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| 1 | Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘 | Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou | 2014 | Cell Research2014，24，6： | 81 |
| 2 | Characterization of Panax ginseng UDP- Glycosyltransferases Catalyzing Protopanaxatriol and Biosyntheses of Bioactive Ginsenosides F1 and Rhl in Metabolically Engineered Yeasts显示文摘人参皂甙,在人参(人参属人参) 的主要 pharmacologically 活跃的自然混合物,主要是 protopanaxadiol (PPD ) 和 protopanaxatriol (PPT ) 的 glycosylated 产品。uridine diphosphate glycosyltransferase (UGT ) 催化 PPT 生产 PPT 类型人参皂甙,还都没被报导。这里,我们显示出那 UGTPg1,它 regio 明确地被表明了到 glycosylate C20 -- PPD 的 OH,另外明确地 glycosylates C20 -- PPT 到的 OH 生产 bioactive 人参皂甙 F1。我们报导四新奇 UGT 基因从 P.ginseng 孤立的 characterizationof,分享高推出的氨基酸身份(>84%) 与 UGTPg1。我们明确地表明那 UGTPg100 glycosylates C6 -- PPT 的 OH 催化 PPT 生产 bioactive 人参皂甙 Rh1,和 UGTPg101 生产 F1,从 F1 由人参皂甙 Rg1 的产生列在后面。然而, UGTPg102 和 UGTPg103 被发现不在 PPT 上举办可检测的活动。通过结构的建模和指导地点的 mutagenesis,我们识别了可以在决定他们的活动和底层 regio 特性起重要作用的这些 UGT 的几关键氨基酸。而且,我们由介绍遗传上设计的生产 PPT 小径和 UGTPg1 或 UGTPg100 构造了酵母 recombinants 到 biosynthesize F1 和 Rh1。我们的学习经由合成生物学策略在酵母为 bioactive PPT 类型人参皂甙在人参属工厂,和 providesa 声音生产途径揭示 PPT 类型人参皂甙的可能的 biosynthetic 小径。 | Wei Wei Pingping Wang Yongjun Wei Qunfang Liu Chengshuai Yang Guoping Zhao Jianmin Yue Xing Yan Zhihua Zhou | 2015 | Molecular Plant2015，8，9： | 37 |
| 3 | Concurrent intracranial and extracranial artery stenosis and the prognosis of transient ischaemic symptoms or imaging-negative ischaemic stroke显示文摘Background and purpose Transient ischaemic attack(TIA),transient symptoms with infarction(TSI)and diffusion-weighted imaging(DWI)-negative acute ischaemic stroke(AIS)share similar aetiologies but are considered to have a rather benign prognosis.We intended to investigate the association between intracranial atherosclerotic stenosis(ICAS),extracranial atherosclerotic stenosis(ECAS)and the prognosis of patients with TIA,TSI and DWI-negative AIS.Methods Clinical and imaging data of eligible participants were derived from the Chinese Intracranial Atherosclerosis study,according to symptom duration,acute infarction on DWI and discharge diagnosis.Based on the severity and location of arterial atherosclerosis,we categorised the study population into four groups:no or<50% ICAS and no ECAS;≥50% ICAS but no ECAS;no or<50%ICAS with ECAS;and concurrent≥50% ICAS and ECAS.Using multivariable Cox regression models,we analysed the relationship between the severity and distribution of large artery atherosclerosis and the prognosis of TIA,TSI and DWI-negative AIS.Results A total of 806 patients were included,67.3% of whom were male.The median age of the study participants was 63 years.Patients in the concurrent≥50% ICAS and ECAS subgroup had both a significantly higher 1-year recurrence rate(adjusted HR 3.4(95%CI 1.15 to 10.04),p=0.027)and a higher risk of composite vascular events(adjusted HR 3.82(95%CI 1.50 to 9.72),p=0.005).Conclusions Concurrent ICAS and ECAS is associated with a higher possibility of 1-year recurrent stroke or composite vascular events.Large artery evaluation is necessary to assess patients with transient ischaemic symptoms or DWI-negative AIS.Progress in shortening the time interval between symptom onset and large vessel evaluation is needed. | Yue Suo Jing Jing Yuesong Pan Weiqi Chen Hongyu Zhou Hao Li Yuehua Pu Liping Liu Xingquan Zhao Yilong Wang Xia Meng Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，1： | 10 |
| 4 | Redox-sensitive prodrug nanoassemblies based on linoleic acid-modified docetaxel to resist breast cancers显示文摘Prodrug nanoassemblies, which can refrain from large excipients, achieve higher drug loading and control drug release, have been placed as the priority in drug delivery system. Reasoning that glutathione(GSH) and reactive oxygen species(ROS) are highly upgraded in tumor tissues which makes them attractive targets for drug delivery system, we designed and synthetized a novel prodrug which utilized mono thioether bond as a linker to bridge linoleic acid(LA) and docetaxel(DTX). This mono thioether-linked conjugates(DTX-S-LA) could self-assemble into nanoparticles without the aid of much excipients. The mono thioether endowed the nanoparticles redox sensitivity resulting in specific release at the tumor tissue. Our studies demonstrated that the nanoassemblies had uniform particle size, high stability and fast release behavior. DTX-S-LA