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| 1 | Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘 | Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou | 2014 | Cell Research2014，24，6： | 81 |
| 2 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 3 | Geodynamic significance of the A-type granites in the Sawuer region in west Junggar, Xinjiang: Rock geochemistry and SHRIMP zircon age evidence显示文摘Sawuer region is located in west Junggar, Jimunai County of Altay district and Hefeng County of Tacheng district, Xinjiang. The region is along the north margin of Kazakstan-Junggar plate. The intrusions (mainly acid) are widespread. Qiaqihai and Kuoyitasi intrusions are important in the region, with the characteristics of A-type granite. Further investigations indicate that they belong to A2-type granite that formed in extension tectonic setting of post collision. The REE chon-drite-normalized patterns of the intrusions show LREE enrichment and the δ Eu values are lower. The Nd, Sr, Pb isotope compositions of the intrusions indicate a mantle source, while the low δ 18O values resulted from the isotope exchange between intrusion and meteoric water. According to SHRIMP U-Pb age analysis results, the crystallization age of Qiaqihai intrusion is 290.7 ± 9.3 Ma (1σ ), and that of Kuoyitasi intrusion is 297.9 ± 4.6 Ma (1σ ), corresponding to the beginning of early Permian. The A2-type granites indicate that the region was in the extension period of the post-collisional stage at the beginning of early Permian in the Sawuer region. The A-type granites in the Sawuer region in west Junggar discovered from this work is analogous to the A-type granites found in east Junggar of the Ulungur alkali granites belt. The confirmation of post-collisional A-type granites of early Permian in the Sawuer region provides new evidence for the regional vertical continental crust growth in early Per-mian. The former proposed Ulungur alkali granites belt can extend from east Ulungur through west Ulungur and to Zhaisang in Kazakstan westwards. | ZHOU Taofa1, YUAN Feng1, TAN Lugui1,2, FAN Yu1 & YUE Shucang1 1. School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China 2. Department of Geography and Environment Science, Wanxi College, Liuan 237012, China | 2006 | Science China Earth Sciences2006，49，2： | 42 |
| 4 | Mortality and Morbidity of Extremely Low Birth Weight Infants in the Mainland of China: A Multi-center Study显示文摘 | Hui-Jia Lin Li-Zhong Du Xiao-Lu Ma Li-Ping Shi Jia-Hua Pan Xiao-Mei Tong Qiu-Ping Li Jian-Guo Zhou Bing Yi Ling Liu Yun-Bing Chen Qiu-Fen Wei Hui-Qing Wu Mei Li Cui-Qing Li Xi-Rong Gao Shi-Wen Xia Wen-Bin Li Chao-Ying Ya Ling He Kun Liang Xiao-Yu Zhou Shu-Ping Han Qin Lyu Yin-Ping Qiu Wen Li Dong-Mei Chen Hong-Ru Lu Xiao-Hong Liu Hong Liu Zhen-Lang Lin Li Liu Jia-Jun Zhu Hong Xiong Shao-Jie Yue Si-Qi Zhuang | 2015 | Chinese Medical Journal2015，，20： | 49 |
| 5 | Clinical efficacy of three different minimally invasive procedures for far lateral lumbar disc herniation显示文摘 | LIU Tao ZHOU Yue WANG Jian CHU Tong-wei LI Chang-qing ZHANG Zheng-feng ZHANG Chao ZHENG Wen-jie | 2012 | Chinese Medical Journal2012，，6： | 35 |
| 6 | Colorectal cancer lymph node staining by activated carbon nanoparticles suspension in vivo or methylene blue in vitro显示文摘AIM:To investigate whether activated carbon nanoparticles suspension(ACNS) or methylene blue(MB) can increase the detected number of lymph nodes in colorectal cancer.METHODS:Sixty-seven of 72 colorectal cancer patients treated at our hospital fulfilled the inclusion criteria of the study which was conducted from December 2010 to February 2012.Seven patients refused to participate.Eventually,60 patients were included,and randomly assigned to three groups(20 in each group):ACNS