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| 1 | Survival of transplanted neurotrophin-3 expressing human neural stem cells and motor function in a rat model of spinal cord injury显示文摘<正>BACKGROUND:Many methods have been attempted to repair nerves following spinal cord injury, including peripheral nerve transplantation,Schwann cell transplantation,olfactory ensheathing cell transplantation,and embryonic neural tissue transplantation.However,there is a need for improved outcomes. OBJECTIVE:To investigate the repair feasibility for rat spinal cord injury using human neural stem cells(hNSCs) genetically modified by lentivirus to express neurotrophin-3. DESIGN,TIME AND SETTING:In vitro cell biological experiment and in vivo randomized,controlled, genetic engineering experiment were performed at the Third Military Medical University of Chinese PLA and First People's Hospital of Yibin,China from March 2006 to December 2007. MATERIALS:A total of 64 adult,female,Wistar rats were used for the in vivo study.Of them,48 rats were used to establish models of spinal cord hemisection,and were subsequently equally and randomly assigned to model,genetically modified hNSC,and normal hNSC groups.The remaining 16 rats served as normal controls. METHODS:hNSCs were in vitro genetically modified by lentivirus to secrete both green fluorescence protein and neurotrophin-3.Neurotrophin-3 expression was measured by Western blot. Genetically modified hNSC or normal hNSC suspension(5×10~5) was injected into the rat spinal cord following T_(10) spinal cord hemisection.A total of 5μL Dulbecco's-modified Eagle's medium was infused into the rat spinal cord in the model group.Transgene expression and survival of transplanted hNSCs were determined by immunohistochemistry.Motor function was evaluated using the Basso,Beattie,and Bresnahan(BBB) scale. MAIN OUTCOME MEASURES:The following parameters were measured:expression of neurotrophin-3 produced by genetically modified hNSCs,transgene expression and survival of hNSCs in rats,motor function in rats. RESULTS:hNSCs were successfully genetically modified by lentivirus to stably express neurotrophin-3.The transplanted hNSCs primarily gathered at,or around,the injection site two weeks following transplantation,and gradually migrated towards the surrounding tissue. Transplanted hNSCs were observed 7.0-8.0 mm away from the injection site.In addition,hNSCs were observed 10 weeks after transplantation.At week 4,BBB locomotor scores were significantly greater in the genetically modified hNSC and normal hNSC groups,compared with the model group (P<0.05),and scores were significantly greater in the genetically modified hNSC group compared with the normal hNSC group(P<0.05). CONCLUSION:hNSCs were genetically modified with lentivirus to stably secrete neurotrophin-3. hNSCs improved motor function recovery in rats following spinal cord injury. | Peiqiang Cai Guangyun Sun Peishu Cai Martin Oudega Rui Xiao Xuewen Wang Wei Li Yunbing Shu Cheng Cai Haihao Yang Xuebing Shan Wuhua Luo | 2009 | Neural Regeneration Research2009，4，7： | 18 |
| 2 | Silicon-on-insulator-based microwave photonic filter with widely adjustable bandwidth显示文摘We demonstrate a silicon-based microwave photonic filter(MPF) with flattop passband and adjustable bandwidth. The proposed MPF is realized by using a 10 th-order microring resonator(MRR) and a photodetector,both of which are integrated on a photonic chip. The full width at half-maximum(FWHM) bandwidth of the optical filter achieved at the drop port of the 10 th-order MRR is 21.6 GHz. The ripple of the passband is less than 0.3 dB, while the rejection ratio is 32 dB. By adjusting the deviation of the optical carrier wavelength from the center wavelength of the optical bandpass filter, the bandwidth of the MPF can be greatly changed. In the experiment, the FWHM bandwidth of the proposed MPF is tuned from 5.3 to 19.5 GHz, and the rejection ratio is higher than 30 dB. | LU XU JIE HOU HAITAO TANG YUAN YU YU YU XUEWEN SHU XINLIANG ZHANG | 2019 | Photonics Research2019，7，2： | 5 |
| 3 | The static contact statuses between granular materials and flat- bottomed steel silos 显示文摘 | WAN Xuewen YANG Zhaojian SHU Xuefeng | 2013 | Powder Technology2013，235，： | 1 |
| 4 | Sensitivity characteristics of long - period fiber gratings 显示文摘 | Xuewen Shu Lin Zhang Bennion I | 2002 | Journal of Lightwave Technology2002，20，： | 1 |
| 5 | Sensitivity characteristics of long-period fiber gratings显示文摘 | Shu Xuewen Zhang Lin Ina Bennion | 2002 | Journal of Lightwave Technology2002，20，2： | 1 |
| 6 | Sensitivity characteristics of long-period fiber gratings显示文摘 | Zhang Lin Bennion I | 2002 | J Lightwave Technology2002，20，2： | 1 |
| 7 | Narrow-band generation in random distributed feedback fi- ber laser 显示文摘 | Srikanth Sugavanam Nikita Tarasov Xuewen Shu | 2013 | Optics Express2013，21，16： | 1 |
| 8 | Sensitivity characteristics of long- period fiber gratings显示文摘 | Xuewen Shu Lin Zhang Ian Bennion | 2002 | Journal of lightwave technology2002，20，25： | 1 |
| 9 | High-tem- perature sensitivity of long-period gratings in B-Ge co- doped fiber显示文摘 | Shu Xuewen Allsop T Gwandu B | 2001 | IEEE Photonics Technology Letters2001，13，8： | 1 |
| 10 | Fiber grating Sagnac loop and its multiwavelength-laser application显示文摘 | Xuewen Shu Shan Jiang Dexiu Huang | 2000 | IEEE photonics technology letters2000，8，12： | 1 |
| 11 | Fiber Grating Sagnac Loop and Its Multiwavelength-Laser Application显示文摘 | Xuewen Shu Shan Jiang Dexiu Huang | 2000 | IEEE2000，9，49： | 1 |
| 12 | Sampled fiber Bragg grating for simultaneous refractive-index and temperature measure- ment 显示文摘 | Shu Xuewen Gwandu B A L Liu Yu | 2001 | Optics Letters2001，11，： | 1 |
| 13 | Sensitivity char- act eristics of long period fiber gratings显示文摘 | Shu Xuewen Zhang Lin Bennion I | 2002 | Journal of Lightwave Technology2002，20，2： | 1 |
| 14 | Tailored Gires-Tournois etalons as tunable dispersion slope compensators 显示文摘 | Xuewen Shu Ian Bennion | 2004 | 29(9) :1013 - 10152004，29，9： | 1 |
| 15 | Sensitivity characteristics of long-period fiber gratings显示文摘 | Xuewen Shu Lin Zhang | 2002 | J Lightwave Technol2002，20，2： | 1 |
| 16 | Bragg-grating-based all-fiber distributed Gires-Toumois etalons显示文摘 | Xuewen Shu Kate Sugden Kevin Byron | 2003 | Opt Lett2003，28，11： | 1 |
| 17 | Sensitivity characteristics near the dispersion turning points of long-period fiber gratings in B/Ge eodoped fiber显示文摘 | Shu Xuewen Zhang Lin Bennion I | | Optics Letters0，26，22： | 1 |
| 18 | Mining rare and ubiquitous toxin genes from a large collection of Bacillus thuringien- sis strains 显示文摘 | Li Ying Shu Changlong Zhang Xuewen | 2014 | Journal of Invertebrate Pathology2014，122，： | 1 |
| 19 | Sensitivity characteristics of long-period tiber gratings显示文摘 | Xuewen Shu Lin Zhang I Bennion | 2002 | J Lgntwave ecnno2002，20，2： | 1 |
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