nanoassemblies outperformed DTX solution in pharmacokinetic profiles for it had longer circulation time and higher area under curve(AUC). Compared with DTX solution, the redox dual-responsive nanoassemblies had comparable cytotoxic activity. Besides, the antitumor efficacy was evaluated in mice bearing 4 T1 xenograft. It turned out this nanoassemblies couldenhance anticancer efficacy by increasing the dose because of higher tolerance. Overall, these results indicated that the redox sensitivity nanoassemblies may have a great potential to cancer therapy. | Meng Li Liwen Zhao Tao Zhang Yue Shu Zhonggui He Yan Ma Dan Liu Yongjun Wang | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 10 |
| 5 | Ratiometric delivery of doxorubicin and berberine by liposome enables superior therapeutic index than Doxil?显示文摘Although the appearance of Doxil alleviated the cardiotoxicity of DOX, the progression-free survival of patients was not prolonged compared with traditional medication regimens, and side effects such as hand-foot syndrome has occurred. In order to solve this dilemma, we have designed a novel co-delivery strategy to construct a co-loaded liposome of berberine(BER) and doxorubicin(DOX), which was called Lipo Be Do. The optimal synergistic ratio of the two drugs was screened by cell cytotoxicity experiments in vitro, and the optimal attenuation ratio was further determined by in vivo cardiac H&E staining pathological sections. The optimal combination treatment caused a robust increase in apoptotic cells of 4T1, as compared to drug alone treatment. The prepared co-loaded liposome, Lipo Be Do, had high encapsulation efficiency and good stability. The nanoliposome carrier controlled the biological fate of the drugs and maintained a pre-defined optimal ratio in vivo. The Lipo Be Do significantly inhibited tumor growth in 4T1 murine mammary carcinoma model compared with Doxil(P < 0.05), and completely overcame the myocardial rupture toxicity caused by Doxil in mice. Our co-loaded liposome delivery platform technology provided a new direction for the clinical treatment of triple-negative breast cancer and the safe application of DOX. | Ruoshi Zhang Yingxi Zhang Yue Zhang Xin Wang Xuanming Gao Yuyan Liu Xuanbo Zhang Zhonggui He Dun Wang Yongjun Wang | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，3： | 4 |
| 6 | SOCS3 Attenuates GM-CSF/IFN-γ-Mediated Inflammation During Spontaneous Spinal Cord Regeneration显示文摘SOCS3,a feedback inhibitor of the JAK/STAT signal pathway,negatively regulates axonal regrowth and inflammation in the central nervous system(CNS).Here,we demonstrated a distinct role of SOCS3 in the injured spinal cord of the gecko following tail amputation.Severing the gecko spinal cord did not evoke an inflammatory cascade except for an injury-stimulated elevation of the granulocyte/macrophage colony-stimulating factor(GM-CSF) and interferon gamma(IFN-γ) cytokines.Simultaneously,the expression of SOCS3 was upregulated in microglia,and unexpectedly not in neurons.Enforced expression of SOCS3 was sufficient to suppress the GMCSF/IFN-γ-driven inflammatory responses through its KIR domain by attenuating the activities of JAK1 and JAK2.SOCS3 was also linked to GM-CSF/IFN-y-induced crosstolerance.Transfection of adenovirus overexpressing SOCS3 in the injured cord resulted in a significant decrease of inflammatory cytokines.These results reveal a distinct role of SOCS3 in the regenerating spinal cord,and provide new hints for CNS repair in mammals. | Xuejie Zhang Bingqiang He Hui Li Yingjie Wang Yue Zhou Wenjuan Wang Tiancheng Song Nan Du Xingxing Gu Yi Luo Yongjun Wang | 2020 | Neuroscience Bulletin2020，36，7： | 3 |
| 7 | Vascular dementia has the highest hospitalisation rate in China:a nationwide hospital information system study显示文摘Background Although vascular dementia(VaD)is not uncommon in the hospital,most studies on VaD are community based.This study on VaD is the first to use a national hospital information system(HIS)in China.Methods This study was a hospital population-based cohort study,and data were acquired from the Hospital Quality Monitoring System,a Chinese national database that covers 1531 tertiary hospitals in China.The medical records in the HIS included demographic information,diagnoses,procedures,expenses,etc.VaD was diagnosed by hospital attending physicians and identified by the International Classification of