group(group A),MB group(group B) and non-stained conventional surgical group(group C).In group A,patients received subserosal injection of 1 mL ACNS in a 4-quadrant region around the mass.In group B,the main artery of specimen was identified and isolated after the specimen was removed,and 2 mL MB was slowly injected into the isolated,stretched and fixed vessel.In group C,no ACNS and MB were injected.All the mesentery lymph nodes were isolated and removed systematically by visually inspecting and palpating the adipose tissue.RESULTS:No difference was observed among the three groups in age,gender,tumor location,tumor diameter,T-stage,degree of differentiation,postoperative complications and peritoneal drainage retention time.The total number of detected lymph nodes was 535,476 and 223 in the three groups,respectively.The mean number of detected lymph nodes per patient was significantly higher in group A than in group C(26.8 ± 8.4 vs 12.2 ± 3.2,P < 0.001).Similarly,there were significantly more lymph nodes detected in group B than in group C(23.8 ± 6.9 vs 12.2 ± 3.2,P < 0.001).However,there was no significant difference between group A and group B.There were 50,46 and 32 metastatic lymph nodes dissected in 13 patients of group A,10 patients of group B and 11 patients of group C,without significant differences among the three groups.Eleven of the 60 patients had insufficient number of detected lymph nodes(< 12).Only one patient with T 4a rectal cancer had 10 lymph nodes detected in group B,the other 10 patients were all from group C.Based on the different diameter categories,the number of detected lymph nodes in groups A and B was significantly higher than in group C.However,there was no statistically significant difference between group A and group B.The metastatic lymph nodes were not significant different among the three groups.Similarly,tumor location,T stage and tumor differentiation did not affect the staining results.Body mass index was a minor influencing factor in the two different staining methods.The stained lymph nodes can easily be identified from the mesenteric adipose tissues,and the staining time for lymph nodes was not significantly different compared with unstained group.None of the patients in groups A and B had drug-related complications.CONCLUSION:Both activated carbon nanoparticles suspension in vivo and methylene blue in vitro can be used as tracers to increase the detected number of lymph nodes in colorectal cancer. | Hong-Ke Cai Hai-Fei He Wei Tian Mei-Qi Zhou Yue Hu Yong-Chuan Deng | 2012 | World Journal of Gastroenterology2012，18，42： | 39 |
| 7 | A High-Density SNP Genotyping Array for Rice Biology and Molecular Breeding显示文摘一个高密度的单个核苷酸多型性(SNP ) 数组为遗传学者和分子的 breeders.With 是极其重要的 genomic 的巨大的数量的累积重新定序为精确 SNP 察觉的数据和可得到的技术,设计高密度、高质量的米饭 SNP 数组是可能的。这里,我们报导一个高密度的 riceSNP 数组和它的实用程序的开发。SNP 探针被屏蔽超过 10 设计从变化和一个数组说出 RiceSNP50 的 801 米饭的 re-sequencingdata 提取的 000 000 SNP loci 在 Illumina Infinium 站台上被生产。数组 contained51 478 个均匀地分布式的标记,其 68% 个在遗传因子的区域以内。有 parent/F1 relationshipswere 的几百米饭植物过去常为精确 SNP 打电话产生一个高质量的簇文件。应用程序测试证明这穿有的 highgenotyping 精确性,并且能被用于不同目的。例如,有好分辨率的精英米饭变化 wasclustered 的一个核心集合。染色体宽的协会研究(GWAS ) 分析正确地识别了描绘的 QTL.Further,这个数组成功地为变化确认和特点基因渗入被使用。作为一个精确 high-throughputgenotyping 工具, RiceSNP50 将在两功能的 genomics 学习和分子的 breeding.Key 词起一个重要作用: | Haodong Chen Weibo Xie Hang He Huihui Yu Wei Chen Jing Li Renbo Yu Yue Yao Wenhui Zhang Yuqing He Xiaoyan Tang Fasong Zhou Xing Wang Deng Qifa Zhang | 2014 | Molecular Plant2014，7，3： | 38 |
