to healthy P. fulvidraco,leading to similar symptoms to naturally infected P. fulvidraco. The isolated strain was identified as an Edwardsiella tarda strain according to conventional morphological,physiological,biochemical characteristics and 16 S rRNA gene sequence. Results of drug susceptibility test indicated that the isolated strain was sensitive to ciprofloxacin,doxitard,penicillin,doxycycline,and rocephin. This study laid solid foundation for effective prevention and control of E. tarda. | Dongren ZHOU Xueping YE Yizhi LUO Weida SHI Xiaoying HANG | 2014 | Agricultural Biotechnology2014，3，5： | 1 |
| 9 | BGB-A445,a novel non-ligand-blocking agonistic anti-OX40 antibody,exhibits superior immune activation and antitumor effects in preclinical models显示文摘t OX40 is a costimulatory receptor that is expressed primarily on activated CD4+,CD8+,and regulatory T cells.The ligation of OX40 to its sole ligand OX40L potentiates T cell expansion,differentiation,and activation and also promotes dendritic cells to mature to enhance their cytokine production.Therefore,the use of agonistic anti-Ox40 antibodies for cancer immunotherapy has gained great interest.However,most of the agonistic anti-OX40 antibodies in the clinic are OX40L-competitive and show limited efficacy.Here,we discovered that BGB-A445,a non-ligand-competitive agonistic anti-OX40 antibody currently under clinical investigation,induced optimal T cell activation without impairing dendritic cell function.In addition,BGB-A445 dose-dependently and significantly depleted regulatory T cells in vitro and in vivo via antibody-dependent cellular cytotoxicity.In the MC38 syngeneic model established in humanized OX40 knock-in mice,BGB-A445 demonstrated robust and dose-dependent antitumor efficacy,whereas the ligand-competitive anti-Ox40 antibody showed antitumor efficacy characterized by a hook effect.Furthermore,BGB-A445 demonstrated a strong combination antitumor effect with an anti-PD-1 antibody.Taken together,our findings show that BGB-A445,which does not block OX40-OX40L interaction in contrast to clinical-stage anti-OX40 antibodies,shows superior immune-stimulating effects and antitumor efficacy and thus warrants further clinical investigation. | Beibei Jiang Tong Zhang Minjuan Deng Wei Jin Yuan Hong Xiaotong Chen Xin Chen Jing Wang Hongjia Hou Yajuan Gao Wenfeng Gong Xing Wang Haiying Li Xiaosui Zhou Yingcai Feng Bo Zhang Bin Jiang Xueping Lu Lijie Zhang Yang Li Weiwei Song Hanzi Sun Zuobai Wang Xiaomin Song Zhirong Shen Xuesong Liu Kang Li Lai Wangl Ye Liu | 2023 | Frontiers of Medicine2023，17，6： | 0 |
| 10 | Tomato leaf curl New Delhi virus:an emerging plant begomovirus threatening cucurbit production显示文摘Tomato leaf curl New Delhi virus(ToLCNDV),a bipartite begomovirus,was first reported to infect tomato and has recently spread rapidly as an emerging disease to Cucurbitaceae crops.To date,the virus has been reported to infect more than 11 cucurbit crops,in 16 countries and regions,causing severe yield losses.In autumn 2022,ToLCNDV was first isolated from cucurbit plants in Southeastern coastal areas of China.Phylogenetic analysis established that these isolates belong to the Asian ToLCNDV clade,and shared high nucleotide identity and closest genetic relationship with the DNA-A sequence from the Chinese tomato-infecting ToLCNDV isolate(Accession no.OP356207)and the tomato New Delhi ToLCNDV-Severe isolate(Accession no.HM159454).In this review,we summarize the occurrence and distribution,host range,detection and diagnosis,control strategies,and genetic resistance of ToLCNDV in the Cucurbitaceae.We then summarize pathways that could be undertaken to improve our understanding of this emerging disease,with the objective to develop ToLCNDV-resistant cucurbit cultivars. | Lingmin Cai Yuzhen Mei Ruyi Ye Yun Deng Xuejun Zhang Zhongyuan Hu Xueping Zhou Mingfang Zhang Jinghua Yang | 2023 | aBIOTECH2023，4，3： | 0 |
| 11 | Distribution Characteristics of Methylmercury in Eriocheir sinensis Produced in Zhejiang Province and the Risk of Eating It显示文摘Based the detection of content of methylmercury in the meat and butter of Eriocheir sinensis cultured in Zhejiang Province by liquid chromatography-atomic fluorescence spectroscopy,the distribution characteristics of methylmercury were analyzed,and the exposure risk of methylmercury to consumers was evaluated. The results showed that the content of methylmercury in the crab meat was higher than that of the crab butter. According to China's national standard and industry standard,the content of methylmercury in the crab meat was lower than the limit and accorded with relevant regulations. According to the reference intake of methylmercury recommended by the World Health Organization( WHO) and the United States Environmental Protection Agency( US EPA),the weekly intake of E. sinensis meat should not exceed 3.2 kg,and the daily intake should not exceed 200g. | Zhou Dongren Sun Boyi Hao Guijie Sheng Pengcheng Shi Qinxuan Xv Lei Luo Yizhi Wang Jun Ye Xueping | 2018 | Meteorological and Environmental Research2018，9，2： | 0 |