Diseases(ICD)10 code(F01.0-F01.9).Results 1259(82.23%)tertiary hospitals were included in this study,and 274230 hospitalisation records of 123700 VaD patients from 2016 to 2018 were identified for analysis.VaD(51.27%)was the most common type of dementia in hospitalised patients.The age-adjusted and sex-adjusted hospital incidence of VaD was estimated to be 2.97 per 100000 person-years(95%CI 2.92 to 3.02).The mean age of VaD patients admitted to hospitals was 74.13±10.88,with more male(61.0%)patients.The main comorbidities were hypertension(67.2%),heart disease(63.6%)and cerebral infarction(55.5%).The mortality rate of VaD in hospital was 28.91‰(95%CI 28.0 to 29.9)between 2016 and 2018.The top 1 cause of death due to VaD was pneumonia and other respiratory disorders.Conclusions VaD is the most common form of dementia in hospitalised patients.It casts huge burdens on affected patients and their caregivers.Vascular risk factors are prevalent in VaD patients.Reducing these factors is essential to improve patient care.The leading causes of death by VaD are pneumonia and other respiratory disorders.Hospital care and the management of respiratory illness are critical for VaD care in the hospital. | Yue Wang Shiping Li Qi Zhou Yongjun Wang Jiong Shi | 2023 | Stroke & Vascular Neurology2023，8，1： | 2 |
| 8 | Initiation and longterm slip history of the Altyn Tagh fault显示文摘 | Yue Yongjun Bradley D Ritts Stephan A Graham | 2001 | International Geology Review2001，43，： | 1 |
| 9 | Facile Synthesis of Fe3Pt-Ag Nanocomposites for Catalytic Reduction of Methyl Orange显示文摘 | HAN Donglai LI Boxun XING Guoliang ZHANG Yuanyuan CHEN Yue SUN Yantao ZHANG Yongjun LIU Yang YANG Jinghai | 2018 | Chemical Research in Chinese Universities2018，34，6： | 1 |
| 10 | Association between plasma homocysteine levels and obstructive sleep apnoea in patients with ischaemic stroke显示文摘 | Maogang Chen Bona Wu Xinchun Ye Zhiming Zhou Xuanye Yue Qizhang Wang Wanxiang Wang Yongjun Jiang Xuegan Lian Zhaoyao Chen Gelin Xu Xinfeng Liu | 2011 | Journal of Clinical Neuroscience2011，，11： | 1 |
| 11 | Effect of sex differences on prognosis of intravenous thrombolysis: data from the Thrombolysis Implementation and Monitor of Acute Ischemic Stroke in China (TIMS- China)显示文摘Background and purpose Previous studies have reported conflicting results as to whether women have poorer functional outcome than men after thrombolytic therapy.This study aims to investigate the relationship between sex differences and the prognosis of intravenous thrombolysis in Chinese patients with acute ischaemic stroke.Methods The patients enrolled in this study were from the Chinese Acute Ischemic Stroke Thrombolysis Monitoring and Registration study.The primary outcome was poor functional outcome,defined as a 3-month modified Rankin score of 3-6.The safe outcome was symptomatic intracranial haemorrhage(SICH)and mortality within 7 days and 90 days.Multiple Cox regression model was used to correct the potential covariates to evaluate the association between sex disparities and prognosis.Furthermore,the interaction of preonset Rankin scores,baseline National Institute of Health Stroke Scale(NIHSS)scores and Trial of Org 10172 in Acute Stroke Treatment(TOAST)types was statistically analysed.results A total of 1440 patients were recruited,including 541 women and 899 men.The baseline information indicated that women were older at the time of onset(66.2±11.2 years vs 61.0±11.3 years,p<0.001),and more likely to have a history of atrial fibrillation(25.3%vs 11.2%,p<0.001),and had a higher NIHSS score on admission(12.3±6.8 vs 11.6±6.7,p=0.04).According to the prognosis analysis of unsatisfactory functional recovery,there was no significant difference between women and men(45.9%vs 37.1%;adjusted OR 1.01,95%CI 0.75 to 1.37).As for the safe outcome,the proportion of SICH and mortality in women is relatively high but did not reach statistical significance.There was no significant interaction with sex,age,preonset Rankin score,NIHSS score,TOAST classification and the prognosis of intravenous thrombolysis.Conclusions For Chinese patients with ischaemic stroke,although women are older and more severe at the time of onset,the prognosis after intravenous thrombolysis is not significantly different from men. | Hongyu Zhou Weiqi Chen Yuesong Pan Yue Suo Xia Meng Hao Li Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，1： | 1 |