| 8 | Characterization of Panax ginseng UDP- Glycosyltransferases Catalyzing Protopanaxatriol and Biosyntheses of Bioactive Ginsenosides F1 and Rhl in Metabolically Engineered Yeasts显示文摘人参皂甙,在人参(人参属人参) 的主要 pharmacologically 活跃的自然混合物,主要是 protopanaxadiol (PPD ) 和 protopanaxatriol (PPT ) 的 glycosylated 产品。uridine diphosphate glycosyltransferase (UGT ) 催化 PPT 生产 PPT 类型人参皂甙,还都没被报导。这里,我们显示出那 UGTPg1,它 regio 明确地被表明了到 glycosylate C20 -- PPD 的 OH,另外明确地 glycosylates C20 -- PPT 到的 OH 生产 bioactive 人参皂甙 F1。我们报导四新奇 UGT 基因从 P.ginseng 孤立的 characterizationof,分享高推出的氨基酸身份(>84%) 与 UGTPg1。我们明确地表明那 UGTPg100 glycosylates C6 -- PPT 的 OH 催化 PPT 生产 bioactive 人参皂甙 Rh1,和 UGTPg101 生产 F1,从 F1 由人参皂甙 Rg1 的产生列在后面。然而, UGTPg102 和 UGTPg103 被发现不在 PPT 上举办可检测的活动。通过结构的建模和指导地点的 mutagenesis,我们识别了可以在决定他们的活动和底层 regio 特性起重要作用的这些 UGT 的几关键氨基酸。而且,我们由介绍遗传上设计的生产 PPT 小径和 UGTPg1 或 UGTPg100 构造了酵母 recombinants 到 biosynthesize F1 和 Rh1。我们的学习经由合成生物学策略在酵母为 bioactive PPT 类型人参皂甙在人参属工厂,和 providesa 声音生产途径揭示 PPT 类型人参皂甙的可能的 biosynthetic 小径。 | Wei Wei Pingping Wang Yongjun Wei Qunfang Liu Chengshuai Yang Guoping Zhao Jianmin Yue Xing Yan Zhihua Zhou | 2015 | Molecular Plant2015，8，9： | 37 |
| 9 | Autotrophic denitrification for nitrate and nitrite removal using sulfur-limestone显示文摘Sulfur-limestone was used in the autotrophic denitrification process to remove the nitrate and nitrite in a lab scale upflow biofilter.Synthetic water with four levels of nitrate and nitrite concentrations of 10,40,70 and 100 mg N/L was tested.When treating the low concentration of nitrate-or nitrite-contaminated water(10,40 mg N/L),a high removal rate of about 90% was achieved at the hydraulic retention time(HRT) of 3 hr and temperature of 20-25°C.At the same HRT,50% of the nitrate or nitrite could be removed even at the low temperature of 5-10°C.For the higher concentration nitrate and nitrite(70,100 mg N/L),longer HRT was required.The batch test indicated that influent concentration,HRT and temperature are important factors afiecting the denitrification eficiency.Molecular analysis implied that nitrate and nitrite were denitrified into nitrogen by the same microorganisms.The sequential two-step-reactions from nitrate to nitrite and from nitrite to the next-step product might have taken place in the same cell during the autotrophic denitrification process. | Weili Zhou Yejue Sun Bingtao Wu Yue Zhang Min Huang Toshiaki Miyanaga Zhenjia Zhang | 2011 | Journal of Environmental Sciences2011，23，11： | 34 |
| 10 | Effect of hepatocyte apoptosis induced by TNF-α on acute severe hepatitis in mouse models显示文摘AIM To study the effect of hepatocyteapoptosis and necrosis induced by TNF-α on thepathogenesis of acute severe hepatitis(ASH).METHODS The model of ASH was prepared inD-galactosamine(GAIN)sensitized BALB/c miceby injection of either endotoxin(ET)or tumornecrosis factor-α(TNF-α).Morphologicalchanges of apoptotic hepatocytes were studiedby both light and electron microscope and in siteend labeling method(ISEL).Molecular biologicalchanges of DNA ladder were observed byelectrophoresis of extract from liver tissues.Biochemical changes were measured by alanineaminotransferase(ALT),asparticaminotransferase(AST)and TNF-α.The relationbetween apoptosis and necrosis was evaluatedsimultaneously.RESULTS The sequence of hepatocyteapoptosis,necrosis,and final death from ASHwas observed both in GAIN/ET and GAIN/TNF-agroup.Apoptosis was prominent at 3.5 h and 5 hafter injection of inducer,while necrosis becamedominant at 9 h after challenge.The appearanceof apoptosis was earlier in GAIN/TNF-α groupthan that in GAIN/ ET group.Pretreatment ofmice with antiTNF IgG1 may completely preventthe liver injury induced by GalN/ET.CONCLUSION TNF-α can cause liver damageby inducing hepatic apoptosis and necrosis inmice with endotoxemia. | Guo Qing Zang Xia Qiu Zhou Hong Yu Qing Xie Guo Ming Zhao Bin Wang Qing Guo Yue Qin Xiang Dan Liao Department of Infectious Diseases,Rujin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2000 | World Journal of Gastroenterology2000，6，5： | 30 |