| 12 | Ultrasound microbubbles combined with liposome-mediated pNogo-R shRNA delivery into neural stem cells显示文摘In the present study, ultrasound-mediated microbubble destruction (UMMD) alone and combined with liposome technology was used as a novel nonviral technique to transfect a Nogo receptor (Nogo-R) shRNA plasmid (pNogo-R shRNA) into neural stem cells (NSCs). Using green fluorescent protein as a reporter gene, transfection efficiency of NSCs was significantly higher in the group transfected with UMMD combined with liposomes compared with that of the group transfected with UMMD or liposomes alone, and did not affect cell vitality. In addition, Nogo-R mRNA and protein expression was dramatically decreased in the UMMD combined with liposome-mediated group compared with that of other groups after 24 hours of transfection. The UMMD technique combined with liposomes is a noninvasive gene transfer method, which showed minimal effects on cell viability and effectively increased transfer of Nogo-R shRNA into NSCs. | Weixia Ye Xueping Huang Yangyang Sun Hao Liu Jin Jiang Youde Cao | 2012 | Neural Regeneration Research2012，7，1： | 0 |
| 13 | Plant virology in the 21st century in China:Recent advances and future directions显示文摘Plant viruses are a group of intracellular pathogens that persistently threaten global food security.Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years,including basic research and technologies for preventing and controlling plant viral diseases.Here,we review these milestones and advances,including the identification of new crop-infecting viruses,dissection of pathogenic mechanisms of multiple viruses,examination of multilayered interactions among viruses,their host plants,and virus-transmitting arthropod vectors,and in-depth interrogation of plantencoded resistance and susceptibility determinants.Notably,various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in plants.We also recommend future plant virology studies in China. | Jianguo Wu Yongliang Zhang Fangfang Li Xiaoming Zhang Jian Ye Taiyun Wei Zhenghe Li Xiaorong Tao Feng Cui Xianbing Wang Lili Zhang Fei Yan Shifang Li Yule Liu Dawei Li Xueping Zhou Yi Li | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
| 14 | Purification Effect of Trionyx sinensis Breeding Wastewater by a Synthetic Biological Pond显示文摘A synthetic biological pond composed of aquatic plants,a microorganism and aquatic animals was applied in the purification of the wastewater discharged from a Trionyx sinensis breeding greenhouse,and the removal effects of TN,NH_4^+-N,TP and COD in the wastewater were analyzed.The results show that after the breeding wastewater was purified by the synthetic biological pond for about five months,TN,NH_4^+-N,TP and COD concentration in the breeding wastewater were reduced by 78%,92%,81%and 60%respectively,while DO content in the breeding wastewater increased by about four times,and there was no obvious change in water pH;previous yellow or brown and turbid sewage became yellowish and slightly turbid,and it had no smell.It is obviously seen that the purification effect of T.sinensis breeding wastewater by the synthetic biological pond composed of aquatic plants,a microorganism and aquatic animals is obvious,and the synthetic biological pond has good promotion and application prospects. | Zhou Dongren Ye Xueping Liu Li Lin Feng Luo Yizhi Cao Zheng | 2015 | Meteorological and Environmental Research2015，6，8： | 0 |
| 15 | Isolation and Identification of the Pathogens of Bullfrog Streptoccus Disease显示文摘The tested strain from typical clinical cases,and the physio-chemical characteristics,16 S rRNA gene sequence and drug sensitivity challenge property were analyzed.This isolate was determined as Streptococcus agalactiae,16 S rRNA gene fragment of 1 280 bp in length.Blast analysis showed that this fragment shared a 100% homology with the conservative region of Streptococcus agalactiae isolate(AB023574),Streptococcus dysqalactiae isolate(HM146084.1,AB537922,EU075033),Streptococcus difficilis isolate(AB112407) accessed in Genbank.The isolate was sensitive to ciprofloxacin,neomycin,spectinomycin,furadantin and enoxacin.The results lay foundation for the effective control,rational medication and vaccine preparation for Streptococcus disease on bullfrogs. | Hang Xiaoying Zhou Dongren Luo Yizhi Shi Weida Ye Xueping | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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