| 12 | Two-stage evolution model for the Altyn Tagh Fault, China显示文摘 | YUE Yongjun JUHN G L | 1999 | Geology1999，27，3： | 1 |
| 13 | Two-stage evolution model for the Altyn Tagh fault, China显示文摘 | Yue Yongjun Liou J | 1999 | Geology1999，27，： | 1 |
| 14 | Initiation and long-term slip history of the Altyn Tagh fault显示文摘 | Yue Yongjun Ritts B D Graham S A | 2001 | International Geology Review2001，43，12： | 1 |
| 15 | Two-stage evolution model for the Altyn Tagh fault, China显示文摘 | Yue Yongjun Liou J G | 1999 | Geology1999，27，3： | 1 |
| 16 | Two stage evolution model for the Altyn Tagh fault, China显示文摘 | John G Liou | 1999 | Geology1999，27，3： | 1 |
| 17 | Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury显示文摘Astrocytes are important cellular centers of cholesterol synthesis and metabolism that help maintain normal physiological function at the organism level.Spinal cord injury results in aberrant cholesterol metabolism by astrocytes and excessive production of oxysterols,which have profound effects on neuropathology.25-Hydroxycholesterol(25-HC),the main product of the membrane-associated enzyme cholesterol-25-hydroxylase(CH25H),plays important roles in mediating neuroinflammation.However,whether the abnormal astrocyte cholesterol metabolism induced by spinal cord injury contributes to the production of 25-HC,as well as the resulting pathological effects,remain unclear.In the present study,spinal cord injury-induced activation of thrombin was found to increase astrocyte CH25H expression.A protease-activated receptor 1 inhibitor was able to attenuate this effect in vitro and in vivo.In cultured primary astrocytes,thrombin interacted with protease-activated receptor 1,mainly through activation of the mitogen-activated protein kinase/nuclear factor-kappa B signaling pathway.Conditioned culture medium from astrocytes in which ch25h expression had been knocked down by siRNA reduced macrophage migration.Finally,injection of the protease activated receptor 1 inhibitor SCH79797 into rat neural sheaths following spinal cord injury reduced migration of microglia/macrophages to the injured site and largely restored motor function.Our results demonstrate a novel regulatory mechanism for thrombin-regulated cholesterol metabolism in astrocytes that could be used to develop anti-inflammatory drugs to treat patients with spinal cord injury. | Chen Chen Huiyuan Ji Nan Jiang Yingjie Wang Yue Zhou Zhenjie Zhu Yuming Hu Yongjun Wang Aihong Li Aisong Guo | 2023 | Neural Regeneration Research2023，18，6： | 1 |
| 18 | Initiation and long-term slip history of the Altyn Tagh fault显示文摘 | Bradley D Ritts Stephan A Graham | 2001 | International Geology Review2001，43，： | 1 |
| 19 | Oligocene-Miocene tectonics and sedimentation along the Altyn Tagh fault,northern Tibetan plateau: analysis of the Xorkol, Subei, and Aksay basins显示文摘 | RITTS B D YUE Yongjun GRAHAM S A | 2004 | Journal of Geology2004，112，： | 1 |
| 20 | Gene panel for Mendelian strokes显示文摘background Mendelian stroke causes nearly 7% of ischaemic strokes and is also an important aetiology of cryptogenic stroke.Identifying the genetic abnormalities in Mendelian strokes is important as it would facilitate therapeutic management and genetic counselling.Next-generation sequencing makes large-scale sequencing and genetic testing possible.Methods A systematic literature search was conducted to identify causal genes of Mendelian strokes,which were used to construct a hybridization-based gene capture panel.Genetic variants for target genes were detected using Illumina HiSeq X10 and the Novaseq platform.The sensitivity and specificity were evaluated by comparing the results with Sanger sequencing.results 53 suspected patients of Mendelian strokes were analysed using the panel of 181 causal genes.According to the American College of Medical Genetics and Genomics standard,16 likely pathogenic/variants of uncertain significance genetic variants were identified.Diagnostic testing was conducted by comparing the consistency between the results of panel and Sanger sequencing.Both the sensitivity and specificity were 100%for the panel.Conclusion This panel provides an economical,time-saving and labour-saving method to detect causal mutations of Mendelian strokes. | Fang Fang Zhe Xu Yue Suo Hui Wang Si Cheng Hao Li Wei Li Yongjun Wang | 2020 | Stroke & Vascular Neurology2020，5，4： | 1 |
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