| 11 | MicroRNA-125b Induces Cancer Cell Apoptosis Through Suppression of Bcl-2 Expression显示文摘MicroRNAs(miRNAs) are small,noncoding RNAs which can often act as an oncogene or a tumor suppressor.Several miRNAs are associated with the development of hepatocellular carcinoma(HCC).We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein,Bcl-2.Bioinformatic analysis indicated that the 3'UTR of Bcl-2 has binding sites for miR-125b.Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription,suggesting that Bcl-2 is a target gene for miR-125b.We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer cell apoptosis. | Aihua Zhao Quan Zeng Xiaoyan Xie Junnian Zhou Wen Yue Yali Li Xuetao Pei | 2012 | Journal of Genetics and Genomics2012，39，1： | 30 |
| 12 | Safflor yellow A protects neonatal rat cardiomyocytes against anoxia/reoxygenation injury in vitro显示文摘 | Jia-lin DUAN Jing-wen WANG Yue GUAN Ying YIN Guo WEI Jia CUI Dan ZHOU Yan-rong ZHU Wei QUAN Miao-miao XI Ai-dong WEN | 2013 | Acta Pharmacologica Sinica2013，34，4： | 27 |
| 13 | Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer's patches显示文摘AIM: To explore probable mechanism underlying the therapeutic effect of Astragalus polysaccharide(APS) against experimental colitis.METHODS: Thirty-two Sprague-Dawley rats were randomly divided into four groups. Colitis was induced with 2, 4, 6-trinitrobenzene sulfonic acid(TNBS). The rats with colitis were treated with 400 mg/kg of APS for 7 d. The therapeutic effect was evaluated by colonic weight, weight index of the colon, colonic length, and macroscopic and histological scores. The levels of regulatory T(Treg) cells in Peyer's patches were measured by flow cytometry, and cytokines in colonic tissue homogenates were analyzed using enzyme-linked immunosorbent assay. The expression of related orphan receptor-gt(ROR-gt), IL-23 and STAT-5a was measured by Western blot.RESULTS: After 7-d treatment with APS, the weight index of the colon, colonic weight, macroscopical and histological scores were decreased, while the colonic length was increased compared with the model group. The expression of interleukin(IL)-2, IL-6, IL-17, IL-23 and ROR-gt in the colonic tissues was down-regulated, but Treg cells in Peyer's patches, TGF-β and STAT5 a in the colonic tissues were up-regulated.CONCLUSION: APS effectively ameliorates TNBSinduced experimental colitis in rats, probably through restoring the number of Treg cells, and inhibiting IL-17 levels in Peyer's patches. | Hai-mei Zhao Yan Wang Xiao-Ying Huang min-fang Huang Rong Xu Hai-Yang Yue Bu-gao Zhou Hong-Yan Huang Qi-meng Sun Duan-Yong Liu | 2016 | World Journal of Gastroenterology2016，22，11： | 28 |
| 14 | Expression and function of classical protein kinase C isoenzymes in gastric cancer cell line and its drugresistant sublines显示文摘AIM: To investigate the expression and function of classicalprotein kinase C (PKC) isoenzymes in inducing MDRphenotype in gastric cancer cells.METHODS: Two cell lines were used in the study: gastriccancer cell SGC7901 and its drug-resistant cell SGC7901/VCRstepwise-selected by vincristine 0.3, 0. 7 and 1.0 mg@ L-1 ,respectively. The expression of classical PKC (cPKC)isoenzymes in SGC7901 cells and SGC7901/VCR cells weredetected using immunofluorescent cytochemistry, laserconfocal scanning microscope and Wsstern blot. The effectsof anti-PKC isoenzymes antibody of adriamycinaccumulation in SGC7901/VCR cells were determined usingflow cytometric analysis.RESULTS: (1) SGC7901 cells exhibited positive staining ofPKC-α. SGC7901/VCR cells exhibited stronger staining ofPKC-α than SGC7901 cells. The higher dosage vincristineselected, the much stronger staining of PKC-α was observedon SGC7901/VCR cells. (2) Both SGC7901 and SGC7901/VCRcells exhibited positive staining of PKC-βⅠ and PKC-βⅡ withno significant difference. ( 3 ) Compared with SGC7901,SGC7901/VCR cells had decreased adriamycin accumulationand retention. Accumulation of adriamycin in SGC7901 was5.21 + 2.56 mg@ L-1, in SGC7901/VCR 0.3 was 0.85 + 0.29 mg@L-1 , in SGC7901/VCR 0.7 was 0.81 + 0.32 og@ L-1 , and inSGC7901NCR 1.0 was 0.80 + 0.33 mg @ L-1; Retention ofadriamycin in SGC 7901 was 2.51 + 1.23 mg@L-1, in SGC7901/VCR 0.3 was 0.47 + 0.14 mg@ L-1 , in SGC7901/VCR 0.7 was 0.44 + 0.15 mg@ L-1, and in SGC 7901/VCR 1.0 was 0.41 + 0.1 1mg @ L-1 . (4) Fluorescence intensity presented adriamycinaccumulation in SGC7901/VCR cells was increased from 1.14+0.36 to 2.71 +0.94 when cells were co-incubated with anti-PKC-αbut not with anti-PKC-βⅠ, PKC-βⅡ and PKCγ antibodies.CONCLUSION: PKC-α, but not PKC-βⅠ, PKC-βⅡ or PKCγ,may play a role in multidrug resistance of gastric cancercells SGC7901/VCR. | Ying Han Zhe-Yi Han Xin-Min Zhou Ru Shi Yue Zheng Yong-Quan Shi Ji-Yan Miao Bo-Rong Pan Dai-Ming Fan | 2002 | World Journal of Gastroenterology2002，8，3： | 25 |
| 15 | Regulatory effect of a Chinese herbal medicine formula on nonalcoholic fatty liver disease显示文摘BACKGROUND Non-alcoholic fatty liver disease(NAFLD)has become a major cause of chronic liver disease.The Chinese herbal medicine(CHM)Dachaihu decoction(DCHD)has been proved to treat NAFLD with good efficacy in previous studies.Based on the TCM principle of formula formation,we divided DCHD into soothing liver part,invigorating spleen part,and dredging intestine part.Marshall officially proposed the concept of“intestinal-hepatic axis”,which systematically explains the interactions between the intestine and liver.We hypothesized that the effect of CHM on NAFLD is achieved by regulating the liver and intestine.Thus,we aimed to investigate the possible effect of a CHM formula on NAFLD in a rat model.AIM To investigate the effects of a CHM formula(a decoction of Chinese thorowax root,scutellaria root,and white peony root)on NAFLD and its regulatory effect on the“intestinal-liver”axis.METHODS Sixty rats were randomly divided into control,model,pioglitazone hydrochloride(PH),and CHM(a decoction of Chinese thorowax root,scutellaria root,and white peony root)groups.An NAFLD rat model was established using a high-fat highfructose diet for 16 wk.From the 13th week,rats were administered with PH or a decoction of Chinese thorowax,scutellaria,and white peony root(CHM group)for 4 wk.Rats in the control group and model group were administered with an equal volume of distilled water.At the end of the study,blood was collected via the abdominal aorta.Liver tissues were harvested and any morphological changes were observed by hematoxylin-eosin(HE)staining,Oil red O staining,and Masson staining.In addition,blood lipids,liver function markers,and triglyceride(TG)in liver tissues were analyzed.The levels of transforming growth factor-β1(TGF-β1),tumor necrosis factor-α(TNF-α),Toll-like receptor-4(TLR4),and nuclear factor-kappa B(NF-кB)in liver tissues and secreted immunoglobulin A(sIgA)in intestinal tissues were analyzed by ELISA,and protein and mRNA expression of occludin and zonula occludens-1(ZO-1)in the intestine were measured using Western blot and reverse transcriptionquantitative polymerase chain reaction,respectively.The endotoxin level in plasma was detected by endpoint chromogenic assay.RESULTS Compared to the normal control group,the liver coefficient,serum TG,total cholesterol(TC),low density lipoprotein(LDL),aspartate aminotransferase(AST),and alanine aminotransferase(ALT),blood glucose,plasma endotoxin,and the levels of TG,TNF-α,TGF-β,NF-kB,and TLR4 in liver tissues increased significantly in the model group,while serum high density lipoprotein(HDL),intestinal sIgA,and protein and mRNA expression of occludin and ZO-1 decreased significantly in the model group(P<0.01).PH and CHM attenuated the elevated liver coefficient,serum TG,TC,LDL,AST,and ALT,blood glucose,plasma endotoxin,and the levels of TG,TNF-α,TGF-β,NF-kB,and TLR4 in liver tissues and increased serum HDL levels compared to the model group(P<0.01).Intestinal sIgA and the protein and mRNA expression of intestinal occludin and ZO-1 were significantly increased in the PH group compared to the model and CHM groups(P<0.01).CONCLUSION The decoction of Chinese thorowax root,scutellaria root,and white peony root is beneficial in regulating lipid metabolism and liver function,which indicates that it has a good effect on the liver.To a certain extent,this CHM formula can affect both the liver and intestine,while its effect on the liver is superior to that on the intestine. | Jia-Min Yang Yan Sun Min Wang Xin-Lei Zhang Shu-Jing Zhang Yu-Shan Gao Lin Chen Meng-Yao Wu Lu Zhou Yu-Mei Zhou Yue Wang Feng-Jie Zheng Yu-Hang Li | 2019 | World Journal of Gastroenterology2019，25，34： | 24 |
| 16 | The FTO/miR-181b-3p/ARL5B signaling pathway regulates cell migration and invasion in breast cancer显示文摘Background:N6-methyladenosine(m6A)RNA modification has been demonstrated to be a significant regulatory process in the progression of various tumors,including breast cancer.Fat mass and obesity-associated(FTO)enzyme,initially known as the obesity-related protein,is the first identified m6A demethylase.However,the relationship between FTO and breast cancer remains controversial.In this study,we aimed to elucidate the role and clinical significance of FTO in breast cancer and to explore the underlying mechanism.Methods:We first investigated the expression of FTO in breast cancer cell lines and tissues by quantitative reverse transcription-PCR(qRT-PCR),Western blotting,and immunohistochemistry.Wound healing assay and Transwell assay were performed to determine the migration and invasion abilities of SKBR3 and MDAMB453 cells with either knockdown or overexpression of FTO.RNA sequencing(RNA-seq)was conducted to decipher the downstream targets of FTO.qRT-PCR,luciferase reporter assay,and Western blotting were employed to confirm the existence of the FTO/miR-181b-3p/ARL5B axis.The biological function of ADP ribosylation factor like GTPase 5B(ARL5B)in breast cancer cells was evaluated by wound healing assay and Transwell invasion assay.Results:High FTO expression was observed in human epidermal growth factor receptor 2(HER2)-positive breast cancer,predicting advanced progression(tumor size[P<0.001],nuclear grade[P=0.001],peritumoral lymphovascular invasion[P<0.001),lymph node metastasis[P=0.002],and TNM stage[P=0.001])and poor prognosis.Moreover,FTO promoted cell invasion and migration in vitro.Mechanistically,RNA-seq and further confirmation studies suggested that FTO up-regulated ARL5B by inhibiting miR-181b-3p.We further verified that ARL5B also displayed carcinogenic activity in breast cancer cells.Conclusion:Our work demonstrated the carcinogenic activity of FTO in promoting the invasion and migration of breast cancer cells via the FTO/miR-181b-3p/ARL5B signaling pathway. | Yuanyuan Xu Shuang Ye Nan Zhang Shuhui Zheng Huatao Liu Kewen Zhou Ling Wang Yue Cao Peng Sun Tinghuai Wang | 2020 | Cancer Communications2020，40，10： | 24 |
| 17 | Intracoronary nitroprusside in the prevention of the no-reflow phenomenon in acute myocardial infarction显示文摘背景没有回流现象在为尖锐心肌的梗塞(AMI ) 的经皮的冠的干预(一种总线标准) 期间是连续心肌的局部缺血,室的改变和心脏的机能障碍的一个预兆的因素,它仔细与更坏的预后被联系。这研究试图在有 AMI 的 92 个连续病人,在 12 小时发作以内经历了主要一种总线标准,随机被分到 2 个组的 AMI.Methods 在没有回流现象的预防评估 intracoronary nitroprusside:nitroprusside 的 intracoronary 管理(组 A, n=46 ) ,硝化甘油的 intracoronary 管理(组 B, n=46 ) 。angiographic 结果被观察。即时心肌的对比 echocardiography ( RT-MCE )包括对比 20 索引( CSI ),围运动 20 索引( WMSI ), transmural 对比缺点长度( CDL )和严肃的 WM 反常长度( WML )在 24 个小时和 1 星期 post-PCI.High 敏感被记录C反应的蛋白质( Hs-CRP )被有免疫力的率用悬液计测量悬液检验。N 终端 prohormone 大脑 natriu retic 肽(NT-proBNP ) 与连接酶的 immunosorbent 试金被测试。病人们被跟随在上面为六个月。主要不利心脏的事件(向) 在组 B 比那高是在组 A 的最后的 TIMI-3 流动的发生多是的 recorded.Results (P < 0.05 ) ,在组 A 的最后的改正的 TIMI 框架计数(cTFC ) 在组 B 比那显著地减少了(P < 0.01 ) 。在组 A 的 CSI, CDL/LV 长度, WMSI 和 WL/LV 长度在组 B 是比那显著地低的(P < 0.01 ) 。在 1 个星期的 Hs-CRP 和 NT-proBNP 的层次一种总线标准以后在组 B 比那在组 A 显著地减少了(P < 0.01 ) 。病人们被跟随在上面,导致没有回流现象和更好的预后的发生的减少为 6 个月和在组的向的发生, A 是显著地在组 B 比那降低(P < 0.05 ).Conclusion Intracoronary nitroprusside 能改进心肌的 microcirculation。 | PAN Wei WANG Lan-feng YU Jia-hui FAN Ying YANG Shu-sen ZHOU Li-jun LI Yue LI Wei-min | 2009 | Chinese Medical Journal2009，，22： | 22 |
| 18 | Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro显示文摘OBJECTIVE: To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. METHODS: MI/R injury was induced by the occlusion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and malondialdehyde (MDA). Blood samples were col- lected to determine serum levels of creatine kinase-MB (CK-MB), cardiac troponin (cTnI) and lactate dehydrogenase (LDH). Furthermore, simulatedischemia/reperfusion (SI/R) injury in vitro was established by oxygen and glucose deprivation (OGD) for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured supernatant and the levels of intracellular reactive oxygen species (ROS), SOD and MDA in cardiomyocytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS: MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnI and LDH. Additionally, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION: MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties. | Wei Quan Ying Yin Miaomiao Xi Dan Zhou Yanrong Zhu Yue Guan Chao Guo Yanhua Wang Jialin Duan Aidong Wen | 2013 | Journal of Traditional Chinese Medicine2013，33，1： | 21 |
| 19 | Expression of B7 costimulation molecules by colorectal cancer cells reduces tumorigenicity and induces anti-tumor immunity显示文摘METHODSB7genewastransfectedintomousecoloncancercellineCMT93.ThetransfectantswereselectedinDMEMcontaining800mg/LG418,andB7mole... | HU Jin Yue, WANG Sa, ZHU Jian Gao, ZHOU Guo Hua and SUN Qu Bing | 1999 | World Journal of Gastroenterology1999，5，2： | 20 |
| 20 | Seasonal variations and size distributions of water-soluble ions in atmospheric aerosols in Beijing, 2012显示文摘The characteristics of water-soluble ions in airborne particulate matter in Beijing were investigated using ion chromatography. The results showed that the total concentrations of ions were 83.7 ± 48.9 μg/m3 in spring, 54.0 ± 17.0 μg/m3 in summer, 54.1 ± 42.9 μg/m3 in autumn, and 88.8 ± 47.7 μg/m3 in winter, respectively. Furthermore, out of all the ions, NO3-,SO42-and NH4+accounted for 81.2% in spring, 78.5% in summer, 74.6% in autumn, and 76.3%in winter. Mg2+and Ca2+were mainly associated with coarse particles, with a peak that ranged from 5.8 to 9.0 μm. Na+, NH4+and Cl-had a multi-mode distribution with peaks that ranged from 0.43 to 1.1 μm and 4.7 to 9.0 μm. K+, NO3-, and SO42-were mainly associated with fine particles, with a peak that ranged from 0.65 to 2.1 μm. The concentrations of Na+, K+,Mg2+, Ca2+, NH4+, Cl-, NO3-and SO42-were 2.69, 2.32, 1.01, 4.84, 16.9, 11.8, 42.0, and 44.1 μg/m3 in particulate matter(PM) on foggy days, respectively, which were 1.4 to 7.3 times higher than those on clear days. The concentrations of these ions were 2.40, 1.66, 0.92, 4.95, 17.5,7.00, 32.6, and 34.7 μg/m3 in PM on hazy days, respectively, which were 1.2–5.7 times higher than those on clear days. | Yongjie Yang Rui Zhou Jianjun Wu Yue Yu Zhiqiang Ma Lejian Zhang Yi'an Di | 2015 | Journal of Environmental Sciences2015，27，8： | 19 |
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